Diversity of core promoter mutations in immune clearance phase of chronic HBV infection
Transcription of HBV (hepatitis B virus) pre-core and pre-genomic mRNAs is controlled by core promoter. Therefore, mutations in the core promoter region might change the activity of liver diseases through an altered transcriptional level of the mRNA. The present study was carried out to determine th...
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| Veröffentlicht in: | European journal of gastroenterology & hepatology 1999-08, Vol.11 (8), p.821-826 |
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| container_title | European journal of gastroenterology & hepatology |
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| creator | Kim, Yun Soo Kim, Sung II Hwang, Seong Gyu Kim, Jin Oh Cho, Joo Young Lee, Joon Seong Lee, Moon Sung Hwang, Seung Duk Shim, Chan Sup |
| description | Transcription of HBV (hepatitis B virus) pre-core and pre-genomic mRNAs is controlled by core promoter. Therefore, mutations in the core promoter region might change the activity of liver diseases through an altered transcriptional level of the mRNA. The present study was carried out to determine the diversity of HBV core promoter sequences in chronic HBV carriers.
DNA sequences in the core promoter region were determined after cloning the PCR product. Two groups of chronic HBV carriers with HBeAg, including five cases of asymptomatic carriers (ASCs, 21 clones) and eight with chronic hepatitis (CH, 50 clones) were studied.
Mutations in the core promoter were found in three out of the ASCs (11 clones), and in all eight cases in the CH group (48 clones). While mutations at nucleotide 1762 (A-->T) and 1764(G-->A) were not found in ASC, mutations at the same positions were found in all the cases of CH group (40 clones) (P=0.003). Diverse patterns of mutations in the core promoter were observed in each patient in the CH group.
Further studies are needed to determine whether the diversity of HBV core promoter mutations has clinical significance such as the seroconversion of HBeAg to anti-HBe. |
| doi_str_mv | 10.1097/00042737-199908000-00002 |
| format | Article |
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DNA sequences in the core promoter region were determined after cloning the PCR product. Two groups of chronic HBV carriers with HBeAg, including five cases of asymptomatic carriers (ASCs, 21 clones) and eight with chronic hepatitis (CH, 50 clones) were studied.
Mutations in the core promoter were found in three out of the ASCs (11 clones), and in all eight cases in the CH group (48 clones). While mutations at nucleotide 1762 (A-->T) and 1764(G-->A) were not found in ASC, mutations at the same positions were found in all the cases of CH group (40 clones) (P=0.003). Diverse patterns of mutations in the core promoter were observed in each patient in the CH group.
Further studies are needed to determine whether the diversity of HBV core promoter mutations has clinical significance such as the seroconversion of HBeAg to anti-HBe.</description><identifier>ISSN: 0954-691X</identifier><identifier>EISSN: 1473-5687</identifier><identifier>DOI: 10.1097/00042737-199908000-00002</identifier><identifier>PMID: 10514111</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott Williams & Wilkins, Inc</publisher><subject>Adult ; Base Sequence ; Biological and medical sciences ; Carrier State - immunology ; Carrier State - virology ; Child ; DNA Mutational Analysis ; DNA, Viral - genetics ; Female ; Hepatitis B Antibodies - blood ; Hepatitis B e Antigens - blood ; Hepatitis B virus - genetics ; Hepatitis B virus - isolation & purification ; Hepatitis B, Chronic - immunology ; Hepatitis B, Chronic - virology ; Human viral diseases ; Humans ; Infectious diseases ; Male ; Medical sciences ; Middle Aged ; Molecular Sequence Data ; Mutation ; Polymerase Chain Reaction ; Promoter Regions, Genetic - genetics ; Tropical medicine ; Viral Core Proteins - genetics ; Viral diseases ; Viral hepatitis</subject><ispartof>European journal of gastroenterology & hepatology, 1999-08, Vol.11 (8), p.821-826</ispartof><rights>1999 Lippincott Williams & Wilkins, Inc.</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3852-8dde71b26c0b384357804c72934186141230e2eba4203ff14cf843936a4ae9523</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1922088$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10514111$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Yun Soo</creatorcontrib><creatorcontrib>Kim, Sung II</creatorcontrib><creatorcontrib>Hwang, Seong Gyu</creatorcontrib><creatorcontrib>Kim, Jin Oh</creatorcontrib><creatorcontrib>Cho, Joo Young</creatorcontrib><creatorcontrib>Lee, Joon Seong</creatorcontrib><creatorcontrib>Lee, Moon Sung</creatorcontrib><creatorcontrib>Hwang, Seung Duk</creatorcontrib><creatorcontrib>Shim, Chan Sup</creatorcontrib><title>Diversity of core promoter mutations in immune clearance phase of chronic HBV infection</title><title>European journal of gastroenterology & hepatology</title><addtitle>Eur J Gastroenterol Hepatol</addtitle><description>Transcription of HBV (hepatitis B virus) pre-core and pre-genomic mRNAs is controlled by core promoter. Therefore, mutations in the core promoter region might change the activity of liver diseases through an altered transcriptional level of the mRNA. The present study was carried out to determine the diversity of HBV core promoter sequences in chronic HBV carriers.
