Effect of the eluent pH and acidic modifiers in high-performance liquid chromatography retention of basic analytes
The retention of ionogenic bases in liquid chromatography is strongly dependent upon the pH of the mobile phase. Chromatographic behavior of a series of substituted aniline and pyridine basic compounds has been studied on C 18 bonded silica using acetonitrile–water (10:90) as the eluent with differe...
Gespeichert in:
| Veröffentlicht in: | Journal of Chromatography A 2001-04, Vol.913 (1), p.173-187 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 187 |
|---|---|
| container_issue | 1 |
| container_start_page | 173 |
| container_title | Journal of Chromatography A |
| container_volume | 913 |
| creator | LoBrutto, R Jones, A Kazakevich, Y.V McNair, H.M |
| description | The retention of ionogenic bases in liquid chromatography is strongly dependent upon the pH of the mobile phase. Chromatographic behavior of a series of substituted aniline and pyridine basic compounds has been studied on C
18 bonded silica using acetonitrile–water (10:90) as the eluent with different pHs and at various concentrations of the acidic modifier counter anions. The effect of different acidic modifiers on solute retention over a pH range from 1.3 to 8.6 was studied. Ionized basic compounds showed increased retention with a decrease of the mobile phase pH. This increase in retention was attributed to the interaction with counter anions of the acidic modifiers. The increase in retention is dependent on the nature of the counter anion and its concentration in the mobile phase. It was shown that altering the concentration of counter anion of the acidic modifier allows the optimization of the selectivity between basic compounds as well as for neutral and acidic compounds. |
| doi_str_mv | 10.1016/S0021-9673(00)01012-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70851877</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0021967300010128</els_id><sourcerecordid>70851877</sourcerecordid><originalsourceid>FETCH-LOGICAL-c389t-f7a870d0cb636afb29724cc13639bdfd61e4a8866f007b935e00ba3e03ba6a543</originalsourceid><addsrcrecordid>eNqFkcFu1DAQhnMA0VJ4BJAlJASHwDje2M4JoapQpEocgLM1sceNURKndlJp3x5vd9UeOY00-uaf0TdV9YbDJw5cfv4F0PC6k0p8APgIpdfU-ll1_tg-q17m_BeAK1DNi-qMc9G2mvPzKl15T3Zl0bN1IEbjRvPKlmuGs2NogwuWTdEFHyhlFmY2hNuhXij5mCacLbEx3G3BMTukOOEabxMuw54lWktQiPMhucdcYnDGcb9SflU99zhmen2qF9Wfb1e_L6_rm5_ff1x-vamt0N1ae4VagQPbSyHR902nmp21XEjR9c47yWmHWkvpAVTfiZYAehQEokeJ7U5cVO-PuUuKdxvl1UwhWxpHnClu2SjQLddKFbA9gjbFnBN5s6QwYdobDuYg2DwINgeTBsA8CDa6zL09Ldj6idzT1MluAd6dAMwWR5-KsJAfuU42UstCfTlSVGTcF88m20BFrQupvMa4GP5zyD-h1pp9</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>70851877</pqid></control><display><type>article</type><title>Effect of the eluent pH and acidic modifiers in high-performance liquid chromatography retention of basic analytes</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>LoBrutto, R ; Jones, A ; Kazakevich, Y.V ; McNair, H.M</creator><creatorcontrib>LoBrutto, R ; Jones, A ; Kazakevich, Y.V ; McNair, H.M</creatorcontrib><description>The retention of ionogenic bases in liquid chromatography is strongly dependent upon the pH of the mobile phase. Chromatographic behavior of a series of substituted aniline and pyridine basic compounds has been studied on C
18 bonded silica using acetonitrile–water (10:90) as the eluent with different pHs and at various concentrations of the acidic modifier counter anions. The effect of different acidic modifiers on solute retention over a pH range from 1.3 to 8.6 was studied. Ionized basic compounds showed increased retention with a decrease of the mobile phase pH. This increase in retention was attributed to the interaction with counter anions of the acidic modifiers. The increase in retention is dependent on the nature of the counter anion and its concentration in the mobile phase. It was shown that altering the concentration of counter anion of the acidic modifier allows the optimization of the selectivity between basic compounds as well as for neutral and acidic compounds.