A Closed-Cycle Capillary Polymerase Chain Reaction Machine
A novel thermocycling machine based on a microcapillary equipped with bidirectional pressure-driven flow and in situ optical position sensors is described. A 1-μL droplet of reaction mixture moves between three heat zones in a 1-mm-i.d., oil-filled capillary using a multielement scattered light dete...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2001-05, Vol.73 (9), p.2018-2021 |
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| container_end_page | 2021 |
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| container_issue | 9 |
| container_start_page | 2018 |
| container_title | Analytical chemistry (Washington) |
| container_volume | 73 |
| creator | Chiou, Jeff Matsudaira, Paul Sonin, Ain Ehrlich, Daniel |
| description | A novel thermocycling machine based on a microcapillary equipped with bidirectional pressure-driven flow and in situ optical position sensors is described. A 1-μL droplet of reaction mixture moves between three heat zones in a 1-mm-i.d., oil-filled capillary using a multielement scattered light detector and active feedback. Dwell times and accelerations can be adjusted independently. As a demonstration of the device, 30 cycles of a 500-base pair product were performed in 23 min with 78% amplification efficiency. This result compares well with previous high-speed thermocyclers. Theoretically, the arrangement can approach a time of 2.5 min for 30 cycle amplifications of a 500-base pair product. |
| doi_str_mv | 10.1021/ac001227f |
| format | Article |
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A 1-μL droplet of reaction mixture moves between three heat zones in a 1-mm-i.d., oil-filled capillary using a multielement scattered light detector and active feedback. Dwell times and accelerations can be adjusted independently. As a demonstration of the device, 30 cycles of a 500-base pair product were performed in 23 min with 78% amplification efficiency. This result compares well with previous high-speed thermocyclers. Theoretically, the arrangement can approach a time of 2.5 min for 30 cycle amplifications of a 500-base pair product.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac001227f</identifier><identifier>PMID: 11354484</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Biological and medical sciences ; Chemistry ; Diverse techniques ; DNA - analysis ; Fundamental and applied biological sciences. 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| language | eng |
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| source | MEDLINE; American Chemical Society Journals |
| subjects | Biological and medical sciences Chemistry Diverse techniques DNA - analysis Fundamental and applied biological sciences. Psychology Hot Temperature Instruments Molecular and cellular biology Optics Polymerase Chain Reaction - instrumentation Polymerase Chain Reaction - standards Sensors Time |
| title | A Closed-Cycle Capillary Polymerase Chain Reaction Machine |
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