Leptin Receptor in Human Term Placenta: in Situ Hybridization and Immunohistochemical Localization
In the present study, we investigated the expression and localization of leptin receptors in human term placentae. On human term placenta tissue slices, digoxigenin-UTP labelled RNA-probe detected the long form of the leptin receptor ObRLmRNA in syncytiotrophoblasts of the villi, whereas the haemato...
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| Veröffentlicht in: | Placenta (Eastbourne) 1999-11, Vol.20 (8), p.677-682 |
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| creator | Bodner, J Ebenbichler, C.F Wolf, H.J Müller-Holzner, E Stanzl, U Gander, R Huter, O Patsch, J.R |
| description | In the present study, we investigated the expression and localization of leptin receptors in human term placentae. On human term placenta tissue slices, digoxigenin-UTP labelled RNA-probe detected the long form of the leptin receptor ObRLmRNA in syncytiotrophoblasts of the villi, whereas the haematological subtype of the leptin receptor ObR/B219.1 was detected in blood cells of the intervillous space and fetal vessels. Immunohistochemistry, with two polyclonal antibodies to the N-terminus recognizing ObRLand ObRSof the leptin receptors and one to the C-terminus recognizing the long form of the leptin receptor ObRL, localized leptin receptor protein at the apical membrane of the syncytiotrophoblasts. Our results show that the long form of the leptin receptor ObRLis expressed in human term placentae. We localized the long form of leptin receptor mRNA to the cytoplasm of syncytiotrophoblasts and leptin receptor proteins in human term placentae to the apical membrane of syncytiotrophoblasts. We conclude that in term placentae, leptin could mediate a growth promoting effect in the fetoplacental unit through the long form of the leptin receptor localized in the syncytiotrophoblasts. In contrast, the haematological subtype of the leptin receptor is not expressed in placental cells, but solely by blood cells in the intervillous space and fetal vessels. |
| doi_str_mv | 10.1053/plac.1999.0431 |
| format | Article |
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On human term placenta tissue slices, digoxigenin-UTP labelled RNA-probe detected the long form of the leptin receptor ObRLmRNA in syncytiotrophoblasts of the villi, whereas the haematological subtype of the leptin receptor ObR/B219.1 was detected in blood cells of the intervillous space and fetal vessels. Immunohistochemistry, with two polyclonal antibodies to the N-terminus recognizing ObRLand ObRSof the leptin receptors and one to the C-terminus recognizing the long form of the leptin receptor ObRL, localized leptin receptor protein at the apical membrane of the syncytiotrophoblasts. Our results show that the long form of the leptin receptor ObRLis expressed in human term placentae. We localized the long form of leptin receptor mRNA to the cytoplasm of syncytiotrophoblasts and leptin receptor proteins in human term placentae to the apical membrane of syncytiotrophoblasts. We conclude that in term placentae, leptin could mediate a growth promoting effect in the fetoplacental unit through the long form of the leptin receptor localized in the syncytiotrophoblasts. In contrast, the haematological subtype of the leptin receptor is not expressed in placental cells, but solely by blood cells in the intervillous space and fetal vessels.</description><identifier>ISSN: 0143-4004</identifier><identifier>EISSN: 1532-3102</identifier><identifier>DOI: 10.1053/plac.1999.0431</identifier><identifier>PMID: 10527822</identifier><identifier>CODEN: PLACDF</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Biological and medical sciences ; Carrier Proteins - analysis ; Cesarean Section ; Female ; Fundamental and applied biological sciences. 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On human term placenta tissue slices, digoxigenin-UTP labelled RNA-probe detected the long form of the leptin receptor ObRLmRNA in syncytiotrophoblasts of the villi, whereas the haematological subtype of the leptin receptor ObR/B219.1 was detected in blood cells of the intervillous space and fetal vessels. Immunohistochemistry, with two polyclonal antibodies to the N-terminus recognizing ObRLand ObRSof the leptin receptors and one to the C-terminus recognizing the long form of the leptin receptor ObRL, localized leptin receptor protein at the apical membrane of the syncytiotrophoblasts. Our results show that the long form of the leptin receptor ObRLis expressed in human term placentae. We localized the long form of leptin receptor mRNA to the cytoplasm of syncytiotrophoblasts and leptin receptor proteins in human term placentae to the apical membrane of syncytiotrophoblasts. We conclude that in term placentae, leptin could mediate a growth promoting effect in the fetoplacental unit through the long form of the leptin receptor localized in the syncytiotrophoblasts. In contrast, the haematological subtype of the leptin receptor is not expressed in placental cells, but solely by blood cells in the intervillous space and fetal vessels.