</description><identifier>ISSN: 0021-9673</identifier><identifier>DOI: 10.1016/S0021-9673(00)01012-8</identifier><identifier>PMID: 11355811</identifier><identifier>CODEN: JOCRAM</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analytical chemistry ; Anilines ; Buffers ; Chemistry ; Chromatographic methods and physical methods associated with chromatography ; Chromatography, High Pressure Liquid - methods ; Exact sciences and technology ; Hydrogen-Ion Concentration ; Other chromatographic methods ; Perchlorates - chemistry ; Phosphates - chemistry ; Phosphoric Acids - chemistry ; Pyridines ; Trichloroacetic Acid - chemistry</subject><ispartof>Journal of Chromatography A, 2001-04, Vol.913 (1), p.173-187</ispartof><rights>2001 Elsevier Science B.V.</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-f7a870d0cb636afb29724cc13639bdfd61e4a8866f007b935e00ba3e03ba6a543</citedby><cites>FETCH-LOGICAL-c389t-f7a870d0cb636afb29724cc13639bdfd61e4a8866f007b935e00ba3e03ba6a543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0021-9673(00)01012-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,780,784,789,790,3550,23930,23931,25140,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=962686$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11355811$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LoBrutto, R</creatorcontrib><creatorcontrib>Jones, A</creatorcontrib><creatorcontrib>Kazakevich, Y.V</creatorcontrib><creatorcontrib>McNair, H.M</creatorcontrib><title>Effect of the eluent pH and acidic modifiers in high-performance liquid chromatography retention of basic analytes</title><title>Journal of Chromatography A</title><addtitle>J Chromatogr A</addtitle><description>The retention of ionogenic bases in liquid chromatography is strongly dependent upon the pH of the mobile phase. Chromatographic behavior of a series of substituted aniline and pyridine basic compounds has been studied on C
18 bonded silica using acetonitrile–water (10:90) as the eluent with different pHs and at various concentrations of the acidic modifier counter anions. The effect of different acidic modifiers on solute retention over a pH range from 1.3 to 8.6 was studied. Ionized basic compounds showed increased retention with a decrease of the mobile phase pH. This increase in retention was attributed to the interaction with counter anions of the acidic modifiers. The increase in retention is dependent on the nature of the counter anion and its concentration in the mobile phase. It was shown that altering the concentration of counter anion of the acidic modifier allows the optimization of the selectivity between basic compounds as well as for neutral and acidic compounds.</description><subject>Analytical chemistry</subject><subject>Anilines</subject><subject>Buffers</subject><subject>Chemistry</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Exact sciences and technology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Other chromatographic methods</subject><subject>Perchlorates - chemistry</subject><subject>Phosphates - chemistry</subject><subject>Phosphoric Acids - chemistry</subject><subject>Pyridines</subject><subject>Trichloroacetic Acid - chemistry</subject><issn>0021-9673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhnMA0VJ4BJAlJASHwDje2M4JoapQpEocgLM1sceNURKndlJp3x5vd9UeOY00-uaf0TdV9YbDJw5cfv4F0PC6k0p8APgIpdfU-ll1_tg-q17m_BeAK1DNi-qMc9G2mvPzKl15T3Zl0bN1IEbjRvPKlmuGs2NogwuWTdEFHyhlFmY2hNuhXij5mCacLbEx3G3BMTukOOEabxMuw54lWktQiPMhucdcYnDGcb9SflU99zhmen2qF9Wfb1e_L6_rm5_ff1x-vamt0N1ae4VagQPbSyHR902nmp21XEjR9c47yWmHWkvpAVTfiZYAehQEokeJ7U5cVO-PuUuKdxvl1UwhWxpHnClu2SjQLddKFbA9gjbFnBN5s6QwYdobDuYg2DwINgeTBsA8CDa6zL09Ldj6idzT1MluAd6dAMwWR5-KsJAfuU42UstCfTlSVGTcF88m20BFrQupvMa4GP5zyD-h1pp9</recordid><startdate>20010413</startdate><enddate>20010413</enddate><creator>LoBrutto, R</creator><creator>Jones, A</creator><creator>Kazakevich, Y.V</creator><creator>McNair, H.M</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010413</creationdate><title>Effect of the eluent pH and acidic