In vitro folding and thermodynamic stability of an antibody fragment selected in Vivo for high expression levels in Escherichia coli cytoplasm

We recently isolated a mutant of a human anti-β-galactosidase single chain antibody fragment (scFv) able to fold at high levels in Escherichia coli cytoplasm. When targeted to the periplasm, this mutant and the wild-type scFv are both expressed at comparable levels in a soluble, active and oxidized...

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