</description><subject>Biological and medical sciences</subject><subject>Carrier Proteins - analysis</subject><subject>Cesarean Section</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>In Situ Hybridization</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Placenta - chemistry</subject><subject>Pregnancy</subject><subject>Receptors, Cell Surface</subject><subject>Receptors, Cytokine - analysis</subject><subject>Receptors, Leptin</subject><subject>Signal Transduction - physiology</subject><issn>0143-4004</issn><issn>1532-3102</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM9LwzAUx4Mobk6vHqUH8daZX11bbzLUDQqKznNI01cWaZOZtML8603pQC9ekkf4fN_L-yB0SfCc4ITd7hqp5iTP8znmjByhKUkYjRnB9BhNMeEs5hjzCTrz_gNjnHNCT9EkRGmaUTpFZQG7TpvoFVQorItCvepbaaINuDZ6Ce3BdPJueH_TXR-t9qXTlf6WnbYmkqaK1m3bG7vVvrNqC61WsokKG84DdI5Oatl4uDjcM_T--LBZruLi-Wm9vC9ixVnSxQsiK8IkKLngWUV4npZMQcUxTUqVJglmkqUkSxPCec6AZYoCIXlWcVWymjM2Qzdj352znz34TrTaK2gaacD2XqQ44zQJ2Rmaj6By1nsHtdg53Uq3FwSLwaoYrIrBqhishsDVoXNftlD9wUeNAbg-ANKHxWsnjdL-l6MsDF4ELBsxCBq-NDjhlQYTttQOVCcqq__7wg8-H5Ma</recordid><startdate>19991101</startdate><enddate>19991101</enddate><creator>Bodner, J</creator><creator>Ebenbichler, C.F</creator><creator>Wolf, H.J</creator><creator>Müller-Holzner, E</creator><creator>Stanzl, U</creator><creator>Gander, R</creator><creator>Huter, O</creator><creator>Patsch, J.R</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19991101</creationdate><title>Leptin Receptor in Human Term Placenta: in Situ Hybridization and Immunohistochemical Localization</title><author>Bodner, J ; Ebenbichler, C.F ; Wolf, H.J ; Müller-Holzner, E ; Stanzl, U ; Gander, R ; Huter, O ; Patsch, J.R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c435t-61ad13aeca648d1497b3ced4025bc75503a37187514493e38c2e1198d4cb3f433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Biological and medical sciences</topic><topic>Carrier Proteins - analysis</topic><topic>Cesarean Section</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>In Situ Hybridization</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Placenta - chemistry</topic><topic>Pregnancy</topic><topic>Receptors, Cell Surface</topic><topic>Receptors, Cytokine - analysis</topic><topic>Receptors, Leptin</topic><topic>Signal Transduction - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bodner, J</creatorcontrib><creatorcontrib>Ebenbichler, C.F</creatorcontrib><creatorcontrib>Wolf, H.J</creatorcontrib><creatorcontrib>Müller-Holzner, E</creatorcontrib><creatorcontrib>Stanzl, U</creatorcontrib><creatorcontrib>Gander, R</creatorcontrib><creatorcontrib>Huter, O</creatorcontrib><creatorcontrib>Patsch, J.R</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Placenta (Eastbourne)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bodner, J</au><au>Ebenbichler, C.F</au><au>Wolf, H.J</au><au>Müller-Holzner, E</au><au>Stanzl, U</au><au>Gander, R</au><au>Huter, O</au><au>Patsch, J.R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Leptin Receptor in Human Term Placenta: in Situ Hybridization and Immunohistochemical Localization</atitle><jtitle>Placenta (Eastbourne)</jtitle><addtitle>Placenta</addtitle><date>1999-11-01</date><risdate>1999</risdate><volume>20</volume><issue>8</issue><spage>677</spage><epage>682</epage><pages>677-682</pages><issn>0143-4004</issn><eissn>1532-3102</eissn><coden>PLACDF</coden><abstract>In the present study, we investigated the expression and localization of leptin receptors in human term placentae. On human term placenta tissue slices, digoxigenin-UTP labelled RNA-probe detected the long form of the leptin receptor ObRLmRNA in syncytiotrophoblasts of the villi, whereas the haematological subtype of the leptin receptor ObR/B219.1 was detected in blood cells of the intervillous space and fetal vessels. Immunohistochemistry, with two polyclonal antibodies to the N-terminus recognizing ObRLand ObRSof the leptin receptors and one to the C-terminus recognizing the long form of the leptin receptor ObRL, localized leptin receptor protein at the apical membrane of the syncytiotrophoblasts. Our results show that the long form of the leptin receptor ObRLis expressed in human term placentae. We localized the long form of leptin receptor mRNA to the cytoplasm of syncytiotrophoblasts and leptin receptor proteins in human term placentae to the apical membrane of syncytiotrophoblasts. We conclude that in term placentae, leptin could mediate a growth promoting effect in the fetoplacental unit through the long form of the leptin receptor localized in the syncytiotrophoblasts. In contrast, the haematological subtype of the leptin receptor is not expressed in placental cells, but solely by blood cells in the intervillous space and fetal vessels.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>10527822</pmid><doi>10.1053/plac.1999.0431</doi><tpages>6</tpages></addata></record> |
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| subjects | Biological and medical sciences Carrier Proteins - analysis Cesarean Section Female Fundamental and applied biological sciences. Psychology Gene expression Humans Immunohistochemistry In Situ Hybridization Molecular and cellular biology Molecular genetics Placenta - chemistry Pregnancy Receptors, Cell Surface Receptors, Cytokine - analysis Receptors, Leptin Signal Transduction - physiology |
| title | Leptin Receptor in Human Term Placenta: in Situ Hybridization and Immunohistochemical Localization |
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