T-cell co-stimulatory molecules are upregulated on intestinal macrophages from inflammatory bowel disease mucosa
Macrophages play an important role during mucosal inflammation in inflammatory bowel disease (IBD). As the co-stimulatory molecules B7-1 (CD80) and B7-2 (CD86) play an integral role in the activation of T cells by antigen-presenting cells (APC) we investigated the surface expression of B7-1 and B7-2...
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| Veröffentlicht in: | European journal of gastroenterology & hepatology 1999-10, Vol.11 (10), p.1105-1112 |
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| container_title | European journal of gastroenterology & hepatology |
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| creator | Rogler, Gerhard Hausmann, Martin Spöttl, Tanja Vogl, Daniela Aschenbrenner, Elisabeth Andus, Tilo Falk, Werner Schölmerich, Jürgen Gross, Volker |
| description | Macrophages play an important role during mucosal inflammation in inflammatory bowel disease (IBD). As the co-stimulatory molecules B7-1 (CD80) and B7-2 (CD86) play an integral role in the activation of T cells by antigen-presenting cells (APC) we investigated the surface expression of B7-1 and B7-2 on colonic macrophages from normal and IBD mucosa.
Intestinal macrophages were isolated from biopsies of 13 control persons and 14 patients with IBD (seven with Crohn's disease (CD); and seven with ulcerative colitis (UC)). Cells were characterized by triple fluorescence flow cytometrical analysis using CD33 as macrophage marker.
The expression of B7-1 (CD80) (9.2% +/- 4.2%) and B7-2 (CD86) (15.1% +/- 7.3%) was low on colonic macrophages from normal mucosa, indicating only a low antigen presenting potential. However, on macrophages from IBD colon there was a significant increase in the expression of co-stimulatory molecules (CD80, 33.8% +/- 8.9%, P = 0.00005 vs. control; CD86, 39.9% +/- 8.8%, P = 0.00002). There was no significant difference between CD and UC in the expression of CD80 (CD, 31.3% +/- 6.7%; UC, 34.4% +/- 13.3%) and CD86 (CD, 41.9% +/- 3.8%; UC, 35.6% +/- 13.8%). While in normal mucosa only 10.6% +/- 4.9% of the macrophages expressed CD14, more than 90% of the CD86/CD80 positive cells of the inflamed mucosa were positive for CD14.
Colonic macrophages from normal mucosa rarely express the co-stimulatory molecules CD80 and CD86. In IBD a new macrophage population is found with high expression of co-stimulatory molecules presumably responsible for the perpetuated immune response. |
| doi_str_mv | 10.1097/00042737-199910000-00006 |
| format | Article |
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Intestinal macrophages were isolated from biopsies of 13 control persons and 14 patients with IBD (seven with Crohn's disease (CD); and seven with ulcerative colitis (UC)). Cells were characterized by triple fluorescence flow cytometrical analysis using CD33 as macrophage marker.
The expression of B7-1 (CD80) (9.2% +/- 4.2%) and B7-2 (CD86) (15.1% +/- 7.3%) was low on colonic macrophages from normal mucosa, indicating only a low antigen presenting potential. However, on macrophages from IBD colon there was a significant increase in the expression of co-stimulatory molecules (CD80, 33.8% +/- 8.9%, P = 0.00005 vs. control; CD86, 39.9% +/- 8.8%, P = 0.00002). There was no significant difference between CD and UC in the expression of CD80 (CD, 31.3% +/- 6.7%; UC, 34.4% +/- 13.3%) and CD86 (CD, 41.9% +/- 3.8%; UC, 35.6% +/- 13.8%). While in normal mucosa only 10.6% +/- 4.9% of the macrophages expressed CD14, more than 90% of the CD86/CD80 positive cells of the inflamed mucosa were positive for CD14.
