Mesothelial regeneration is not dependent on subserosal cells
It has been proposed that after mesothelial injury, resident cells within the subserosal connective tissue proliferate, differentiate, and migrate to the serosal surface. The aim of this study was to examine the temporal and spatial changes of proliferating cells in a murine model of testicular meso...
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Veröffentlicht in: | The Journal of pathology 2000-01, Vol.190 (1), p.86-92 |
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description | It has been proposed that after mesothelial injury, resident cells within the subserosal connective tissue proliferate, differentiate, and migrate to the serosal surface. The aim of this study was to examine the temporal and spatial changes of proliferating cells in a murine model of testicular mesothelial healing and assess the potential of submesothelial cells to reconstitute the damaged mesothelium. Histology and autoradiography were employed to determine the number of cells within the submesothelial connective tissue, as well as the proportion of cells undergoing DNA synthesis on and beneath the injured serosa. Mesothelial cells surrounding the wound demonstrated maximal DNA synthesis 48 h after injury (27.82±5.64% SEM, compared with 0.17±0.16% 3H‐TdR labelled cells for resting mesothelium), whereas a significant increase in proliferating submesothelial cells was not seen until day 4 post‐injury (7.79±3.31% compared with 0.85±0.64% 3H‐TdR labelled cells at day 2). Furthermore, this small number of dividing submesothelial cells must include cells other than the proposed mesothelial precursors, indicating a very low proportion of precursor cells in the submesothelial cell population. As large numbers of mesothelial cells were seen at the wound centre by 3–4 days after injury, it is unlikely that submesothelial cells contributed significantly to the repopulation of the injured mesothelium. It is hypothesized that regenerating mesothelium is more likely to originate from the surrounding uninjured mesothelial cell population. Copyright © 2000 John Wiley & Sons, Ltd. |
doi_str_mv | 10.1002/(SICI)1096-9896(200001)190:1<86::AID-PATH493>3.0.CO;2-G |
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The aim of this study was to examine the temporal and spatial changes of proliferating cells in a murine model of testicular mesothelial healing and assess the potential of submesothelial cells to reconstitute the damaged mesothelium. Histology and autoradiography were employed to determine the number of cells within the submesothelial connective tissue, as well as the proportion of cells undergoing DNA synthesis on and beneath the injured serosa. Mesothelial cells surrounding the wound demonstrated maximal DNA synthesis 48 h after injury (27.82±5.64% SEM, compared with 0.17±0.16% 3H‐TdR labelled cells for resting mesothelium), whereas a significant increase in proliferating submesothelial cells was not seen until day 4 post‐injury (7.79±3.31% compared with 0.85±0.64% 3H‐TdR labelled cells at day 2). Furthermore, this small number of dividing submesothelial cells must include cells other than the proposed mesothelial precursors, indicating a very low proportion of precursor cells in the submesothelial cell population. As large numbers of mesothelial cells were seen at the wound centre by 3–4 days after injury, it is unlikely that submesothelial cells contributed significantly to the repopulation of the injured mesothelium. It is hypothesized that regenerating mesothelium is more likely to originate from the surrounding uninjured mesothelial cell population. Copyright © 2000 John Wiley & Sons, Ltd.</description><identifier>ISSN: 0022-3417</identifier><identifier>EISSN: 1096-9896</identifier><identifier>DOI: 10.1002/(SICI)1096-9896(200001)190:1<86::AID-PATH493>3.0.CO;2-G</identifier><identifier>PMID: 10640997</identifier><identifier>CODEN: JPTLAS</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Animals ; Autoradiography ; Biological and medical sciences ; Cell Count ; Cell Division ; Degeneration. Regeneration. Wound healing. Graft ; DNA - biosynthesis ; Epithelium - pathology ; Epithelium - physiology ; Fundamental and applied biological sciences. Psychology ; Male ; mesothelium ; Mice ; Mice, Inbred BALB C ; mouse ; precursor ; Regeneration - physiology ; Testis - physiology ; Vertebrates: anatomy and physiology, studies on body, several organs or systems ; wound healing ; Wound Healing - physiology</subject><ispartof>The Journal of pathology, 2000-01, Vol.190 (1), p.86-92</ispartof><rights>Copyright © 2000 John Wiley & Sons, Ltd.