Collagen breakdown in soft connective tissue explants is associated with the level of active gelatinase A (MMP-2) but not with collagenase
Recent data suggest that gelatinase A (matrix metalloproteinase-2, MMP-2) plays an important role in the degradation of collagen of soft connective tissues. In an attempt to investigate its participation in more detail we assessed the digestion of collagen in cultured rabbit periosteal explants and...
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| Veröffentlicht in: | Matrix biology 1999-08, Vol.18 (4), p.373-380 |
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| creator | Kerkvliet, Erica H.M Docherty, Andrew J.P Beertsen, Wouter Everts, Vincent |
| description | Recent data suggest that gelatinase A (matrix metalloproteinase-2, MMP-2) plays an important role in the degradation of collagen of soft connective tissues. In an attempt to investigate its participation in more detail we assessed the digestion of collagen in cultured rabbit periosteal explants and compared this with the level of active MMP-2 and collagenases. The data demonstrated that both collagen degradation and MMP activity increased with time. Conditioned medium obtained from explants cultured for 72 h showed that the level of active MMP-2 correlated with collagen degradation (
r=0.80, d.f.=23,
P |
| doi_str_mv | 10.1016/S0945-053X(99)00032-3 |
| format | Article |
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r=0.80, d.f.=23,
P<0.0001). Such a relationship was not found with collagenase activity (
r=−0.08, d.f.=21, NS). The possible involvement of MMP-2 in collagen degradation was investigated further by incubating explants with selective gelatinase inhibitors (CT1166, CT1399 and CT1746). In the presence of these compounds breakdown of collagen was almost completely abolished (≈80%). Finally we assessed whether periosteal fibroblasts had the capacity to degrade collagen type I that conferred resistance to collagenase activity. Breakdown of this collagen did not differ from degradation of normal collagen. Taken together, our data provide support for the view that MMP-2 plays a crucial role in collagen degradation of soft connective tissue.</description><identifier>ISSN: 0945-053X</identifier><identifier>EISSN: 1569-1802</identifier><identifier>DOI: 10.1016/S0945-053X(99)00032-3</identifier><identifier>PMID: 10517184</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Collagen - metabolism ; Collagen degradation ; Collagenases - metabolism ; Connective Tissue - enzymology ; Connective Tissue - metabolism ; Culture Techniques ; Data Interpretation, Statistical ; Hydroxyproline - metabolism ; Immunohistochemistry ; Matrix Metalloproteinase 1 - metabolism ; Matrix Metalloproteinase 13 ; Matrix Metalloproteinase 2 - metabolism ; Matrix Metalloproteinase 8 - metabolism ; Matrix metalloproteinases ; Matrix Metalloproteinases - metabolism ; MMP-inhibitors ; Periosteal explants ; Periosteum - enzymology ; Rabbits ; Skull - enzymology</subject><ispartof>Matrix biology, 1999-08, Vol.18 (4), p.373-380</ispartof><rights>1999 Journal of the International Society for Matrix Biology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c361t-577ce4e74b363d9115ea1ad55f26cbcaaf445818a7164d8e74a571a21e95d64f3</citedby><cites>FETCH-LOGICAL-c361t-577ce4e74b363d9115ea1ad55f26cbcaaf445818a7164d8e74a571a21e95d64f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0945053X99000323$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10517184$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kerkvliet, Erica H.M</creatorcontrib><creatorcontrib>Docherty, Andrew J.P</creatorcontrib><creatorcontrib>Beertsen, Wouter</creatorcontrib><creatorcontrib>Everts, Vincent</creatorcontrib><title>Collagen breakdown in soft connective tissue explants is associated with the level of active gelatinase A (MMP-2) but not with collagenase</title><title>Matrix biology</title><addtitle>Matrix Biol</addtitle><description>Recent data suggest that gelatinase A (matrix metalloproteinase-2, MMP-2) plays an important role in the degradation of collagen of soft connective tissues. In an attempt to investigate its participation in more detail we assessed the digestion of collagen in cultured rabbit periosteal explants and compared this with the level of active MMP-2 and collagenases. The data demonstrated that both collagen degradation and MMP activity increased with time. Conditioned medium obtained from explants cultured for 72 h showed that the level of active MMP-2 correlated with collagen degradation (
r=0.80, d.f.=23,
P<0.0001). Such a relationship was not found with collagenase activity (
r=−0.08, d.f.=21, NS). The possible involvement of MMP-2 in collagen degradation was investigated further by incubating explants with selective gelatinase inhibitors (CT1166, CT1399 and CT1746). In the presence of these compounds breakdown of collagen was almost completely abolished (≈80%). Finally we assessed whether periosteal fibroblasts had the capacity to degrade collagen type I that conferred resistance to collagenase activity. Breakdown of this collagen did not differ from degradation of normal collagen. Taken together, our data provide support for the view that MMP-2 plays a crucial role in collagen degradation of soft connective tissue.