Release of embryogenic carrot cells with high regeneration potency from immobilized alginate beads
For the mass-production of regenerated carrot plantlets, embryogenic carrot callus immobilized in calcium alginate gel beads was cultivated in a growth medium and the regeneration frequency of cells released from alginate gel beads was compared with that in a suspension culture. Cells released in th...
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| Veröffentlicht in: | Journal of bioscience and bioengineering 1999, Vol.88 (2), p.226-228 |
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| container_end_page | 228 |
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| container_issue | 2 |
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| container_title | Journal of bioscience and bioengineering |
| container_volume | 88 |
| creator | Nagamori, Eiji Honda, Hiroyuki Kobayashi, Takeshi |
| description | For the mass-production of regenerated carrot plantlets, embryogenic carrot callus immobilized in calcium alginate gel beads was cultivated in a growth medium and the regeneration frequency of cells released from alginate gel beads was compared with that in a suspension culture. Cells released in the immobilized culture were regenerated at a frequency which was about 1.5 times higher than that obtained in the suspension culture. When CaCl
2 was added to the growth medium at 5 mM, repeated batch culture for plantlet production continued for 245 d with no significant decrease in the productivity (1.6 × 10
5 plantlets/
l-medium/d). |
| doi_str_mv | 10.1016/S1389-1723(99)80207-2 |
| format | Article |
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2 was added to the growth medium at 5 mM, repeated batch culture for plantlet production continued for 245 d with no significant decrease in the productivity (1.6 × 10
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2 was added to the growth medium at 5 mM, repeated batch culture for plantlet production continued for 245 d with no significant decrease in the productivity (1.6 × 10
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| fulltext | fulltext |
| identifier | ISSN: 1389-1723 |
| ispartof | Journal of bioscience and bioengineering, 1999, Vol.88 (2), p.226-228 |
| issn | 1389-1723 1347-4421 |
| language | eng |
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| source | Elsevier ScienceDirect Journals Complete |
| subjects | ALGINATE alginate gel beads ALGINATES ALGINATOS Biological and medical sciences Biotechnology CAL CALLO CALLUS carrot callus DAUCUS CAROTA EMBRION SOMATICO EMBRYON SOMATIQUE Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology IMMOBILISATION IMMOBILIZATION INMOVILIZACION Methods. Procedures. Technologies Miscellaneous Plant cells and fungal cells plant regeneration REGENERACION REGENERATION somatic embryo SOMATIC EMBRYOS |
| title | Release of embryogenic carrot cells with high regeneration potency from immobilized alginate beads |
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