Comparison of Permeating and Nonpermeating Cryoprotectants for Mouse Sperm Cryopreservation

Mouse sperm has proven to be more difficult to cryopreserve than sperm of other mammalian species. Published reports show that only three cryoprotectant agents (CPAs), alone or combined, have been studied: glycerol and dimethyl sulfoxide (DMSO), as permeating agents, and raffinose, as a nonpermeatin...

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Veröffentlicht in:	Cryobiology 2001-02, Vol.42 (1), p.28-39
Hauptverfasser:	Sztein, J.M., Noble, K., Farley, J.S., Mobraaten, L.E.
Format:	Artikel
Sprache:	eng
Schlagworte:	adonitol Animals Biological and medical sciences Cell Survival - drug effects Cryopreservation - methods Cryoprotective Agents - pharmacology dimethyl sulfoxide Embryo Transfer Female Fertilization in Vitro formamide Fundamental and applied biological sciences. Psychology glycerol In Vitro Techniques lactose Male Mammalian male genital system mannitol Mice Mice, Inbred C57BL Morphology. Physiology Permeability Pregnancy propanediol raffinose Semen Preservation - methods Solutions Sperm Motility - drug effects Spermatozoa - drug effects sucrose Vertebrates: reproduction
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