Resistance to gentamicin and related aminoglycosides in Staphylococcus aureus isolated in Brazil
Isolates of Staphylococcus aureus obtained from a Brazilian university hospital were characterized in relation to resistance to gentamicin and related aminoglycosides. Thirty‐six isolates were susceptible to methicillin (MSSA) and 14 were resistant (MRSA). All isolates were sensitive to nucleic acid...
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| Veröffentlicht in: | Letters in applied microbiology 1999-09, Vol.29 (3), p.197-201 |
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| creator | Freitas, F. I. S. Guedes‐Stehling, E. Siqueira‐Junior, J. P. |
| description | Isolates of Staphylococcus aureus obtained from a Brazilian university hospital were characterized in relation to resistance to gentamicin and related aminoglycosides. Thirty‐six isolates were susceptible to methicillin (MSSA) and 14 were resistant (MRSA). All isolates were sensitive to nucleic acid‐binding compounds. All MRSA isolates and one MSSA isolate were demonstrated to be resistant to gentamicin and were coincidentally resistant to amikacin, kanamycin, neomycin and tobramycin. Among the gentamicin sensitive MSSA isolates, five isolates were found to be resistant only to kanamycin/neomycin. The resistance to gentamicin (and related aminoglycosides: kanamycin and tobramycin) must be due to AAC(6′)‐APH(2″) activity. As these isolates also showed resistance to neomycin, they must carry an additional genetic element, probably the one responsible for APH(3′)III activity, which accounts for the high level of resistance to kanamycin and to amikacin. The resistance to kanamycin/neomycin in the gentamicin sensitive isolates could not be attributed to the AAD(4′)(4″) activity because of the tobramycin sensitivity, and so could be ascribed to the APH(3′)III activity. Curing and transfer experiments, as well as electrophoresis procedures, indicate that gentamicin resistance in Staph. aureus strains here studied has, characteristically, chromosomal localization. |
| doi_str_mv | 10.1046/j.1365-2672.1999.00617.x |
| format | Article |
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I. S. ; Guedes‐Stehling, E. ; Siqueira‐Junior, J. P.</creator><creatorcontrib>Freitas, F. I. S. ; Guedes‐Stehling, E. ; Siqueira‐Junior, J. P.</creatorcontrib><description>Isolates of Staphylococcus aureus obtained from a Brazilian university hospital were characterized in relation to resistance to gentamicin and related aminoglycosides. Thirty‐six isolates were susceptible to methicillin (MSSA) and 14 were resistant (MRSA). All isolates were sensitive to nucleic acid‐binding compounds. All MRSA isolates and one MSSA isolate were demonstrated to be resistant to gentamicin and were coincidentally resistant to amikacin, kanamycin, neomycin and tobramycin. Among the gentamicin sensitive MSSA isolates, five isolates were found to be resistant only to kanamycin/neomycin. The resistance to gentamicin (and related aminoglycosides: kanamycin and tobramycin) must be due to AAC(6′)‐APH(2″) activity. As these isolates also showed resistance to neomycin, they must carry an additional genetic element, probably the one responsible for APH(3′)III activity, which accounts for the high level of resistance to kanamycin and to amikacin. The resistance to kanamycin/neomycin in the gentamicin sensitive isolates could not be attributed to the AAD(4′)(4″) activity because of the tobramycin sensitivity, and so could be ascribed to the APH(3′)III activity. Curing and transfer experiments, as well as electrophoresis procedures, indicate that gentamicin resistance in Staph. aureus strains here studied has, characteristically, chromosomal localization.