Low-voltage-activated calcium channel subunit expression in a genetic model of absence epilepsy in the rat
The Genetic Absence Epilepsy Rats from Strasbourg (GAERS) are an inbred strain of rats that display many of the characteristics of human absence epilepsy. In these rats, reciprocal thalamocortical projections play a critical role in the generation of spike-and-wave discharges that characterize absen...
Gespeichert in:
| Veröffentlicht in: | Brain research. Molecular brain research. 2000-01, Vol.75 (1), p.159-165 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 165 |
|---|---|
| container_issue | 1 |
| container_start_page | 159 |
| container_title | Brain research. Molecular brain research. |
| container_volume | 75 |
| creator | Talley, Edmund M Solórzano, Guillermo Depaulis, Antoine Perez-Reyes, Edward Bayliss, Douglas A |
| description | The Genetic Absence Epilepsy Rats from Strasbourg (GAERS) are an inbred strain of rats that display many of the characteristics of human absence epilepsy. In these rats, reciprocal thalamocortical projections play a critical role in the generation of spike-and-wave discharges that characterize absence seizures. When compared to those of the non-epileptic control strain, juvenile animals of the GAERS strain reportedly possess higher-amplitude T-type calcium currents in neurons of the thalamic reticular nucleus (nRt). We hypothesized that differences in calcium currents seen between GAERS and controls result from differences in expression of genes for low-voltage-activated calcium channels. Quantitative in situ hybridization was used to compare expression of α1G, α1H, α1I, and α1E calcium channel subunit mRNAs from adult and juvenile animals of the two strains. We found higher levels of α1H mRNA expression in nRt neurons of juvenile animals (34.9±2.3 vs. 28.4±1.8 grains/10
3 pixels,
p |
| doi_str_mv | 10.1016/S0169-328X(99)00307-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70870773</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0169328X99003071</els_id><sourcerecordid>17444289</sourcerecordid><originalsourceid>FETCH-LOGICAL-c473t-13f470e221b28c172474b0ed38a36ea828b85a281cb6a2b0daa04b9e3844fd833</originalsourceid><addsrcrecordid>eNqF0c1u1DAUBWALgei08AggLxCCRcB_jZ0VQhUFpJFYABI768a5aV0ldrCdgb49ns4I2HVjb757bZ1DyDPO3nDG27df69E1Upgfr7ruNWOS6YY_IBtutGjaTvGHZPOXnJDTnG8YY9xw_piccNYq0zG2ITfb-KvZxanAFTbgit9BwYE6mJxfZ-quIQScaF77NfhC8feSMGcfA_WBAr3CgMU7OsehqjhS6DMGhxQXP-GSb_esXCNNUJ6QRyNMGZ8e7zPy_fLDt4tPzfbLx88X77eNU1qWhstRaYZC8F4Yx7VQWvUMB2lAtghGmN6cgzDc9S2Ing0ATPUdSqPUOBgpz8jLw94lxZ8r5mJnnx1OEwSMa7aaGc20vh9yrZQSpqvw_ABdijknHO2S_Azp1nJm923YuzbsPmrbdfauDcvr3PPjA2s_4_Df1CH-Cl4cAeQa-ZggOJ__OaFkrbOydweGNbadx2Sz8_uYB5_QFTtEf89P_gB2O6dB</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17444289</pqid></control><display><type>article</type><title>Low-voltage-activated calcium channel subunit expression in a genetic model of absence epilepsy in the rat</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Talley, Edmund M ; Solórzano, Guillermo ; Depaulis, Antoine ; Perez-Reyes, Edward ; Bayliss, Douglas A</creator><creatorcontrib>Talley, Edmund M ; Solórzano, Guillermo ; Depaulis, Antoine ; Perez-Reyes, Edward ; Bayliss, Douglas A</creatorcontrib><description>The Genetic Absence Epilepsy Rats from Strasbourg (GAERS) are an inbred strain of rats that display many of the characteristics of human absence epilepsy. In these rats, reciprocal thalamocortical projections play a critical role in the generation of spike-and-wave discharges that characterize absence seizures. When compared to those of the non-epileptic control strain, juvenile animals of the GAERS strain reportedly possess higher-amplitude T-type calcium currents in neurons of the thalamic reticular nucleus (nRt). We hypothesized that differences in calcium currents seen between GAERS and controls result from differences in expression of genes for low-voltage-activated calcium channels. Quantitative in situ hybridization was used to compare expression of α1G, α1H, α1I, and α1E calcium channel subunit mRNAs from adult and juvenile animals of the two strains. We found higher levels of α1H mRNA expression in nRt neurons of juvenile animals (34.9±2.3 vs. 28.4±1.8 grains/10
