Synapsin I Identified as a Novel Brain-Specific Autoantigen
BackgroundWe report the identification and characterization of a novel 74-kd brain-specific autoantigen that is reactive with serum from a patient with discoid lupus erythematosus and chronic lymphocytic leukemia.MethodsWe determined the molecular weight, tissue distribution and subcellular distribu...
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| Veröffentlicht in: | Journal of investigative medicine 2001-05, Vol.49 (3), p.276-283 |
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| creator | Gitlits, Veronika M. Sentry, John W. Matthew, Mary L.S.M. Smith, Ian A. Toh, Ban Hock |
| description | BackgroundWe report the identification and characterization of a novel 74-kd brain-specific autoantigen that is reactive with serum from a patient with discoid lupus erythematosus and chronic lymphocytic leukemia.MethodsWe determined the molecular weight, tissue distribution and subcellular distribution of the autoantigen and obtained limited amino acid sequence after purification by ion-exchange chromatography and trypsin digestion.ResultsWe identified the 74-kd autoantigen as synapsin I on the basis of the following observations. First, the autoantigen has properties consistent with synapsin I: molecular weight of ≈74 kd, brain-specific distribution, presence in cytosol and on synaptosomes, and association with taxol-stabilized microtubules. Second, limited amino acid sequence determination after trypsin digestion of the autoantigen shows identity with synapsin I. Third, the autoimmune serum immunoblots fusion proteins that incorporate rat synapsin Ia. The autoantibodies reactive to synapsin Ia are of immunoglobulin (Ig) G and IgM class.ConclusionsThis is the first report of autoantibodies that are reactive to synapsin Ia. Autoantibodies that are reactive to synapsin Ia are not restricted to discoid lupus erythematosus patients, because we found identical reactivity in two of 18 sera from dsDNA-positive systemic lupus erythematosus patients and in two of 14 rheumatoid factor-positive sera. Whether autoantibodies to synapsin I are associated with neuropsychiatric manifestations is currently unknown. |
| doi_str_mv | 10.2310/6650.2001.33973 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70835955</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sage_id>10.2310_6650.2001.33973</sage_id><sourcerecordid>70835955</sourcerecordid><originalsourceid>FETCH-LOGICAL-b449t-7ee2b46125d5034612076ae53b8bbc65888ee0b1c215ae25e0132b8e31834d13</originalsourceid><addsrcrecordid>eNqFkM9LwzAYhoMobk7P3qQoeBC75UuaNMXTHP4YDD1s95C030ZH185mFfbfm7qBIgxPeSFP3u_LQ8gl0D7jQAdSCp8ohT7nScyPSBdiqkLFZHzsM1UQCqGSDjlzbkkpkyJhp6QDwAUDJbvkYbotzdrlZTAOxhmWm3yeYxYYF5jgrfrEInisTV6G0zWm_ioNhs2mMh5bYHlOTuamcHixP3tk9vw0G72Gk_eX8Wg4CW0UJZswRmQ2ksBEJihvA42lQcGtsjaVQimFSC2kDIRBJpACZ1YhB8WjDHiP3O5q13X10aDb6FXuUiwKU2LVOO0_zEUihAev_4DLqqlLv5pmnEMMMmnbbg5BECuZeDFSemqwo9K6cq7GuV7X-crUWw1Ut-Z1a1635vW3ef_iat_b2BVmP_xetQfud4AzC_w19GDf3Q63q-W_w78ACAuTJw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1786935266</pqid></control><display><type>article</type><title>Synapsin I Identified as a Novel Brain-Specific Autoantigen</title><source>MEDLINE</source><source>SAGE Complete</source><creator>Gitlits, Veronika M. ; Sentry, John W. ; Matthew, Mary L.S.M. ; Smith, Ian A. ; Toh, Ban Hock</creator><creatorcontrib>Gitlits, Veronika M. ; Sentry, John W. ; Matthew, Mary L.S.M. ; Smith, Ian A. ; Toh, Ban Hock</creatorcontrib><description>BackgroundWe report the identification and characterization of a novel 74-kd brain-specific autoantigen that is reactive with serum from a patient with discoid lupus erythematosus and chronic lymphocytic leukemia.MethodsWe determined the molecular weight, tissue distribution and subcellular distribution of the autoantigen and obtained limited amino acid sequence after purification by ion-exchange chromatography and trypsin digestion.ResultsWe