An automatic system for the assessment of complex medium additives under cultivation conditions

Complex medium additives such as yeast extract or peptone are often used in industrial cell culture processes to prolong cell growth and/or to improve product formation. The quality of those supplements is dependent on the preparation method and can differ from lot to lot. To guarantee consistent pr...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Biotechnology and bioengineering 2001-06, Vol.73 (6), p.442-448
Hauptverfasser: Iding, K., Büntemeyer, H., Gudermann, F., Deutschmann, S. M., Kionka, C., Lehmann, J.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 448
container_issue 6
container_start_page 442
container_title Biotechnology and bioengineering
container_volume 73
creator Iding, K.
Büntemeyer, H.
Gudermann, F.
Deutschmann, S. M.
Kionka, C.
Lehmann, J.
description Complex medium additives such as yeast extract or peptone are often used in industrial cell culture processes to prolong cell growth and/or to improve product formation. The quality of those supplements is dependent on the preparation method and can differ from lot to lot. To guarantee consistent production these different lots have to be tested prior to use in fermentation processes. Because a detailed qualitative and quantitative analysis of all components of such a complex mixture is a very difficult task, another assessment method has to be chosen. The best way to evaluate the effect of such supplements is to monitor cell activity during real cultivation conditions with and without the added supplement lot. A bioreactor‐based test system has been developed to determine the oxygen requirement of the cells as a response to the addition of a supplement to be tested under standardized conditions. Investigations were performed with a mouse‐mouse hybridoma cell line and yeast extracts as an example for complex medium additives. The results showed differences in the impact between different extract lots and between different concentrations of an extract. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 73: 442–448, 2001.
doi_str_mv 10.1002/bit.1078
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70826986</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>70826986</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4158-98d4a41d13ad384b1092f464203ca4c1af90f9245fb779e89812911b601082f33</originalsourceid><addsrcrecordid>eNqFkU1rFTEUhoMo9loFf4FkIeJmNF-Tj2VbtK0UBam4DJlMgtGZyTVnRnv_vRnuoG7EbJLDeXgP5wlCTyl5RQlhr7s014fS99COEqMawgy5j3aEENnw1rAT9Ajgay2VlvIhOqGUi3r0DtmzCbtlzqObk8dwgDmMOOaC5y8BO4AAMIZpxjlin8f9EO7wGPq0jNj1fZrTjwB4mfpQsF-GWtaYPFV0Wpt5gsfoQXQDhCfbfYo-vX1ze3HV3Hy4vL44u2m8oK1ujO6FE7Sn3PVci65uwaKQghHunfDURUOiYaKNnVImaKMpM5R2klCiWeT8FL045u5L_r4EmO2YwIdhcFPIC1hVMWm0_C9INRVGqhV8eQR9yQAlRLsvaXTlYCmxq3VbrdvVekWfbZlLV-38ATfNFXi-AQ68G2Jxk0_wV6BqW70u0Ryxn2kIh3_Os-fXt9vcjU_12-5-8658s1Jx1drP7y-t0e_M-ccrYRn_BZaapsU</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>18149676</pqid></control><display><type>article</type><title>An automatic system for the assessment of complex medium additives under cultivation conditions</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Iding, K. ; Büntemeyer, H. ; Gudermann, F. ; Deutschmann, S. M. ; Kionka, C. ; Lehmann, J.</creator><creatorcontrib>Iding, K. ; Büntemeyer, H. ; Gudermann, F. ; Deutschmann, S. M. ; Kionka, C. ; Lehmann, J.</creatorcontrib><description>Complex medium additives such as yeast extract or peptone are often used in industrial cell culture processes to prolong cell growth and/or to improve product formation. The quality of those supplements is dependent on the preparation method and can differ from lot to lot. To guarantee consistent production these different lots have to be tested prior to use in fermentation processes. Because a detailed qualitative and quantitative analysis of all components of such a complex mixture is a very difficult task, another assessment method has to be chosen. The best way to evaluate the effect of such supplements is to monitor cell activity during real cultivation conditions with and without the added supplement lot. A bioreactor‐based test system has been developed to determine the oxygen requirement of the cells as a response to the addition of a supplement to be tested under standardized conditions. Investigations were performed with a mouse‐mouse hybridoma cell line and yeast extracts as an example for complex medium additives. The results showed differences in the impact between different extract lots and between different concentrations of an extract. © 2001 John Wiley &amp; Sons, Inc. Biotechnol Bioeng 73: 442–448, 2001.