Equilibrium Unfolding of Bombyx mori Glycyl-tRNA Synthetase

Unfolding of Bombyx mori glycyl-tRNA synthetase was examined by multiple spectroscopic techniques. Tryptophan fluorescence of wild type enzyme and an N-terminally truncated form (N55) increased at low concentrations of urea or guanidine-HCl followed by a reduction in intensity at intermediate denatu...

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Veröffentlicht in:	The Journal of biological chemistry 2001-02, Vol.276 (6), p.4028-4037
Hauptverfasser:	Dignam, J D, Qu, X, Chaires, J B
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biopolymers Bombyx - enzymology Bombyx mori Circular Dichroism fluorescence Glycine-tRNA Ligase - chemistry Protein Denaturation Spectrometry, Fluorescence tryptophan Tryptophan - chemistry
Online-Zugang:	Volltext
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