identified the 74-kd autoantigen as synapsin I on the basis of the following observations. First, the autoantigen has properties consistent with synapsin I: molecular weight of ≈74 kd, brain-specific distribution, presence in cytosol and on synaptosomes, and association with taxol-stabilized microtubules. Second, limited amino acid sequence determination after trypsin digestion of the autoantigen shows identity with synapsin I. Third, the autoimmune serum immunoblots fusion proteins that incorporate rat synapsin Ia. The autoantibodies reactive to synapsin Ia are of immunoglobulin (Ig) G and IgM class.ConclusionsThis is the first report of autoantibodies that are reactive to synapsin Ia. Autoantibodies that are reactive to synapsin Ia are not restricted to discoid lupus erythematosus patients, because we found identical reactivity in two of 18 sera from dsDNA-positive systemic lupus erythematosus patients and in two of 14 rheumatoid factor-positive sera. Whether autoantibodies to synapsin I are associated with neuropsychiatric manifestations is currently unknown.</description><identifier>ISSN: 1081-5589</identifier><identifier>EISSN: 1708-8267</identifier><identifier>DOI: 10.2310/6650.2001.33973</identifier><identifier>PMID: 11352186</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Animals ; Autoantibodies - immunology ; Autoantigens - immunology ; Autoantigens - isolation & purification ; Brain - immunology ; Brain Chemistry ; Cattle ; Chromatography, Ion Exchange ; Electrophoresis, Polyacrylamide Gel ; HeLa Cells ; Humans ; Immunoblotting ; Leukemia, Lymphocytic, Chronic, B-Cell - immunology ; Lupus Erythematosus, Discoid - immunology ; Molecular Weight ; Rats ; Rats, Sprague-Dawley ; Recombinant Fusion Proteins - immunology ; Recombinant Fusion Proteins - isolation & purification ; Sodium Dodecyl Sulfate ; Synapsins - immunology ; Synapsins - isolation & purification</subject><ispartof>Journal of investigative medicine, 2001-05, Vol.49 (3), p.276-283</ispartof><rights>2015 American Federation for Medical Research, Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions</rights><rights>2001 American Federation for Medical Research</rights><rights>Copyright: 2015 (c) 2015 American Federation for Medical Research, Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions</rights><rights>Copyright SLACK INCORPORATED May 2001</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b449t-7ee2b46125d5034612076ae53b8bbc65888ee0b1c215ae25e0132b8e31834d13</citedby><cites>FETCH-LOGICAL-b449t-7ee2b46125d5034612076ae53b8bbc65888ee0b1c215ae25e0132b8e31834d13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.2310/6650.2001.33973$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.2310/6650.2001.33973$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,776,780,21798,27901,27902,43597,43598</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11352186$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gitlits, Veronika M.</creatorcontrib><creatorcontrib>Sentry, John W.</creatorcontrib><creatorcontrib>Matthew, Mary L.S.M.</creatorcontrib><creatorcontrib>Smith, Ian A.</creatorcontrib><creatorcontrib>Toh, Ban Hock</creatorcontrib><title>Synapsin I Identified as a Novel Brain-Specific Autoantigen</title><title>Journal of investigative medicine</title><addtitle>J Investig Med</addtitle><description>BackgroundWe report the identification and characterization of a novel 74-kd brain-specific autoantigen that is reactive with serum from a patient with discoid lupus erythematosus and chronic lymphocytic leukemia.MethodsWe determined the molecular weight, tissue distribution and subcellular distribution of the autoantigen and obtained limited amino acid sequence after purification by ion-exchange chromatography and trypsin digestion.ResultsWe identified the 74-kd autoantigen as synapsin I on the basis of the following observations. First, the autoantigen has properties consistent