Hydroxylation of Limonene Enantiomers and Analogs by Recombinant (−)-Limonene 3- and 6-Hydroxylases from Mint (Mentha) Species: Evidence for Catalysis within Sterically Constrained Active Sites

Limonene enantiomers and substrate analogs, including specifically fluorinated derivatives, were utilized to probe active site interactions with recombinant (−)-(4S)-limonene-3-hydroxylase (CYP71D13) and (−)-(4S)-limonene-6-hydroxylase (CYP71D18) from mint (Mentha) species. (−)-(4S)-Limonene is hydr...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Archives of biochemistry and biophysics 2001-03, Vol.387 (1), p.125-136
Hauptverfasser:	Wüst, Matthias, Little, Dawn B, Schalk, Michel, Croteau, Rodney
Format:	Artikel
Sprache:	eng
Schlagworte:	carveol Catalytic Domain Cyclohexenes Cytochrome P-450 Enzyme System - genetics Cytochrome P-450 Enzyme System - metabolism cytochrome P450 monooxygenase fluorinated substrate analogs Fluorine Compounds - chemistry Fluorine Compounds - metabolism Hydroxylation isopiperitenol Lamiaceae Lamiaceae - enzymology Limonene Mentha piperita Mentha spicata Mixed Function Oxygenases - genetics Mixed Function Oxygenases - metabolism monoterpene biosynthesis Monoterpenes Recombinant Proteins - metabolism Stereoisomerism Substrate Specificity Terpenes - chemistry Terpenes - metabolism
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!