Interleukin-2 Expression by a Subpopulation of Primary T Cells Is Linked to Enhanced Memory/Effector Function
Single cell studies have identified intraclonal heterogeneity of cytokine production by activated T cells. To investigate implications of cytokine heterogeneity for cell fate, an interleukin (IL)-2 promoter-green fluorescent protein (GFP) reporter transgenic model was developed to track IL-2 + and I...
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| Veröffentlicht in: | Immunity (Cambridge, Mass.) Mass.), 1999-09, Vol.11 (3), p.271-280 |
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| creator | Saparov, Arman Wagner, Fred H Zheng, Rui Oliver, James R Maeda, Hiroko Hockett, Richard D Weaver, Casey T |
| description | Single cell studies have identified intraclonal heterogeneity of cytokine production by activated T cells. To investigate implications of cytokine heterogeneity for cell fate, an interleukin (IL)-2 promoter-green fluorescent protein (GFP) reporter transgenic model was developed to track IL-2
+ and IL-2
− T cells during differentiation from naive precursors. Antigen-activated IL-2
+ and IL-2
− cells had comparable proliferative capacities in primary responses. However, T cells that expressed IL-2 in primary responses demonstrated enhanced antigenic sensitivity and increased expression of effector cytokines in secondary responses in vitro and in vivo. Thus, heterogeneity of activation during a primary response translates into heterogeneous secondary responses, in which enhanced memory/effector function is linked to cells that previously exceeded an activation threshold associated with IL-2 gene transcription. |
| doi_str_mv | 10.1016/S1074-7613(00)80102-8 |
| format | Article |
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+ and IL-2
− T cells during differentiation from naive precursors. Antigen-activated IL-2
+ and IL-2
− cells had comparable proliferative capacities in primary responses. However, T cells that expressed IL-2 in primary responses demonstrated enhanced antigenic sensitivity and increased expression of effector cytokines in secondary responses in vitro and in vivo. Thus, heterogeneity of activation during a primary response translates into heterogeneous secondary responses, in which enhanced memory/effector function is linked to cells that previously exceeded an activation threshold associated with IL-2 gene transcription.</description><identifier>ISSN: 1074-7613</identifier><identifier>EISSN: 1097-4180</identifier><identifier>DOI: 10.1016/S1074-7613(00)80102-8</identifier><identifier>PMID: 10514005</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>AIDS/HIV ; Animals ; CD2 Antigens - genetics ; Cell Differentiation ; Cells, Cultured ; Female ; Gene Expression ; Green Fluorescent Proteins ; Immunologic Memory - immunology ; Interleukin-2 - biosynthesis ; Interleukin-2 - genetics ; Luminescent Proteins - genetics ; Lymphocyte Subsets - immunology ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Transgenic ; Ovalbumin - immunology ; Promoter Regions, Genetic ; T-Lymphocytes - cytology ; T-Lymphocytes - immunology ; Th1 Cells - cytology ; Th1 Cells - immunology ; Th2 Cells - cytology ; Th2 Cells - immunology ; Transgenes</subject><ispartof>Immunity (Cambridge, Mass.), 1999-09, Vol.11 (3), p.271-280</ispartof><rights>1999 Cell Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c439t-e025fd3d179d1f9e8f8e7edddc4ad2939073e94c6bb5dbeef8b0be8927fd036a3</citedby><cites>FETCH-LOGICAL-c439t-e025fd3d179d1f9e8f8e7edddc4ad2939073e94c6bb5dbeef8b0be8927fd036a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1074761300801028$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10514005$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Saparov, Arman</creatorcontrib><creatorcontrib>Wagner, Fred H</creatorcontrib><creatorcontrib>Zheng, Rui</creatorcontrib><creatorcontrib>Oliver, James R</creatorcontrib><creatorcontrib>Maeda, Hiroko</creatorcontrib><creatorcontrib>Hockett, Richard D</creatorcontrib><creatorcontrib>Weaver, Casey T</creatorcontrib><title>Interleukin-2 Expression by a Subpopulation of Primary T Cells Is Linked to Enhanced Memory/Effector Function</title><title>Immunity (Cambridge, Mass.)</title><addtitle>Immunity</addtitle><description>Single cell studies have identified intraclonal heterogeneity of cytokine production by activated T cells. To investigate implications of cytokine heterogeneity for cell fate, an interleukin (IL)-2 promoter-green fluorescent protein (GFP) reporter transgenic model was developed to track IL-2
+ and IL-2
− T cells during differentiation from naive precursors. Antigen-activated IL-2
+ and IL-2
− cells had comparable proliferative capacities in primary responses. However, T cells that expressed IL-2 in primary responses demonstrated enhanced antigenic sensitivity and increased expression of effector cytokines in secondary responses in vitro and in vivo. Thus, heterogeneity of activation during a primary response translates into heterogeneous secondary responses, in which enhanced memory/effector function is linked to cells that previously exceeded an activation threshold associated with IL-2 gene transcription.