</description><identifier>ISSN: 0006-3592</identifier><identifier>EISSN: 1097-0290</identifier><identifier>DOI: 10.1002/bit.1078</identifier><identifier>PMID: 11344448</identifier><identifier>CODEN: BIBIAU</identifier><language>eng</language><publisher>New York: John Wiley &amp; Sons, Inc</publisher><subject>Animal cells ; Animals ; assessment method ; Automation ; Biological and medical sciences ; bioreactor ; Bioreactors ; Biotechnology ; Cell Culture Techniques - methods ; Culture Media ; Eukaryotic cell cultures ; Fundamental and applied biological sciences. Psychology ; Hybridomas ; Methods. Procedures. Technologies ; Mice ; Miscellaneous ; oxygen ; yeast extract</subject><ispartof>Biotechnology and bioengineering, 2001-06, Vol.73 (6), p.442-448</ispartof><rights>Copyright © 2001 John Wiley &amp; Sons, Inc.</rights><rights>2001 INIST-CNRS</rights><rights>Copyright 2001 John Wiley &amp; Sons, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4158-98d4a41d13ad384b1092f464203ca4c1af90f9245fb779e89812911b601082f33</citedby><cites>FETCH-LOGICAL-c4158-98d4a41d13ad384b1092f464203ca4c1af90f9245fb779e89812911b601082f33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbit.1078$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbit.1078$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=1075583$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11344448$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Iding, K.</creatorcontrib><creatorcontrib>Büntemeyer, H.</creatorcontrib><creatorcontrib>Gudermann, F.</creatorcontrib><creatorcontrib>Deutschmann, S. M.</creatorcontrib><creatorcontrib>Kionka, C.</creatorcontrib><creatorcontrib>Lehmann, J.</creatorcontrib><title>An automatic system for the assessment of complex medium additives under cultivation conditions</title><title>Biotechnology and bioengineering</title><addtitle>Biotechnol. Bioeng</addtitle><description>Complex medium additives such as yeast extract or peptone are often used in industrial cell culture processes to prolong cell growth and/or to improve product formation. The quality of those supplements is dependent on the preparation method and can differ from lot to lot. To guarantee consistent production these different lots have to be tested prior to use in fermentation processes. Because a detailed qualitative and quantitative analysis of all components of such a complex mixture is a very difficult task, another assessment method has to be chosen. The best way to evaluate the effect of such supplements is to monitor cell activity during real cultivation conditions with and without the added supplement lot. A bioreactor‐based test system has been developed to determine the oxygen requirement of the cells as a response to the addition of a supplement to be tested under standardized conditions. Investigations were performed with a mouse‐mouse hybridoma cell line and yeast extracts as an example for complex medium additives. The results showed differences in the impact between different extract lots and between different concentrations of an extract. © 2001 John Wiley &amp; Sons, Inc. Biotechnol Bioeng 73: 442–448, 2001.</description><subject>Animal cells</subject><subject>Animals</subject><subject>assessment method</subject><subject>Automation</subject><subject>Biological and medical sciences</subject><subject>bioreactor</subject><subject>Bioreactors</subject><subject>Biotechnology</subject><subject>Cell Culture Techniques - methods</subject><subject>Culture Media</subject><subject>Eukaryotic cell cultures</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hybridomas</subject><subject>Methods. Procedures. Technologies</subject><subject>Mice</subject><subject>Miscellaneous</subject><subject>oxygen</subject><subject>yeast extract</subject><issn>0006-3592</issn><issn>1097-0290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1rFTEUhoMo9loFf4FkIeJmNF-Tj2VbtK0UBam4DJlMgtGZyTVnRnv_vRnuoG7EbJLDeXgP5wlCTyl5RQlhr7s014fS99COEqMawgy5j3aEENnw1rAT9Ajgay2VlvIhOqGUi3r0DtmzCbtlzqObk8dwgDmMOOaC5y8BO4AAMIZpxjlin8f9EO7wGPq0jNj1fZrTjwB4mfpQsF-GWtaYPFV0Wpt5gsfoQXQDhCfbfYo-vX1ze3HV3Hy4vL44u2m8oK1ujO6FE7Sn3PVci65uwaKQghHunfDURUOiYaKNnVImaKMpM5R2klCiWeT8FL045u5L_r4EmO2YwIdhcFPIC1hVMWm0_C9INRVGqhV8eQR9yQAlRLsvaXTlYCmxq3VbrdvVekWfbZlLV-38ATfNFXi-AQ68G2Jxk0_wV6BqW70u0Ryxn2kIh3_Os-fXt9vcjU_12-5-8658s1Jx1drP7y-t0e_M-ccrYRn_BZaapsU</recordid><startdate>20010620</startdate><enddate>20010620</enddate><creator>Iding, K.</creator><creator>Büntemeyer, H.</creator><creator>Gudermann, F.</creator><creator>Deutschmann, S. M.</creator><creator>Kionka, C.</creator><creator>Lehmann, J.