3 pixels,
p<0.05), perhaps accounting in part for earlier reports of elevated T-type current amplitude in those cells. In adult GAERS animals, we found elevated levels of α1G mRNA in neurons of the ventral posterior thalamic relay nuclei (64.8±3.5 vs. 53.5±1.7 grains/10
3 pixels,
p<0.05), as well as higher levels of α1H mRNA in nRt neurons (32.6±0.8 vs. 28.2±1.6 grains/10
3 pixels,
p<0.05). These results suggest that the epileptic phenotype apparent in adult GAERS may result in part from these significant, albeit small (∼15–25%), elevations in T-type calcium channel mRNA levels.</description><identifier>ISSN: 0169-328X</identifier><identifier>EISSN: 1872-6941</identifier><identifier>DOI: 10.1016/S0169-328X(99)00307-1</identifier><identifier>PMID: 10648900</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Absence epilepsy ; Aging ; Animals ; Biological and medical sciences ; Brain - growth & development ; Brain - metabolism ; Calcium channel ; Calcium Channels, T-Type - genetics ; Calcium Channels, T-Type - physiology ; Epilepsy, Absence - genetics ; Epilepsy, Absence - physiopathology ; Gene Expression Regulation, Developmental ; Headache. Facial pains. Syncopes. Epilepsia. Intracranial hypertension. Brain oedema. Cerebral palsy ; Humans ; In Situ Hybridization ; Macromolecular Substances ; Medical sciences ; Nervous system (semeiology, syndromes) ; Neurology ; Neurons - metabolism ; Rats ; Rats, Inbred Strains ; Rats, Mutant Strains ; RNA, Messenger - analysis ; RNA, Messenger - genetics ; Thalamus ; Transcription, Genetic</subject><ispartof>Brain research. Molecular brain research., 2000-01, Vol.75 (1), p.159-165</ispartof><rights>2000 Elsevier Science B.V.</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c473t-13f470e221b28c172474b0ed38a36ea828b85a281cb6a2b0daa04b9e3844fd833</citedby><cites>FETCH-LOGICAL-c473t-13f470e221b28c172474b0ed38a36ea828b85a281cb6a2b0daa04b9e3844fd833</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1243872$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10648900$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Talley, Edmund M</creatorcontrib><creatorcontrib>Solórzano, Guillermo</creatorcontrib><creatorcontrib>Depaulis, Antoine</creatorcontrib><creatorcontrib>Perez-Reyes, Edward</creatorcontrib><creatorcontrib>Bayliss, Douglas A</creatorcontrib><title>Low-voltage-activated calcium channel subunit expression in a genetic model of absence epilepsy in the rat</title><title>Brain research. Molecular brain research.</title><addtitle>Brain Res Mol Brain Res</addtitle><description>The Genetic Absence Epilepsy Rats from Strasbourg (GAERS) are an inbred strain of rats that display many of the characteristics of human absence epilepsy. In these rats, reciprocal thalamocortical projections play a critical role in the generation of spike-and-wave discharges that characterize absence seizures. When compared to those of the non-epileptic control strain, juvenile animals of the GAERS strain reportedly possess higher-amplitude T-type calcium currents in neurons of the thalamic reticular nucleus (nRt). We hypothesized that differences in calcium currents seen between GAERS and controls result from differences in expression of genes for low-voltage-activated calcium channels. Quantitative in situ hybridization was used to compare expression of α1G, α1H, α1I, and α1E calcium channel subunit mRNAs from adult and juvenile animals of the two strains. We found higher levels of α1H mRNA expression in nRt neurons of juvenile animals (34.9±2.3 vs. 28.4±1.8 grains/10
3 pixels,
p<0.05), perhaps accounting in part for earlier reports of elevated T-type current amplitude in those cells. In adult GAERS animals, we found elevated levels of α1G mRNA in neurons of the ventral posterior thalamic relay nuclei (64.8±3.5 vs. 53.5±1.7 grains/10
3 pixels,