</description><identifier>ISSN: 0266-8254</identifier><identifier>EISSN: 1472-765X</identifier><identifier>EISSN: 1365-2673</identifier><identifier>DOI: 10.1046/j.1365-2672.1999.00617.x</identifier><identifier>PMID: 10530041</identifier><identifier>CODEN: LAMIE7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science, Ltd</publisher><subject>Action of physical and chemical agents on bacteria ; Anti-Bacterial Agents - pharmacology ; Bacteriology ; Biological and medical sciences ; Brazil ; Drug Resistance, Microbial - genetics ; Fundamental and applied biological sciences. Psychology ; Gentamicins - pharmacology ; Humans ; Methicillin - pharmacology ; Methicillin Resistance ; Microbial Sensitivity Tests ; Microbiology ; Penicillins - pharmacology ; Staphylococcal Infections - microbiology ; Staphylococcus aureus ; Staphylococcus aureus - drug effects ; Staphylococcus aureus - genetics ; Staphylococcus aureus - isolation & purification</subject><ispartof>Letters in applied microbiology, 1999-09, Vol.29 (3), p.197-201</ispartof><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4907-eeff33d6e0de60da3d1e6706d74db8babd2af11887fddd09399c52bddb7e5d3d3</citedby><cites>FETCH-LOGICAL-c4907-eeff33d6e0de60da3d1e6706d74db8babd2af11887fddd09399c52bddb7e5d3d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2672.1999.00617.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2672.1999.00617.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27915,27916,45565,45566</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1968326$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10530041$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Freitas, F. I. S.</creatorcontrib><creatorcontrib>Guedes‐Stehling, E.</creatorcontrib><creatorcontrib>Siqueira‐Junior, J. P.</creatorcontrib><title>Resistance to gentamicin and related aminoglycosides in Staphylococcus aureus isolated in Brazil</title><title>Letters in applied microbiology</title><addtitle>Lett Appl Microbiol</addtitle><description>Isolates of Staphylococcus aureus obtained from a Brazilian university hospital were characterized in relation to resistance to gentamicin and related aminoglycosides. Thirty‐six isolates were susceptible to methicillin (MSSA) and 14 were resistant (MRSA). All isolates were sensitive to nucleic acid‐binding compounds. All MRSA isolates and one MSSA isolate were demonstrated to be resistant to gentamicin and were coincidentally resistant to amikacin, kanamycin, neomycin and tobramycin. Among the gentamicin sensitive MSSA isolates, five isolates were found to be resistant only to kanamycin/neomycin. The resistance to gentamicin (and related aminoglycosides: kanamycin and tobramycin) must be due to AAC(6′)‐APH(2″) activity. As these isolates also showed resistance to neomycin, they must carry an additional genetic element, probably the one responsible for APH(3′)III activity, which accounts for the high level of resistance to kanamycin and to amikacin. The resistance to kanamycin/neomycin in the gentamicin sensitive isolates could not be attributed to the AAD(4′)(4″) activity because of the tobramycin sensitivity, and so could be ascribed to the APH(3′)III activity. Curing and transfer experiments, as well as electrophoresis procedures, indicate that gentamicin resistance in Staph. aureus strains here studied has, characteristically, chromosomal localization.</description><subject>Action of physical and chemical agents on bacteria</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Brazil</subject><subject>Drug Resistance, Microbial - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gentamicins - pharmacology</subject><subject>Humans</subject><subject>Methicillin - pharmacology</subject><subject>Methicillin Resistance</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbiology</subject><subject>Penicillins - pharmacology</subject><subject>Staphylococcal Infections - microbiology</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Staphylococcus aureus - genetics</subject><subject>Staphylococcus aureus - isolation & purification</subject><issn>0266-8254</issn><issn>1472-765X</issn><issn>1365-2673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU2LFDEQhoMo7rj6F6QP4q3bykcnHfCyLn7BiOAHeIvppHrN0NMZk27c8debsQf0pqcqUs-bgnoIqSg0FIR8tmsol23NpGIN1Vo3AJKq5vYO2VChWK1k--Uu2QCTsu5YKy7Ig5x3ANBRpu-TCwotBxB0Q75-wBzybCeH1RyrG5xmuw8uTJWdfJVwtDP6qjxN8WY8upiDx1yV8cfZHr4dx-iic0uu7JKwlJDjmijEi2R_hvEhuTfYMeOjc70kn1-9_HT9pt6-f_32-mpbO6FB1YjDwLmXCB4leMs9RalAeiV83_W298wOlHadGrz3oLnWrmW9973C1nPPL8nT9d9Dit8XzLPZh-xwHO2EcclGQdcKJvg_QaoEtEqIAnYr6FLMOeFgDinsbToaCuakwezMSYM5aTAnDea3BnNboo_PO5Z-j_6v4Hr3Ajw5AzY7Ow6pCAj5D6dlx5ks2PMV-xFGPP73frO9elca_gvNt6WQ</recordid><startdate>199909</startdate><enddate>199909</enddate><creator>Freitas, F. 