Immunohistochemical analysis of NY‐ESO‐1 antigen expression in normal and malignant human tissues
NY‐ESO‐1, a member of the CT (cancer/testis) family of antigens, is expressed in normal testis and in a range of human tumor types. Knowledge of NY‐ESO‐1 expression has depended on RT‐PCR detection of mRNA and there is a need for detecting NY‐ESO‐1 at the protein level. In the present study, a metho...
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| Veröffentlicht in: | International journal of cancer 2001-06, Vol.92 (6), p.856-860 |
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| container_title | International journal of cancer |
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| creator | Jungbluth, Achim A. Chen, Yao‐Tseng Stockert, Elisabeth Busam, Klaus J. Kolb, Denise Iversen, Kristin Coplan, Keren Williamson, Barbara Altorki, Nasser Old, Lloyd J. |
| description | NY‐ESO‐1, a member of the CT (cancer/testis) family of antigens, is expressed in normal testis and in a range of human tumor types. Knowledge of NY‐ESO‐1 expression has depended on RT‐PCR detection of mRNA and there is a need for detecting NY‐ESO‐1 at the protein level. In the present study, a method for the immunochemical detection of NY‐ESO‐1 in paraffin‐embedded tissues has been developed and used to define the expression pattern of NY‐ESO‐1 in normal tissues and in a panel of human tumors. No normal tissue other than testis showed NY‐ESO‐1 reactivity, and expression in testis was restricted to germ cells particularly spermatogonia. In human tumors, the frequency of NY‐ESO‐1 antigen expression corresponds with past analysis of NY‐ESO‐1 mRNA expression e.g., 20–30% of lung cancers, bladder cancers and melanoma, and no expression in colon and renal cancer. Co‐typing of NY‐ESO‐1 antigen and mRNA expression in a large panel of lung cancers showed a good correlation. There is great variability in NY‐ESO‐1 expression in individual tumors, ranging from an infrequent homogeneous pattern of staining to highly heterogeneous antigen expression. © 2001 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/ijc.1282 |
| format | Article |
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Knowledge of NY‐ESO‐1 expression has depended on RT‐PCR detection of mRNA and there is a need for detecting NY‐ESO‐1 at the protein level. In the present study, a method for the immunochemical detection of NY‐ESO‐1 in paraffin‐embedded tissues has been developed and used to define the expression pattern of NY‐ESO‐1 in normal tissues and in a panel of human tumors. No normal tissue other than testis showed NY‐ESO‐1 reactivity, and expression in testis was restricted to germ cells particularly spermatogonia. In human tumors, the frequency of NY‐ESO‐1 antigen expression corresponds with past analysis of NY‐ESO‐1 mRNA expression e.g., 20–30% of lung cancers, bladder cancers and melanoma, and no expression in colon and renal cancer. Co‐typing of NY‐ESO‐1 antigen and mRNA expression in a large panel of lung cancers showed a good correlation. There is great variability in NY‐ESO‐1 expression in individual tumors, ranging from an infrequent homogeneous pattern of staining to highly heterogeneous antigen expression. © 2001 Wiley‐Liss, Inc.</description><identifier>ISSN: 0020-7136</identifier><identifier>EISSN: 1097-0215</identifier><identifier>DOI: 10.1002/ijc.1282</identifier><identifier>PMID: 11351307</identifier><identifier>CODEN: IJCNAW</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Antibodies, Monoclonal - metabolism ; antigen expression ; Antigens, Neoplasm ; Biological and medical sciences ; Breast Neoplasms - metabolism ; Carcinoma, Renal Cell - metabolism ; Colonic Neoplasms - metabolism ; CT‐antigen ; ES121 ; Female ; Head and Neck Neoplasms - metabolism ; Host-tumor relations. Immunology. Biological markers ; Humans ; Immunohistochemistry ; Kidney Neoplasms - metabolism ; Lung Neoplasms - metabolism ; Male ; Medical sciences ; Melanoma - metabolism ; Membrane Proteins ; NY‐ESO‐1 ; Protein Biosynthesis ; Reverse Transcriptase Polymerase Chain Reaction ; Spermatogonia - metabolism ; Testis - metabolism ; Tissue Distribution ; Tumor Cells, Cultured ; Tumors ; tumor‐associated antigen ; Urinary Bladder Neoplasms - metabolism</subject><ispartof>International journal of cancer, 2001-06, Vol.92 (6), p.856-860</ispartof><rights>Copyright © 2001 Wiley‐Liss, Inc.