Platelet c‐mpl expression is dysregulated in patients with essential thrombocythaemia but this is not of diagnostic value
Essential thrombocythaemia (ET) can be difficult to discriminate from an occult case of reactive thrombocytosis (RT). Since thrombopoietin (TPO) is the primary regulator of thrombopoiesis, we have investigated whether levels of TPO and/or its receptor, c‐mpl, are of value in the differential diagnos...
Gespeichert in:
| Veröffentlicht in: | British journal of haematology 1999-10, Vol.107 (1), p.139-147 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 147 |
|---|---|
| container_issue | 1 |
| container_start_page | 139 |
| container_title | British journal of haematology |
| container_volume | 107 |
| creator | Harrison, Claire N. Gale, Rosemary E. Pezella, Francesco Mire‐Sluis, Anthony Machin, Samuel J. Linch, David C. |
| description | Essential thrombocythaemia (ET) can be difficult to discriminate from an occult case of reactive thrombocytosis (RT). Since thrombopoietin (TPO) is the primary regulator of thrombopoiesis, we have investigated whether levels of TPO and/or its receptor, c‐mpl, are of value in the differential diagnosis of ET. Plasma TPO levels in patients with ET, RT and other myeloproliferative disorders (MPDs) did not differ significantly from normal controls. However, surface c‐mpl expression was significantly reduced in platelets from 18 ET patients, 0–65.5% of controls (P |
| doi_str_mv | 10.1046/j.1365-2141.1999.01667.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70840824</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>45953098</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4717-7e399901384361d27e65b045820b7ba45b0fb77e648c8579bfb6264e0b901ac43</originalsourceid><addsrcrecordid>eNqNkc-O1SAUxonRONfRVzDEGHeth5YCXbhwJupoJtGFrglQOpcbWmqhzr1x4yP4jD6J1HujxpUJCZzD7_v48yGECZQEKHu-K0nNmqIilJSkbdsSCGO83N9Bm98bd9EGAHiRBeIMPYhxB0BqaMh9dEagqQBqukFfP3iVrLcJmx_fvg-Tx3Y_zTZGF0bsIu4OcbY3ywp12I14UsnZMUV869IWZy4XTnmctnMYdDCHtFV2cArrJeVmdshjDAmHHndO3YwhJmfwF-UX-xDd65WP9tFpPkefXr_6eHlVXL9_8_by5XVhKCe84LbOT8x3F7RmpKu4ZY0G2ogKNNeK5qLXPHepMKLhre41qxi1oLNIGVqfo2dH32kOnxcbkxxcNNZ7NdqwRMlBUBDVCj75B9yFZR7z3SRpRSMoZXWGxBEyc4j5c3o5zW5Q80ESkGs6cifXEOQaglzTkb_SkfssfXzyX_Rgu7-Exzgy8PQEqGiU72c1Ghf_cC3PdjxjL47YrfP28N_ny4t3V-uq_gm1L6xj</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>198584463</pqid></control><display><type>article</type><title>Platelet c‐mpl expression is dysregulated in patients with essential thrombocythaemia but this is not of diagnostic value</title><source>Wiley Free Content</source><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Harrison, Claire N. ; Gale, Rosemary E. ; Pezella, Francesco ; Mire‐Sluis, Anthony ; Machin, Samuel J. ; Linch, David C.</creator><creatorcontrib>Harrison, Claire N. ; Gale, Rosemary E. ; Pezella, Francesco ; Mire‐Sluis, Anthony ; Machin, Samuel J. ; Linch, David C.</creatorcontrib><description>Essential thrombocythaemia (ET) can be difficult to discriminate from an occult case of reactive thrombocytosis (RT). Since thrombopoietin (TPO) is the primary regulator of thrombopoiesis, we have investigated whether levels of TPO and/or its receptor, c‐mpl, are of value in the differential diagnosis of ET. Plasma TPO levels in patients with ET, RT and other myeloproliferative disorders (MPDs) did not differ significantly from normal controls. However, surface c‐mpl expression was significantly reduced in platelets from 18 ET patients, 0–65.5% of controls (P < 0.001). Immunoblots on five of these and five additional patients were consistent with absent or reduced c‐mpl protein levels. The surface c‐mpl