Brassica rapa Has Three Genes That Encode Proteins Associated with Different Neutral Lipids in Plastids of Specific Tissues
Plastid lipid-associated protein (PAP), a predominant structural protein associated with carotenoids and other non-green neutral lipids in plastids, was shown to be encoded by a single nuclear gene in several species. Here we report three PAP genes in the diploid Brassica rapa; the three PAPs are as...
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| Veröffentlicht in: | Plant physiology (Bethesda) 2001-05, Vol.126 (1), p.330-341 |
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| creator | Hyun Uk Kim Sherry S. H. Wu Ratnayake, Chandra Anthony H. C. Huang |
| description | Plastid lipid-associated protein (PAP), a predominant structural protein associated with carotenoids and other non-green neutral lipids in plastids, was shown to be encoded by a single nuclear gene in several species. Here we report three PAP genes in the diploid Brassica rapa; the three PAPs are associated with different lipids in specific tissues. Pap1 and Pap2 are more similar to each other (84% amino acid sequence identity) than to Pap3 (46% and 44%, respectively) in the encoded mature proteins. Pap1 transcript was most abundant in the maturing anthers (tapetum) and in lesser amounts in leaves, fruit coats, seeds, and sepals; Pap2 transcript was abundant only in the petals; and Pap3 transcript had a wide distribution, but at minimal levels in numerous organs. Immunoblotting after sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that most organs had several nanograms of PAP1 or PAP2 per milligram of total protein, the highest amounts being in the anthers (10.9 μg mg-1 PAP1) and petals (6.6 μg mg-1 PAP2), and that they had much less PAP3 ( |
| doi_str_mv | 10.1104/pp.126.1.330 |
| format | Article |
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H. Wu ; Ratnayake, Chandra ; Anthony H. C. Huang</creator><creatorcontrib>Hyun Uk Kim ; Sherry S. H. Wu ; Ratnayake, Chandra ; Anthony H. C. Huang</creatorcontrib><description>Plastid lipid-associated protein (PAP), a predominant structural protein associated with carotenoids and other non-green neutral lipids in plastids, was shown to be encoded by a single nuclear gene in several species. Here we report three PAP genes in the diploid Brassica rapa; the three PAPs are associated with different lipids in specific tissues. Pap1 and Pap2 are more similar to each other (84% amino acid sequence identity) than to Pap3 (46% and 44%, respectively) in the encoded mature proteins. Pap1 transcript was most abundant in the maturing anthers (tapetum) and in lesser amounts in leaves, fruit coats, seeds, and sepals; Pap2 transcript was abundant only in the petals; and Pap3 transcript had a wide distribution, but at minimal levels in numerous organs. Immunoblotting after sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that most organs had several nanograms of PAP1 or PAP2 per milligram of total protein, the highest amounts being in the anthers (10.9 μg mg-1 PAP1) and petals (6.6 μg mg-1 PAP2), and that they had much less PAP3 (<0.02 μg mg-1). In these organs PAP was localized in isolated plastid fractions. Plants were subjected to abiotic stresses; drought and ozone reduced the levels of the three Pap transcripts, whereas mechanical wounding and altering the light intensity enhanced their levels. We conclude that the PAP gene family consists of several members whose proteins are associated with different lipids and whose expressions are controlled by distinct mechanisms. Earlier reports of the expression of one Pap gene in various organs in a species need to be re-examined.</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.126.1.330</identifier><identifier>PMID: 11351096</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>Amino Acid Sequence ; Amino acids ; Anthers ; Base Sequence ; Biochemical Processes and Macromolecular Structures ; Biological and medical sciences ; Brassica - genetics ; Brassica - growth & development ; Brassica rapa ; Carotenoids ; Cell biochemistry ; Cell physiology ; Complementary DNA ; Diploidy ; DNA Primers ; Drought ; Fundamental and applied biological sciences. Psychology ; Genes ; Genes, Plant ; Introns ; Leaves ; Light intensity ; Lipid Metabolism ; Lipids ; Molecular Sequence Data ; Pancreatitis-Associated Proteins ; Pap1 gene ; Pap2 gene ; Pap3 gene ; Petals ; Plant physiology and development ; Plants ; Plastid lipid-associated proteins ; Plastids ; Plastids - metabolism ; Proteins ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Sequence Homology, Amino Acid</subject><ispartof>Plant physiology (Bethesda), 2001-05, Vol.126 (1), p.330-341</ispartof><rights>Copyright 2001 American Society of Plant Physiologists</rights><rights>2001 