</description><subject>Animals</subject><subject>Collagen - metabolism</subject><subject>Collagen degradation</subject><subject>Collagenases - metabolism</subject><subject>Connective Tissue - enzymology</subject><subject>Connective Tissue - metabolism</subject><subject>Culture Techniques</subject><subject>Data Interpretation, Statistical</subject><subject>Hydroxyproline - metabolism</subject><subject>Immunohistochemistry</subject><subject>Matrix Metalloproteinase 1 - metabolism</subject><subject>Matrix Metalloproteinase 13</subject><subject>Matrix Metalloproteinase 2 - metabolism</subject><subject>Matrix Metalloproteinase 8 - metabolism</subject><subject>Matrix metalloproteinases</subject><subject>Matrix Metalloproteinases - metabolism</subject><subject>MMP-inhibitors</subject><subject>Periosteal explants</subject><subject>Periosteum - enzymology</subject><subject>Rabbits</subject><subject>Skull - enzymology</subject><issn>0945-053X</issn><issn>1569-1802</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQhS1ERbeFnwDyCbWHgB3bcXJC1QpapFYgARI3a2JPWkPWXmJnC3-BX123WaHeOM3le_Nm3iPkJWdvOOPN2y-sk6piSnw_6bpTxpioK_GErLhquoq3rH5KVv-QQ3KU0o8CSanbZ-SQM8U1b-WK_F3HcYRrDLSfEH66eBuoDzTFIVMbQ0Cb_Q5p9inNSPH3doSQE_WJQkrResjo6K3PNzTfIB1xhyONA4VFdo0jZB8gIT2jJ1dXn6v6lPZzpiHmRWX39gV5Tg4GGBO-2M9j8u3D-6_ri-ry0_nH9dllZUXDc6W0tihRy140wnWcKwQOTqmhbmxvAQYpVctb0LyRri0gKM2h5tgp18hBHJPXy97tFH_NmLLZ-GSx3BEwzslo1tZaMFlAtYB2iilNOJjt5Dcw_TGcmfsSzEMJ5j5h03XmoQQjiu7V3mDuN-geqZbUC_BuAbC8ufM4mWQ9BovOTyVw46L_j8Ud5F6Yfw</recordid><startdate>19990801</startdate><enddate>19990801</enddate><creator>Kerkvliet, Erica H.M</creator><creator>Docherty, Andrew J.P</creator><creator>Beertsen, Wouter</creator><creator>Everts, Vincent</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990801</creationdate><title>Collagen breakdown in soft connective tissue explants is associated with the level of active gelatinase A (MMP-2) but not with collagenase</title><author>Kerkvliet, Erica H.M ; Docherty, Andrew J.P ; Beertsen, Wouter ; Everts, Vincent</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c361t-577ce4e74b363d9115ea1ad55f26cbcaaf445818a7164d8e74a571a21e95d64f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Collagen - metabolism</topic><topic>Collagen degradation</topic><topic>Collagenases - metabolism</topic><topic>Connective Tissue - enzymology</topic><topic>Connective Tissue - metabolism</topic><topic>Culture Techniques</topic><topic>Data Interpretation, Statistical</topic><topic>Hydroxyproline - metabolism</topic><topic>Immunohistochemistry</topic><topic>Matrix Metalloproteinase 1 - metabolism</topic><topic>Matrix Metalloproteinase 13</topic><topic>Matrix Metalloproteinase 2 - metabolism</topic><topic>Matrix Metalloproteinase 8 - metabolism</topic><topic>Matrix metalloproteinases</topic><topic>Matrix Metalloproteinases - metabolism</topic><topic>MMP-inhibitors</topic><topic>Periosteal explants</topic><topic>Periosteum - enzymology</topic><topic>Rabbits</topic><topic>Skull - enzymology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kerkvliet, Erica H.M</creatorcontrib><creatorcontrib>Docherty, Andrew J.P</creatorcontrib><creatorcontrib>Beertsen, Wouter</creatorcontrib><creatorcontrib>Everts, Vincent</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Matrix biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kerkvliet, Erica H.M</au><au>Docherty, Andrew J.P</au><au>Beertsen, Wouter</au><au>Everts, Vincent</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Collagen breakdown in soft connective tissue explants is associated with the level of active gelatinase A (MMP-2) but not with collagenase</atitle><jtitle>Matrix biology</jtitle><addtitle>Matrix Biol</addtitle><date>1999-08-01</date><risdate>1999</risdate><volume>18</volume><issue>4</issue><spage>373</spage><epage>380</epage><pages>373-380</pages><issn>0945-053X</issn><eissn>1569-1802</eissn><abstract>Recent data suggest that gelatinase A (matrix metalloproteinase-2, MMP-2) plays an important role in the degradation of collagen of soft connective tissues. In an attempt to investigate its participation in more detail we assessed the digestion of collagen in cultured rabbit periosteal explants and compared this with the level of active MMP-2 and collagenases. The data demonstrated that both collagen degradation and MMP activity increased with time. Conditioned medium obtained from explants cultured for 72 h showed that the level of active MMP-2 correlated with collagen degradation (
r=0.80, d.f.=23,
P<0.0001). Such a relationship was not found with collagenase activity (
r=−0.08, d.f.=21, NS). The possible involvement of MMP-2 in collagen degradation was investigated further by incubating explants with selective gelatinase inhibitors (CT1166, CT1399 and CT1746). In the presence of these compounds breakdown of collagen was almost completely abolished (≈80%). Finally we assessed whether periosteal fibroblasts had the capacity to degrade collagen type I that conferred resistance to collagenase activity. Breakdown of this collagen did not differ from degradation of normal collagen. Taken together, our data provide support for the view that MMP-2 plays a crucial role in collagen degradation of soft connective tissue.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>10517184</pmid><doi>10.1016/S0945-053X(99)00032-3</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Collagen - metabolism Collagen degradation Collagenases - metabolism Connective Tissue - enzymology Connective Tissue - metabolism Culture Techniques Data Interpretation, Statistical Hydroxyproline - metabolism Immunohistochemistry Matrix Metalloproteinase 1 - metabolism Matrix Metalloproteinase 13 Matrix Metalloproteinase 2 - metabolism Matrix Metalloproteinase 8 - metabolism Matrix metalloproteinases Matrix Metalloproteinases - metabolism MMP-inhibitors Periosteal explants Periosteum - enzymology Rabbits Skull - enzymology |
| title | Collagen breakdown in soft connective tissue explants is associated with the level of active gelatinase A (MMP-2) but not with collagenase |
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