Quantitation of Adenovirus DNA and Virus Particles with the PicoGreen Fluorescent Dye

A microplate assay for the rapid quantitation of adenovirus DNA has been developed using the fluorescent dye PicoGreen, which selectively binds double-stranded DNA. The method was first applied to extracted adenoviral DNA and then extended to samples of intact, purified adenovirus after lysis of the...

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Veröffentlicht in:	Analytical biochemistry 1999-10, Vol.274 (2), p.283-288
Hauptverfasser:	Murakami, Pete, McCaman, Michael T.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenoviridae - genetics adenovirus Cell Line DNA quantitation DNA, Single-Stranded - analysis DNA, Viral - analysis Electrophoresis, Polyacrylamide Gel fluorescence particle number Fluorescent Dyes Humans Organic Chemicals PicoGreen RNA - analysis Virion - chemistry
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