expression results were significantly different from those in eight RT patients (21.3–95.5%, P = 0.0015), but there was considerable overlap between the two groups and a reduced level was not restricted to ET. Furthermore, c‐mpl expression in ET patients was not different from eight patients with other MPDs (0–87.6%, P = 0.06), nor could it differentiate between ET patients with monoclonal and polyclonal haemopoiesis. Although a low or absent c‐mpl level is suggestive of a primary rather than a secondary thrombocytosis, it is insufficiently discriminatory to be used as a diagnostic marker for ET.</description><identifier>ISSN: 0007-1048</identifier><identifier>EISSN: 1365-2141</identifier><identifier>DOI: 10.1046/j.1365-2141.1999.01667.x</identifier><identifier>PMID: 10520034</identifier><identifier>CODEN: BJHEAL</identifier><language>eng</language><publisher>Oxford, U.K. and Cambridge, USA: Blackwell Science Ltd</publisher><subject>Biological and medical sciences ; Blood Platelets - metabolism ; Blotting, Western ; Bone Marrow - pathology ; c‐mpl ; essential thrombocythaemia ; Female ; Hematologic and hematopoietic diseases ; Hematology ; Humans ; Immunohistochemistry ; Male ; Medical sciences ; Platelet Count ; Platelet diseases and coagulopathies ; Thrombocythemia, Essential - blood ; Thrombocythemia, Essential - diagnosis ; thrombocytosis ; thrombopoietin ; Thrombopoietin - metabolism</subject><ispartof>British journal of haematology, 1999-10, Vol.107 (1), p.139-147</ispartof><rights>1999 INIST-CNRS</rights><rights>Copyright Blackwell Scientific Publications Ltd. Oct 1999</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4717-7e399901384361d27e65b045820b7ba45b0fb77e648c8579bfb6264e0b901ac43</citedby><cites>FETCH-LOGICAL-c4717-7e399901384361d27e65b045820b7ba45b0fb77e648c8579bfb6264e0b901ac43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2141.1999.01667.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2141.1999.01667.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1979997$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10520034$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Harrison, Claire N.</creatorcontrib><creatorcontrib>Gale, Rosemary E.</creatorcontrib><creatorcontrib>Pezella, Francesco</creatorcontrib><creatorcontrib>Mire‐Sluis, Anthony</creatorcontrib><creatorcontrib>Machin, Samuel J.</creatorcontrib><creatorcontrib>Linch, David C.</creatorcontrib><title>Platelet c‐mpl expression is dysregulated in patients with essential thrombocythaemia but this is not of diagnostic value</title><title>British journal of haematology</title><addtitle>Br J Haematol</addtitle><description>Essential thrombocythaemia (ET) can be difficult to discriminate from an occult case of reactive thrombocytosis (RT). Since thrombopoietin (TPO) is the primary regulator of thrombopoiesis, we have investigated whether levels of TPO and/or its receptor, c‐mpl, are of value in the differential diagnosis of ET. Plasma TPO levels in patients with ET, RT and other myeloproliferative disorders (MPDs) did not differ significantly from normal controls. However, surface c‐mpl expression was significantly reduced in platelets from 18 ET patients, 0–65.5% of controls (P < 0.001). Immunoblots on five of these and five additional patients were consistent with absent or reduced c‐mpl protein levels. The surface c‐mpl expression results were significantly different from those in eight RT patients (21.3–95.5%, P = 0.0015), but there was considerable overlap between the two groups and a reduced level was not restricted to ET. Furthermore, c‐mpl expression in ET patients was not different from eight patients with other MPDs (0–87.6%, P = 0.06), nor could it differentiate between ET patients with monoclonal and polyclonal haemopoiesis. Although a low or absent c‐mpl level is suggestive of a primary rather than a secondary thrombocytosis, it is insufficiently discriminatory to be used as a diagnostic marker for ET.