A novel vacuolar protein encoded by SSU21 / MCD4 is involved in cell wall integrity in yeast
Using a screening procedure for obtaining yeast strains with enhanced ability to secrete heterologous protein, we have isolated a mutant with alteration of the cell wall structure. This mutant displayed strong decrease in cell wall mannoprotein content, which was not accompanied by decreased glycosy...
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| Veröffentlicht in: | Yeast (Chichester, England) England), 1999-10, Vol.15 (14), p.1485-1501 |
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| container_title | Yeast (Chichester, England) |
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| creator | Packeiser, Anna N. Urakov, Valery N. Polyakova, Yulia A. Shimanova, Natalia I. Shcherbukhin, Vladimir D. Smirnov, Vladimir N. Ter‐Avanesyan, Michael D. |
| description | Using a screening procedure for obtaining yeast strains with enhanced ability to secrete heterologous protein, we have isolated a mutant with alteration of the cell wall structure. This mutant displayed strong decrease in cell wall mannoprotein content, which was not accompanied by decreased glycosylation of secreted proteins. The mutation defines a gene, designated SSU21(identical to previously characterized MCD4), which encodes a novel vacuolar protein. SSU21 is probably connected to the cell integrity protein kinase C‐mediated pathway, since ssu21 and pkc1Δ double mutant is synthetic lethal. To our knowledge, this is the first example of a yeast vacuolar protein whose alteration results in a cell wall defect. Copyright © 1999 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/(SICI)1097-0061(199910)15:14<1485::AID-YEA477>3.0.CO;2-4 |
| format | Article |
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This mutant displayed strong decrease in cell wall mannoprotein content, which was not accompanied by decreased glycosylation of secreted proteins. The mutation defines a gene, designated SSU21(identical to previously characterized MCD4), which encodes a novel vacuolar protein. SSU21 is probably connected to the cell integrity protein kinase C‐mediated pathway, since ssu21 and pkc1Δ double mutant is synthetic lethal. To our knowledge, this is the first example of a yeast vacuolar protein whose alteration results in a cell wall defect. Copyright © 1999 John Wiley & Sons, Ltd.</description><identifier>ISSN: 0749-503X</identifier><identifier>EISSN: 1097-0061</identifier><identifier>DOI: 10.1002/(SICI)1097-0061(199910)15:14<1485::AID-YEA477>3.0.CO;2-4</identifier><identifier>PMID: 10514566</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>caffeine ; cell wall ; Cell Wall - chemistry ; Fungal Proteins - physiology ; Genes, Fungal ; lytic mutants ; MCD4 gene ; Membrane Proteins - analysis ; Membrane Proteins - genetics ; Membrane Proteins - physiology ; Mutation ; protein kinase C ; Protein Kinase C - physiology ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - chemistry ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae Proteins ; SSU21 gene ; Urokinase-Type Plasminogen Activator - metabolism ; vacuole ; Vacuoles - chemistry ; yeast</subject><ispartof>Yeast (Chichester, England), 1999-10, Vol.15 (14), p.1485-1501</ispartof><rights>Copyright © 1999 John Wiley & Sons, Ltd.</rights><rights>Copyright 1999 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F%28SICI%291097-0061%28199910%2915%3A14%3C1485%3A%3AAID-YEA477%3E3.0.CO%3B2-4$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F%28SICI%291097-0061%28199910%2915%3A14%3C1485%3A%3AAID-YEA477%3E3.0.CO%3B2-4$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10514566$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Packeiser, Anna N.</creatorcontrib><creatorcontrib>Urakov, Valery N.</creatorcontrib><creatorcontrib>Polyakova, Yulia A.</creatorcontrib><creatorcontrib>Shimanova, Natalia I.</creatorcontrib><creatorcontrib>Shcherbukhin, Vladimir D.</creatorcontrib><creatorcontrib>Smirnov, Vladimir N.</creatorcontrib><creatorcontrib>Ter‐Avanesyan, Michael D.</creatorcontrib><title>A novel vacuolar protein encoded by SSU21 / MCD4 is involved in cell wall integrity in yeast</title><title>Yeast (Chichester, England)</title><addtitle>Yeast</addtitle><description>Using a screening procedure for obtaining yeast strains with enhanced ability to secrete heterologous protein, we have isolated a mutant with alteration of the cell wall structure. 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Copyright © 1999 John Wiley & Sons, Ltd.