DNA sequences in the core promoter region were determined after cloning the PCR product. Two groups of chronic HBV carriers with HBeAg, including five cases of asymptomatic carriers (ASCs, 21 clones) and eight with chronic hepatitis (CH, 50 clones) were studied.
Mutations in the core promoter were found in three out of the ASCs (11 clones), and in all eight cases in the CH group (48 clones). While mutations at nucleotide 1762 (A-->T) and 1764(G-->A) were not found in ASC, mutations at the same positions were found in all the cases of CH group (40 clones) (P=0.003). Diverse patterns of mutations in the core promoter were observed in each patient in the CH group.
Further studies are needed to determine whether the diversity of HBV core promoter mutations has clinical significance such as the seroconversion of HBeAg to anti-HBe.</description><subject>Adult</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Carrier State - immunology</subject><subject>Carrier State - virology</subject><subject>Child</subject><subject>DNA Mutational Analysis</subject><subject>DNA, Viral - genetics</subject><subject>Female</subject><subject>Hepatitis B Antibodies - blood</subject><subject>Hepatitis B e Antigens - blood</subject><subject>Hepatitis B virus - genetics</subject><subject>Hepatitis B virus - isolation & purification</subject><subject>Hepatitis B, Chronic - immunology</subject><subject>Hepatitis B, Chronic - virology</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Polymerase Chain Reaction</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Tropical medicine</subject><subject>Viral Core Proteins - genetics</subject><subject>Viral diseases</subject><subject>Viral hepatitis</subject><issn>0954-691X</issn><issn>1473-5687</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kUtP3DAURq2qCIbHX6i8QN0Frh-J7SXlLSGxgZad5fHcaNwmMbUTEP8eDzMtbFhcWVc6n_3pmBDK4IiBUccAILkSqmLGGNBlrcoA_0JmTCpR1Y1WX8kMTC2rxrCHHbKb828ApgRT22SHQc0kY2xGfp2FJ0w5jC80ttTHhPQxxT6OmGg_jW4Mccg0DDT0_TQg9R265AZfsKXL-BZapjgET69-_Cxgi36V2SdbresyHmzOPXJ_cX53elXd3F5en57cVF7omld6sUDF5rzxMBdailppkF5xIyTTTenIBSDHuZMcRNsy6dtCGdE46dDUXOyR7-t7S-u_E-bR9iF77Do3YJyyVaC5hKYpoF6DPsWcE7b2MYXepRfLwK6k2n9S7X-p9k1qiX7bvDHNe1x8CK4tFuBwA7jsXdeuBIX8zhnOQeuCyTX2HLviN__ppmdMdomuG5f2sz8VrychjVA</recordid><startdate>199908</startdate><enddate>199908</enddate><creator>Kim, Yun Soo</creator><creator>Kim, Sung II</creator><creator>Hwang, Seong Gyu</creator><creator>Kim, Jin Oh</creator><creator>Cho, Joo Young</creator><creator>Lee, Joon Seong</creator><creator>Lee, Moon Sung</creator><creator>Hwang, Seung Duk</creator><creator>Shim, Chan Sup</creator><general>Lippincott Williams & Wilkins, Inc</general><general>Lippincott Williams & Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199908</creationdate><title>Diversity of core promoter mutations in immune clearance phase of chronic HBV infection</title><author>Kim, Yun Soo ; Kim, Sung II ; Hwang, Seong Gyu ; Kim, Jin Oh ; Cho, Joo Young ; Lee, Joon Seong ; Lee, Moon Sung ; Hwang, Seung Duk ; Shim, Chan Sup</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3852-8dde71b26c0b384357804c72934186141230e2eba4203ff14cf843936a4ae9523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Adult</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Carrier State - immunology</topic><topic>Carrier State - virology</topic><topic>Child</topic><topic>DNA Mutational Analysis</topic><topic>DNA, Viral - genetics</topic><topic>Female</topic><topic>Hepatitis B Antibodies - blood</topic><topic>Hepatitis B e Antigens - blood</topic><topic>Hepatitis B virus - genetics</topic><topic>Hepatitis B virus - isolation & purification</topic><topic>Hepatitis B, Chronic - immunology</topic><topic>Hepatitis B, Chronic - virology</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Polymerase Chain