</rights><rights>2000 INIST-CNRS</rights><rights>Copyright 2000 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c4373-85f277c3020175f101064659e738574e550e85a4300856bd224470e13c87eba93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F%28SICI%291096-9896%28200001%29190%3A1%3C86%3A%3AAID-PATH493%3E3.0.CO%3B2-G$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F%28SICI%291096-9896%28200001%29190%3A1%3C86%3A%3AAID-PATH493%3E3.0.CO%3B2-G$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,4024,27923,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1242373$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10640997$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mutsaers, Steven E.</creatorcontrib><creatorcontrib>Whitaker, Darrel</creatorcontrib><creatorcontrib>Papadimitriou, John M.</creatorcontrib><title>Mesothelial regeneration is not dependent on subserosal cells</title><title>The Journal of pathology</title><addtitle>J. Pathol</addtitle><description>It has been proposed that after mesothelial injury, resident cells within the subserosal connective tissue proliferate, differentiate, and migrate to the serosal surface. The aim of this study was to examine the temporal and spatial changes of proliferating cells in a murine model of testicular mesothelial healing and assess the potential of submesothelial cells to reconstitute the damaged mesothelium. Histology and autoradiography were employed to determine the number of cells within the submesothelial connective tissue, as well as the proportion of cells undergoing DNA synthesis on and beneath the injured serosa. Mesothelial cells surrounding the wound demonstrated maximal DNA synthesis 48 h after injury (27.82±5.64% SEM, compared with 0.17±0.16% 3H‐TdR labelled cells for resting mesothelium), whereas a significant increase in proliferating submesothelial cells was not seen until day 4 post‐injury (7.79±3.31% compared with 0.85±0.64% 3H‐TdR labelled cells at day 2). Furthermore, this small number of dividing submesothelial cells must include cells other than the proposed mesothelial precursors, indicating a very low proportion of precursor cells in the submesothelial cell population. As large numbers of mesothelial cells were seen at the wound centre by 3–4 days after injury, it is unlikely that submesothelial cells contributed significantly to the repopulation of the injured mesothelium. It is hypothesized that regenerating mesothelium is more likely to originate from the surrounding uninjured mesothelial cell population. Copyright © 2000 John Wiley & Sons, Ltd.</description><subject>Animals</subject><subject>Autoradiography</subject><subject>Biological and medical sciences</subject><subject>Cell Count</subject><subject>Cell Division</subject><subject>Degeneration. Regeneration. Wound healing. Graft</subject><subject>DNA - biosynthesis</subject><subject>Epithelium - pathology</subject><subject>Epithelium - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Male</subject><subject>mesothelium</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>mouse</subject><subject>precursor</subject><subject>Regeneration - physiology</subject><subject>Testis - physiology</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><subject>wound healing</subject><subject>Wound Healing - physiology</subject><issn>0022-3417</issn><issn>1096-9896</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkF1v0zAUhi0EYmXwF1AuENouUvwZ2-VLVYC20kaRVhji5shNT0YgTYqdCvbvcZQykEDCN7aOHr9-_RDyktExo5Q_OblY5ItTRm2WWmOzE07jYqfM0gl7ZrLJZLp4lb6brubSihdiTMf58ilPZ7fI6ObObTKKSTwVkukjci-ELzHCWqXukiNGMxnPekSen2Nou89YV65OPF5hg951VdskVUiatks2uMNmg02XxFnYrwP6NkS2wLoO98md0tUBHxz2Y_L-zetVPk_PlrNFPj1LCym0SI0qudaFoJwyrUpG-_czZVELo7REpSga5aSg1KhsveFcSk2RicJoXDsrjsnjIXfn2297DB1sq9A3cA22-wA63pPW8AheDmARWwaPJex8tXX-GhiF3ixAbxZ6S9BbgsEsRLPAwGQA0SwczIIACvkSOMxi8sNDhf16i5s_cgeVEXh0AFwoXF161xRV-M1xyaOLiH0asO9Vjdd_1ftPu3-X-zWK4ekQXoUOf9yEO_8VMi20gsu3M_h4LvmH1cU8fuonU6-uiQ</recordid><startdate>200001</startdate><enddate>200001</enddate><creator>Mutsaers, Steven E.