</rights><rights>2001 INIST-CNRS</rights><rights>Copyright 2001 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4122-69f2c0b61259201f10801395b589c399ec1121ac79754b6f6c8a727a856432033</citedby><cites>FETCH-LOGICAL-c4122-69f2c0b61259201f10801395b589c399ec1121ac79754b6f6c8a727a856432033</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fijc.1282$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fijc.1282$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1088887$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11351307$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jungbluth, Achim A.</creatorcontrib><creatorcontrib>Chen, Yao‐Tseng</creatorcontrib><creatorcontrib>Stockert, Elisabeth</creatorcontrib><creatorcontrib>Busam, Klaus J.</creatorcontrib><creatorcontrib>Kolb, Denise</creatorcontrib><creatorcontrib>Iversen, Kristin</creatorcontrib><creatorcontrib>Coplan, Keren</creatorcontrib><creatorcontrib>Williamson, Barbara</creatorcontrib><creatorcontrib>Altorki, Nasser</creatorcontrib><creatorcontrib>Old, Lloyd J.</creatorcontrib><title>Immunohistochemical analysis of NY‐ESO‐1 antigen expression in normal and malignant human tissues</title><title>International journal of cancer</title><addtitle>Int J Cancer</addtitle><description>NY‐ESO‐1, a member of the CT (cancer/testis) family of antigens, is expressed in normal testis and in a range of human tumor types. Knowledge of NY‐ESO‐1 expression has depended on RT‐PCR detection of mRNA and there is a need for detecting NY‐ESO‐1 at the protein level. In the present study, a method for the immunochemical detection of NY‐ESO‐1 in paraffin‐embedded tissues has been developed and used to define the expression pattern of NY‐ESO‐1 in normal tissues and in a panel of human tumors. No normal tissue other than testis showed NY‐ESO‐1 reactivity, and expression in testis was restricted to germ cells particularly spermatogonia. In human tumors, the frequency of NY‐ESO‐1 antigen expression corresponds with past analysis of NY‐ESO‐1 mRNA expression e.g., 20–30% of lung cancers, bladder cancers and melanoma, and no expression in colon and renal cancer. Co‐typing of NY‐ESO‐1 antigen and mRNA expression in a large panel of lung cancers showed a good correlation. There is great variability in NY‐ESO‐1 expression in individual tumors, ranging from an infrequent homogeneous pattern of staining to highly heterogeneous antigen expression. © 2001 Wiley‐Liss, Inc.</description><subject>Antibodies, Monoclonal - metabolism</subject><subject>antigen expression</subject><subject>Antigens, Neoplasm</subject><subject>Biological and medical sciences</subject><subject>Breast Neoplasms - metabolism</subject><subject>Carcinoma, Renal Cell - metabolism</subject><subject>Colonic Neoplasms - metabolism</subject><subject>CT‐antigen</subject><subject>ES121</subject><subject>Female</subject><subject>Head and Neck Neoplasms - metabolism</subject><subject>Host-tumor relations. Immunology. Biological markers</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Kidney Neoplasms - metabolism</subject><subject>Lung Neoplasms - metabolism</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Melanoma - metabolism</subject><subject>Membrane Proteins</subject><subject>NY‐ESO‐1</subject><subject>Protein Biosynthesis</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Spermatogonia - metabolism</subject><subject>Testis - metabolism</subject><subject>Tissue Distribution</subject><subject>Tumor Cells, Cultured</subject><subject>Tumors</subject><subject>tumor‐associated antigen</subject><subject>Urinary Bladder Neoplasms - metabolism</subject><issn>0020-7136</issn><issn>1097-0215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kLtOAzEQRS0EgvCQ-ALkAiGahRl7Xy5RFCAIkQIoqFaO8RKjXW_YyQrS8Ql8I1-C85CgwcVMMcf3SoexQ4QzBBDn7tWcocjFBushqCwCgckm64UTRBnKdIftEr0CICYQb7MdRJmghKzH7LCuO99MHM0aM7G1M7ri2utqTo54U_K7p-_Pr8H9KEwMh5l7sZ7bj2lriVzjufPcN229_PXMw3YvPmB80tXa85kj6izts61SV2QP1nuPPV4OHvrX0e3oati_uI1MjEJEqSqFgXGKIlECsETIAaVKxkmujFTKGkSB2mQqS-JxWqYm15nIdJ6ksRQg5R47WeVO2-Yt9M6K2pGxVaW9bToqMshjIaUK4OkKNG1D1NqymLau1u28QCgWSougtFgoDejROrMb1_b5F1w7DMDxGtAU7JWt9sbRn8A8vAUWrbB3V9n5v33F8Ka_7P0BdMKM7Q</recordid><startdate>20010615</startdate><enddate>20010615</enddate><creator>Jungbluth, Achim A.</creator><creator>Chen, Yao‐Tseng</creator><creator>Stockert, Elisabeth</creator><creator>Busam, Klaus J.</creator><creator>Kolb, Denise</creator><creator>Iversen, Kristin</creator><creator>Coplan, Keren</creator><creator>Williamson, Barbara</creator><creator>Altorki, Nasser</creator><creator>Old, Lloyd J.