Reaction</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Tropical medicine</topic><topic>Viral Core Proteins - genetics</topic><topic>Viral diseases</topic><topic>Viral hepatitis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Yun Soo</creatorcontrib><creatorcontrib>Kim, Sung II</creatorcontrib><creatorcontrib>Hwang, Seong Gyu</creatorcontrib><creatorcontrib>Kim, Jin Oh</creatorcontrib><creatorcontrib>Cho, Joo Young</creatorcontrib><creatorcontrib>Lee, Joon Seong</creatorcontrib><creatorcontrib>Lee, Moon Sung</creatorcontrib><creatorcontrib>Hwang, Seung Duk</creatorcontrib><creatorcontrib>Shim, Chan Sup</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of gastroenterology & hepatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Yun Soo</au><au>Kim, Sung II</au><au>Hwang, Seong Gyu</au><au>Kim, Jin Oh</au><au>Cho, Joo Young</au><au>Lee, Joon Seong</au><au>Lee, Moon Sung</au><au>Hwang, Seung Duk</au><au>Shim, Chan Sup</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diversity of core promoter mutations in immune clearance phase of chronic HBV infection</atitle><jtitle>European journal of gastroenterology & hepatology</jtitle><addtitle>Eur J Gastroenterol Hepatol</addtitle><date>1999-08</date><risdate>1999</risdate><volume>11</volume><issue>8</issue><spage>821</spage><epage>826</epage><pages>821-826</pages><issn>0954-691X</issn><eissn>1473-5687</eissn><abstract>Transcription of HBV (hepatitis B virus) pre-core and pre-genomic mRNAs is controlled by core promoter. Therefore, mutations in the core promoter region might change the activity of liver diseases through an altered transcriptional level of the mRNA. The present study was carried out to determine the diversity of HBV core promoter sequences in chronic HBV carriers.
DNA sequences in the core promoter region were determined after cloning the PCR product. Two groups of chronic HBV carriers with HBeAg, including five cases of asymptomatic carriers (ASCs, 21 clones) and eight with chronic hepatitis (CH, 50 clones) were studied.
Mutations in the core promoter were found in three out of the ASCs (11 clones), and in all eight cases in the CH group (48 clones). While mutations at nucleotide 1762 (A-->T) and 1764(G-->A) were not found in ASC, mutations at the same positions were found in all the cases of CH group (40 clones) (P=0.003). Diverse patterns of mutations in the core promoter were observed in each patient in the CH group.
Further studies are needed to determine whether the diversity of HBV core promoter mutations has clinical significance such as the seroconversion of HBeAg to anti-HBe.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott Williams & Wilkins, Inc</pub><pmid>10514111</pmid><doi>10.1097/00042737-199908000-00002</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0954-691X |
| ispartof | European journal of gastroenterology & hepatology, 1999-08, Vol.11 (8), p.821-826 |
| issn | 0954-691X 1473-5687 |
| language | eng |
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| source | MEDLINE; Journals@Ovid Complete |
| subjects | Adult Base Sequence Biological and medical sciences Carrier State - immunology Carrier State - virology Child DNA Mutational Analysis DNA, Viral - genetics Female Hepatitis B Antibodies - blood Hepatitis B e Antigens - blood Hepatitis B virus - genetics Hepatitis B virus - isolation & purification Hepatitis B, Chronic - immunology Hepatitis B, Chronic - virology Human viral diseases Humans Infectious diseases Male Medical sciences Middle Aged Molecular Sequence Data Mutation Polymerase Chain Reaction Promoter Regions, Genetic - genetics Tropical medicine Viral Core Proteins - genetics Viral diseases Viral hepatitis |
| title | Diversity of core promoter mutations in immune clearance phase of chronic HBV infection |
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