Colonic macrophages from normal mucosa rarely express the co-stimulatory molecules CD80 and CD86. In IBD a new macrophage population is found with high expression of co-stimulatory molecules presumably responsible for the perpetuated immune response.</description><identifier>ISSN: 0954-691X</identifier><identifier>EISSN: 1473-5687</identifier><identifier>DOI: 10.1097/00042737-199910000-00006</identifier><identifier>PMID: 10524639</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott Williams & Wilkins, Inc</publisher><subject>AIDS/HIV ; Antigens, CD - biosynthesis ; Antigens, Differentiation, Myelomonocytic - biosynthesis ; B7-1 Antigen - biosynthesis ; B7-2 Antigen ; Biological and medical sciences ; Cell Count ; Cell Differentiation ; Cells, Cultured ; Dendritic Cells - cytology ; Dendritic Cells - immunology ; Digestive system ; Flow Cytometry ; Humans ; Inflammation - immunology ; Inflammatory Bowel Diseases - immunology ; Intestinal Mucosa - immunology ; Investigative techniques, diagnostic techniques (general aspects) ; Lipopolysaccharide Receptors - biosynthesis ; Macrophages - cytology ; Macrophages - immunology ; Medical sciences ; Membrane Glycoproteins - biosynthesis ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Receptors, IgG - biosynthesis ; Sialic Acid Binding Ig-like Lectin 3 ; Th1 Cells - immunology ; Th2 Cells - immunology</subject><ispartof>European journal of gastroenterology & hepatology, 1999-10, Vol.11 (10), p.1105-1112</ispartof><rights>1999 Lippincott Williams & Wilkins, Inc.</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3176-1df059d41b20e68e67b1993272919a9d59f82fa285d3879e4ae1d6a7f25303783</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1968237$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10524639$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rogler, Gerhard</creatorcontrib><creatorcontrib>Hausmann, Martin</creatorcontrib><creatorcontrib>Spöttl, Tanja</creatorcontrib><creatorcontrib>Vogl, Daniela</creatorcontrib><creatorcontrib>Aschenbrenner, Elisabeth</creatorcontrib><creatorcontrib>Andus, Tilo</creatorcontrib><creatorcontrib>Falk, Werner</creatorcontrib><creatorcontrib>Schölmerich, Jürgen</creatorcontrib><creatorcontrib>Gross, Volker</creatorcontrib><title>T-cell co-stimulatory molecules are upregulated on intestinal macrophages from inflammatory bowel disease mucosa</title><title>European journal of gastroenterology & hepatology</title><addtitle>Eur J Gastroenterol Hepatol</addtitle><description>Macrophages play an important role during mucosal inflammation in inflammatory bowel disease (IBD). As the co-stimulatory molecules B7-1 (CD80) and B7-2 (CD86) play an integral role in the activation of T cells by antigen-presenting cells (APC) we investigated the surface expression of B7-1 and B7-2 on colonic macrophages from normal and IBD mucosa.
Intestinal macrophages were isolated from biopsies of 13 control persons and 14 patients with IBD (seven with Crohn's disease (CD); and seven with ulcerative colitis (UC)). Cells were characterized by triple fluorescence flow cytometrical analysis using CD33 as macrophage marker.
The expression of B7-1 (CD80) (9.2% +/- 4.2%) and B7-2 (CD86) (15.1% +/- 7.3%) was low on colonic macrophages from normal mucosa, indicating only a low antigen presenting potential. However, on macrophages from IBD colon there was a significant increase in the expression of co-stimulatory molecules (CD80, 33.8% +/- 8.9%, P = 0.00005 vs. control; CD86, 39.9% +/- 8.8%, P = 0.00002). There was no significant difference between CD and UC in the expression of CD80 (CD, 31.3% +/- 6.7%; UC, 34.4% +/- 13.3%) and CD86 (CD, 41.9% +/- 3.8%; UC, 35.6% +/- 13.8%). While in normal mucosa only 10.6% +/- 4.9% of the macrophages expressed CD14, more than 90% of the CD86/CD80 positive cells of the inflamed mucosa were positive for CD14.
