Localization of Neuronal Growth-Associated, Microtubule-Destabilizing Factor SCG10 in Brain-Derived Raft Membrane Microdomains
Raft is a mobile membrane subdomain enriched in sphingolipid and cholesterol and also various signaling molecules. Previous observation suggested that brain-derived rafts contain tubulin but that rafts of non-neural origin do not. We hypothesized that SCG10, one of the neuronal growth-associated pro...
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| Veröffentlicht in: | Journal of biochemistry (Tokyo) 2001-05, Vol.129 (5), p.691-697 |
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| container_title | Journal of biochemistry (Tokyo) |
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| creator | Maekawa, Shohei Morii, Hiroshi Kumanogoh, Haruko Sano, Mayuko Naruse, Yoshihisa Sokawa, Yoshihiro Mori, Nozomu |
| description | Raft is a mobile membrane subdomain enriched in sphingolipid and cholesterol and also various signaling molecules. Previous observation suggested that brain-derived rafts contain tubulin but that rafts of non-neural origin do not. We hypothesized that SCG10, one of the neuronal growth-associated proteins (nGAPs), might be a neuron-specific molecule that anchors tubulin to neuronal rafts, and we explored biochemically its subcellular localization, interaction with tubulin, and effects on microtubule dynamics. In postnatal rat brain extracts, SCG10 was recovered mostly in membrane-associated fractions, and at least half was included in the raft fraction that was also enriched in GAP-43 and NAP-22. SCG1O-enriched brain rafts also contained tubulin, and chemical cross-linking experiments revealed that SCG10 was closely associated with tubulin. In addition, SCG10 was able to inhibit polymerization of tubulin. These results indicate that SCG10 is a component of neuronal rafts as are other nGAPs, and suggest that SCG10 may be involved in signaling events in membranes for cytoskeletal reorganization around neuronal rafts. |
| doi_str_mv | 10.1093/oxfordjournals.jbchem.a002908 |
| format | Article |
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Previous observation suggested that brain-derived rafts contain tubulin but that rafts of non-neural origin do not. We hypothesized that SCG10, one of the neuronal growth-associated proteins (nGAPs), might be a neuron-specific molecule that anchors tubulin to neuronal rafts, and we explored biochemically its subcellular localization, interaction with tubulin, and effects on microtubule dynamics. In postnatal rat brain extracts, SCG10 was recovered mostly in membrane-associated fractions, and at least half was included in the raft fraction that was also enriched in GAP-43 and NAP-22. SCG1O-enriched brain rafts also contained tubulin, and chemical cross-linking experiments revealed that SCG10 was closely associated with tubulin. In addition, SCG10 was able to inhibit polymerization of tubulin. 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Previous observation suggested that brain-derived rafts contain tubulin but that rafts of non-neural origin do not. We hypothesized that SCG10, one of the neuronal growth-associated proteins (nGAPs), might be a neuron-specific molecule that anchors tubulin to neuronal rafts, and we explored biochemically its subcellular localization, interaction with tubulin, and effects on microtubule dynamics. In postnatal rat brain extracts, SCG10 was recovered mostly in membrane-associated fractions, and at least half was included in the raft fraction that was also enriched in GAP-43 and NAP-22. SCG1O-enriched brain rafts also contained tubulin, and chemical cross-linking experiments revealed that SCG10 was closely associated with tubulin. In addition, SCG10 was able to inhibit polymerization of tubulin. These results indicate that SCG10 is a component of neuronal rafts as are other nGAPs, and suggest that SCG10 may be involved in signaling events in membranes for cytoskeletal reorganization around neuronal rafts.</description><subject>Animals</subject><subject>Brain - metabolism</subject><subject>Carrier Proteins</subject><subject>caveolae</subject><subject>Cytoskeleton - chemistry</subject><subject>Cytoskeleton - metabolism</subject><subject>Membrane Microdomains - chemistry</subject><subject>Membrane Microdomains - metabolism</subject><subject>Membrane Proteins</subject><subject>Microtubules - chemistry</subject><subject>Microtubules - drug effects</subject><subject>Microtubules - metabolism</subject><subject>Nerve Growth Factors - chemistry</subject><subject>Nerve Growth Factors - metabolism</subject><subject>Nerve Growth Factors - pharmacology</subject><subject>neuron</subject><subject>Neurons - chemistry</subject><subject>Neurons - metabolism</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Signal Transduction - physiology</subject><subject>signaling</subject><subject>stathmin</subject><subject>Subcellular Fractions - chemistry</subject><subject>tubulin</subject><subject>Tubulin - chemistry</subject><issn>0021-924X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkM1PFDEYxnvACK7-C6QXODlrO-189MABV1jQRRNdkg2Xpp_QdWaKbQfRg387JbPReHr7ps_zvE9-ABxhNMeIkXf-0fqgt34Mg-jifCvVnennAqGSoXYPHOQHLlhJN_vgVYzb57Uk5CXYx5iUbcXQAfiz8kp07rdIzg_QW_jZjMHnOLgM_me6K05j9MqJZPRbeOVU8GmUY2eKDyYmIV22uuEWnguVfIDfFkuMoBvg-yDckDXBPRgNvwqb4JXpZRCDmVK077MivgYvbK5u3uzmDFyfn60XF8Xqy_JycboqFK3qVNhaYm0M0y2lla0VZbSpmGglKgkl1LaibFpmlcaEYqkkwcIqo1qs68oiZcgMHE-598H_GHN13ruoTNflQn6MvEFtWdUZzgycTMLcMcZgLL8PrhfhF8eIP0Pn_0PnE3S-g579h7tDo-yN_ufeEc-CYhK4mMzj338RvvO6IU3FLzY3_CPbrD-t88UVeQJA8pmL</recordid><startdate>20010501</startdate><enddate>20010501</enddate><creator>Maekawa, Shohei</creator><creator>Morii, Hiroshi</creator><creator>Kumanogoh, Haruko</creator><creator>Sano, Mayuko</creator><creator>Naruse, Yoshihisa</creator><creator>Sokawa, Yoshihiro</creator><creator>Mori, Nozomu</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010501</creationdate><title>Localization of Neuronal Growth-Associated, Microtubule-Destabilizing Factor SCG10 in Brain-Derived Raft Membrane Microdomains</title><author>Maekawa, Shohei ; Morii, Hiroshi ; Kumanogoh, Haruko ; Sano, Mayuko ; Naruse, Yoshihisa ; Sokawa, Yoshihiro ; Mori, Nozomu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-f6b1dee9d8445f6c494759a8b023434f8a2789fcd1341bcb31afcec81d65f0ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Brain - metabolism</topic><topic>Carrier Proteins</topic><topic>caveolae</topic><topic>Cytoskeleton - chemistry</topic><topic>Cytoskeleton - metabolism</topic><topic>Membrane Microdomains - chemistry</topic><topic>Membrane Microdomains - metabolism</topic><topic>Membrane Proteins</topic><topic>Microtubules - chemistry</topic><topic>Microtubules - drug effects</topic><topic>Microtubules - metabolism</topic><topic>Nerve Growth Factors - chemistry</topic><topic>Nerve Growth Factors - metabolism</topic><topic>Nerve Growth Factors - pharmacology</topic><topic>neuron</topic><topic>Neurons - chemistry</topic><topic>Neurons - metabolism</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Signal Transduction - physiology</topic><topic>signaling</topic><topic>stathmin</topic><topic>Subcellular Fractions - chemistry</topic><topic>tubulin</topic><topic>Tubulin - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maekawa, Shohei</creatorcontrib><creatorcontrib>Morii, Hiroshi</creatorcontrib><creatorcontrib>Kumanogoh, Haruko</creatorcontrib><creatorcontrib>Sano, Mayuko</creatorcontrib><creatorcontrib>Naruse, Yoshihisa</creatorcontrib><creatorcontrib>Sokawa, Yoshihiro</creatorcontrib><creatorcontrib>Mori, Nozomu</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biochemistry (Tokyo)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maekawa, Shohei</au><au>Morii, Hiroshi</au><au>Kumanogoh, Haruko</au><au>Sano, Mayuko</au><au>Naruse, Yoshihisa</au><au>Sokawa, Yoshihiro</au><au>Mori, Nozomu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Localization of Neuronal Growth-Associated, Microtubule-Destabilizing Factor SCG10 in Brain-Derived Raft Membrane Microdomains</atitle><jtitle>Journal of biochemistry (Tokyo)</jtitle><addtitle>J Biochem</addtitle><date>2001-05-01</date><risdate>2001</risdate><volume>129</volume><issue>5</issue><spage>691</spage><epage>697</epage><pages>691-697</pages><issn>0021-924X</issn><abstract>Raft is a mobile membrane subdomain enriched in sphingolipid and cholesterol and also various signaling molecules. Previous observation suggested that brain-derived rafts contain tubulin but that rafts of non-neural origin do not. We hypothesized that SCG10, one of the neuronal growth-associated proteins (nGAPs), might be a neuron-specific molecule that anchors tubulin to neuronal rafts, and we explored biochemically its subcellular localization, interaction with tubulin, and effects on microtubule dynamics. In postnatal rat brain extracts, SCG10 was recovered mostly in membrane-associated fractions, and at least half was included in the raft fraction that was also enriched in GAP-43 and NAP-22. SCG1O-enriched brain rafts also contained tubulin, and chemical cross-linking experiments revealed that SCG10 was closely associated with tubulin. In addition, SCG10 was able to inhibit polymerization of tubulin. 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| subjects | Animals Brain - metabolism Carrier Proteins caveolae Cytoskeleton - chemistry Cytoskeleton - metabolism Membrane Microdomains - chemistry Membrane Microdomains - metabolism Membrane Proteins Microtubules - chemistry Microtubules - drug effects Microtubules - metabolism Nerve Growth Factors - chemistry Nerve Growth Factors - metabolism Nerve Growth Factors - pharmacology neuron Neurons - chemistry Neurons - metabolism Rats Rats, Wistar Signal Transduction - physiology signaling stathmin Subcellular Fractions - chemistry tubulin Tubulin - chemistry |
| title | Localization of Neuronal Growth-Associated, Microtubule-Destabilizing Factor SCG10 in Brain-Derived Raft Membrane Microdomains |
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