Constitutive promoter modules for PCR‐based gene modification in Saccharomyces cerevisiae
Initial steps in investigating gene function often include deleting and overexpressing the gene of interest and identifying the subcellular location of the gene product. To facilitate these procedures, a number of new PCR modules, which contain selectable markers and in some cases other genetic elem...
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| Veröffentlicht in: | Yeast (Chichester, England) England), 2001-06, Vol.18 (8), p.723-728 |
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| creator | DeMarini, Douglas J. Carlin, Edward M. Livi, George P. |
| description | Initial steps in investigating gene function often include deleting and overexpressing the gene of interest and identifying the subcellular location of the gene product. To facilitate these procedures, a number of new PCR modules, which contain selectable markers and in some cases other genetic elements (e.g promoter elements, epitope tags, and reporter genes) have been developed. These modules are used as PCR substrates to create products that can be targeted to specified locations in the yeast genome, thus modifying that genomic locus. We describe here a series of plasmids that contain a truncated version of the strong ADH1 promoter with and without amino‐terminal 3HA and GST tags. Because these plasmids contain the same vector sequences as the GAL1 promoter plasmids, a constitutive and an inducible promoter can now be integrated with a minimal number of primers. Copyright © 2001 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/yea.721 |
| format | Article |
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Copyright © 2001 John Wiley & Sons, Ltd.</description><subject>ADH1 gene</subject><subject>epitope tagging</subject><subject>functional analysis</subject><subject>GAL1 gene</subject><subject>Gene Expression</subject><subject>Genes, Fungal</subject><subject>overexpression studies</subject><subject>Plasmids</subject><subject>Polymerase Chain Reaction</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><issn>0749-503X</issn><issn>1097-0061</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtKw0AUhgdRbK3iG0hWupDUmSTNzCxLqBcoKF5AcRFOZk50JJeaSSrZ-Qg-o0_ilBZciasD53z_z-Ej5JDRMaM0OOsRxjxgW2TIqOQ-pTHbJkPKI-lPaPg4IHvWvlHK2CQQu2TAWMiFkHJInpO6sq1pu9Ys0Vs0dVm32HhlrbsCrZfXjXeT3H5_fmVgUXsvWOHqaHKjoDV15ZnKuwOlXsFFe-UiChtcGmsA98lODoXFg80ckYfz2X1y6c-vL66S6dxXYRQxH1gY5CLWMo5gonIdQw5CoBIs1pGCTEsNEGjIRMBpyN2O61hQPlFRJlWowxE5Xve69987tG1aGquwKKDCurMppyKmMpL_goxLp4zHDjxZg6qprW0wTxeNKaHpU0bTlfDUCU-dcEcebSq7rET9y20MO-B0DXyYAvu_etKn2XRV9wMIpIwd</recordid><startdate>20010601</startdate><enddate>20010601</enddate><creator>DeMarini, Douglas J.</creator><creator>Carlin, Edward M.</creator><creator>Livi, George P.</creator><general>John Wiley & Sons, Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20010601</creationdate><title>Constitutive promoter modules for PCR‐based gene modification in Saccharomyces cerevisiae</title><author>DeMarini, Douglas J. ; Carlin, Edward M. ; Livi, George P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3441-a132f86d964a5cfd6afa88ec816d4cabd9daa2dab827037d4c7d68075c4b9c3d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>ADH1 gene</topic><topic>epitope tagging</topic><topic>functional analysis</topic><topic>GAL1 gene</topic><topic>Gene Expression</topic><topic>Genes, Fungal</topic><topic>overexpression studies</topic><topic>Plasmids</topic><topic>Polymerase Chain Reaction</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DeMarini, Douglas J.</creatorcontrib><creatorcontrib>Carlin, Edward M.</creatorcontrib><creatorcontrib>Livi, George P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Yeast (Chichester, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>DeMarini, Douglas J.</au><au>Carlin, Edward M.</au><au>Livi, George P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Constitutive promoter modules for PCR‐based gene modification in Saccharomyces cerevisiae</atitle><jtitle>Yeast (Chichester, England)</jtitle><addtitle>Yeast</addtitle><date>2001-06-01</date><risdate>2001</risdate><volume>18</volume><issue>8</issue><spage>723</spage><epage>728</epage><pages>723-728</pages><issn>0749-503X</issn><eissn>1097-0061</eissn><abstract>Initial steps in investigating gene function often include deleting and overexpressing the gene of interest and identifying the subcellular location of the gene product. 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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Wiley Online Library Free Content; EZB-FREE-00999 freely available EZB journals |
| subjects | ADH1 gene epitope tagging functional analysis GAL1 gene Gene Expression Genes, Fungal overexpression studies Plasmids Polymerase Chain Reaction Promoter Regions, Genetic - genetics Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics |
| title | Constitutive promoter modules for PCR‐based gene modification in Saccharomyces cerevisiae |
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