modifiers in high-performance liquid chromatography retention of basic analytes</title><author>LoBrutto, R ; Jones, A ; Kazakevich, Y.V ; McNair, H.M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-f7a870d0cb636afb29724cc13639bdfd61e4a8866f007b935e00ba3e03ba6a543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Analytical chemistry</topic><topic>Anilines</topic><topic>Buffers</topic><topic>Chemistry</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Exact sciences and technology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Other chromatographic methods</topic><topic>Perchlorates - chemistry</topic><topic>Phosphates - chemistry</topic><topic>Phosphoric Acids - chemistry</topic><topic>Pyridines</topic><topic>Trichloroacetic Acid - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LoBrutto, R</creatorcontrib><creatorcontrib>Jones, A</creatorcontrib><creatorcontrib>Kazakevich, Y.V</creatorcontrib><creatorcontrib>McNair, H.M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LoBrutto, R</au><au>Jones, A</au><au>Kazakevich, Y.V</au><au>McNair, H.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of the eluent pH and acidic modifiers in high-performance liquid chromatography retention of basic analytes</atitle><jtitle>Journal of Chromatography A</jtitle><addtitle>J Chromatogr A</addtitle><date>2001-04-13</date><risdate>2001</risdate><volume>913</volume><issue>1</issue><spage>173</spage><epage>187</epage><pages>173-187</pages><issn>0021-9673</issn><coden>JOCRAM</coden><abstract>The retention of ionogenic bases in liquid chromatography is strongly dependent upon the pH of the mobile phase. Chromatographic behavior of a series of substituted aniline and pyridine basic compounds has been studied on C
18 bonded silica using acetonitrile–water (10:90) as the eluent with different pHs and at various concentrations of the acidic modifier counter anions. The effect of different acidic modifiers on solute retention over a pH range from 1.3 to 8.6 was studied. Ionized basic compounds showed increased retention with a decrease of the mobile phase pH. This increase in retention was attributed to the interaction with counter anions of the acidic modifiers. The increase in retention is dependent on the nature of the counter anion and its concentration in the mobile phase. It was shown that altering the concentration of counter anion of the acidic modifier allows the optimization of the selectivity between basic compounds as well as for neutral and acidic compounds.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>11355811</pmid><doi>10.1016/S0021-9673(00)01012-8</doi><tpages>15</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9673 |
| ispartof | Journal of Chromatography A, 2001-04, Vol.913 (1), p.173-187 |
| issn | 0021-9673 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70851877 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Analytical chemistry Anilines Buffers Chemistry Chromatographic methods and physical methods associated with chromatography Chromatography, High Pressure Liquid - methods Exact sciences and technology Hydrogen-Ion Concentration Other chromatographic methods Perchlorates - chemistry Phosphates - chemistry Phosphoric Acids - chemistry Pyridines Trichloroacetic Acid - chemistry |
| title | Effect of the eluent pH and acidic modifiers in high-performance liquid chromatography retention of basic analytes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T10%3A44%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20the%20eluent%20pH%20and%20acidic%20modifiers%20in%20high-performance%20liquid%20chromatography%20retention%20of%20basic%20analytes&rft.jtitle=Journal%20of%20Chromatography%20A&rft.au=LoBrutto,%20R&rft.date=2001-04-13&rft.volume=913&rft.issue=1&rft.spage=173&rft.epage=187&rft.pages=173-187&rft.issn=0021-9673&rft.coden=JOCRAM&rft_id=info:doi/10.1016/S0021-9673(00)01012-8&rft_dat=%3Cproquest_cross%3E70851877%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=70851877&rft_id=info:pmid/11355811&rft_els_id=S0021967300010128&rfr_iscdi=true |