Performance comparison of Protein A affinity-chromatography sorbents for purifying recombinant monoclonal antibodies

We describe the performance characteristics of five Protein A affinity‐chromatography sorbents (Sepharose Fast Flow, Poros 50, Poros LP, Prosep and Streamline) for purifying a recombinant humanized monoclonal antibody from clarified Chinese hamster ovary cell culture fluid. We measured the dynamic c...

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Veröffentlicht in:	Biotechnology and applied biochemistry 1999-10, Vol.30 (2), p.121-128
Hauptverfasser:	Fahrner, Robert L., Whitney, Duncan H., Vanderlaan, Martin, Blank, Gregory S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Monoclonal - isolation & purification Antibodies, Monoclonal - metabolism Biological and medical sciences Biotechnology CHO Cells Chromatography, Affinity - instrumentation Chromatography, Affinity - methods Chromatography, Agarose - instrumentation Chromatography, Agarose - methods Cricetinae Electrophoresis, Polyacrylamide Gel - methods Fundamental and applied biological sciences. Psychology Health. Pharmaceutical industry Industrial applications and implications. Economical aspects Monoclonal antibodies Production of active biomolecules protein A Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Staphylococcal Protein A - metabolism
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