Ovarian Function in the Giant Tiger Prawn (Penaeus monodon) as Determined by In Vitro Bioassay
Ovary tissue fragments of the giant tiger prawnPenaeus monodonwere incubated in vitro withl‐methionine[35S] plusl‐cysteine[35S] as a metabolic labeling reagent. The labeled cytoplasmic and secreted proteins synthesized in vitro during incubations under various conditions were subjected to SDS polyac...
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| Veröffentlicht in: | Physiological and biochemical zoology 1999-09, Vol.72 (5), p.588-596 |
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| container_title | Physiological and biochemical zoology |
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| creator | Thurn, Michael J. Hall, Michael R. |
| description | Ovary tissue fragments of the giant tiger prawnPenaeus monodonwere incubated in vitro withl‐methionine[35S] plusl‐cysteine[35S] as a metabolic labeling reagent. The labeled cytoplasmic and secreted proteins synthesized in vitro during incubations under various conditions were subjected to SDS polyacrylamide electrophoresis and visualized by autoradiography. Vitellogenin (Vg) was immunologically identified and shown to be actively synthesized and released into the incubation medium. The synthesis and release of Vg into the incubation medium was optimized and shown to be linear over a 16‐h period. Comparisons between different ovarian regions and different stages of development revealed that the level of Vg synthesis and accumulation in the incubation media was variable depending on stage of development and region within the ovary. Coincubation of ovarian fragments with sinus gland extracts showed a dose‐related inhibition of total protein and Vg synthesis. The in vitro ovarian bioassay is suitable for examining the effect of hormonal inputs ofP. monodon. |
| doi_str_mv | 10.1086/316700 |
| format | Article |
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The labeled cytoplasmic and secreted proteins synthesized in vitro during incubations under various conditions were subjected to SDS polyacrylamide electrophoresis and visualized by autoradiography. Vitellogenin (Vg) was immunologically identified and shown to be actively synthesized and released into the incubation medium. The synthesis and release of Vg into the incubation medium was optimized and shown to be linear over a 16‐h period. Comparisons between different ovarian regions and different stages of development revealed that the level of Vg synthesis and accumulation in the incubation media was variable depending on stage of development and region within the ovary. Coincubation of ovarian fragments with sinus gland extracts showed a dose‐related inhibition of total protein and Vg synthesis. The in vitro ovarian bioassay is suitable for examining the effect of hormonal inputs ofP. monodon.</description><identifier>ISSN: 1522-2152</identifier><identifier>EISSN: 1537-5293</identifier><identifier>DOI: 10.1086/316700</identifier><identifier>PMID: 10521326</identifier><language>eng</language><publisher>United States: The University of Chicago Press</publisher><subject>Animals ; Biological Assay - methods ; Crustaceans ; Cysteine - metabolism ; Endocrine glands ; Female ; Gels ; Hormones ; Methionine - metabolism ; Oocytes ; Ovarian development ; Ovaries ; Ovary - physiology ; Penaeidae - physiology ; Protein synthesis ; Shrimp ; Sulfur Radioisotopes ; Vitellogenesis ; Vitellogenins - biosynthesis ; Vitellogenins - secretion</subject><ispartof>Physiological and biochemical zoology, 1999-09, Vol.72 (5), p.588-596</ispartof><rights>1999 by The University of Chicago. All rights reserved.</rights><rights>1999 by The University of Chicago. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c421t-fe193cfcdd884a60be2aa368ba2a0408cd29241f5b1c7a4d4fd4fc12e00823a63</citedby><cites>FETCH-LOGICAL-c421t-fe193cfcdd884a60be2aa368ba2a0408cd29241f5b1c7a4d4fd4fc12e00823a63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,799,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10521326$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Thurn, Michael J.</creatorcontrib><creatorcontrib>Hall, Michael R.</creatorcontrib><title>Ovarian Function in the Giant Tiger Prawn (Penaeus monodon) as Determined by In Vitro Bioassay</title><title>Physiological and biochemical zoology</title><addtitle>Physiol Biochem Zool</addtitle><description>Ovary tissue fragments of the giant tiger prawnPenaeus monodonwere incubated in vitro withl‐methionine[35S] plusl‐cysteine[35S] as a metabolic labeling reagent. The labeled cytoplasmic and secreted proteins synthesized in vitro during incubations under various conditions were subjected to SDS polyacrylamide electrophoresis and visualized by autoradiography. Vitellogenin (Vg) was immunologically identified and shown to be actively synthesized and released into the incubation medium. The synthesis and release of Vg into the incubation medium was optimized and shown to be linear over a 16‐h period. Comparisons between different ovarian regions and different stages of development revealed that the level of Vg synthesis and accumulation in the incubation media was variable depending on stage of development and region within the ovary. Coincubation of ovarian fragments with sinus gland extracts showed a dose‐related inhibition of total protein and Vg synthesis. The in vitro ovarian bioassay is suitable for examining the effect of hormonal inputs ofP. monodon.