with synapsin I: molecular weight of ≈74 kd, brain-specific distribution, presence in cytosol and on synaptosomes, and association with taxol-stabilized microtubules. Second, limited amino acid sequence determination after trypsin digestion of the autoantigen shows identity with synapsin I. Third, the autoimmune serum immunoblots fusion proteins that incorporate rat synapsin Ia. The autoantibodies reactive to synapsin Ia are of immunoglobulin (Ig) G and IgM class.ConclusionsThis is the first report of autoantibodies that are reactive to synapsin Ia. Autoantibodies that are reactive to synapsin Ia are not restricted to discoid lupus erythematosus patients, because we found identical reactivity in two of 18 sera from dsDNA-positive systemic lupus erythematosus patients and in two of 14 rheumatoid factor-positive sera. Whether autoantibodies to synapsin I are associated with neuropsychiatric manifestations is currently unknown.</description><subject>Animals</subject><subject>Autoantibodies - immunology</subject><subject>Autoantigens - immunology</subject><subject>Autoantigens - isolation & purification</subject><subject>Brain - immunology</subject><subject>Brain Chemistry</subject><subject>Cattle</subject><subject>Chromatography, Ion Exchange</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Immunoblotting</subject><subject>Leukemia, Lymphocytic, Chronic, B-Cell - immunology</subject><subject>Lupus Erythematosus, Discoid - immunology</subject><subject>Molecular Weight</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Recombinant Fusion Proteins - immunology</subject><subject>Recombinant Fusion Proteins - isolation & purification</subject><subject>Sodium Dodecyl Sulfate</subject><subject>Synapsins - immunology</subject><subject>Synapsins - isolation & purification</subject><issn>1081-5589</issn><issn>1708-8267</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkM9LwzAYhoMobk7P3qQoeBC75UuaNMXTHP4YDD1s95C030ZH185mFfbfm7qBIgxPeSFP3u_LQ8gl0D7jQAdSCp8ohT7nScyPSBdiqkLFZHzsM1UQCqGSDjlzbkkpkyJhp6QDwAUDJbvkYbotzdrlZTAOxhmWm3yeYxYYF5jgrfrEInisTV6G0zWm_ioNhs2mMh5bYHlOTuamcHixP3tk9vw0G72Gk_eX8Wg4CW0UJZswRmQ2ksBEJihvA42lQcGtsjaVQimFSC2kDIRBJpACZ1YhB8WjDHiP3O5q13X10aDb6FXuUiwKU2LVOO0_zEUihAev_4DLqqlLv5pmnEMMMmnbbg5BECuZeDFSemqwo9K6cq7GuV7X-crUWw1Ut-Z1a1635vW3ef_iat_b2BVmP_xetQfud4AzC_w19GDf3Q63q-W_w78ACAuTJw</recordid><startdate>20010501</startdate><enddate>20010501</enddate><creator>Gitlits, Veronika M.</creator><creator>Sentry, John W.</creator><creator>Matthew, Mary L.S.M.</creator><creator>Smith, Ian A.</creator><creator>Toh, Ban Hock</creator><general>SAGE Publications</general><general>Sage Publications Ltd</general><general>SLACK INCORPORATED</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>0-V</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AM</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ALSLI</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGRYB</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K7.</scope><scope>K9.</scope><scope>M0O</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20010501</creationdate><title>Synapsin I Identified as a Novel Brain-Specific Autoantigen</title><author>Gitlits, Veronika M. ; Sentry, John W. ; Matthew, Mary L.S.M. ; Smith, Ian A. ; Toh, Ban Hock</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b449t-7ee2b46125d5034612076ae53b8bbc65888ee0b1c215ae25e0132b8e31834d13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Autoantibodies - immunology</topic><topic>Autoantigens - immunology</topic><topic>Autoantigens - isolation & purification</topic><topic>Brain - immunology</topic><topic>Brain Chemistry</topic><topic>Cattle</topic><topic>Chromatography, Ion Exchange</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Immunoblotting</topic><topic>Leukemia, Lymphocytic, Chronic, B-Cell - immunology</topic><topic>Lupus Erythematosus, Discoid - immunology</topic><topic>Molecular Weight</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Recombinant Fusion Proteins - immunology</topic><topic>Recombinant Fusion Proteins - isolation & purification</topic><topic>Sodium Dodecyl Sulfate</topic><topic>Synapsins - immunology</topic><topic>Synapsins - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gitlits, Veronika M.