Molecular cytogenetic resources specific for chromosome 12
We have generated a panel of 20 somatic cell hybrids retaining fragments of human chromosome 12. Each hybrid was characterized cytogenetically by reverse fluorescence in situ hybridization (FISH) and molecularly by 24 sequence tagged sites (STSs) spaced evenly along the chromosome. The panel can be...
Gespeichert in:
| Veröffentlicht in: | American journal of medical genetics 1999-11, Vol.87 (1), p.40-44 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 44 |
|---|---|
| container_issue | 1 |
| container_start_page | 40 |
| container_title | American journal of medical genetics |
| container_volume | 87 |
| creator | Viggiano, Luigi Miolla, Valeria Ricco, Angela Storlazzi, Clelia Tiziana Pennacchia, Margherita Fratello, Angelo Mancini, Mita Marzella, Rosalia Archidiacono, Nicoletta Rocchi, Mariano |
| description | We have generated a panel of 20 somatic cell hybrids retaining fragments of human chromosome 12. Each hybrid was characterized cytogenetically by reverse fluorescence in situ hybridization (FISH) and molecularly by 24 sequence tagged sites (STSs) spaced evenly along the chromosome. The panel can be exploited to map subregionally DNA sequences on chromosome 12 and to generate partial chromosome paints useful in the characterization of chromosomal rearrangements involving this chromosome. Furthermore, a panel of 58 yeast artificial chromosomes (YACs) mapping to chromosome 12 was characterized by FISH experiments on normal human metaphases. A subset of this panel is recognized by the STSs used in the somatic cell hybrid characterization. In this way a correlation between the genetic and the physical maps of this chromosome can be established. Am. J. Med. Genet. 87:40–44, 1999. © 1999 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/(SICI)1096-8628(19991105)87:1<40::AID-AJMG8>3.0.CO;2-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70841743</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>70841743</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3488-ade0e68fbbe8d91ab180bb5bec615f58637f9506c73c60c6147322df2a9dd47a3</originalsourceid><addsrcrecordid>eNqFkF1v0zAUhi0EYmXwF1AvENouUo4_EjsFTaoClKJtlRjQyyPHcSAjqYvdaOu_xyVlIIHElaVXz3n1-iHkjMKEArAXJ1eLYnFKIc8SlTF1QvM8pxTSUyWn9JWA6XS2eJ3M3l_M1RmfwKRYvmQJu0dGdyf3yQioUIlkeX5EHoVwDUBjwB6So1jEFBPpiEwvXGtN32o_Nrut-2LXdtuYsbfB9d7YMA4ba5o6RrWLyFfvOhdcZ8eUPSYPat0G--TwHpNPb998LN4l58v5opidJ4YLpRJdWbCZqsvSqiqnuqQKyjItrcloWqcq47LOU8iM5CaDGArJGatqpvOqElLzY_J86N149723YYtdE4xtW722rg8oQQkqBY_g5wE03oXgbY0b33Ta75AC7q0i7q3iXhHuFeEvq6gkUhSAGK3iT6vIEbBYIkMWi58eFvRlZ6s_ageNEXh2AHQwuq29Xpsm_OaoYvFPEVsN2E3T2t1f6_437l_bhiA2J0NzE7b29q5Z-2-YSS5TXF3OUaWrD_RKXCLwH6D4rdk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>70841743</pqid></control><display><type>article</type><title>Molecular cytogenetic resources specific for chromosome 12</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Viggiano, Luigi ; Miolla, Valeria ; Ricco, Angela ; Storlazzi, Clelia Tiziana ; Pennacchia, Margherita ; Fratello, Angelo ; Mancini, Mita ; Marzella, Rosalia ; Archidiacono, Nicoletta ; Rocchi, Mariano</creator><creatorcontrib>Viggiano, Luigi ; Miolla, Valeria ; Ricco, Angela ; Storlazzi, Clelia Tiziana ; Pennacchia, Margherita ; Fratello, Angelo ; Mancini, Mita ; Marzella, Rosalia ; Archidiacono, Nicoletta ; Rocchi, Mariano</creatorcontrib><description>We have generated a panel of 20 somatic cell hybrids retaining fragments of human chromosome 12. Each hybrid was characterized cytogenetically by reverse fluorescence in situ hybridization (FISH) and molecularly by 24 sequence tagged sites (STSs) spaced evenly along the chromosome. The panel can be exploited to map subregionally DNA sequences on chromosome 12 and to generate partial chromosome paints useful in the characterization of chromosomal rearrangements involving this chromosome. Furthermore, a panel of 58 yeast artificial chromosomes (YACs) mapping to chromosome 12 was characterized by FISH experiments on normal human metaphases. A subset of this panel is recognized by the STSs used in the somatic cell hybrid characterization. In this way a correlation between the genetic and the physical maps of this chromosome can be established. Am. J. Med. Genet. 87:40–44, 1999. © 1999 Wiley‐Liss, Inc.