Functional Interaction of the Active Zone Proteins Munc13-1 and RIM1 in Synaptic Vesicle Priming
Synaptic neurotransmitter release is restricted to active zones, where the processes of synaptic vesicle tethering, priming to fusion competence, and Ca 2+-triggered fusion are taking place in a highly coordinated manner. We show that the active zone components Munc13-1, an essential vesicle priming...
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| Veröffentlicht in: | Neuron (Cambridge, Mass.) Mass.), 2001-04, Vol.30 (1), p.183-196 |
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| creator | Betz, Andrea Thakur, Pratima Junge, Harald J. Ashery, Uri Rhee, Jeong-Seop Scheuss, Volker Rosenmund, Christian Rettig, Jens Brose, Nils |
| description | Synaptic neurotransmitter release is restricted to active zones, where the processes of synaptic vesicle tethering, priming to fusion competence, and Ca
2+-triggered fusion are taking place in a highly coordinated manner. We show that the active zone components Munc13-1, an essential vesicle priming protein, and RIM1, a Rab3 effector with a putative role in vesicle tethering, interact functionally. Disruption of this interaction causes a loss of fusion-competent synaptic vesicles, creating a phenocopy of Munc13-1-deficient neurons. RIM1 binding and vesicle priming are mediated by two distinct structural modules of Munc13-1. The Munc13-1/RIM1 interaction may create a functional link between synaptic vesicle tethering and priming, or it may regulate the priming reaction itself, thereby determining the number of fusion-competent vesicles. |
| doi_str_mv | 10.1016/S0896-6273(01)00272-0 |
| format | Article |
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| subjects | Alternative Splicing - genetics Animals Binding Sites - genetics Cells, Cultured DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Fungal Proteins - genetics Fungal Proteins - metabolism Molecular Sequence Data Munc13-1 protein Nerve Tissue Proteins - genetics Nerve Tissue Proteins - metabolism Neurons - metabolism Neurotransmitter Agents - metabolism Presynaptic Terminals - metabolism Protein Isoforms - genetics Protein Isoforms - metabolism Protein Structure, Tertiary - genetics rab3A GTP-Binding Protein - metabolism RIM1 protein Sequence Homology, Amino Acid Synaptic Transmission - physiology Synaptic Vesicles - metabolism Two-Hybrid System Techniques Zinc Fingers - physiology |
| title | Functional Interaction of the Active Zone Proteins Munc13-1 and RIM1 in Synaptic Vesicle Priming |
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