Detection of apoptosis during orthodontic tooth movement in rats
Experimental evidence suggests that cellular changes and tissue turnover occurs in the periapical tissue during tooth movement. The possible role of apoptosis in tooth movement has never been investigated. The purpose of this study was to show whether apoptosis plays any role in orthodontic tooth mo...
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| Veröffentlicht in: | American journal of orthodontics and dentofacial orthopedics 2001-05, Vol.119 (5), p.516-521 |
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| container_title | American journal of orthodontics and dentofacial orthopedics |
| container_volume | 119 |
| creator | Rana, Mohammed Waheed-uz-Zaman Pothisiri, Vighanda Killiany, Dennis Michael Xu, Xiao Ming |
| description | Experimental evidence suggests that cellular changes and tissue turnover occurs in the periapical tissue during tooth movement. The possible role of apoptosis in tooth movement has never been investigated. The purpose of this study was to show whether apoptosis plays any role in orthodontic tooth movement. A sample of 21 adult male Sprague-Dawley rats was divided into 4 groups. Orthodontic appliances were attached to the rats for various times—to group I (n = 6) for 3 days, to group II (n = 6) for 1 week, and to group III (n = 6) for 2 weeks. Rats in group IV (n = 3) had appliances but no force and served as the control group. At the end of the observation period, the animals were killed and tissue blocks of involved teeth were processed and prepared for TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) reaction. TUNEL-positive cells from selected areas on each slide were counted and compared by means of an analysis of variance. Group I demonstrated a significantly higher (P |
| doi_str_mv | 10.1067/mod.2001.113654 |
| format | Article |
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The possible role of apoptosis in tooth movement has never been investigated. The purpose of this study was to show whether apoptosis plays any role in orthodontic tooth movement. A sample of 21 adult male Sprague-Dawley rats was divided into 4 groups. Orthodontic appliances were attached to the rats for various times—to group I (n = 6) for 3 days, to group II (n = 6) for 1 week, and to group III (n = 6) for 2 weeks. Rats in group IV (n = 3) had appliances but no force and served as the control group. At the end of the observation period, the animals were killed and tissue blocks of involved teeth were processed and prepared for TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) reaction. TUNEL-positive cells from selected areas on each slide were counted and compared by means of an analysis of variance. Group I demonstrated a significantly higher (P <.05) number of positive cells. This suggests that the maximum apoptosis occurs approximately 3 days after the application of the appliance. (Am J Orthod Dentofacial Orthop 2001;119:516-21)</description><identifier>ISSN: 0889-5406</identifier><identifier>EISSN: 1097-6752</identifier><identifier>DOI: 10.1067/mod.2001.113654</identifier><identifier>PMID: 11343024</identifier><language>eng</language><publisher>United States: Mosby, Inc</publisher><subject>Alveolar Bone Loss - etiology ; Alveolar Bone Loss - pathology ; Analysis of Variance ; Animals ; Apoptosis ; Dental Pulp - pathology ; Dental Stress Analysis ; Dentistry ; In Situ Nick-End Labeling ; Male ; Maxilla ; Molar - pathology ; Orthodontic Appliances - adverse effects ; Rats ; Rats, Sprague-Dawley ; Statistics, Nonparametric ; Time Factors ; Tooth Movement Techniques - adverse effects</subject><ispartof>American journal of orthodontics and dentofacial orthopedics, 2001-05, Vol.119 (5), p.516-521</ispartof><rights>2001 American Association of Orthodontists</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-3db3b2bb0d44c943205cd469874a4981a08e3dfc1e7db8776ab47f9b1ea78abe3</citedby><cites>FETCH-LOGICAL-c409t-3db3b2bb0d44c943205cd469874a4981a08e3dfc1e7db8776ab47f9b1ea78abe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1067/mod.2001.113654$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11343024$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rana, Mohammed Waheed-uz-Zaman</creatorcontrib><creatorcontrib>Pothisiri, Vighanda</creatorcontrib><creatorcontrib>Killiany, Dennis Michael</creatorcontrib><creatorcontrib>Xu, Xiao Ming</creatorcontrib><title>Detection of apoptosis during orthodontic tooth movement in rats</title><title>American journal of orthodontics and dentofacial orthopedics</title><addtitle>Am J Orthod Dentofacial Orthop</addtitle><description>Experimental evidence suggests that cellular changes and tissue turnover occurs in the periapical tissue during tooth movement. The possible role of apoptosis in tooth movement has never been investigated. The purpose of this study was to show whether apoptosis plays any role in orthodontic tooth movement. A sample of 21 adult male Sprague-Dawley rats was divided into 4 groups. Orthodontic appliances were attached to the rats for various times—to group I (n = 6) for 3 days, to group II (n = 6) for 1 week, and to group III (n = 6) for 2 weeks. Rats in group IV (n = 3) had appliances but no force and served as the control group. At the end of the observation period, the animals were killed and tissue blocks of involved teeth were processed and prepared for TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) reaction. TUNEL-positive cells from selected areas on each slide were counted and compared by means of an analysis of variance. Group I