Mapping of the ATP-binding Sites on Inositol 1,4,5-Trisphosphate Receptor Type 1 and Type 3 Homotetramers by Controlled Proteolysis and Photoaffinity Labeling

Submillimolar ATP concentrations strongly enhance the inositol 1,4,5-trisphosphate (IP3)-induced Ca2+ release, by binding specifically to ATP-binding sites on the IP3 receptor (IP3R). To locate those ATP-binding sites on IP3R1 and IP3R3, both proteins were expressed in Sf9 insect cells and covalentl...

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Veröffentlicht in:	The Journal of biological chemistry 2001-02, Vol.276 (5), p.3492-3497
Hauptverfasser:	Maes, Karlien, Missiaen, Ludwig, Parys, Jan B., De Smet, Patrick, Sienaert, Ilse, Waelkens, Etienne, Callewaert, Geert, De Smedt, Humbert
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphate - metabolism Animals Binding Sites Calcium - metabolism Calcium Channels - metabolism Cells, Cultured Inositol 1,4,5-Trisphosphate - pharmacology Inositol 1,4,5-Trisphosphate Receptors Insecta Peptide Fragments - metabolism Peptide Hydrolases - metabolism Photoaffinity Labels - metabolism Receptors, Cytoplasmic and Nuclear - metabolism
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