</description><subject>caffeine</subject><subject>cell wall</subject><subject>Cell Wall - chemistry</subject><subject>Fungal Proteins - physiology</subject><subject>Genes, Fungal</subject><subject>lytic mutants</subject><subject>MCD4 gene</subject><subject>Membrane Proteins - analysis</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - physiology</subject><subject>Mutation</subject><subject>protein kinase C</subject><subject>Protein Kinase C - physiology</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - chemistry</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae Proteins</subject><subject>SSU21 gene</subject><subject>Urokinase-Type Plasminogen Activator - metabolism</subject><subject>vacuole</subject><subject>Vacuoles - chemistry</subject><subject>yeast</subject><issn>0749-503X</issn><issn>1097-0061</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkd-L00AQgBdRvN7pvyD7JHcP6c3sj2y2ilhypxZO-lAPVIRhk91IJE1qkvbIf38JreKbLzMw880wzMfYe4Q5Aojry80qXV0hWBMBxHiJ1lqEK9QLVG9RJXqxWK5uom-3S2XMOzmHebp-IyL1hM3-Dj1lMzDKRhrk1zN23nW_ABC1SJ6zMwSNSsfxjP1Y8ro5hIofXL5vKtfyXdv0oax5qPPGB8-zgW829wL5Nf-c3ihedrysD011GHsjloeq4g9uDGXdh59t2Q9TeQiu61-wZ4WruvDylC_Y_YfbL-mn6G79cZUu76KdFGiiJEcHWWIwOF9IiHMPmHmZSJ975ZLCFLGXReK9kUkBzjthtYitzJzKpUUtL9jr497x9t_70PW0LbvpMFeHZt-RgUTbRKv_gmgUCCnsCL46gftsGzzt2nLr2oH-PG4Evh-Bh7IKwz99mgTS5I8mFTSpoKM_Qk2oaPJHoz466iNJQOmaBKlTRT4CUWeRew</recordid><startdate>199910</startdate><enddate>199910</enddate><creator>Packeiser, Anna N.</creator><creator>Urakov, Valery N.</creator><creator>Polyakova, Yulia A.</creator><creator>Shimanova, Natalia I.</creator><creator>Shcherbukhin, Vladimir D.</creator><creator>Smirnov, Vladimir N.</creator><creator>Ter‐Avanesyan, Michael D.</creator><general>John Wiley & Sons, Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>199910</creationdate><title>A novel vacuolar protein encoded by SSU21 / MCD4 is involved in cell wall integrity in yeast</title><author>Packeiser, Anna N. ; Urakov, Valery N. ; Polyakova, Yulia A. ; Shimanova, Natalia I. ; Shcherbukhin, Vladimir D. ; Smirnov, Vladimir N. ; Ter‐Avanesyan, Michael D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p3217-8c1a0b871eadf306cd01bd383dcd4a8f7f6d3f8dd738f0ada2952693ba4c39153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>caffeine</topic><topic>cell wall</topic><topic>Cell Wall - chemistry</topic><topic>Fungal Proteins - physiology</topic><topic>Genes, Fungal</topic><topic>lytic mutants</topic><topic>MCD4 gene</topic><topic>Membrane Proteins - analysis</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Proteins - physiology</topic><topic>Mutation</topic><topic>protein kinase C</topic><topic>Protein Kinase C - physiology</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - chemistry</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae Proteins</topic><topic>SSU21 gene</topic><topic>Urokinase-Type Plasminogen Activator - metabolism</topic><topic>vacuole</topic><topic>Vacuoles - chemistry</topic><topic>yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Packeiser, Anna N.</creatorcontrib><creatorcontrib>Urakov, Valery N.</creatorcontrib><creatorcontrib>Polyakova, Yulia A.</creatorcontrib><creatorcontrib>Shimanova, Natalia I.</creatorcontrib><creatorcontrib>Shcherbukhin, Vladimir D.</creatorcontrib><creatorcontrib>Smirnov, Vladimir N.</creatorcontrib><creatorcontrib>Ter‐Avanesyan, Michael D.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Yeast (Chichester, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Packeiser, Anna N.</au><au>Urakov, Valery N.</au><au>Polyakova, Yulia A.</au><au>Shimanova, Natalia I.</au><au>Shcherbukhin, Vladimir D.</au><au>Smirnov, Vladimir N.</au><au>Ter‐Avanesyan, Michael D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel vacuolar protein encoded by SSU21 / MCD4 is involved in cell wall integrity in yeast</atitle><jtitle>Yeast (Chichester, England)</jtitle><addtitle>Yeast</addtitle><date>1999-10</date><risdate>1999</risdate><volume>15</volume><issue>14</issue><spage>1485</spage><epage>1501</epage><pages>1485-1501</pages><issn>0749-503X</issn><eissn>1097-0061</eissn><abstract>Using a screening procedure for obtaining yeast strains with enhanced ability to secrete heterologous protein, we have isolated a mutant with alteration of the cell wall structure. This mutant displayed strong decrease in cell wall mannoprotein content, which was not accompanied by decreased glycosylation of secreted proteins. The mutation defines a gene, designated SSU21(identical to previously characterized MCD4), which encodes a novel vacuolar protein. SSU21 is probably connected to the cell integrity protein kinase C‐mediated pathway, since ssu21 and pkc1Δ double mutant is synthetic lethal. To our knowledge, this is the first example of a yeast vacuolar protein whose alteration results in a cell wall defect. Copyright © 1999 John Wiley & Sons, Ltd.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>10514566</pmid><doi>10.1002/(SICI)1097-0061(199910)15:14<1485::AID-YEA477>3.0.CO;2-4</doi><tpages>17</tpages></addata></record> |
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| source | MEDLINE; Access via Wiley Online Library; EZB-FREE-00999 freely available EZB journals; Wiley Online Library (Open Access Collection) |
| subjects | caffeine cell wall Cell Wall - chemistry Fungal Proteins - physiology Genes, Fungal lytic mutants MCD4 gene Membrane Proteins - analysis Membrane Proteins - genetics Membrane Proteins - physiology Mutation protein kinase C Protein Kinase C - physiology Saccharomyces cerevisiae Saccharomyces cerevisiae - chemistry Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae Proteins SSU21 gene Urokinase-Type Plasminogen Activator - metabolism vacuole Vacuoles - chemistry yeast |
| title | A novel vacuolar protein encoded by SSU21 / MCD4 is involved in cell wall integrity in yeast |
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