demonstrated a significantly higher (P <.05) number of positive cells. This suggests that the maximum apoptosis occurs approximately 3 days after the application of the appliance. (Am J Orthod Dentofacial Orthop 2001;119:516-21)</description><subject>Alveolar Bone Loss - etiology</subject><subject>Alveolar Bone Loss - pathology</subject><subject>Analysis of Variance</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Dental Pulp - pathology</subject><subject>Dental Stress Analysis</subject><subject>Dentistry</subject><subject>In Situ Nick-End Labeling</subject><subject>Male</subject><subject>Maxilla</subject><subject>Molar - pathology</subject><subject>Orthodontic Appliances - adverse effects</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Statistics, Nonparametric</subject><subject>Time Factors</subject><subject>Tooth Movement Techniques - adverse effects</subject><issn>0889-5406</issn><issn>1097-6752</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kDtPwzAURi0EoqUws6FMbGmvEyeON1B5SpVYYLb8uKFGTVxstxL_nlSpxMR0l3OP9B1CrinMKdR80Xk7LwDonNKyrtgJmVIQPK95VZySKTSNyCsG9YRcxPgFAIIVcE4mA81KKNiU3D1gQpOc7zPfZmrrt8lHFzO7C67_zHxIa299n5zJkvdpnXV-jx32KXN9FlSKl-SsVZuIV8c7Ix9Pj-_Ll3z19vy6vF_lhoFIeWl1qQutwTJmBCsLqIxltWg4U0w0VEGDpW0NRW51w3mtNOOt0BQVb5TGckZuR-82-O8dxiQ7Fw1uNqpHv4uSQ1PUdSUGcDGCJvgYA7ZyG1ynwo-kIA_R5BBNHqLJMdrwcXNU73SH9o8_VhoAMQI4DNw7DDIah71B68IQT1rv_pX_ApRue8Y</recordid><startdate>20010501</startdate><enddate>20010501</enddate><creator>Rana, Mohammed Waheed-uz-Zaman</creator><creator>Pothisiri, Vighanda</creator><creator>Killiany, Dennis Michael</creator><creator>Xu, Xiao Ming</creator><general>Mosby, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010501</creationdate><title>Detection of apoptosis during orthodontic tooth movement in rats</title><author>Rana, Mohammed Waheed-uz-Zaman ; Pothisiri, Vighanda ; Killiany, Dennis Michael ; Xu, Xiao Ming</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-3db3b2bb0d44c943205cd469874a4981a08e3dfc1e7db8776ab47f9b1ea78abe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Alveolar Bone Loss - etiology</topic><topic>Alveolar Bone Loss - pathology</topic><topic>Analysis of Variance</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Dental Pulp - pathology</topic><topic>Dental Stress Analysis</topic><topic>Dentistry</topic><topic>In Situ Nick-End Labeling</topic><topic>Male</topic><topic>Maxilla</topic><topic>Molar - pathology</topic><topic>Orthodontic Appliances - adverse effects</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Statistics, Nonparametric</topic><topic>Time Factors</topic><topic>Tooth Movement Techniques - adverse effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rana, Mohammed Waheed-uz-Zaman</creatorcontrib><creatorcontrib>Pothisiri, Vighanda</creatorcontrib><creatorcontrib>Killiany, Dennis Michael</creatorcontrib><creatorcontrib>Xu, Xiao Ming</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of orthodontics and dentofacial orthopedics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rana, Mohammed Waheed-uz-Zaman</au><au>Pothisiri, Vighanda</au><au>Killiany, Dennis Michael</au><au>Xu, Xiao Ming</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of apoptosis during orthodontic tooth movement in rats</atitle><jtitle>American journal of orthodontics and dentofacial orthopedics</jtitle><addtitle>Am J Orthod Dentofacial Orthop</addtitle><date>2001-05-01</date><risdate>2001</risdate><volume>119</volume><issue>5</issue><spage>516</spage><epage>521</epage><pages>516-521</pages><issn>0889-5406</issn><eissn>1097-6752</eissn><abstract>Experimental evidence suggests that cellular changes and tissue turnover occurs in the periapical tissue during tooth movement. The possible role of apoptosis in tooth movement has never been investigated. The purpose of this study was to show whether apoptosis plays any role in orthodontic tooth movement. A sample of 21 adult male Sprague-Dawley rats was divided into 4 groups. Orthodontic appliances were attached to the rats for various times—to group I (n = 6) for 3 days, to group II (n = 6) for 1 week, and to group III (n = 6) for 2 weeks. Rats in group IV (n = 3) had appliances but no force and served as the control group. At the end of the observation period, the animals were killed and tissue blocks of involved teeth were processed and prepared for TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) reaction. TUNEL-positive cells from selected areas on each slide were counted and compared by means of an analysis of variance. Group I demonstrated a significantly higher (P <.05) number of positive cells. This suggests that the maximum apoptosis occurs approximately 3 days after the application of the appliance. (Am J Orthod Dentofacial Orthop 2001;119:516-21)</abstract><cop>United States</cop><pub>Mosby, Inc</pub><pmid>11343024</pmid><doi>10.1067/mod.2001.113654</doi><tpages>6</tpages></addata></record> |
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| ispartof | American journal of orthodontics and dentofacial orthopedics, 2001-05, Vol.119 (5), p.516-521 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Alveolar Bone Loss - etiology Alveolar Bone Loss - pathology Analysis of Variance Animals Apoptosis Dental Pulp - pathology Dental Stress Analysis Dentistry In Situ Nick-End Labeling Male Maxilla Molar - pathology Orthodontic Appliances - adverse effects Rats Rats, Sprague-Dawley Statistics, Nonparametric Time Factors Tooth Movement Techniques - adverse effects |
| title | Detection of apoptosis during orthodontic tooth movement in rats |
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