</description><subject>AIDS/HIV</subject><subject>Animals</subject><subject>CD2 Antigens - genetics</subject><subject>Cell Differentiation</subject><subject>Cells, Cultured</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Green Fluorescent Proteins</subject><subject>Immunologic Memory - immunology</subject><subject>Interleukin-2 - biosynthesis</subject><subject>Interleukin-2 - genetics</subject><subject>Luminescent Proteins - genetics</subject><subject>Lymphocyte Subsets - immunology</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Transgenic</subject><subject>Ovalbumin - immunology</subject><subject>Promoter Regions, Genetic</subject><subject>T-Lymphocytes - cytology</subject><subject>T-Lymphocytes - immunology</subject><subject>Th1 Cells - cytology</subject><subject>Th1 Cells - immunology</subject><subject>Th2 Cells - cytology</subject><subject>Th2 Cells - immunology</subject><subject>Transgenes</subject><issn>1074-7613</issn><issn>1097-4180</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFP3DAQha2qqFDoTwD5VNFDYBwna_uEqtXSrrRVkaBnK7HHwpDEwU4q9t83IXvojdOMRt_MG71HyDmDKwZsdX3PQBSZWDF-CfBNAoM8kx_ICQMlsoJJ-Dj3B-SYfE7pCYAVpYJP5JhByQqA8oS0227A2OD47Lssp5vXPmJKPnS03tOK3o91H_qxqYZ5FBy9i76t4p4-0DU2TaLbRHe-e0ZLh0A33WPVman_hW2I--uNc2iGEOnt2Jn5whk5clWT8MuhnpI_t5uH9c9s9_vHdv19l5mCqyFDyEtnuWVCWeYUSidRoLXWFJXNFVcgOKrCrOq6tDWikzXUKFUunAW-qvgp-brc7WN4GTENuvXJTA9XHYYxaQGyYBzgXZAJPqlJNoHlApoYUorodL84oRnoORD9Foie3dYA-i0QLae9i4PAWLdo_9taEpiAmwXAyY-_HqNOxuPsoo-TedoG_47EPyv4m6M</recordid><startdate>19990901</startdate><enddate>19990901</enddate><creator>Saparov, Arman</creator><creator>Wagner, Fred H</creator><creator>Zheng, Rui</creator><creator>Oliver, James R</creator><creator>Maeda, Hiroko</creator><creator>Hockett, Richard D</creator><creator>Weaver, Casey T</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19990901</creationdate><title>Interleukin-2 Expression by a Subpopulation of Primary T Cells Is Linked to Enhanced Memory/Effector Function</title><author>Saparov, Arman ; Wagner, Fred H ; Zheng, Rui ; Oliver, James R ; Maeda, Hiroko ; Hockett, Richard D ; Weaver, Casey T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-e025fd3d179d1f9e8f8e7edddc4ad2939073e94c6bb5dbeef8b0be8927fd036a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>AIDS/HIV</topic><topic>Animals</topic><topic>CD2 Antigens - genetics</topic><topic>Cell Differentiation</topic><topic>Cells, Cultured</topic><topic>Female</topic><topic>Gene Expression</topic><topic>Green Fluorescent Proteins</topic><topic>Immunologic Memory - immunology</topic><topic>Interleukin-2 - biosynthesis</topic><topic>Interleukin-2 - genetics</topic><topic>Luminescent Proteins - genetics</topic><topic>Lymphocyte Subsets - immunology</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Transgenic</topic><topic>Ovalbumin - immunology</topic><topic>Promoter Regions, Genetic</topic><topic>T-Lymphocytes - cytology</topic><topic>T-Lymphocytes - immunology</topic><topic>Th1 Cells - cytology</topic><topic>Th1 Cells - immunology</topic><topic>Th2 Cells - cytology</topic><topic>Th2 Cells - immunology</topic><topic>Transgenes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saparov, Arman</creatorcontrib><creatorcontrib>Wagner, Fred H</creatorcontrib><creatorcontrib>Zheng, Rui</creatorcontrib><creatorcontrib>Oliver, James R</creatorcontrib><creatorcontrib>Maeda, Hiroko</creatorcontrib><creatorcontrib>Hockett, Richard D</creatorcontrib><creatorcontrib>Weaver, Casey T</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Immunity (Cambridge, Mass.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saparov, Arman</au><au>Wagner, Fred H</au><au>Zheng, Rui</au><au>Oliver, James R</au><au>Maeda, Hiroko</au><au>Hockett, Richard D</au><au>Weaver, Casey T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Interleukin-2 Expression by a Subpopulation of Primary T Cells Is Linked to Enhanced Memory/Effector Function</atitle><jtitle>Immunity (Cambridge, Mass.)</jtitle><addtitle>Immunity</addtitle><date>1999-09-01</date><risdate>1999</risdate><volume>11</volume><issue>3</issue><spage>271</spage><epage>280</epage><pages>271-280</pages><issn>1074-7613</issn><eissn>1097-4180</eissn><abstract>Single cell studies have identified intraclonal heterogeneity of cytokine production by activated T cells. 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+ and IL-2
− T cells during differentiation from naive precursors. Antigen-activated IL-2
+ and IL-2
− cells had comparable proliferative capacities in primary responses. However, T cells that expressed IL-2 in primary responses demonstrated enhanced antigenic sensitivity and increased expression of effector cytokines in secondary responses in vitro and in vivo. Thus, heterogeneity of activation during a primary response translates into heterogeneous secondary responses, in which enhanced memory/effector function is linked to cells that previously exceeded an activation threshold associated with IL-2 gene transcription.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>10514005</pmid><doi>10.1016/S1074-7613(00)80102-8</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Cell Press Free Archives; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | AIDS/HIV Animals CD2 Antigens - genetics Cell Differentiation Cells, Cultured Female Gene Expression Green Fluorescent Proteins Immunologic Memory - immunology Interleukin-2 - biosynthesis Interleukin-2 - genetics Luminescent Proteins - genetics Lymphocyte Subsets - immunology Male Mice Mice, Inbred BALB C Mice, Transgenic Ovalbumin - immunology Promoter Regions, Genetic T-Lymphocytes - cytology T-Lymphocytes - immunology Th1 Cells - cytology Th1 Cells - immunology Th2 Cells - cytology Th2 Cells - immunology Transgenes |
| title | Interleukin-2 Expression by a Subpopulation of Primary T Cells Is Linked to Enhanced Memory/Effector Function |
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