</description><identifier>ISSN: 0148-7299</identifier><identifier>EISSN: 1096-8628</identifier><identifier>DOI: 10.1002/(SICI)1096-8628(19991105)87:1<40::AID-AJMG8>3.0.CO;2-2</identifier><identifier>PMID: 10528245</identifier><identifier>CODEN: AJMGDA</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Biological and medical sciences ; chromosome 12 ; Chromosome Mapping ; Chromosomes, Artificial, Yeast ; Chromosomes, Human, Pair 12 - genetics ; Cricetinae ; Cytogenetic Analysis ; General aspects. Genetic counseling ; Humans ; Hybrid Cells ; In Situ Hybridization, Fluorescence ; Medical genetics ; Medical sciences ; radiation hybrids ; Sequence Tagged Sites ; YACs</subject><ispartof>American journal of medical genetics, 1999-11, Vol.87 (1), p.40-44</ispartof><rights>Copyright © 1999 Wiley‐Liss, Inc.</rights><rights>2000 INIST-CNRS</rights><rights>Copyright 1999 Wiley-Liss, Inc.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3488-ade0e68fbbe8d91ab180bb5bec615f58637f9506c73c60c6147322df2a9dd47a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1182322$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10528245$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Viggiano, Luigi</creatorcontrib><creatorcontrib>Miolla, Valeria</creatorcontrib><creatorcontrib>Ricco, Angela</creatorcontrib><creatorcontrib>Storlazzi, Clelia Tiziana</creatorcontrib><creatorcontrib>Pennacchia, Margherita</creatorcontrib><creatorcontrib>Fratello, Angelo</creatorcontrib><creatorcontrib>Mancini, Mita</creatorcontrib><creatorcontrib>Marzella, Rosalia</creatorcontrib><creatorcontrib>Archidiacono, Nicoletta</creatorcontrib><creatorcontrib>Rocchi, Mariano</creatorcontrib><title>Molecular cytogenetic resources specific for chromosome 12</title><title>American journal of medical genetics</title><addtitle>Am. J. Med. Genet</addtitle><description>We have generated a panel of 20 somatic cell hybrids retaining fragments of human chromosome 12. Each hybrid was characterized cytogenetically by reverse fluorescence in situ hybridization (FISH) and molecularly by 24 sequence tagged sites (STSs) spaced evenly along the chromosome. The panel can be exploited to map subregionally DNA sequences on chromosome 12 and to generate partial chromosome paints useful in the characterization of chromosomal rearrangements involving this chromosome. Furthermore, a panel of 58 yeast artificial chromosomes (YACs) mapping to chromosome 12 was characterized by FISH experiments on normal human metaphases. A subset of this panel is recognized by the STSs used in the somatic cell hybrid characterization. In this way a correlation between the genetic and the physical maps of this chromosome can be established. Am. J. Med. Genet. 87:40–44, 1999. © 1999 Wiley‐Liss, Inc.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>chromosome 12</subject><subject>Chromosome Mapping</subject><subject>Chromosomes, Artificial, Yeast</subject><subject>Chromosomes, Human, Pair 12 - genetics</subject><subject>Cricetinae</subject><subject>Cytogenetic Analysis</subject><subject>General aspects. Genetic counseling</subject><subject>Humans</subject><subject>Hybrid Cells</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Medical genetics</subject><subject>Medical sciences</subject><subject>radiation hybrids</subject><subject>Sequence Tagged Sites</subject><subject>YACs</subject><issn>0148-7299</issn><issn>1096-8628</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkF1v0zAUhi0EYmXwF1AvENouUo4_EjsFTaoClKJtlRjQyyPHcSAjqYvdaOu_xyVlIIHElaVXz3n1-iHkjMKEArAXJ1eLYnFKIc8SlTF1QvM8pxTSUyWn9JWA6XS2eJ3M3l_M1RmfwKRYvmQJu0dGdyf3yQioUIlkeX5EHoVwDUBjwB6So1jEFBPpiEwvXGtN32o_Nrut-2LXdtuYsbfB9d7YMA4ba5o6RrWLyFfvOhdcZ8eUPSYPat0G--TwHpNPb998LN4l58v5opidJ4YLpRJdWbCZqsvSqiqnuqQKyjItrcloWqcq47LOU8iM5CaDGArJGatqpvOqElLzY_J86N149723YYtdE4xtW722rg8oQQkqBY_g5wE03oXgbY0b33Ta75AC7q0i7q3iXhHuFeEvq6gkUhSAGK3iT6vIEbBYIkMWi58eFvRlZ6s_ageNEXh2AHQwuq29Xpsm_OaoYvFPEVsN2E3T2t1f6_437l_bhiA2J0NzE7b29q5Z-2-YSS5TXF3OUaWrD_RKXCLwH6D4rdk</recordid><startdate>19991105</startdate><enddate>19991105</enddate><creator>Viggiano, Luigi</creator><creator>Miolla, Valeria</creator><creator>Ricco, Angela</creator><creator>Storlazzi, Clelia Tiziana</creator><creator>Pennacchia, Margherita</creator><creator>Fratello, Angelo</creator><creator>Mancini, Mita</creator><creator>Marzella, Rosalia</creator><creator>Archidiacono, Nicoletta</creator><creator>Rocchi, Mariano</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19991105</creationdate><title>Molecular cytogenetic resources specific for chromosome 12</title><author>Viggiano, Luigi ; Miolla, Valeria ; Ricco, Angela ; Storlazzi, Clelia Tiziana ; Pennacchia, Margherita ; Fratello, Angelo ; Mancini, Mita ; Marzella, Rosalia ; Archidiacono, Nicoletta ; Rocchi, Mariano</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3488-ade0e68fbbe8d91ab180bb5bec615f58637f9506c73c60c6147322df2a9dd47a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>chromosome 12</topic><topic>Chromosome Mapping</topic><topic>Chromosomes, Artificial, Yeast</topic><topic>Chromosomes, Human, Pair 12 - genetics</topic><topic>Cricetinae</topic><topic>Cytogenetic Analysis</topic><topic>General aspects. Genetic counseling</topic><topic>Humans</topic><topic>Hybrid Cells</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Medical genetics</topic><topic>Medical sciences</topic><topic>radiation hybrids</topic><topic>Sequence Tagged Sites</topic><topic>YACs</topic><toplevel>online_resources</toplevel><creatorcontrib>Viggiano, Luigi</creatorcontrib><creatorcontrib>Miolla, Valeria</creatorcontrib><creatorcontrib>Ricco, Angela</creatorcontrib><creatorcontrib>Storlazzi, Clelia Tiziana</creatorcontrib><creatorcontrib>Pennacchia, Margherita</creatorcontrib><creatorcontrib>Fratello, Angelo</creatorcontrib><creatorcontrib>Mancini, Mita</creatorcontrib><creatorcontrib>Marzella, Rosalia</creatorcontrib><creatorcontrib>Archidiacono, Nicoletta</creatorcontrib><creatorcontrib>Rocchi, Mariano</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of medical genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Viggiano, Luigi</au><au>Miolla, Valeria</au><au>Ricco, Angela</au><au>Storlazzi, Clelia Tiziana</au><au>Pennacchia, Margherita</au><au>Fratello, Angelo</au><au>Mancini, Mita</au><au>Marzella, Rosalia</au><au>Archidiacono, Nicoletta</au><au>Rocchi, Mariano</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cytogenetic resources specific for chromosome 12</atitle><jtitle>American journal of medical genetics</jtitle><addtitle>Am. J. Med. Genet</addtitle><date>1999-11-05</date><risdate>1999</risdate><volume>87</volume><issue>1</issue><spage>40</spage><epage>44</epage><pages>40-44</pages><issn>0148-7299</issn><eissn>1096-8628</eissn><coden>AJMGDA</coden><abstract>We have generated a panel of 20 somatic cell hybrids retaining fragments of human chromosome 12. Each hybrid was characterized cytogenetically by reverse fluorescence in situ hybridization (FISH) and molecularly by 24 sequence tagged sites (STSs) spaced evenly along the chromosome. The panel can be exploited to map subregionally DNA sequences on chromosome 12 and to generate partial chromosome paints useful in the characterization of chromosomal rearrangements involving this chromosome. Furthermore, a panel of 58 yeast artificial chromosomes (YACs) mapping to chromosome 12 was characterized by FISH experiments on normal human metaphases. A subset of this panel is recognized by the STSs used in the somatic cell hybrid characterization. In this way a correlation between the genetic and the physical maps of this chromosome can be established. Am. J. Med. Genet. 87:40–44, 1999. © 1999 Wiley‐Liss, Inc.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>10528245</pmid><doi>10.1002/(SICI)1096-8628(19991105)87:1<40::AID-AJMG8>3.0.CO;2-2</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0148-7299 |
| ispartof | American journal of medical genetics, 1999-11, Vol.87 (1), p.40-44 |
| issn | 0148-7299 1096-8628 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70841743 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Biological and medical sciences chromosome 12 Chromosome Mapping Chromosomes, Artificial, Yeast Chromosomes, Human, Pair 12 - genetics Cricetinae Cytogenetic Analysis General aspects. Genetic counseling Humans Hybrid Cells In Situ Hybridization, Fluorescence Medical genetics Medical sciences radiation hybrids Sequence Tagged Sites YACs |
| title | Molecular cytogenetic resources specific for chromosome 12 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T09%3A12%3A57IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Molecular%20cytogenetic%20resources%20specific%20for%20chromosome%2012&rft.jtitle=American%20journal%20of%20medical%20genetics&rft.au=Viggiano,%20Luigi&rft.date=1999-11-05&rft.volume=87&rft.issue=1&rft.spage=40&rft.epage=44&rft.pages=40-44&rft.issn=0148-7299&rft.eissn=1096-8628&rft.coden=AJMGDA&rft_id=info:doi/10.1002/(SICI)1096-8628(19991105)87:1%3C40::AID-AJMG8%3E3.0.CO;2-2&rft_dat=%3Cproquest_cross%3E70841743%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=70841743&rft_id=info:pmid/10528245&rfr_iscdi=true |