Multiplex analysis of heterophil antibodies in patients with indeterminate HIV immunoassay results

We hypothesized that heterophil antibodies reactive with animal proteins used in blot preparation caused nonspecific staining (NSS) on HIV Western blot (WB) studies, causing indeterminate results. We analyzed samples showing NSS on HIV WB using a multiplexed immunoassay to simultaneously measure IgG...

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Veröffentlicht in:	American journal of clinical pathology 2001-05, Vol.115 (5), p.764-769
Hauptverfasser:	WILLMAN, Joseph H, HILL, Harry R, MARTINS, Thomas B, JASKOWSKI, Troy D, ASHWOOD, Edward R, LITWIN, Christine M
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Heterophile - analysis Antibodies, Heterophile - immunology Antibody Specificity Antigens, Viral - analysis Antigens, Viral - immunology Biological and medical sciences Blotting, Western Cattle - immunology Cross Reactions Enzyme-Linked Immunosorbent Assay False Positive Reactions Goats - immunology HIV Antibodies - analysis HIV Antibodies - immunology HIV Seropositivity - diagnosis HIV Seropositivity - immunology HIV-1 - immunology Human immunodeficiency virus Humans Immunoassay - methods Investigative techniques, diagnostic techniques (general aspects) Medical sciences Mice - immunology Microspheres Miscellaneous. Technology Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Serum Albumin, Bovine - immunology Sheep - immunology
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