Colonic macrophages from normal mucosa rarely express the co-stimulatory molecules CD80 and CD86. In IBD a new macrophage population is found with high expression of co-stimulatory molecules presumably responsible for the perpetuated immune response.</description><subject>AIDS/HIV</subject><subject>Antigens, CD - biosynthesis</subject><subject>Antigens, Differentiation, Myelomonocytic - biosynthesis</subject><subject>B7-1 Antigen - biosynthesis</subject><subject>B7-2 Antigen</subject><subject>Biological and medical sciences</subject><subject>Cell Count</subject><subject>Cell Differentiation</subject><subject>Cells, Cultured</subject><subject>Dendritic Cells - cytology</subject><subject>Dendritic Cells - immunology</subject><subject>Digestive system</subject><subject>Flow Cytometry</subject><subject>Humans</subject><subject>Inflammation - immunology</subject><subject>Inflammatory Bowel Diseases - immunology</subject><subject>Intestinal Mucosa - immunology</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Lipopolysaccharide Receptors - biosynthesis</subject><subject>Macrophages - cytology</subject><subject>Macrophages - immunology</subject><subject>Medical sciences</subject><subject>Membrane Glycoproteins - biosynthesis</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Receptors, IgG - biosynthesis</subject><subject>Sialic Acid Binding Ig-like Lectin 3</subject><subject>Th1 Cells - immunology</subject><subject>Th2 Cells - immunology</subject><issn>0954-691X</issn><issn>1473-5687</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU1v1DAQhi0EotvCX0A-IG4GfyT-OKKKAlIlLkXiZs0mk27AjoOdaNV_j5cslAsXj6x55uN9hxAq-FvBnXnHOW-kUYYJ55yoP85Oj35CdqIxirXamqdkx13bMO3EtwtyWcp3zoVRwjwnF4K3stHK7ch8xzoMgXaJlWWMa4Al5QcaU8BuDVgoZKTrnPH-lMKepomO04IVniDQCF1O8wHuKznkFGtuCBDj1mWfjhhoPxaEgjSuXSrwgjwbIBR8eY5X5OvNh7vrT-z2y8fP1-9vWVdX1Ez0A29d34i95KgtarOvWpU00gkHrm_dYOUA0ra9ssZhAyh6DWaQreLKWHVF3mx955x-rnVfH8dykgoTprV4w63S1okK2g2sSkrJOPg5jxHygxfcn9z2f9z2f932v92upa_OM9Z9xP6fws3eCrw-A1A6CEOGqRvLI-e0lcpUrNmwYwoL5vIjrEfM_oAQloP_37HVL45kmFo</recordid><startdate>199910</startdate><enddate>199910</enddate><creator>Rogler, Gerhard</creator><creator>Hausmann, Martin</creator><creator>Spöttl, Tanja</creator><creator>Vogl, Daniela</creator><creator>Aschenbrenner, Elisabeth</creator><creator>Andus, Tilo</creator><creator>Falk, Werner</creator><creator>Schölmerich, Jürgen</creator><creator>Gross, Volker</creator><general>Lippincott Williams & Wilkins, Inc</general><general>Lippincott Williams & Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199910</creationdate><title>T-cell co-stimulatory molecules are upregulated on intestinal macrophages from inflammatory bowel disease mucosa</title><author>Rogler, Gerhard ; Hausmann, Martin ; Spöttl, Tanja ; Vogl, Daniela ; Aschenbrenner, Elisabeth ; Andus, Tilo ; Falk, Werner ; Schölmerich, Jürgen ; Gross, Volker</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3176-1df059d41b20e68e67b1993272919a9d59f82fa285d3879e4ae1d6a7f25303783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>AIDS/HIV</topic><topic>Antigens, CD - biosynthesis</topic><topic>Antigens, Differentiation, Myelomonocytic - biosynthesis</topic><topic>B7-1 Antigen - biosynthesis</topic><topic>B7-2 Antigen</topic><topic>Biological and medical sciences</topic><topic>Cell Count</topic><topic>Cell Differentiation</topic><topic>Cells, Cultured</topic><topic>Dendritic Cells - cytology</topic><topic>Dendritic Cells - immunology</topic><topic>Digestive system</topic><topic>Flow Cytometry</topic><topic>Humans</topic><topic>Inflammation - immunology</topic><topic>Inflammatory Bowel Diseases - immunology</topic><topic>Intestinal Mucosa - immunology</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Lipopolysaccharide Receptors - biosynthesis</topic><topic>Macrophages - cytology</topic><topic>Macrophages - immunology</topic><topic>Medical sciences</topic><topic>Membrane Glycoproteins - biosynthesis</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Receptors, IgG - biosynthesis</topic><topic>Sialic Acid Binding Ig-like Lectin 3</topic><topic>Th1 Cells - immunology</topic><topic>Th2 