An automatic system for the assessment of complex medium additives under cultivation conditions
Complex medium additives such as yeast extract or peptone are often used in industrial cell culture processes to prolong cell growth and/or to improve product formation. The quality of those supplements is dependent on the preparation method and can differ from lot to lot. To guarantee consistent pr...
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Veröffentlicht in: | Biotechnology and bioengineering 2001-06, Vol.73 (6), p.442-448 |
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creator | Iding, K. Büntemeyer, H. Gudermann, F. Deutschmann, S. M. Kionka, C. Lehmann, J. |
description | Complex medium additives such as yeast extract or peptone are often used in industrial cell culture processes to prolong cell growth and/or to improve product formation. The quality of those supplements is dependent on the preparation method and can differ from lot to lot. To guarantee consistent production these different lots have to be tested prior to use in fermentation processes. Because a detailed qualitative and quantitative analysis of all components of such a complex mixture is a very difficult task, another assessment method has to be chosen. The best way to evaluate the effect of such supplements is to monitor cell activity during real cultivation conditions with and without the added supplement lot. A bioreactor‐based test system has been developed to determine the oxygen requirement of the cells as a response to the addition of a supplement to be tested under standardized conditions.
Investigations were performed with a mouse‐mouse hybridoma cell line and yeast extracts as an example for complex medium additives. The results showed differences in the impact between different extract lots and between different concentrations of an extract. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 73: 442–448, 2001. |
doi_str_mv | 10.1002/bit.1078 |
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Investigations were performed with a mouse‐mouse hybridoma cell line and yeast extracts as an example for complex medium additives. The results showed differences in the impact between different extract lots and between different concentrations of an extract. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 73: 442–448, 2001.</description><identifier>ISSN: 0006-3592</identifier><identifier>EISSN: 1097-0290</identifier><identifier>DOI: 10.1002/bit.1078</identifier><identifier>PMID: 11344448</identifier><identifier>CODEN: BIBIAU</identifier><language>eng</language><publisher>New York: John Wiley & Sons, Inc</publisher><subject>Animal cells ; Animals ; assessment method ; Automation ; Biological and medical sciences ; bioreactor ; Bioreactors ; Biotechnology ; Cell Culture Techniques - methods ; Culture Media ; Eukaryotic cell cultures ; Fundamental and applied biological sciences. Psychology ; Hybridomas ; Methods. Procedures. Technologies ; Mice ; Miscellaneous ; oxygen ; yeast extract</subject><ispartof>Biotechnology and bioengineering, 2001-06, Vol.73 (6), p.442-448</ispartof><rights>Copyright © 2001 John Wiley & Sons, Inc.</rights><rights>2001 INIST-CNRS</rights><rights>Copyright 2001 John Wiley & Sons, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4158-98d4a41d13ad384b1092f464203ca4c1af90f9245fb779e89812911b601082f33</citedby><cites>FETCH-LOGICAL-c4158-98d4a41d13ad384b1092f464203ca4c1af90f9245fb779e89812911b601082f33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbit.1078$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbit.1078$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1075583$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11344448$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Iding, K.</creatorcontrib><creatorcontrib>Büntemeyer, H.</creatorcontrib><creatorcontrib>Gudermann, F.</creatorcontrib><creatorcontrib>Deutschmann, S. M.</creatorcontrib><creatorcontrib>Kionka, C.</creatorcontrib><creatorcontrib>Lehmann, J.</creatorcontrib><title>An automatic system for the assessment of complex medium additives under cultivation conditions</title><title>Biotechnology and bioengineering</title><addtitle>Biotechnol. Bioeng</addtitle><description>Complex medium additives such as yeast extract or peptone are often used in industrial cell culture processes to prolong cell growth and/or to improve product formation. The quality of those supplements is dependent on the preparation method and can differ from lot to lot. To guarantee consistent production these different lots have to be tested prior to use in fermentation processes. Because a detailed qualitative and quantitative analysis of all components of such a complex mixture is a very difficult task, another assessment method has to be chosen. The best way to evaluate the effect of such supplements is to monitor cell activity during real cultivation conditions with and without the added supplement lot. A bioreactor‐based test system has been developed to determine the oxygen requirement of the cells as a response to the addition of a supplement to be tested under standardized conditions.
Investigations were performed with a mouse‐mouse hybridoma cell line and yeast extracts as an example for complex medium additives. The results showed differences in the impact between different extract lots and between different concentrations of an extract. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 73: 442–448, 2001.</description><subject>Animal cells</subject><subject>Animals</subject><subject>assessment method</subject><subject>Automation</subject><subject>Biological and medical sciences</subject><subject>bioreactor</subject><subject>Bioreactors</subject><subject>Biotechnology</subject><subject>Cell Culture Techniques - methods</subject><subject>Culture Media</subject><subject>Eukaryotic cell cultures</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hybridomas</subject><subject>Methods. Procedures. 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Because a detailed qualitative and quantitative analysis of all components of such a complex mixture is a very difficult task, another assessment method has to be chosen. The best way to evaluate the effect of such supplements is to monitor cell activity during real cultivation conditions with and without the added supplement lot. A bioreactor‐based test system has been developed to determine the oxygen requirement of the cells as a response to the addition of a supplement to be tested under standardized conditions.
Investigations were performed with a mouse‐mouse hybridoma cell line and yeast extracts as an example for complex medium additives. The results showed differences in the impact between different extract lots and between different concentrations of an extract. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 73: 442–448, 2001.</abstract><cop>New York</cop><pub>John Wiley & Sons, Inc</pub><pmid>11344448</pmid><doi>10.1002/bit.1078</doi><tpages>7</tpages></addata></record> |
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subjects | Animal cells Animals assessment method Automation Biological and medical sciences bioreactor Bioreactors Biotechnology Cell Culture Techniques - methods Culture Media Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Hybridomas Methods. Procedures. Technologies Mice Miscellaneous oxygen yeast extract |
title | An automatic system for the assessment of complex medium additives under cultivation conditions |
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