</description><subject>Biological and medical sciences</subject><subject>Blood Platelets - metabolism</subject><subject>Blotting, Western</subject><subject>Bone Marrow - pathology</subject><subject>c‐mpl</subject><subject>essential thrombocythaemia</subject><subject>Female</subject><subject>Hematologic and hematopoietic diseases</subject><subject>Hematology</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Platelet Count</subject><subject>Platelet diseases and coagulopathies</subject><subject>Thrombocythemia, Essential - blood</subject><subject>Thrombocythemia, Essential - diagnosis</subject><subject>thrombocytosis</subject><subject>thrombopoietin</subject><subject>Thrombopoietin - metabolism</subject><issn>0007-1048</issn><issn>1365-2141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc-O1SAUxonRONfRVzDEGHeth5YCXbhwJupoJtGFrglQOpcbWmqhzr1x4yP4jD6J1HujxpUJCZzD7_v48yGECZQEKHu-K0nNmqIilJSkbdsSCGO83N9Bm98bd9EGAHiRBeIMPYhxB0BqaMh9dEagqQBqukFfP3iVrLcJmx_fvg-Tx3Y_zTZGF0bsIu4OcbY3ywp12I14UsnZMUV869IWZy4XTnmctnMYdDCHtFV2cArrJeVmdshjDAmHHndO3YwhJmfwF-UX-xDd65WP9tFpPkefXr_6eHlVXL9_8_by5XVhKCe84LbOT8x3F7RmpKu4ZY0G2ogKNNeK5qLXPHepMKLhre41qxi1oLNIGVqfo2dH32kOnxcbkxxcNNZ7NdqwRMlBUBDVCj75B9yFZR7z3SRpRSMoZXWGxBEyc4j5c3o5zW5Q80ESkGs6cifXEOQaglzTkb_SkfssfXzyX_Rgu7-Exzgy8PQEqGiU72c1Ghf_cC3PdjxjL47YrfP28N_ny4t3V-uq_gm1L6xj</recordid><startdate>199910</startdate><enddate>199910</enddate><creator>Harrison, Claire N.</creator><creator>Gale, Rosemary E.</creator><creator>Pezella, Francesco</creator><creator>Mire‐Sluis, Anthony</creator><creator>Machin, Samuel J.</creator><creator>Linch, David C.</creator><general>Blackwell Science Ltd</general><general>Blackwell</general><general>Blackwell Publishing Ltd</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>199910</creationdate><title>Platelet c‐mpl expression is dysregulated in patients with essential thrombocythaemia but this is not of diagnostic value</title><author>Harrison, Claire N. ; Gale, Rosemary E. ; Pezella, Francesco ; Mire‐Sluis, Anthony ; Machin, Samuel J. ; Linch, David C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4717-7e399901384361d27e65b045820b7ba45b0fb77e648c8579bfb6264e0b901ac43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Biological and medical sciences</topic><topic>Blood Platelets - metabolism</topic><topic>Blotting, Western</topic><topic>Bone Marrow - pathology</topic><topic>c‐mpl</topic><topic>essential thrombocythaemia</topic><topic>Female</topic><topic>Hematologic and hematopoietic diseases</topic><topic>Hematology</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Platelet Count</topic><topic>Platelet diseases and coagulopathies</topic><topic>Thrombocythemia, Essential - blood</topic><topic>Thrombocythemia, Essential - diagnosis</topic><topic>thrombocytosis</topic><topic>thrombopoietin</topic><topic>Thrombopoietin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Harrison, Claire N.</creatorcontrib><creatorcontrib>Gale, Rosemary E.</creatorcontrib><creatorcontrib>Pezella, Francesco</creatorcontrib><creatorcontrib>Mire‐Sluis, Anthony</creatorcontrib><creatorcontrib>Machin, Samuel J.</creatorcontrib><creatorcontrib>Linch, David C.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>British journal of haematology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Harrison, Claire N.