Cells - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rogler, Gerhard</creatorcontrib><creatorcontrib>Hausmann, Martin</creatorcontrib><creatorcontrib>Spöttl, Tanja</creatorcontrib><creatorcontrib>Vogl, Daniela</creatorcontrib><creatorcontrib>Aschenbrenner, Elisabeth</creatorcontrib><creatorcontrib>Andus, Tilo</creatorcontrib><creatorcontrib>Falk, Werner</creatorcontrib><creatorcontrib>Schölmerich, Jürgen</creatorcontrib><creatorcontrib>Gross, Volker</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of gastroenterology & hepatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rogler, Gerhard</au><au>Hausmann, Martin</au><au>Spöttl, Tanja</au><au>Vogl, Daniela</au><au>Aschenbrenner, Elisabeth</au><au>Andus, Tilo</au><au>Falk, Werner</au><au>Schölmerich, Jürgen</au><au>Gross, Volker</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>T-cell co-stimulatory molecules are upregulated on intestinal macrophages from inflammatory bowel disease mucosa</atitle><jtitle>European journal of gastroenterology & hepatology</jtitle><addtitle>Eur J Gastroenterol Hepatol</addtitle><date>1999-10</date><risdate>1999</risdate><volume>11</volume><issue>10</issue><spage>1105</spage><epage>1112</epage><pages>1105-1112</pages><issn>0954-691X</issn><eissn>1473-5687</eissn><abstract>Macrophages play an important role during mucosal inflammation in inflammatory bowel disease (IBD). As the co-stimulatory molecules B7-1 (CD80) and B7-2 (CD86) play an integral role in the activation of T cells by antigen-presenting cells (APC) we investigated the surface expression of B7-1 and B7-2 on colonic macrophages from normal and IBD mucosa.
Intestinal macrophages were isolated from biopsies of 13 control persons and 14 patients with IBD (seven with Crohn's disease (CD); and seven with ulcerative colitis (UC)). Cells were characterized by triple fluorescence flow cytometrical analysis using CD33 as macrophage marker.
The expression of B7-1 (CD80) (9.2% +/- 4.2%) and B7-2 (CD86) (15.1% +/- 7.3%) was low on colonic macrophages from normal mucosa, indicating only a low antigen presenting potential. However, on macrophages from IBD colon there was a significant increase in the expression of co-stimulatory molecules (CD80, 33.8% +/- 8.9%, P = 0.00005 vs. control; CD86, 39.9% +/- 8.8%, P = 0.00002). There was no significant difference between CD and UC in the expression of CD80 (CD, 31.3% +/- 6.7%; UC, 34.4% +/- 13.3%) and CD86 (CD, 41.9% +/- 3.8%; UC, 35.6% +/- 13.8%). While in normal mucosa only 10.6% +/- 4.9% of the macrophages expressed CD14, more than 90% of the CD86/CD80 positive cells of the inflamed mucosa were positive for CD14.
Colonic macrophages from normal mucosa rarely express the co-stimulatory molecules CD80 and CD86. In IBD a new macrophage population is found with high expression of co-stimulatory molecules presumably responsible for the perpetuated immune response.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott Williams & Wilkins, Inc</pub><pmid>10524639</pmid><doi>10.1097/00042737-199910000-00006</doi><tpages>8</tpages></addata></record> |
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| subjects | AIDS/HIV Antigens, CD - biosynthesis Antigens, Differentiation, Myelomonocytic - biosynthesis B7-1 Antigen - biosynthesis B7-2 Antigen Biological and medical sciences Cell Count Cell Differentiation Cells, Cultured Dendritic Cells - cytology Dendritic Cells - immunology Digestive system Flow Cytometry Humans Inflammation - immunology Inflammatory Bowel Diseases - immunology Intestinal Mucosa - immunology Investigative techniques, diagnostic techniques (general aspects) Lipopolysaccharide Receptors - biosynthesis Macrophages - cytology Macrophages - immunology Medical sciences Membrane Glycoproteins - biosynthesis Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Receptors, IgG - biosynthesis Sialic Acid Binding Ig-like Lectin 3 Th1 Cells - immunology Th2 Cells - immunology |
| title | T-cell co-stimulatory molecules are upregulated on intestinal macrophages from inflammatory bowel disease mucosa |
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