Analysis of amino acids in human serum by isocratic reversed-phase high-performance liquid chromatography with electrochemical detection
A simple, sensitive and reproducible isocratic high-performance liquid chromatography (HPLC) method has been developed for the determination of amino acids in human serum. The method involves precipitation of the serum proteins with methanol followed by pre-column derivatization of amino acids with...
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| Veröffentlicht in: | Journal of Chromatography A 2001-04, Vol.913 (1), p.303-308 |
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| container_title | Journal of Chromatography A |
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| creator | Tcherkas, Y.V. Kartsova, L.A. Krasnova, I.N. |
| description | A simple, sensitive and reproducible isocratic high-performance liquid chromatography (HPLC) method has been developed for the determination of amino acids in human serum. The method involves precipitation of the serum proteins with methanol followed by pre-column derivatization of amino acids with
o-phthalaldehyde–2-mercaptoethanol or
o-phthalaldehyde–sodium sulfite. HPLC separation of the derivatives was performed using an ODS column with an isocratic mobile phase system and electrochemical detection (+0.75 V). The response was linear over the range 5–300 μ
M for all amino acids. The method allows quantitative determination of glutamic acid, asparagine, serine, glutamine, histidine, taurine, alanine, arginine, methionine, isoleucine, ornithine, leucine, phenylalanine, lysine and tryptophan at concentrations as low as 0.5–5.0 pmol (signal-to-noise ratio=2). Using this method, the levels of amino acids in serum from healthy donors and patients with ischemic stroke were determined. |
| doi_str_mv | 10.1016/S0021-9673(00)01206-1 |
| format | Article |
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o-phthalaldehyde–2-mercaptoethanol or
o-phthalaldehyde–sodium sulfite. HPLC separation of the derivatives was performed using an ODS column with an isocratic mobile phase system and electrochemical detection (+0.75 V). The response was linear over the range 5–300 μ
M for all amino acids. The method allows quantitative determination of glutamic acid, asparagine, serine, glutamine, histidine, taurine, alanine, arginine, methionine, isoleucine, ornithine, leucine, phenylalanine, lysine and tryptophan at concentrations as low as 0.5–5.0 pmol (signal-to-noise ratio=2). Using this method, the levels of amino acids in serum from healthy donors and patients with ischemic stroke were determined.</description><identifier>ISSN: 0021-9673</identifier><identifier>DOI: 10.1016/S0021-9673(00)01206-1</identifier><identifier>PMID: 11355826</identifier><identifier>CODEN: JOCRAM</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Amino acids ; Amino Acids - blood ; Blood Proteins - chemistry ; Chromatography, High Pressure Liquid - methods ; Electrochemistry ; Humans ; Reference Standards ; Reproducibility of Results ; Sensitivity and Specificity</subject><ispartof>Journal of Chromatography A, 2001-04, Vol.913 (1), p.303-308</ispartof><rights>2001 Elsevier Science B.V.</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c304t-69135b8d0f3f60eb00a4529856a3e4738433e440c7458c9341989a4a8df050883</citedby><cites>FETCH-LOGICAL-c304t-69135b8d0f3f60eb00a4529856a3e4738433e440c7458c9341989a4a8df050883</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0021-9673(00)01206-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,780,784,789,790,3548,23928,23929,25138,27922,27923,45993</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=962692$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11355826$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tcherkas, Y.V.</creatorcontrib><creatorcontrib>Kartsova, L.A.</creatorcontrib><creatorcontrib>Krasnova, I.N.</creatorcontrib><title>Analysis of amino acids in human serum by isocratic reversed-phase high-performance liquid chromatography with electrochemical detection</title><title>Journal of Chromatography A</title><addtitle>J Chromatogr A</addtitle><description>A simple, sensitive and reproducible isocratic high-performance liquid chromatography (HPLC) method has been developed for the determination of amino acids in human serum. The method involves precipitation of the serum proteins with methanol followed by pre-column derivatization of amino acids with
o-phthalaldehyde–2-mercaptoethanol or
o-phthalaldehyde–sodium sulfite. HPLC separation of the derivatives was performed using an ODS column with an isocratic mobile phase system and electrochemical detection (+0.75 V). The response was linear over the range 5–300 μ