INIST-CNRS</rights><rights>Copyright American Society of Plant Physiologists May 2001</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c468t-19f955b9535073319eb3df50b02871eeda284b266a1279a727b65c6f6699b6683</citedby><cites>FETCH-LOGICAL-c468t-19f955b9535073319eb3df50b02871eeda284b266a1279a727b65c6f6699b6683</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4279891$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4279891$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>315,782,786,805,27931,27932,58024,58257</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=977847$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11351096$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hyun Uk Kim</creatorcontrib><creatorcontrib>Sherry S. H. Wu</creatorcontrib><creatorcontrib>Ratnayake, Chandra</creatorcontrib><creatorcontrib>Anthony H. C. Huang</creatorcontrib><title>Brassica rapa Has Three Genes That Encode Proteins Associated with Different Neutral Lipids in Plastids of Specific Tissues</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>Plastid lipid-associated protein (PAP), a predominant structural protein associated with carotenoids and other non-green neutral lipids in plastids, was shown to be encoded by a single nuclear gene in several species. Here we report three PAP genes in the diploid Brassica rapa; the three PAPs are associated with different lipids in specific tissues. Pap1 and Pap2 are more similar to each other (84% amino acid sequence identity) than to Pap3 (46% and 44%, respectively) in the encoded mature proteins. Pap1 transcript was most abundant in the maturing anthers (tapetum) and in lesser amounts in leaves, fruit coats, seeds, and sepals; Pap2 transcript was abundant only in the petals; and Pap3 transcript had a wide distribution, but at minimal levels in numerous organs. Immunoblotting after sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that most organs had several nanograms of PAP1 or PAP2 per milligram of total protein, the highest amounts being in the anthers (10.9 μg mg-1 PAP1) and petals (6.6 μg mg-1 PAP2), and that they had much less PAP3 (<0.02 μg mg-1). In these organs PAP was localized in isolated plastid fractions. Plants were subjected to abiotic stresses; drought and ozone reduced the levels of the three Pap transcripts, whereas mechanical wounding and altering the light intensity enhanced their levels. We conclude that the PAP gene family consists of several members whose proteins are associated with different lipids and whose expressions are controlled by distinct mechanisms. Earlier reports of the expression of one Pap gene in various organs in a species need to be re-examined.</description><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Anthers</subject><subject>Base Sequence</subject><subject>Biochemical Processes and Macromolecular Structures</subject><subject>Biological and medical sciences</subject><subject>Brassica - genetics</subject><subject>Brassica - growth & development</subject><subject>Brassica rapa</subject><subject>Carotenoids</subject><subject>Cell biochemistry</subject><subject>Cell physiology</subject><subject>Complementary DNA</subject><subject>Diploidy</subject><subject>DNA Primers</subject><subject>Drought</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genes, Plant</subject><subject>Introns</subject><subject>Leaves</subject><subject>Light intensity</subject><subject>Lipid Metabolism</subject><subject>Lipids</subject><subject>Molecular Sequence Data</subject><subject>Pancreatitis-Associated Proteins</subject><subject>Pap1 gene</subject><subject>Pap2 gene</subject><subject>Pap3 gene</subject><subject>Petals</subject><subject>Plant physiology and development</subject><subject>Plants</subject><subject>Plastid lipid-associated proteins</subject><subject>Plastids</subject><subject>Plastids - metabolism</subject><subject>Proteins</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Homology, Amino Acid</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqF0c1rFDEYBvAgit2u3jyKBAVP7pp3MpOPY21rKyxacD2HTCahWWZnpnkziPjPm2WXCl485YH8ePPxEPIK2BqA1R-naQ2VWMOac_aELKDh1apqavWULBgrmSmlz8g54o4xBhzq5-QMgDfAtFiQ35-SRYzO0mQnS28t0u198p7e-MEfss30enBj5-ldGrOPA9ILxNFFm31Hf8Z8T69iCD75IdOvfs7J9nQTp9ghjQO96y3mQx4D_T55F0N0dBsRZ48vyLNge_QvT-uS_Ph8vb28XW2-3Xy5vNisXC1UXoEOumla3fCGSc5B-5Z3oWEtq5QE7ztbqbqthLBQSW1lJVvROBGE0LoVQvEleX-cO6XxoZybzT6i831vBz_OaCRTB_t_CFJJIcodluTtP3A3zmkojzAVKFExWWpYkg9H5NKImHwwU4p7m34ZYOZQnZkmU6ozYEp1hb85zZzbve_-4lNXBbw7AYvO9iHZwUV8dFpKVcuiXh_VDvOYHnfr8jVKA_8DxfuolA</recordid><startdate>20010501</startdate><enddate>20010501</enddate><creator>Hyun Uk Kim</creator><creator>Sherry S. 