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Psychology</topic><topic>Gentamicins - pharmacology</topic><topic>Humans</topic><topic>Methicillin - pharmacology</topic><topic>Methicillin Resistance</topic><topic>Microbial Sensitivity Tests</topic><topic>Microbiology</topic><topic>Penicillins - pharmacology</topic><topic>Staphylococcal Infections - microbiology</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - drug effects</topic><topic>Staphylococcus aureus - genetics</topic><topic>Staphylococcus aureus - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Freitas, F. I. S.</creatorcontrib><creatorcontrib>Guedes‐Stehling, E.</creatorcontrib><creatorcontrib>Siqueira‐Junior, J. 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P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Resistance to gentamicin and related aminoglycosides in Staphylococcus aureus isolated in Brazil</atitle><jtitle>Letters in applied microbiology</jtitle><addtitle>Lett Appl Microbiol</addtitle><date>1999-09</date><risdate>1999</risdate><volume>29</volume><issue>3</issue><spage>197</spage><epage>201</epage><pages>197-201</pages><issn>0266-8254</issn><eissn>1472-765X</eissn><eissn>1365-2673</eissn><coden>LAMIE7</coden><abstract>Isolates of Staphylococcus aureus obtained from a Brazilian university hospital were characterized in relation to resistance to gentamicin and related aminoglycosides. Thirty‐six isolates were susceptible to methicillin (MSSA) and 14 were resistant (MRSA). All isolates were sensitive to nucleic acid‐binding compounds. All MRSA isolates and one MSSA isolate were demonstrated to be resistant to gentamicin and were coincidentally resistant to amikacin, kanamycin, neomycin and tobramycin. Among the gentamicin sensitive MSSA isolates, five isolates were found to be resistant only to kanamycin/neomycin. The resistance to gentamicin (and related aminoglycosides: kanamycin and tobramycin) must be due to AAC(6′)‐APH(2″) activity. As these isolates also showed resistance to neomycin, they must carry an additional genetic element, probably the one responsible for APH(3′)III activity, which accounts for the high level of resistance to kanamycin and to amikacin. The resistance to kanamycin/neomycin in the gentamicin sensitive isolates could not be attributed to the AAD(4′)(4″) activity because of the tobramycin sensitivity, and so could be ascribed to the APH(3′)III activity. Curing and transfer experiments, as well as electrophoresis procedures, indicate that gentamicin resistance in Staph. aureus strains here studied has, characteristically, chromosomal localization.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science, Ltd</pub><pmid>10530041</pmid><doi>10.1046/j.1365-2672.1999.00617.x</doi><tpages>5</tpages></addata></record> |
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| ispartof | Letters in applied microbiology, 1999-09, Vol.29 (3), p.197-201 |
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| language | eng |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | Action of physical and chemical agents on bacteria Anti-Bacterial Agents - pharmacology Bacteriology Biological and medical sciences Brazil Drug Resistance, Microbial - genetics Fundamental and applied biological sciences. Psychology Gentamicins - pharmacology Humans Methicillin - pharmacology Methicillin Resistance Microbial Sensitivity Tests Microbiology Penicillins - pharmacology Staphylococcal Infections - microbiology Staphylococcus aureus Staphylococcus aureus - drug effects Staphylococcus aureus - genetics Staphylococcus aureus - isolation & purification |
| title | Resistance to gentamicin and related aminoglycosides in Staphylococcus aureus isolated in Brazil |
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