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010615</creationdate><title>Immunohistochemical analysis of NY‐ESO‐1 antigen expression in normal and malignant human tissues</title><author>Jungbluth, Achim A. ; Chen, Yao‐Tseng ; Stockert, Elisabeth ; Busam, Klaus J. ; Kolb, Denise ; Iversen, Kristin ; Coplan, Keren ; Williamson, Barbara ; Altorki, Nasser ; Old, Lloyd J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4122-69f2c0b61259201f10801395b589c399ec1121ac79754b6f6c8a727a856432033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Antibodies, Monoclonal - metabolism</topic><topic>antigen expression</topic><topic>Antigens, Neoplasm</topic><topic>Biological and medical sciences</topic><topic>Breast Neoplasms - metabolism</topic><topic>Carcinoma, Renal Cell - metabolism</topic><topic>Colonic Neoplasms - metabolism</topic><topic>CT‐antigen</topic><topic>ES121</topic><topic>Female</topic><topic>Head and Neck Neoplasms - metabolism</topic><topic>Host-tumor relations. Immunology. Biological markers</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Kidney Neoplasms - metabolism</topic><topic>Lung Neoplasms - metabolism</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Melanoma - metabolism</topic><topic>Membrane Proteins</topic><topic>NY‐ESO‐1</topic><topic>Protein Biosynthesis</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Spermatogonia - metabolism</topic><topic>Testis - metabolism</topic><topic>Tissue Distribution</topic><topic>Tumor Cells, Cultured</topic><topic>Tumors</topic><topic>tumor‐associated antigen</topic><topic>Urinary Bladder Neoplasms - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jungbluth, Achim A.</creatorcontrib><creatorcontrib>Chen, Yao‐Tseng</creatorcontrib><creatorcontrib>Stockert, Elisabeth</creatorcontrib><creatorcontrib>Busam, Klaus J.</creatorcontrib><creatorcontrib>Kolb, Denise</creatorcontrib><creatorcontrib>Iversen, Kristin</creatorcontrib><creatorcontrib>Coplan, Keren</creatorcontrib><creatorcontrib>Williamson, Barbara</creatorcontrib><creatorcontrib>Altorki, Nasser</creatorcontrib><creatorcontrib>Old, Lloyd J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jungbluth, Achim A.</au><au>Chen, Yao‐Tseng</au><au>Stockert, Elisabeth</au><au>Busam, Klaus J.</au><au>Kolb, Denise</au><au>Iversen, Kristin</au><au>Coplan, Keren</au><au>Williamson, Barbara</au><au>Altorki, Nasser</au><au>Old, Lloyd J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunohistochemical analysis of NY‐ESO‐1 antigen expression in normal and malignant human tissues</atitle><jtitle>International journal of cancer</jtitle><addtitle>Int J Cancer</addtitle><date>2001-06-15</date><risdate>2001</risdate><volume>92</volume><issue>6</issue><spage>856</spage><epage>860</epage><pages>856-860</pages><issn>0020-7136</issn><eissn>1097-0215</eissn><coden>IJCNAW</coden><abstract>NY‐ESO‐1, a member of the CT (cancer/testis) family of antigens, is expressed in normal testis and in a range of human tumor types. Knowledge of NY‐ESO‐1 expression has depended on RT‐PCR detection of mRNA and there is a need for detecting NY‐ESO‐1 at the protein level. In the present study, a method for the immunochemical detection of NY‐ESO‐1 in paraffin‐embedded tissues has been developed and used to define the expression pattern of NY‐ESO‐1 in normal tissues and in a panel of human tumors. No normal tissue other than testis showed NY‐ESO‐1 reactivity, and expression in testis was restricted to germ cells particularly spermatogonia. In human tumors, the frequency of NY‐ESO‐1 antigen expression corresponds with past analysis of NY‐ESO‐1 mRNA expression e.g., 20–30% of lung cancers, bladder cancers and melanoma, and no expression in colon and renal cancer. Co‐typing of NY‐ESO‐1 antigen and mRNA expression in a large panel of lung cancers showed a good correlation. There is great variability in NY‐ESO‐1 expression in individual tumors, ranging from an infrequent homogeneous pattern of staining to highly heterogeneous antigen expression. © 2001 Wiley‐Liss, Inc.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>11351307</pmid><doi>10.1002/ijc.1282</doi><tpages>5</tpages></addata></record> |
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| subjects | Antibodies, Monoclonal - metabolism antigen expression Antigens, Neoplasm Biological and medical sciences Breast Neoplasms - metabolism Carcinoma, Renal Cell - metabolism Colonic Neoplasms - metabolism CT‐antigen ES121 Female Head and Neck Neoplasms - metabolism Host-tumor relations. Immunology. Biological markers Humans Immunohistochemistry Kidney Neoplasms - metabolism Lung Neoplasms - metabolism Male Medical sciences Melanoma - metabolism Membrane Proteins NY‐ESO‐1 Protein Biosynthesis Reverse Transcriptase Polymerase Chain Reaction Spermatogonia - metabolism Testis - metabolism Tissue Distribution Tumor Cells, Cultured Tumors tumor‐associated antigen Urinary Bladder Neoplasms - metabolism |
| title | Immunohistochemical analysis of NY‐ESO‐1 antigen expression in normal and malignant human tissues |
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