p<0.05), as well as higher levels of α1H mRNA in nRt neurons (32.6±0.8 vs. 28.2±1.6 grains/10
3 pixels,
p<0.05). These results suggest that the epileptic phenotype apparent in adult GAERS may result in part from these significant, albeit small (∼15–25%), elevations in T-type calcium channel mRNA levels.</description><subject>Absence epilepsy</subject><subject>Aging</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Brain - growth & development</subject><subject>Brain - metabolism</subject><subject>Calcium channel</subject><subject>Calcium Channels, T-Type - genetics</subject><subject>Calcium Channels, T-Type - physiology</subject><subject>Epilepsy, Absence - genetics</subject><subject>Epilepsy, Absence - physiopathology</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Headache. Facial pains. Syncopes. Epilepsia. Intracranial hypertension. Brain oedema. Cerebral palsy</subject><subject>Humans</subject><subject>In Situ Hybridization</subject><subject>Macromolecular Substances</subject><subject>Medical sciences</subject><subject>Nervous system (semeiology, syndromes)</subject><subject>Neurology</subject><subject>Neurons - metabolism</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Rats, Mutant Strains</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Messenger - genetics</subject><subject>Thalamus</subject><subject>Transcription, Genetic</subject><issn>0169-328X</issn><issn>1872-6941</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0c1u1DAUBWALgei08AggLxCCRcB_jZ0VQhUFpJFYABI768a5aV0ldrCdgb49ns4I2HVjb757bZ1DyDPO3nDG27df69E1Upgfr7ruNWOS6YY_IBtutGjaTvGHZPOXnJDTnG8YY9xw_piccNYq0zG2ITfb-KvZxanAFTbgit9BwYE6mJxfZ-quIQScaF77NfhC8feSMGcfA_WBAr3CgMU7OsehqjhS6DMGhxQXP-GSb_esXCNNUJ6QRyNMGZ8e7zPy_fLDt4tPzfbLx88X77eNU1qWhstRaYZC8F4Yx7VQWvUMB2lAtghGmN6cgzDc9S2Ing0ATPUdSqPUOBgpz8jLw94lxZ8r5mJnnx1OEwSMa7aaGc20vh9yrZQSpqvw_ABdijknHO2S_Azp1nJm923YuzbsPmrbdfauDcvr3PPjA2s_4_Df1CH-Cl4cAeQa-ZggOJ__OaFkrbOydweGNbadx2Sz8_uYB5_QFTtEf89P_gB2O6dB</recordid><startdate>20000110</startdate><enddate>20000110</enddate><creator>Talley, Edmund M</creator><creator>Solórzano, Guillermo</creator><creator>Depaulis, Antoine</creator><creator>Perez-Reyes, Edward</creator><creator>Bayliss, Douglas A</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20000110</creationdate><title>Low-voltage-activated calcium channel subunit expression in a genetic model of absence epilepsy in the rat</title><author>Talley, Edmund M ; Solórzano, Guillermo ; Depaulis, Antoine ; Perez-Reyes, Edward ; Bayliss, Douglas A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c473t-13f470e221b28c172474b0ed38a36ea828b85a281cb6a2b0daa04b9e3844fd833</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Absence epilepsy</topic><topic>Aging</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Brain - growth & development</topic><topic>Brain - metabolism</topic><topic>Calcium channel</topic><topic>Calcium Channels, T-Type - genetics</topic><topic>Calcium Channels, T-Type - physiology</topic><topic>Epilepsy, Absence - genetics</topic><topic>Epilepsy, Absence - physiopathology</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Headache. Facial pains. Syncopes. Epilepsia. Intracranial hypertension. Brain oedema. Cerebral palsy</topic><topic>Humans</topic><topic>In Situ Hybridization</topic><topic>Macromolecular Substances</topic><topic>Medical sciences</topic><topic>Nervous system (semeiology, syndromes)</topic><topic>Neurology</topic><topic>Neurons - metabolism</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Rats, Mutant Strains</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Messenger - genetics</topic><topic>Thalamus</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Talley, Edmund M</creatorcontrib><creatorcontrib>Solórzano, Guillermo</creatorcontrib><creatorcontrib>Depaulis, Antoine</creatorcontrib><creatorcontrib>Perez-Reyes, Edward</creatorcontrib><creatorcontrib>Bayliss, Douglas A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research. Molecular brain research.