Ultrastructural Characterisation and Molecular Taxonomic Identification of Nosema granulosis n. sp., a Transovarially Transmitted Feminising (TTF) Microsporidium
ABSTRACT A novel microsporidian parasite is described, which infects the crustacean host Gammarus duebeni. The parasite was transovarially transmitted and feminised host offspring. The life cycle was monomorphic with three stages. Meronts were found in host embryos, juveniles, and in the gonadal tis...
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| Veröffentlicht in: | The Journal of eukaryotic microbiology 1999-09, Vol.46 (5), p.492-499 |
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| container_title | The Journal of eukaryotic microbiology |
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| creator | TERRY, REBECCA S. SMITH, JUDITH E. BOUCHON, DIDIER RIGAUD, THIERRY DUNCANSON, PHIL SHARPE, ROSE G. DUNN, ALISON M. |
| description | ABSTRACT
A novel microsporidian parasite is described, which infects the crustacean host Gammarus duebeni. The parasite was transovarially transmitted and feminised host offspring. The life cycle was monomorphic with three stages. Meronts were found in host embryos, juveniles, and in the gonadal tissue of adults. Sporoblasts and spores were restricted to the gonad. Sporogony was disporoblastic giving rise to paired sporoblasts, which then differentiated to form spores. Spores were not found in regular groupings and there was no interfacial envelope. Spores were approximately 3.78 × 1.22 μm and had a thin exospore wall, a short polar filament, and an unusual granular polaroplast. All life cycle stages were diplokaryotic. A region from the parasite small subunit ribosomal RNA gene was amplified and sequenced. Phylogenetic analysis based on these data places the parasite within the genus Nosema. We have named the species Nosema granulosis based on the structure of the polaroplast. |
| doi_str_mv | 10.1111/j.1550-7408.1999.tb06066.x |
| format | Article |
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A novel microsporidian parasite is described, which infects the crustacean host Gammarus duebeni. The parasite was transovarially transmitted and feminised host offspring. The life cycle was monomorphic with three stages. Meronts were found in host embryos, juveniles, and in the gonadal tissue of adults. Sporoblasts and spores were restricted to the gonad. Sporogony was disporoblastic giving rise to paired sporoblasts, which then differentiated to form spores. Spores were not found in regular groupings and there was no interfacial envelope. Spores were approximately 3.78 × 1.22 μm and had a thin exospore wall, a short polar filament, and an unusual granular polaroplast. All life cycle stages were diplokaryotic. A region from the parasite small subunit ribosomal RNA gene was amplified and sequenced. Phylogenetic analysis based on these data places the parasite within the genus Nosema. We have named the species Nosema granulosis based on the structure of the polaroplast.</description><identifier>ISSN: 1066-5234</identifier><identifier>EISSN: 1550-7408</identifier><identifier>DOI: 10.1111/j.1550-7408.1999.tb06066.x</identifier><identifier>PMID: 10519217</identifier><identifier>CODEN: JEMIED</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Biological and medical sciences ; Comparative sequence analysis ; Crustacea - parasitology ; DNA, Protozoan - analysis ; Female ; Fundamental and applied biological sciences. Psychology ; Gammarus duebeni ; Life Cycle Stages ; Microscopy, Electron ; Microsporidia ; Molecular Sequence Data ; Nosema - classification ; Nosema - genetics ; Nosema - growth & development ; Nosema - ultrastructure ; Nosema granulosis ; Ovary - parasitology ; Phylogeny ; Polymerase Chain Reaction ; Protozoa ; RNA, Ribosomal - genetics ; Sequence Analysis, DNA ; Spores - growth & development ; Spores - ultrastructure ; SSU rRNA ; Systematics. Geographical distribution. Morphology. Cytology ; transovarial transmission</subject><ispartof>The Journal of eukaryotic microbiology, 1999-09, Vol.46 (5), p.492-499</ispartof><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4682-d1ff64f4801166626f9d136c7027767309ae463e002c883480b9f09d27929dcd3</citedby><cites>FETCH-LOGICAL-c4682-d1ff64f4801166626f9d136c7027767309ae463e002c883480b9f09d27929dcd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1550-7408.1999.tb06066.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1550-7408.1999.tb06066.