</au><au>Gale, Rosemary E.</au><au>Pezella, Francesco</au><au>Mire‐Sluis, Anthony</au><au>Machin, Samuel J.</au><au>Linch, David C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Platelet c‐mpl expression is dysregulated in patients with essential thrombocythaemia but this is not of diagnostic value</atitle><jtitle>British journal of haematology</jtitle><addtitle>Br J Haematol</addtitle><date>1999-10</date><risdate>1999</risdate><volume>107</volume><issue>1</issue><spage>139</spage><epage>147</epage><pages>139-147</pages><issn>0007-1048</issn><eissn>1365-2141</eissn><coden>BJHEAL</coden><abstract>Essential thrombocythaemia (ET) can be difficult to discriminate from an occult case of reactive thrombocytosis (RT). Since thrombopoietin (TPO) is the primary regulator of thrombopoiesis, we have investigated whether levels of TPO and/or its receptor, c‐mpl, are of value in the differential diagnosis of ET. Plasma TPO levels in patients with ET, RT and other myeloproliferative disorders (MPDs) did not differ significantly from normal controls. However, surface c‐mpl expression was significantly reduced in platelets from 18 ET patients, 0–65.5% of controls (P < 0.001). Immunoblots on five of these and five additional patients were consistent with absent or reduced c‐mpl protein levels. The surface c‐mpl expression results were significantly different from those in eight RT patients (21.3–95.5%, P = 0.0015), but there was considerable overlap between the two groups and a reduced level was not restricted to ET. Furthermore, c‐mpl expression in ET patients was not different from eight patients with other MPDs (0–87.6%, P = 0.06), nor could it differentiate between ET patients with monoclonal and polyclonal haemopoiesis. Although a low or absent c‐mpl level is suggestive of a primary rather than a secondary thrombocytosis, it is insufficiently discriminatory to be used as a diagnostic marker for ET.</abstract><cop>Oxford, U.K. and Cambridge, USA</cop><pub>Blackwell Science Ltd</pub><pmid>10520034</pmid><doi>10.1046/j.1365-2141.1999.01667.x</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0007-1048 |
| ispartof | British journal of haematology, 1999-10, Vol.107 (1), p.139-147 |
| issn | 0007-1048 1365-2141 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70840824 |
| source | Wiley Free Content; MEDLINE; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Biological and medical sciences Blood Platelets - metabolism Blotting, Western Bone Marrow - pathology c‐mpl essential thrombocythaemia Female Hematologic and hematopoietic diseases Hematology Humans Immunohistochemistry Male Medical sciences Platelet Count Platelet diseases and coagulopathies Thrombocythemia, Essential - blood Thrombocythemia, Essential - diagnosis thrombocytosis thrombopoietin Thrombopoietin - metabolism |
| title | Platelet c‐mpl expression is dysregulated in patients with essential thrombocythaemia but this is not of diagnostic value |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T02%3A17%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Platelet%20c%E2%80%90mpl%20expression%20is%20dysregulated%20in%20patients%20with%20essential%20thrombocythaemia%20but%20this%20is%20not%20of%20diagnostic%20value&rft.jtitle=British%20journal%20of%20haematology&rft.au=Harrison,%20Claire%20N.&rft.date=1999-10&rft.volume=107&rft.issue=1&rft.spage=139&rft.epage=147&rft.pages=139-147&rft.issn=0007-1048&rft.eissn=1365-2141&rft.coden=BJHEAL&rft_id=info:doi/10.1046/j.1365-2141.1999.01667.x&rft_dat=%3Cproquest_cross%3E45953098%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=198584463&rft_id=info:pmid/10520034&rfr_iscdi=true |