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H. Wu ; Ratnayake, Chandra ; Anthony H. C. Huang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c468t-19f955b9535073319eb3df50b02871eeda284b266a1279a727b65c6f6699b6683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Anthers</topic><topic>Base Sequence</topic><topic>Biochemical Processes and Macromolecular Structures</topic><topic>Biological and medical sciences</topic><topic>Brassica - genetics</topic><topic>Brassica - growth & development</topic><topic>Brassica rapa</topic><topic>Carotenoids</topic><topic>Cell biochemistry</topic><topic>Cell physiology</topic><topic>Complementary DNA</topic><topic>Diploidy</topic><topic>DNA Primers</topic><topic>Drought</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Genes, Plant</topic><topic>Introns</topic><topic>Leaves</topic><topic>Light intensity</topic><topic>Lipid Metabolism</topic><topic>Lipids</topic><topic>Molecular Sequence Data</topic><topic>Pancreatitis-Associated Proteins</topic><topic>Pap1 gene</topic><topic>Pap2 gene</topic><topic>Pap3 gene</topic><topic>Petals</topic><topic>Plant physiology and development</topic><topic>Plants</topic><topic>Plastid lipid-associated proteins</topic><topic>Plastids</topic><topic>Plastids - metabolism</topic><topic>Proteins</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Sequence Homology, Amino Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hyun Uk Kim</creatorcontrib><creatorcontrib>Sherry S. H. Wu</creatorcontrib><creatorcontrib>Ratnayake, Chandra</creatorcontrib><creatorcontrib>Anthony H. C. 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H. Wu</au><au>Ratnayake, Chandra</au><au>Anthony H. C. Huang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Brassica rapa Has Three Genes That Encode Proteins Associated with Different Neutral Lipids in Plastids of Specific Tissues</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>2001-05-01</date><risdate>2001</risdate><volume>126</volume><issue>1</issue><spage>330</spage><epage>341</epage><pages>330-341</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>Plastid lipid-associated protein (PAP), a predominant structural protein associated with carotenoids and other non-green neutral lipids in plastids, was shown to be encoded by a single nuclear gene in several species. Here we report three PAP genes in the diploid Brassica rapa; the three PAPs are associated with different lipids in specific tissues. Pap1 and Pap2 are more similar to each other (84% amino acid sequence identity) than to Pap3 (46% and 44%, respectively) in the encoded mature proteins. Pap1 transcript was most abundant in the maturing anthers (tapetum) and in lesser amounts in leaves, fruit coats, seeds, and sepals; Pap2 transcript was abundant only in the petals; and Pap3 transcript had a wide distribution, but at minimal levels in numerous organs. Immunoblotting after sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that most organs had several nanograms of PAP1 or PAP2 per milligram of total protein, the highest amounts being in the anthers (10.9 μg mg-1 PAP1) and petals (6.6 μg mg-1 PAP2), and that they had much less PAP3 (<0.02 μg mg-1). In these organs PAP was localized in isolated plastid fractions. Plants were subjected to abiotic stresses; drought and ozone reduced the levels of the three Pap transcripts, whereas mechanical wounding and altering the light intensity enhanced their levels. We conclude that the PAP gene family consists of several members whose proteins are associated with different lipids and whose expressions are controlled by distinct mechanisms. Earlier reports of the expression of one Pap gene in various organs in a species need to be re-examined.</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>11351096</pmid><doi>10.1104/pp.126.1.330</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Plant physiology (Bethesda), 2001-05, Vol.126 (1), p.330-341 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; JSTOR Archive Collection A-Z Listing; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Amino Acid Sequence Amino acids Anthers Base Sequence Biochemical Processes and Macromolecular Structures Biological and medical sciences Brassica - genetics Brassica - growth & development Brassica rapa Carotenoids Cell biochemistry Cell physiology Complementary DNA Diploidy DNA Primers Drought Fundamental and applied biological sciences. Psychology Genes Genes, Plant Introns Leaves Light intensity Lipid Metabolism Lipids Molecular Sequence Data Pancreatitis-Associated Proteins Pap1 gene Pap2 gene Pap3 gene Petals Plant physiology and development Plants Plastid lipid-associated proteins Plastids Plastids - metabolism Proteins RNA, Messenger - genetics RNA, Messenger - metabolism Sequence Homology, Amino Acid |
| title | Brassica rapa Has Three Genes That Encode Proteins Associated with Different Neutral Lipids in Plastids of Specific Tissues |
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