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>309,310,314,780,784,789,790,1417,23930,23931,25140,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1977827$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10519217$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>TERRY, REBECCA S.</creatorcontrib><creatorcontrib>SMITH, JUDITH E.</creatorcontrib><creatorcontrib>BOUCHON, DIDIER</creatorcontrib><creatorcontrib>RIGAUD, THIERRY</creatorcontrib><creatorcontrib>DUNCANSON, PHIL</creatorcontrib><creatorcontrib>SHARPE, ROSE G.</creatorcontrib><creatorcontrib>DUNN, ALISON M.</creatorcontrib><title>Ultrastructural Characterisation and Molecular Taxonomic Identification of Nosema granulosis n. sp., a Transovarially Transmitted Feminising (TTF) Microsporidium</title><title>The Journal of eukaryotic microbiology</title><addtitle>J Eukaryot Microbiol</addtitle><description>ABSTRACT
A novel microsporidian parasite is described, which infects the crustacean host Gammarus duebeni. The parasite was transovarially transmitted and feminised host offspring. The life cycle was monomorphic with three stages. Meronts were found in host embryos, juveniles, and in the gonadal tissue of adults. Sporoblasts and spores were restricted to the gonad. Sporogony was disporoblastic giving rise to paired sporoblasts, which then differentiated to form spores. Spores were not found in regular groupings and there was no interfacial envelope. Spores were approximately 3.78 × 1.22 μm and had a thin exospore wall, a short polar filament, and an unusual granular polaroplast. All life cycle stages were diplokaryotic. A region from the parasite small subunit ribosomal RNA gene was amplified and sequenced. Phylogenetic analysis based on these data places the parasite within the genus Nosema. We have named the species Nosema granulosis based on the structure of the polaroplast.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Comparative sequence analysis</subject><subject>Crustacea - parasitology</subject><subject>DNA, Protozoan - analysis</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gammarus duebeni</subject><subject>Life Cycle Stages</subject><subject>Microscopy, Electron</subject><subject>Microsporidia</subject><subject>Molecular Sequence Data</subject><subject>Nosema - classification</subject><subject>Nosema - genetics</subject><subject>Nosema - growth & development</subject><subject>Nosema - ultrastructure</subject><subject>Nosema granulosis</subject><subject>Ovary - parasitology</subject><subject>Phylogeny</subject><subject>Polymerase Chain Reaction</subject><subject>Protozoa</subject><subject>RNA, Ribosomal - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Spores - growth & development</subject><subject>Spores - ultrastructure</subject><subject>SSU rRNA</subject><subject>Systematics. Geographical distribution. Morphology. Cytology</subject><subject>transovarial transmission</subject><issn>1066-5234</issn><issn>1550-7408</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkd-O1CAUxhujcf_oKxhijFkTW4G2ULwwMZOddXV3je5MTLwhDNCVWVpGoDrzOL6pNJ2s3hm5gXP4ne_A-bLsKYIFSuvVukB1DXNawaZAjLEiriCBhBTbe9nh3dX9dE7JvMZldZAdhbCGEBGM0MPsAMEaMYzoYfZraaMXIfpBxsELC2bfhBcyam-CiMb1QPQKXDqr5WCFBwuxdb3rjATnSvfRtEZOmGvBlQu6E-DGi36wLpgA-gKETfESCLBIyeB-CG-Etbsp7EyMWoG57kxvgulvwMliMX8BLo30LmycN8oM3aPsQSts0I_3-3G2nJ8uZu_yi49n57O3F7msSINzhdqWVG3VQIQIIZi0TKGSSAoxpYSWkAldkVJDiGXTlIlbsRYyhSnDTElVHmfPJ92Nd98HHSLvTJDaWtFrNwROYYNTp-qfIKI1LGndJPD1BI7fCV63fONNJ_yOI8hHJ_maj3bx0S4-Osn3TvJtKn6y7zKsOq3-Kp2sS8CzPSCCFLZNE5Um_OEYpQ0esTcT9tNYvfuPF_D3p8uK4SSQTwImRL29ExD-lqex0pp_uTrjn6_x10_XEPMP5W_6xcwz</recordid><startdate>199909</startdate><enddate>199909</enddate><creator>TERRY, REBECCA S.</creator><creator>SMITH, JUDITH E.</creator><creator>BOUCHON, DIDIER</creator><creator>RIGAUD, THIERRY</creator><creator>DUNCANSON, PHIL</creator><creator>SHARPE, ROSE G.</creator><creator>DUNN, ALISON M.