Enhanced Expression of Cytochrome P450s from lac-Based Plasmids Using Lactose as the Inducer

The cytochrome P450 expression systems used in Escherichia coli are highly regulated and involve the use of the lac repressor to control expression. Induction in these systems utilizes the nonmetabolizable analog of lactose, isopropyl-β-d-thiogalactopyranoside (IPTG), which is the most expensive com...

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Veröffentlicht in:	Archives of biochemistry and biophysics 2001-04, Vol.388 (2), p.276-280
Hauptverfasser:	Woyski, Denice, Cupp-Vickery, Jill R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Cytochrome P-450 Enzyme System - biosynthesis Cytochrome P-450 Enzyme System - genetics cytochrome P450s Escherichia coli expression Gene Expression Regulation, Enzymologic - drug effects IPTG Isopropyl Thiogalactoside - pharmacology lactose Lactose - pharmacology Plasmids - genetics
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