Ca(2+)-dependent gene expression mediated by MEF2 transcription factors
Ca(2+) induction of a subset of cellular and viral immediate-early activation genes in lymphocytes has been previously mapped to response elements recognized by the MEF2 family of transcription factors. Here, we demonstrate that Ca(2+) activation of MEF2 response elements in T lymphocytes is mediate...
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| Veröffentlicht in: | The Journal of biological chemistry 2000-01, Vol.275 (1), p.197-209 |
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| container_title | The Journal of biological chemistry |
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| creator | Blaeser, F Ho, N Prywes, R Chatila, T A |
| description | Ca(2+) induction of a subset of cellular and viral immediate-early activation genes in lymphocytes has been previously mapped to response elements recognized by the MEF2 family of transcription factors. Here, we demonstrate that Ca(2+) activation of MEF2 response elements in T lymphocytes is mediated in synergy by two Ca(2+)/calmodulin-dependent enzymes, the phosphatase calcineurin, and the kinase type IV/Gr (CaMKIV/Gr), which promote transcription by the MEF2 family members MEF2A and MEF2D. Calcineurin up-regulates the activity of both factors by an NFAT-dependent mechanism, while CaMKIV/Gr selectively and independently activates MEF2D. These results identify MEF2 proteins as effectors of a pathway of gene induction in T lymphocytes which integrates diverse Ca(2+) activation signals and may be broadly operative in several tissues. |
| doi_str_mv | 10.1074/jbc.275.1.197 |
| format | Article |
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These results identify MEF2 proteins as effectors of a pathway of gene induction in T lymphocytes which integrates diverse Ca(2+) activation signals and may be broadly operative in several tissues.</description><identifier>ISSN: 0021-9258</identifier><identifier>DOI: 10.1074/jbc.275.1.197</identifier><identifier>PMID: 10617605</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Base Sequence ; Calcineurin - metabolism ; Calcium Signaling ; Calcium-Calmodulin-Dependent Protein Kinase Type 4 ; Calcium-Calmodulin-Dependent Protein Kinases - metabolism ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; Gene Expression Regulation ; Humans ; MADS Domain Proteins ; MEF2 Transcription Factors ; Mice ; Molecular Sequence Data ; Myogenic Regulatory Factors ; NFATC Transcription Factors ; Nuclear Proteins ; Nuclear Receptor Subfamily 4, Group A, Member 1 ; Protein Binding ; Receptors, Cytoplasmic and Nuclear ; Receptors, Steroid ; Recombinant Proteins - metabolism ; Response Elements ; T-Lymphocytes - metabolism ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Transcriptional Activation</subject><ispartof>The Journal of biological chemistry, 2000-01, Vol.275 (1), p.197-209</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10617605$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Blaeser, F</creatorcontrib><creatorcontrib>Ho, N</creatorcontrib><creatorcontrib>Prywes, R</creatorcontrib><creatorcontrib>Chatila, T A</creatorcontrib><title>Ca(2+)-dependent gene expression mediated by MEF2 transcription factors</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Ca(2+) induction of a subset of cellular and viral immediate-early activation genes in lymphocytes has been previously mapped to response elements recognized by the MEF2 family of transcription factors. 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These results identify MEF2 proteins as effectors of a pathway of gene induction in T lymphocytes which integrates diverse Ca(2+) activation signals and may be broadly operative in several tissues.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Calcineurin - metabolism</subject><subject>Calcium Signaling</subject><subject>Calcium-Calmodulin-Dependent Protein Kinase Type 4</subject><subject>Calcium-Calmodulin-Dependent Protein Kinases - metabolism</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Gene Expression Regulation</subject><subject>Humans</subject><subject>MADS Domain Proteins</subject><subject>MEF2 Transcription Factors</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Myogenic Regulatory Factors</subject><subject>NFATC Transcription Factors</subject><subject>Nuclear Proteins</subject><subject>Nuclear Receptor Subfamily 4, Group A, Member 1</subject><subject>Protein Binding</subject><subject>Receptors, Cytoplasmic and Nuclear</subject><subject>Receptors, Steroid</subject><subject>Recombinant Proteins - metabolism</subject><subject>Response Elements</subject><subject>T-Lymphocytes - metabolism</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Transcriptional Activation</subject><issn>0021-9258</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1jzFPwzAUhD2AaCmMrCgTAqEEPzuOkxFVbUEqYoE5erZfUKo2CbYj0X_fIsotN9ynkz7GboBnwHX-tDE2E1plkEGlz9iUcwFpJVQ5YZchbPgxeQUXbAK8AF1wNWWrOd6Lx4fU0UCdoy4mX9RRQj-DpxDavkt25FqM5BKzT94WS5FEj12wvh3i79ygjb0PV-y8wW2g61PP2Ody8TF_Sdfvq9f58zodQIiYukoBWN5IbkprrauwRKeUaUCqxhljsMmFzAVHV2gjtHalQEBboCmMkihn7O7vd_D990gh1rs2WNpusaN-DLXmpVC51Efw9gSO5qhQD77dod_X_-ryAFQGWQI</recordid><startdate>20000107</startdate><enddate>20000107</enddate><creator>Blaeser, F</creator><creator>Ho, N</creator><creator>Prywes, R</creator><creator>Chatila, T A</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20000107</creationdate><title>Ca(2+)-dependent gene expression mediated by MEF2 transcription factors</title><author>Blaeser, F ; 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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Base Sequence Calcineurin - metabolism Calcium Signaling Calcium-Calmodulin-Dependent Protein Kinase Type 4 Calcium-Calmodulin-Dependent Protein Kinases - metabolism DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Gene Expression Regulation Humans MADS Domain Proteins MEF2 Transcription Factors Mice Molecular Sequence Data Myogenic Regulatory Factors NFATC Transcription Factors Nuclear Proteins Nuclear Receptor Subfamily 4, Group A, Member 1 Protein Binding Receptors, Cytoplasmic and Nuclear Receptors, Steroid Recombinant Proteins - metabolism Response Elements T-Lymphocytes - metabolism Transcription Factors - genetics Transcription Factors - metabolism Transcriptional Activation |
| title | Ca(2+)-dependent gene expression mediated by MEF2 transcription factors |
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