Expression of hepatitis B surface antigen in the methylotrophic yeast Pichia pastoris using the GAP promoter

High-level expression and efficient assembly of Hepatitis B surface Antigen (HBsAg) particles have been reported in Pichia pastoris by integrating a single copy of the HBsAg gene under the control of the alcohol oxidase ( AOX1) promoter. However, the time taken to reach peak product concentration is...

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Veröffentlicht in:	Journal of biotechnology 2001-06, Vol.88 (1), p.21-35
Hauptverfasser:	Vassileva, Ana, Chugh, Dipti A, Swaminathan, Sathyamangalam, Khanna, Navin
Format:	Artikel
Sprache:	eng
Schlagworte:	Alcohol Oxidoreductases - genetics Biological and medical sciences Biotechnology Fermentation Fundamental and applied biological sciences. Psychology GAP promoter Gene Dosage Gene Expression Regulation, Fungal glyceraldehyde-3-phosphate dehydrogenase Glyceraldehyde-3-Phosphate Dehydrogenases - genetics Health. Pharmaceutical industry Hepatitis B surface antigen Hepatitis B Surface Antigens - genetics Hepatitis B Surface Antigens - metabolism Industrial applications and implications. Economical aspects Mating Factor Peptides - genetics Pichia - genetics Pichia - metabolism Pichia pastoris Production of active biomolecules Promoter Regions, Genetic Protein Engineering - methods Recombinant Proteins - genetics Recombinant Proteins - metabolism Recombinant Proteins - secretion Vaccins
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