Involvement of mi-transcription factor in expression of alpha-melanocyte-stimulating hormone receptor in cultured mast cells of mice
The microphthalmia (mi) locus encodes a member of the basic-helix-loop-helix-leucine zipper (bHLH-Zip) protein family of transcription factors (MITF). We have reported that expression of several genes was impaired in cultured mast cells (CMCs) of mi/mi mice due to a defective transactivation ability...
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| Veröffentlicht in: | The Journal of immunology (1950) 2000-01, Vol.164 (2), p.855-860 |
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| container_title | The Journal of immunology (1950) |
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| creator | Adachi, S Morii, E Kim, D k Ogihara, H Jippo, T Ito, A Lee, Y M Kitamura, Y |
| description | The microphthalmia (mi) locus encodes a member of the basic-helix-loop-helix-leucine zipper (bHLH-Zip) protein family of transcription factors (MITF). We have reported that expression of several genes was impaired in cultured mast cells (CMCs) of mi/mi mice due to a defective transactivation ability of mutant MITF (mi-MITF). We also found that mi/mi CMCs did not express a receptor (MC1R) for alpha-melanocyte-stimulating hormone. The overexpression of the wild-type (+/+) MITF but not mi-MITF normalized the expression of the MC1R in mi/mi CMCs, indicating the involvement of +-MITF in the MC1R gene expression. Next, we analyzed the promoter region of the MC1R gene by the transient cotransfection assay. The luciferase construct under the control of the MC1R promoter and the cDNA-encoding +-MITF or mi-MITF were cotransfected into NIH/3T3 fibroblasts. The cotransfection of +-MITF but not mi-MITF increased the luciferase activity. There were five CANNTG motifs recognized by bHLH-Zip-type transcription factors in the cloned promoter region. We found +-MITF bound two of five CANNTG motifs, and both motifs were essential for the transactivation of the MC1R gene by +-MITF. These results indicated that +-MITF directly transactivated the MC1R gene through these two motifs. |
| doi_str_mv | 10.4049/jimmunol.164.2.855 |
| format | Article |
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We have reported that expression of several genes was impaired in cultured mast cells (CMCs) of mi/mi mice due to a defective transactivation ability of mutant MITF (mi-MITF). We also found that mi/mi CMCs did not express a receptor (MC1R) for alpha-melanocyte-stimulating hormone. The overexpression of the wild-type (+/+) MITF but not mi-MITF normalized the expression of the MC1R in mi/mi CMCs, indicating the involvement of +-MITF in the MC1R gene expression. Next, we analyzed the promoter region of the MC1R gene by the transient cotransfection assay. The luciferase construct under the control of the MC1R promoter and the cDNA-encoding +-MITF or mi-MITF were cotransfected into NIH/3T3 fibroblasts. The cotransfection of +-MITF but not mi-MITF increased the luciferase activity. There were five CANNTG motifs recognized by bHLH-Zip-type transcription factors in the cloned promoter region. We found +-MITF bound two of five CANNTG motifs, and both motifs were essential for the transactivation of the MC1R gene by +-MITF. These results indicated that +-MITF directly transactivated the MC1R gene through these two motifs.</description><identifier>ISSN: 0022-1767</identifier><identifier>EISSN: 1550-6606</identifier><identifier>DOI: 10.4049/jimmunol.164.2.855</identifier><identifier>PMID: 10623832</identifier><language>eng</language><publisher>United States</publisher><subject>a-melanocyte-stimulating hormone receptors ; alpha-MSH - metabolism ; Animals ; Base Sequence ; Cells, Cultured ; DNA-Binding Proteins - physiology ; Female ; Gene Expression Regulation ; Helix-Loop-Helix Motifs - genetics ; Male ; Mast Cells - metabolism ; Mast Cells - pathology ; Mast Cells - physiology ; Mice ; Mice, Inbred C57BL ; Mice, Mutant Strains ; Mice, Transgenic ; Microphthalmia-Associated Transcription Factor ; Molecular Sequence Data ; Promoter Regions, Genetic - genetics ; Receptors, Corticotropin - biosynthesis ; Receptors, Corticotropin - genetics ; Receptors, Melanocortin ; Receptors, Pituitary Hormone - biosynthesis ; Transcription Factors - physiology ; Transcriptional Activation - genetics</subject><ispartof>The Journal of immunology (1950), 2000-01, Vol.164 (2), p.855-860</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c330t-d30845b518feb4ea7452b60fdaf42e97e4ed138e004074be1320f05368827d173</citedby><cites>FETCH-LOGICAL-c330t-d30845b518feb4ea7452b60fdaf42e97e4ed138e004074be1320f05368827d173</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10623832$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Adachi, S</creatorcontrib><creatorcontrib>Morii, E</creatorcontrib><creatorcontrib>Kim, D k</creatorcontrib><creatorcontrib>Ogihara, H</creatorcontrib><creatorcontrib>Jippo, T</creatorcontrib><creatorcontrib>Ito, A</creatorcontrib><creatorcontrib>Lee, Y M</creatorcontrib><creatorcontrib>Kitamura, Y</creatorcontrib><title>Involvement of mi-transcription factor in expression of alpha-melanocyte-stimulating hormone receptor in cultured mast cells of mice</title><title>The Journal of immunology (1950)</title><addtitle>J Immunol</addtitle><description>The microphthalmia (mi) locus encodes a member of the basic-helix-loop-helix-leucine zipper (bHLH-Zip) protein family of transcription factors (MITF). We have reported that expression of several genes was impaired in cultured mast cells (CMCs) of mi/mi mice due to a defective transactivation ability of mutant MITF (mi-MITF). We also found that mi/mi CMCs did not express a receptor (MC1R) for alpha-melanocyte-stimulating hormone. The overexpression of the wild-type (+/+) MITF but not mi-MITF normalized the expression of the MC1R in mi/mi CMCs, indicating the involvement of +-MITF in the MC1R gene expression. Next, we analyzed the promoter region of the MC1R gene by the transient cotransfection assay. The luciferase construct under the control of the MC1R promoter and the cDNA-encoding +-MITF or mi-MITF were cotransfected into NIH/3T3 fibroblasts. The cotransfection of +-MITF but not mi-MITF increased the luciferase activity. There were five CANNTG motifs recognized by bHLH-Zip-type transcription factors in the cloned promoter region. We found +-MITF bound two of five CANNTG motifs, and both motifs were essential for the transactivation of the MC1R gene by +-MITF. These results indicated that +-MITF directly transactivated the MC1R gene through these two motifs.</description><subject>a-melanocyte-stimulating hormone receptors</subject><subject>alpha-MSH - metabolism</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Cells, Cultured</subject><subject>DNA-Binding Proteins - physiology</subject><subject>Female</subject><subject>Gene Expression Regulation</subject><subject>Helix-Loop-Helix Motifs - genetics</subject><subject>Male</subject><subject>Mast Cells - metabolism</subject><subject>Mast Cells - pathology</subject><subject>Mast Cells - physiology</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Mutant Strains</subject><subject>Mice, Transgenic</subject><subject>Microphthalmia-Associated Transcription Factor</subject><subject>Molecular Sequence Data</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Receptors, Corticotropin - biosynthesis</subject><subject>Receptors, Corticotropin - genetics</subject><subject>Receptors, Melanocortin</subject><subject>Receptors, Pituitary Hormone - biosynthesis</subject><subject>Transcription Factors - physiology</subject><subject>Transcriptional Activation - genetics</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkTtvFDEQxy0EIkfIF6BAruj2GD93U6KIQKRINKS2vN7ZxJEfi-2NSM8Hz57ukOioRhr9H6P5EfKBwV6CvPz86GNcUw57puWe7welXpEdUwo6rUG_JjsAzjvW6_6MvKv1EQA0cPmWnDHQXAyC78ifm_SUwxNGTI3mmUbftWJTdcUvzedEZ-taLtQnir-XgrUelpvQhuXBdhGDTdk9N-xq83ENtvl0Tx9yiTkhLehwOdndGtpacKLR1kYdhlCPhQ7fkzezDRUvTvOc3F1__Xn1vbv98e3m6stt54SA1k0CBqlGxYYZR4m2l4qPGubJzpLjZY8SJyYGBJDQyxGZ4DCDEnoYeD-xXpyTT8fcpeRfK9Zmoq-HS2zCvFbTw_YTBfK_QrZVC6kOQn4UupJrLTibpfhoy7NhYA6QzF9IZoNkuNkgbaaPp_R1jDj9YzlSES9gtZKt</recordid><startdate>20000115</startdate><enddate>20000115</enddate><creator>Adachi, S</creator><creator>Morii, E</creator><creator>Kim, D k</creator><creator>Ogihara, H</creator><creator>Jippo, T</creator><creator>Ito, A</creator><creator>Lee, Y M</creator><creator>Kitamura, Y</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TM</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20000115</creationdate><title>Involvement of mi-transcription factor in expression of alpha-melanocyte-stimulating hormone receptor in cultured mast cells of mice</title><author>Adachi, S ; Morii, E ; Kim, D k ; Ogihara, H ; Jippo, T ; Ito, A ; Lee, Y M ; Kitamura, Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c330t-d30845b518feb4ea7452b60fdaf42e97e4ed138e004074be1320f05368827d173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>a-melanocyte-stimulating hormone receptors</topic><topic>alpha-MSH - metabolism</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Cells, Cultured</topic><topic>DNA-Binding Proteins - physiology</topic><topic>Female</topic><topic>Gene Expression Regulation</topic><topic>Helix-Loop-Helix Motifs - genetics</topic><topic>Male</topic><topic>Mast Cells - metabolism</topic><topic>Mast Cells - pathology</topic><topic>Mast Cells - physiology</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Mutant Strains</topic><topic>Mice, Transgenic</topic><topic>Microphthalmia-Associated Transcription Factor</topic><topic>Molecular Sequence Data</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Receptors, Corticotropin - biosynthesis</topic><topic>Receptors, Corticotropin - genetics</topic><topic>Receptors, Melanocortin</topic><topic>Receptors, Pituitary Hormone - biosynthesis</topic><topic>Transcription Factors - physiology</topic><topic>Transcriptional Activation - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Adachi, S</creatorcontrib><creatorcontrib>Morii, E</creatorcontrib><creatorcontrib>Kim, D k</creatorcontrib><creatorcontrib>Ogihara, H</creatorcontrib><creatorcontrib>Jippo, T</creatorcontrib><creatorcontrib>Ito, A</creatorcontrib><creatorcontrib>Lee, Y M</creatorcontrib><creatorcontrib>Kitamura, Y</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Adachi, S</au><au>Morii, E</au><au>Kim, D k</au><au>Ogihara, H</au><au>Jippo, T</au><au>Ito, A</au><au>Lee, Y M</au><au>Kitamura, Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Involvement of mi-transcription factor in expression of alpha-melanocyte-stimulating hormone receptor in cultured mast cells of mice</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>2000-01-15</date><risdate>2000</risdate><volume>164</volume><issue>2</issue><spage>855</spage><epage>860</epage><pages>855-860</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><abstract>The microphthalmia (mi) locus encodes a member of the basic-helix-loop-helix-leucine zipper (bHLH-Zip) protein family of transcription factors (MITF). We have reported that expression of several genes was impaired in cultured mast cells (CMCs) of mi/mi mice due to a defective transactivation ability of mutant MITF (mi-MITF). We also found that mi/mi CMCs did not express a receptor (MC1R) for alpha-melanocyte-stimulating hormone. The overexpression of the wild-type (+/+) MITF but not mi-MITF normalized the expression of the MC1R in mi/mi CMCs, indicating the involvement of +-MITF in the MC1R gene expression. Next, we analyzed the promoter region of the MC1R gene by the transient cotransfection assay. The luciferase construct under the control of the MC1R promoter and the cDNA-encoding +-MITF or mi-MITF were cotransfected into NIH/3T3 fibroblasts. The cotransfection of +-MITF but not mi-MITF increased the luciferase activity. There were five CANNTG motifs recognized by bHLH-Zip-type transcription factors in the cloned promoter region. We found +-MITF bound two of five CANNTG motifs, and both motifs were essential for the transactivation of the MC1R gene by +-MITF. These results indicated that +-MITF directly transactivated the MC1R gene through these two motifs.</abstract><cop>United States</cop><pmid>10623832</pmid><doi>10.4049/jimmunol.164.2.855</doi><tpages>6</tpages></addata></record> |
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| subjects | a-melanocyte-stimulating hormone receptors alpha-MSH - metabolism Animals Base Sequence Cells, Cultured DNA-Binding Proteins - physiology Female Gene Expression Regulation Helix-Loop-Helix Motifs - genetics Male Mast Cells - metabolism Mast Cells - pathology Mast Cells - physiology Mice Mice, Inbred C57BL Mice, Mutant Strains Mice, Transgenic Microphthalmia-Associated Transcription Factor Molecular Sequence Data Promoter Regions, Genetic - genetics Receptors, Corticotropin - biosynthesis Receptors, Corticotropin - genetics Receptors, Melanocortin Receptors, Pituitary Hormone - biosynthesis Transcription Factors - physiology Transcriptional Activation - genetics |
| title | Involvement of mi-transcription factor in expression of alpha-melanocyte-stimulating hormone receptor in cultured mast cells of mice |
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