</description><subject>Animals</subject><subject>Biological Assay - methods</subject><subject>Crustaceans</subject><subject>Cysteine - metabolism</subject><subject>Endocrine glands</subject><subject>Female</subject><subject>Gels</subject><subject>Hormones</subject><subject>Methionine - metabolism</subject><subject>Oocytes</subject><subject>Ovarian development</subject><subject>Ovaries</subject><subject>Ovary - physiology</subject><subject>Penaeidae - physiology</subject><subject>Protein synthesis</subject><subject>Shrimp</subject><subject>Sulfur Radioisotopes</subject><subject>Vitellogenesis</subject><subject>Vitellogenins - biosynthesis</subject><subject>Vitellogenins - secretion</subject><issn>1522-2152</issn><issn>1537-5293</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkdtKxDAQhoMonn0DJRcielGdJD3tpWcFYfdi9dIyTaeaZZusSavs21vpokKYGYaPD-YPYwcCzgXk6YUSaQawxrZForIokSO1_jNLGcm-brGdEGYAQuQw2mRbAhIplEy32ev4E71By-86q1vjLDeWt-_E7_tly6fmjTyfePyy_HRCFqkLvHHWVc6ecQz8hlryjbFU8XLJHy1_Ma13_Mo4DAGXe2yjxnmg_VXfZc93t9Prh-hpfP94ffkU6ViKNqpJjJSudVXleYwplCQRVZqXKBFiyHUlRzIWdVIKnWFcxXX_tJAEkEuFqdplJ4N34d1HR6EtGhM0zedoyXWhyCBXWZLFf6D2LgRPdbHwpkG_LAQUP0kWQ5I9eLQydmVD1T9siK4Hjgeg0-9G45tbeAqhmLnO2_7UP8_hgM1C6_yvRoFIAfpv-gZJbILq</recordid><startdate>19990901</startdate><enddate>19990901</enddate><creator>Thurn, Michael J.</creator><creator>Hall, Michael R.</creator><general>The University of Chicago Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990901</creationdate><title>Ovarian Function in the Giant Tiger Prawn (Penaeus monodon) as Determined by In Vitro Bioassay</title><author>Thurn, Michael J. ; Hall, Michael R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c421t-fe193cfcdd884a60be2aa368ba2a0408cd29241f5b1c7a4d4fd4fc12e00823a63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Biological Assay - methods</topic><topic>Crustaceans</topic><topic>Cysteine - metabolism</topic><topic>Endocrine glands</topic><topic>Female</topic><topic>Gels</topic><topic>Hormones</topic><topic>Methionine - metabolism</topic><topic>Oocytes</topic><topic>Ovarian development</topic><topic>Ovaries</topic><topic>Ovary - physiology</topic><topic>Penaeidae - physiology</topic><topic>Protein synthesis</topic><topic>Shrimp</topic><topic>Sulfur Radioisotopes</topic><topic>Vitellogenesis</topic><topic>Vitellogenins - biosynthesis</topic><topic>Vitellogenins - secretion</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thurn, Michael J.</creatorcontrib><creatorcontrib>Hall, Michael R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Physiological and biochemical zoology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thurn, Michael J.</au><au>Hall, Michael R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ovarian Function in the Giant Tiger Prawn (Penaeus monodon) as Determined by In Vitro Bioassay</atitle><jtitle>Physiological and biochemical zoology</jtitle><addtitle>Physiol Biochem Zool</addtitle><date>1999-09-01</date><risdate>1999</risdate><volume>72</volume><issue>5</issue><spage>588</spage><epage>596</epage><pages>588-596</pages><issn>1522-2152</issn><eissn>1537-5293</eissn><abstract>Ovary tissue fragments of the giant tiger prawnPenaeus monodonwere incubated in vitro withl‐methionine[35S] plusl‐cysteine[35S] as a metabolic labeling reagent. The labeled cytoplasmic and secreted proteins synthesized in vitro during incubations under various conditions were subjected to SDS polyacrylamide electrophoresis and visualized by autoradiography. Vitellogenin (Vg) was immunologically identified and shown to be actively synthesized and released into the incubation medium. The synthesis and release of Vg into the incubation medium was optimized and shown to be linear over a 16‐h period. Comparisons between different ovarian regions and different stages of development revealed that the level of Vg synthesis and accumulation in the incubation media was variable depending on stage of development and region within the ovary. Coincubation of ovarian fragments with sinus gland extracts showed a dose‐related inhibition of total protein and Vg synthesis. The in vitro ovarian bioassay is suitable for examining the effect of hormonal inputs ofP. monodon.</abstract><cop>United States</cop><pub>The University of Chicago Press</pub><pmid>10521326</pmid><doi>10.1086/316700</doi><tpages>9</tpages></addata></record> |
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| ispartof | Physiological and biochemical zoology, 1999-09, Vol.72 (5), p.588-596 |
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| source | Jstor Complete Legacy; MEDLINE |
| subjects | Animals Biological Assay - methods Crustaceans Cysteine - metabolism Endocrine glands Female Gels Hormones Methionine - metabolism Oocytes Ovarian development Ovaries Ovary - physiology Penaeidae - physiology Protein synthesis Shrimp Sulfur Radioisotopes Vitellogenesis Vitellogenins - biosynthesis Vitellogenins - secretion |
| title | Ovarian Function in the Giant Tiger Prawn (Penaeus monodon) as Determined by In Vitro Bioassay |
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