</creator><general>John Wiley &amp; Sons, Inc</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20010620</creationdate><title>An automatic system for the assessment of complex medium additives under cultivation conditions</title><author>Iding, K. ; Büntemeyer, H. ; Gudermann, F. ; Deutschmann, S. M. ; Kionka, C. ; Lehmann, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4158-98d4a41d13ad384b1092f464203ca4c1af90f9245fb779e89812911b601082f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animal cells</topic><topic>Animals</topic><topic>assessment method</topic><topic>Automation</topic><topic>Biological and medical sciences</topic><topic>bioreactor</topic><topic>Bioreactors</topic><topic>Biotechnology</topic><topic>Cell Culture Techniques - methods</topic><topic>Culture Media</topic><topic>Eukaryotic cell cultures</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hybridomas</topic><topic>Methods. Procedures. Technologies</topic><topic>Mice</topic><topic>Miscellaneous</topic><topic>oxygen</topic><topic>yeast extract</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Iding, K.</creatorcontrib><creatorcontrib>Büntemeyer, H.</creatorcontrib><creatorcontrib>Gudermann, F.</creatorcontrib><creatorcontrib>Deutschmann, S. M.</creatorcontrib><creatorcontrib>Kionka, C.</creatorcontrib><creatorcontrib>Lehmann, J.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biotechnology and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Iding, K.</au><au>Büntemeyer, H.</au><au>Gudermann, F.</au><au>Deutschmann, S. M.</au><au>Kionka, C.</au><au>Lehmann, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An automatic system for the assessment of complex medium additives under cultivation conditions</atitle><jtitle>Biotechnology and bioengineering</jtitle><addtitle>Biotechnol. Bioeng</addtitle><date>2001-06-20</date><risdate>2001</risdate><volume>73</volume><issue>6</issue><spage>442</spage><epage>448</epage><pages>442-448</pages><issn>0006-3592</issn><eissn>1097-0290</eissn><coden>BIBIAU</coden><abstract>Complex medium additives such as yeast extract or peptone are often used in industrial cell culture processes to prolong cell growth and/or to improve product formation. The quality of those supplements is dependent on the preparation method and can differ from lot to lot. To guarantee consistent production these different lots have to be tested prior to use in fermentation processes. Because a detailed qualitative and quantitative analysis of all components of such a complex mixture is a very difficult task, another assessment method has to be chosen. The best way to evaluate the effect of such supplements is to monitor cell activity during real cultivation conditions with and without the added supplement lot. A bioreactor‐based test system has been developed to determine the oxygen requirement of the cells as a response to the addition of a supplement to be tested under standardized conditions. Investigations were performed with a mouse‐mouse hybridoma cell line and yeast extracts as an example for complex medium additives. The results showed differences in the impact between different extract lots and between different concentrations of an extract. © 2001 John Wiley &amp; Sons, Inc. Biotechnol Bioeng 73: 442–448, 2001.</abstract><cop>New York</cop><pub>John Wiley &amp; Sons, Inc</pub><pmid>11344448</pmid><doi>10.1002/bit.1078</doi><tpages>7</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0006-3592
ispartof Biotechnology and bioengineering, 2001-06, Vol.73 (6), p.442-448
issn 0006-3592
1097-0290
language eng
recordid cdi_proquest_miscellaneous_70826986
source MEDLINE; Wiley Online Library Journals Frontfile Complete
subjects Animal cells
Animals
assessment method
Automation
Biological and medical sciences
bioreactor
Bioreactors
Biotechnology
Cell Culture Techniques - methods
Culture Media
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Hybridomas
Methods. Procedures. Technologies
Mice
Miscellaneous
oxygen
yeast extract
title An automatic system for the assessment of complex medium additives under cultivation conditions
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-26T19%3A28%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=An%20automatic%20system%20for%20the%20assessment%20of%20complex%20medium%20additives%20under%20cultivation%20conditions&rft.jtitle=Biotechnology%20and%20bioengineering&rft.au=Iding,%20K.&rft.date=2001-06-20&rft.volume=73&rft.issue=6&rft.spage=442&rft.epage=448&rft.pages=442-448&rft.issn=0006-3592&rft.eissn=1097-0290&rft.coden=BIBIAU&rft_id=info:doi/10.1002/bit.1078&rft_dat=%3Cproquest_cross%3E70826986%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=18149676&rft_id=info:pmid/11344448&rfr_iscdi=true