</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Talley, Edmund M</au><au>Solórzano, Guillermo</au><au>Depaulis, Antoine</au><au>Perez-Reyes, Edward</au><au>Bayliss, Douglas A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Low-voltage-activated calcium channel subunit expression in a genetic model of absence epilepsy in the rat</atitle><jtitle>Brain research. Molecular brain research.</jtitle><addtitle>Brain Res Mol Brain Res</addtitle><date>2000-01-10</date><risdate>2000</risdate><volume>75</volume><issue>1</issue><spage>159</spage><epage>165</epage><pages>159-165</pages><issn>0169-328X</issn><eissn>1872-6941</eissn><abstract>The Genetic Absence Epilepsy Rats from Strasbourg (GAERS) are an inbred strain of rats that display many of the characteristics of human absence epilepsy. In these rats, reciprocal thalamocortical projections play a critical role in the generation of spike-and-wave discharges that characterize absence seizures. When compared to those of the non-epileptic control strain, juvenile animals of the GAERS strain reportedly possess higher-amplitude T-type calcium currents in neurons of the thalamic reticular nucleus (nRt). We hypothesized that differences in calcium currents seen between GAERS and controls result from differences in expression of genes for low-voltage-activated calcium channels. Quantitative in situ hybridization was used to compare expression of α1G, α1H, α1I, and α1E calcium channel subunit mRNAs from adult and juvenile animals of the two strains. We found higher levels of α1H mRNA expression in nRt neurons of juvenile animals (34.9±2.3 vs. 28.4±1.8 grains/10
3 pixels,
p<0.05), perhaps accounting in part for earlier reports of elevated T-type current amplitude in those cells. In adult GAERS animals, we found elevated levels of α1G mRNA in neurons of the ventral posterior thalamic relay nuclei (64.8±3.5 vs. 53.5±1.7 grains/10
3 pixels,
p<0.05), as well as higher levels of α1H mRNA in nRt neurons (32.6±0.8 vs. 28.2±1.6 grains/10
3 pixels,
p<0.05). These results suggest that the epileptic phenotype apparent in adult GAERS may result in part from these significant, albeit small (∼15–25%), elevations in T-type calcium channel mRNA levels.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>10648900</pmid><doi>10.1016/S0169-328X(99)00307-1</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0169-328X |
| ispartof | Brain research. Molecular brain research., 2000-01, Vol.75 (1), p.159-165 |
| issn | 0169-328X 1872-6941 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70870773 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Absence epilepsy Aging Animals Biological and medical sciences Brain - growth & development Brain - metabolism Calcium channel Calcium Channels, T-Type - genetics Calcium Channels, T-Type - physiology Epilepsy, Absence - genetics Epilepsy, Absence - physiopathology Gene Expression Regulation, Developmental Headache. Facial pains. Syncopes. Epilepsia. Intracranial hypertension. Brain oedema. Cerebral palsy Humans In Situ Hybridization Macromolecular Substances Medical sciences Nervous system (semeiology, syndromes) Neurology Neurons - metabolism Rats Rats, Inbred Strains Rats, Mutant Strains RNA, Messenger - analysis RNA, Messenger - genetics Thalamus Transcription, Genetic |
| title | Low-voltage-activated calcium channel subunit expression in a genetic model of absence epilepsy in the rat |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-21T19%3A54%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Low-voltage-activated%20calcium%20channel%20subunit%20expression%20in%20a%20genetic%20model%20of%20absence%20epilepsy%20in%20the%20rat&rft.jtitle=Brain%20research.%20Molecular%20brain%20research.&rft.au=Talley,%20Edmund%20M&rft.date=2000-01-10&rft.volume=75&rft.issue=1&rft.spage=159&rft.epage=165&rft.pages=159-165&rft.issn=0169-328X&rft.eissn=1872-6941&rft_id=info:doi/10.1016/S0169-328X(99)00307-1&rft_dat=%3Cproquest_cross%3E17444289%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17444289&rft_id=info:pmid/10648900&rft_els_id=S0169328X99003071&rfr_iscdi=true |