</creatorcontrib><creatorcontrib>Sentry, John W.</creatorcontrib><creatorcontrib>Matthew, Mary L.S.M.</creatorcontrib><creatorcontrib>Smith, Ian A.</creatorcontrib><creatorcontrib>Toh, Ban Hock</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Social Sciences Premium Collection</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Criminal Justice Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Social Science Premium Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Criminology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Criminal Justice (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Criminal Justice</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of investigative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gitlits, Veronika M.</au><au>Sentry, John W.</au><au>Matthew, Mary L.S.M.</au><au>Smith, Ian A.</au><au>Toh, Ban Hock</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synapsin I Identified as a Novel Brain-Specific Autoantigen</atitle><jtitle>Journal of investigative medicine</jtitle><addtitle>J Investig Med</addtitle><date>2001-05-01</date><risdate>2001</risdate><volume>49</volume><issue>3</issue><spage>276</spage><epage>283</epage><pages>276-283</pages><issn>1081-5589</issn><eissn>1708-8267</eissn><abstract>BackgroundWe report the identification and characterization of a novel 74-kd brain-specific autoantigen that is reactive with serum from a patient with discoid lupus erythematosus and chronic lymphocytic leukemia.MethodsWe determined the molecular weight, tissue distribution and subcellular distribution of the autoantigen and obtained limited amino acid sequence after purification by ion-exchange chromatography and trypsin digestion.ResultsWe identified the 74-kd autoantigen as synapsin I on the basis of the following observations. First, the autoantigen has properties consistent with synapsin I: molecular weight of ≈74 kd, brain-specific distribution, presence in cytosol and on synaptosomes, and association with taxol-stabilized microtubules. Second, limited amino acid sequence determination after trypsin digestion of the autoantigen shows identity with synapsin I. Third, the autoimmune serum immunoblots fusion proteins that incorporate rat synapsin Ia. The autoantibodies reactive to synapsin Ia are of immunoglobulin (Ig) G and IgM class.ConclusionsThis is the first report of autoantibodies that are reactive to synapsin Ia. Autoantibodies that are reactive to synapsin Ia are not restricted to discoid lupus erythematosus patients, because we found identical reactivity in two of 18 sera from dsDNA-positive systemic lupus erythematosus patients and in two of 14 rheumatoid factor-positive sera. Whether autoantibodies to synapsin I are associated with neuropsychiatric manifestations is currently unknown.</abstract><cop>Los Angeles, CA</cop><pub>SAGE Publications</pub><pmid>11352186</pmid><doi>10.2310/6650.2001.33973</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Autoantibodies - immunology Autoantigens - immunology Autoantigens - isolation & purification Brain - immunology Brain Chemistry Cattle Chromatography, Ion Exchange Electrophoresis, Polyacrylamide Gel HeLa Cells Humans Immunoblotting Leukemia, Lymphocytic, Chronic, B-Cell - immunology Lupus Erythematosus, Discoid - immunology Molecular Weight Rats Rats, Sprague-Dawley Recombinant Fusion Proteins - immunology Recombinant Fusion Proteins - isolation & purification Sodium Dodecyl Sulfate Synapsins - immunology Synapsins - isolation & purification |
| title | Synapsin I Identified as a Novel Brain-Specific Autoantigen |
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