M for all amino acids. The method allows quantitative determination of glutamic acid, asparagine, serine, glutamine, histidine, taurine, alanine, arginine, methionine, isoleucine, ornithine, leucine, phenylalanine, lysine and tryptophan at concentrations as low as 0.5–5.0 pmol (signal-to-noise ratio=2). Using this method, the levels of amino acids in serum from healthy donors and patients with ischemic stroke were determined.</description><subject>Amino acids</subject><subject>Amino Acids - blood</subject><subject>Blood Proteins - chemistry</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Electrochemistry</subject><subject>Humans</subject><subject>Reference Standards</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><issn>0021-9673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1u1TAQRr0A0VJ4BJAlJASLwDh2HGeFqoo_qRILYG352pNmUBKndlJ034DHxrf3qixZjfTpfDOjw9gLAe8ECP3-O0Atqk638g3AWxA16Eo8YucP8Rl7mvMvANFCWz9hZ0LIpjG1Pmd_Lmc37jNlHnvuJpojd55C5jTzYZvczDOmbeK7PaccfXIreZ7wDlPGUC2Dy8gHuhmqBVMfUyl45CPdbhS4H1Kc3BpvkluGPf9N68BxRL-m6AecyLuRB1xLQHF-xh73bsz4_DQv2M9PH39cfamuv33-enV5XXkJaq10V17fmQC97DXgDsCppu5Mo51E1UqjZJkKfKsa4zupRGc6p5wJPTRgjLxgr497lxRvN8yrnSh7HEc3Y9yybcFoULIuYHMEfYo5J-ztkmhyaW8F2IN2e6_dHvxaAHuv3YrSe3k6sO0mDP9aJ-cFeHUCXC4K-lScUX7gOl3r7nD-w5HCIuOOMNnsCYvdQKkYsyHSfx75C7PAojI</recordid><startdate>20010413</startdate><enddate>20010413</enddate><creator>Tcherkas, Y.V.</creator><creator>Kartsova, L.A.</creator><creator>Krasnova, I.N.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010413</creationdate><title>Analysis of amino acids in human serum by isocratic reversed-phase high-performance liquid chromatography with electrochemical detection</title><author>Tcherkas, Y.V. ; Kartsova, L.A. ; Krasnova, I.N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c304t-69135b8d0f3f60eb00a4529856a3e4738433e440c7458c9341989a4a8df050883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Amino acids</topic><topic>Amino Acids - blood</topic><topic>Blood Proteins - chemistry</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Electrochemistry</topic><topic>Humans</topic><topic>Reference Standards</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tcherkas, Y.V.</creatorcontrib><creatorcontrib>Kartsova, L.A.</creatorcontrib><creatorcontrib>Krasnova, I.N.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tcherkas, Y.V.</au><au>Kartsova, L.A.</au><au>Krasnova, I.N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of amino acids in human serum by isocratic reversed-phase high-performance liquid chromatography with electrochemical detection</atitle><jtitle>Journal of Chromatography A</jtitle><addtitle>J Chromatogr A</addtitle><date>2001-04-13</date><risdate>2001</risdate><volume>913</volume><issue>1</issue><spage>303</spage><epage>308</epage><pages>303-308</pages><issn>0021-9673</issn><coden>JOCRAM</coden><abstract>A simple, sensitive and reproducible isocratic high-performance liquid chromatography (HPLC) method has been developed for the determination of amino acids in human serum. The method involves precipitation of the serum proteins with methanol followed by pre-column derivatization of amino acids with
o-phthalaldehyde–2-mercaptoethanol or
o-phthalaldehyde–sodium sulfite. HPLC separation of the derivatives was performed using an ODS column with an isocratic mobile phase system and electrochemical detection (+0.75 V). The response was linear over the range 5–300 μ
M for all amino acids. The method allows quantitative determination of glutamic acid, asparagine, serine, glutamine, histidine, taurine, alanine, arginine, methionine, isoleucine, ornithine, leucine, phenylalanine, lysine and tryptophan at concentrations as low as 0.5–5.0 pmol (signal-to-noise ratio=2). Using this method, the levels of amino acids in serum from healthy donors and patients with ischemic stroke were determined.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>11355826</pmid><doi>10.1016/S0021-9673(00)01206-1</doi><tpages>6</tpages></addata></record> |
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| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Amino acids Amino Acids - blood Blood Proteins - chemistry Chromatography, High Pressure Liquid - methods Electrochemistry Humans Reference Standards Reproducibility of Results Sensitivity and Specificity |
| title | Analysis of amino acids in human serum by isocratic reversed-phase high-performance liquid chromatography with electrochemical detection |
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