Improved osmotolerance of recombinant Escherichia coli by de novo glycine betaine biosynthesis

The genes from the extreme halophile Ecto-thiorhodospira halochloris encoding the biosynthesis of glycine betaine from glycine were cloned into Escherichia coli. The accumulation of glycine betaine and its effect on osmotolerance of the cells were studied. In mineral medium with NaCl concentrations...

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Veröffentlicht in:	Applied microbiology and biotechnology 2001-03, Vol.55 (2), p.214-218
Hauptverfasser:	VON WEYMARN, N, NYYSSÖLÄ, A, REINIKAINEN, T, LEISOLA, M, OJAMO, H
Format:	Artikel
Sprache:	eng
Schlagworte:	Accumulation Acetates - metabolism Bacteria Bacterial Proteins betaine Betaine - metabolism Betaine - pharmacology Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biosynthesis Biotechnology Cloning, Molecular Culture Media E coli Ectothiorhodospira - enzymology Ectothiorhodospira - genetics Ectothiorhodospira halochloris Escherichia coli Escherichia coli - genetics Escherichia coli - growth & development Escherichia coli - metabolism Escherichia coli - physiology Fundamental and applied biological sciences. Psychology Genetics Glycine N-Methyltransferase Isopropyl Thiogalactoside - pharmacology Methyltransferases - genetics Methyltransferases - metabolism Microbiology Mission oriented research Osmolar Concentration Osmosis Recombinant Proteins - metabolism Sodium chloride Sodium Chloride - pharmacology Sucrose - pharmacology Temperature Yeast
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