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>199909</creationdate><title>Ultrastructural Characterisation and Molecular Taxonomic Identification of Nosema granulosis n. sp., a Transovarially Transmitted Feminising (TTF) Microsporidium</title><author>TERRY, REBECCA S. ; SMITH, JUDITH E. ; BOUCHON, DIDIER ; RIGAUD, THIERRY ; DUNCANSON, PHIL ; SHARPE, ROSE G. ; DUNN, ALISON M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4682-d1ff64f4801166626f9d136c7027767309ae463e002c883480b9f09d27929dcd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Comparative sequence analysis</topic><topic>Crustacea - parasitology</topic><topic>DNA, Protozoan - analysis</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gammarus duebeni</topic><topic>Life Cycle Stages</topic><topic>Microscopy, Electron</topic><topic>Microsporidia</topic><topic>Molecular Sequence Data</topic><topic>Nosema - classification</topic><topic>Nosema - genetics</topic><topic>Nosema - growth & development</topic><topic>Nosema - ultrastructure</topic><topic>Nosema granulosis</topic><topic>Ovary - parasitology</topic><topic>Phylogeny</topic><topic>Polymerase Chain Reaction</topic><topic>Protozoa</topic><topic>RNA, Ribosomal - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Spores - growth & development</topic><topic>Spores - ultrastructure</topic><topic>SSU rRNA</topic><topic>Systematics. Geographical distribution. Morphology. Cytology</topic><topic>transovarial transmission</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>TERRY, REBECCA S.</creatorcontrib><creatorcontrib>SMITH, JUDITH E.</creatorcontrib><creatorcontrib>BOUCHON, DIDIER</creatorcontrib><creatorcontrib>RIGAUD, THIERRY</creatorcontrib><creatorcontrib>DUNCANSON, PHIL</creatorcontrib><creatorcontrib>SHARPE, ROSE G.</creatorcontrib><creatorcontrib>DUNN, ALISON M.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of eukaryotic microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>TERRY, REBECCA S.</au><au>SMITH, JUDITH E.</au><au>BOUCHON, DIDIER</au><au>RIGAUD, THIERRY</au><au>DUNCANSON, PHIL</au><au>SHARPE, ROSE G.</au><au>DUNN, ALISON M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultrastructural Characterisation and Molecular Taxonomic Identification of Nosema granulosis n. sp., a Transovarially Transmitted Feminising (TTF) Microsporidium</atitle><jtitle>The Journal of eukaryotic microbiology</jtitle><addtitle>J Eukaryot Microbiol</addtitle><date>1999-09</date><risdate>1999</risdate><volume>46</volume><issue>5</issue><spage>492</spage><epage>499</epage><pages>492-499</pages><issn>1066-5234</issn><eissn>1550-7408</eissn><coden>JEMIED</coden><abstract>ABSTRACT
A novel microsporidian parasite is described, which infects the crustacean host Gammarus duebeni. The parasite was transovarially transmitted and feminised host offspring. The life cycle was monomorphic with three stages. Meronts were found in host embryos, juveniles, and in the gonadal tissue of adults. Sporoblasts and spores were restricted to the gonad. Sporogony was disporoblastic giving rise to paired sporoblasts, which then differentiated to form spores. Spores were not found in regular groupings and there was no interfacial envelope. Spores were approximately 3.78 × 1.22 μm and had a thin exospore wall, a short polar filament, and an unusual granular polaroplast. All life cycle stages were diplokaryotic. A region from the parasite small subunit ribosomal RNA gene was amplified and sequenced. Phylogenetic analysis based on these data places the parasite within the genus Nosema. We have named the species Nosema granulosis based on the structure of the polaroplast.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>10519217</pmid><doi>10.1111/j.1550-7408.1999.tb06066.x</doi><tpages>8</tpages></addata></record> |
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| identifier | ISSN: 1066-5234 |
| ispartof | The Journal of eukaryotic microbiology, 1999-09, Vol.46 (5), p.492-499 |
| issn | 1066-5234 1550-7408 |
| language | eng |
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| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Animals Biological and medical sciences Comparative sequence analysis Crustacea - parasitology DNA, Protozoan - analysis Female Fundamental and applied biological sciences. Psychology Gammarus duebeni Life Cycle Stages Microscopy, Electron Microsporidia Molecular Sequence Data Nosema - classification Nosema - genetics Nosema - growth & development Nosema - ultrastructure Nosema granulosis Ovary - parasitology Phylogeny Polymerase Chain Reaction Protozoa RNA, Ribosomal - genetics Sequence Analysis, DNA Spores - growth & development Spores - ultrastructure SSU rRNA Systematics. Geographical distribution. Morphology. Cytology transovarial transmission |
| title | Ultrastructural Characterisation and Molecular Taxonomic Identification of Nosema granulosis n. sp., a Transovarially Transmitted Feminising (TTF) Microsporidium |
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