</creator><creator>Whitaker, Darrel</creator><creator>Papadimitriou, John M.</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200001</creationdate><title>Mesothelial regeneration is not dependent on subserosal cells</title><author>Mutsaers, Steven E. ; Whitaker, Darrel ; Papadimitriou, John M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4373-85f277c3020175f101064659e738574e550e85a4300856bd224470e13c87eba93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Autoradiography</topic><topic>Biological and medical sciences</topic><topic>Cell Count</topic><topic>Cell Division</topic><topic>Degeneration. Regeneration. Wound healing. Graft</topic><topic>DNA - biosynthesis</topic><topic>Epithelium - pathology</topic><topic>Epithelium - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Male</topic><topic>mesothelium</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>mouse</topic><topic>precursor</topic><topic>Regeneration - physiology</topic><topic>Testis - physiology</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><topic>wound healing</topic><topic>Wound Healing - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mutsaers, Steven E.</creatorcontrib><creatorcontrib>Whitaker, Darrel</creatorcontrib><creatorcontrib>Papadimitriou, John M.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mutsaers, Steven E.</au><au>Whitaker, Darrel</au><au>Papadimitriou, John M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mesothelial regeneration is not dependent on subserosal cells</atitle><jtitle>The Journal of pathology</jtitle><addtitle>J. Pathol</addtitle><date>2000-01</date><risdate>2000</risdate><volume>190</volume><issue>1</issue><spage>86</spage><epage>92</epage><pages>86-92</pages><issn>0022-3417</issn><eissn>1096-9896</eissn><coden>JPTLAS</coden><abstract>It has been proposed that after mesothelial injury, resident cells within the subserosal connective tissue proliferate, differentiate, and migrate to the serosal surface. The aim of this study was to examine the temporal and spatial changes of proliferating cells in a murine model of testicular mesothelial healing and assess the potential of submesothelial cells to reconstitute the damaged mesothelium. Histology and autoradiography were employed to determine the number of cells within the submesothelial connective tissue, as well as the proportion of cells undergoing DNA synthesis on and beneath the injured serosa. Mesothelial cells surrounding the wound demonstrated maximal DNA synthesis 48 h after injury (27.82±5.64% SEM, compared with 0.17±0.16% 3H‐TdR labelled cells for resting mesothelium), whereas a significant increase in proliferating submesothelial cells was not seen until day 4 post‐injury (7.79±3.31% compared with 0.85±0.64% 3H‐TdR labelled cells at day 2). Furthermore, this small number of dividing submesothelial cells must include cells other than the proposed mesothelial precursors, indicating a very low proportion of precursor cells in the submesothelial cell population. As large numbers of mesothelial cells were seen at the wound centre by 3–4 days after injury, it is unlikely that submesothelial cells contributed significantly to the repopulation of the injured mesothelium. It is hypothesized that regenerating mesothelium is more likely to originate from the surrounding uninjured mesothelial cell population. Copyright © 2000 John Wiley & Sons, Ltd.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>10640997</pmid><doi>10.1002/(SICI)1096-9896(200001)190:1<86::AID-PATH493>3.0.CO;2-G</doi><tpages>7</tpages></addata></record> |
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subjects | Animals Autoradiography Biological and medical sciences Cell Count Cell Division Degeneration. Regeneration. Wound healing. Graft DNA - biosynthesis Epithelium - pathology Epithelium - physiology Fundamental and applied biological sciences. Psychology Male mesothelium Mice Mice, Inbred BALB C mouse precursor Regeneration - physiology Testis - physiology Vertebrates: anatomy and physiology, studies on body, several organs or systems wound healing Wound Healing - physiology |
title | Mesothelial regeneration is not dependent on subserosal cells |
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