Improved osmotolerance of recombinant Escherichia coli by de novo glycine betaine biosynthesis
The genes from the extreme halophile Ecto-thiorhodospira halochloris encoding the biosynthesis of glycine betaine from glycine were cloned into Escherichia coli. The accumulation of glycine betaine and its effect on osmotolerance of the cells were studied. In mineral medium with NaCl concentrations...
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| Veröffentlicht in: | Applied microbiology and biotechnology 2001-03, Vol.55 (2), p.214-218 |
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| creator | VON WEYMARN, N NYYSSÖLÄ, A REINIKAINEN, T LEISOLA, M OJAMO, H |
| description | The genes from the extreme halophile Ecto-thiorhodospira halochloris encoding the biosynthesis of glycine betaine from glycine were cloned into Escherichia coli. The accumulation of glycine betaine and its effect on osmotolerance of the cells were studied. In mineral medium with NaCl concentrations from 0.15 to 0.5 M, the accumulation of both endogenously synthesized and exogenously provided glycine betaine stimulated the growth of E. coli. The intracellular levels of glycine betaine and the cellular yields were clearly higher for cells receiving glycine betaine exogenously than for cells synthesizing it. The lower level of glycine betaine accumulation in cells synthesizing it is most likely a consequence of the limited availability of precursors (e.g. S-adenosylmethionine) rather than the result of a low expression level of the genes. Glycine betaine also stimulated the growth of E. coli and decreased acetate formation in mineral medium with high sucrose concentrations (up to 200 g.l(-1)). |
| doi_str_mv | 10.1007/s002530000515 |
| format | Article |
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The accumulation of glycine betaine and its effect on osmotolerance of the cells were studied. In mineral medium with NaCl concentrations from 0.15 to 0.5 M, the accumulation of both endogenously synthesized and exogenously provided glycine betaine stimulated the growth of E. coli. The intracellular levels of glycine betaine and the cellular yields were clearly higher for cells receiving glycine betaine exogenously than for cells synthesizing it. The lower level of glycine betaine accumulation in cells synthesizing it is most likely a consequence of the limited availability of precursors (e.g. S-adenosylmethionine) rather than the result of a low expression level of the genes. Glycine betaine also stimulated the growth of E. coli and decreased acetate formation in mineral medium with high sucrose concentrations (up to 200 g.l(-1)).</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s002530000515</identifier><identifier>PMID: 11330717</identifier><identifier>CODEN: AMBIDG</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Accumulation ; Acetates - metabolism ; Bacteria ; Bacterial Proteins ; betaine ; Betaine - metabolism ; Betaine - pharmacology ; Biological and medical sciences ; Biology of microorganisms of confirmed or potential industrial interest ; Biosynthesis ; Biotechnology ; Cloning, Molecular ; Culture Media ; E coli ; Ectothiorhodospira - enzymology ; Ectothiorhodospira - genetics ; Ectothiorhodospira halochloris ; Escherichia coli ; Escherichia coli - genetics ; Escherichia coli - growth & development ; Escherichia coli - metabolism ; Escherichia coli - physiology ; Fundamental and applied biological sciences. Psychology ; Genetics ; Glycine N-Methyltransferase ; Isopropyl Thiogalactoside - pharmacology ; Methyltransferases - genetics ; Methyltransferases - metabolism ; Microbiology ; Mission oriented research ; Osmolar Concentration ; Osmosis ; Recombinant Proteins - metabolism ; Sodium chloride ; Sodium Chloride - pharmacology ; Sucrose - pharmacology ; Temperature ; Yeast</subject><ispartof>Applied microbiology and biotechnology, 2001-03, Vol.55 (2), p.214-218</ispartof><rights>2001 INIST-CNRS</rights><rights>Springer-Verlag 2000</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-b18bfcf5ea94f58623b95a5b5a8efda4dd912b10827f1af4b3db2a9e11e384e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1032409$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11330717$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>VON WEYMARN, N</creatorcontrib><creatorcontrib>NYYSSÖLÄ, A</creatorcontrib><creatorcontrib>REINIKAINEN, T</creatorcontrib><creatorcontrib>LEISOLA, M</creatorcontrib><creatorcontrib>OJAMO, H</creatorcontrib><title>Improved osmotolerance of recombinant Escherichia coli by de novo glycine betaine biosynthesis</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><description>The genes from the extreme halophile Ecto-thiorhodospira halochloris encoding the biosynthesis of glycine betaine from glycine were cloned into Escherichia coli. The accumulation of glycine betaine and its effect on osmotolerance of the cells were studied. In mineral medium with NaCl concentrations from 0.15 to 0.5 M, the accumulation of both endogenously synthesized and exogenously provided glycine betaine stimulated the growth of E. coli. The intracellular levels of glycine betaine and the cellular yields were clearly higher for cells receiving glycine betaine exogenously than for cells synthesizing it. The lower level of glycine betaine accumulation in cells synthesizing it is most likely a consequence of the limited availability of precursors (e.g. S-adenosylmethionine) rather than the result of a low expression level of the genes. Glycine betaine also stimulated the growth of E. coli and decreased acetate formation in mineral medium with high sucrose concentrations (up to 200 g.l(-1)).</description><subject>Accumulation</subject><subject>Acetates - metabolism</subject><subject>Bacteria</subject><subject>Bacterial Proteins</subject><subject>betaine</subject><subject>Betaine - metabolism</subject><subject>Betaine - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Biology of microorganisms of confirmed or potential industrial interest</subject><subject>Biosynthesis</subject><subject>Biotechnology</subject><subject>Cloning, Molecular</subject><subject>Culture Media</subject><subject>E coli</subject><subject>Ectothiorhodospira - enzymology</subject><subject>Ectothiorhodospira - genetics</subject><subject>Ectothiorhodospira halochloris</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - growth & development</subject><subject>Escherichia coli - metabolism</subject><subject>Escherichia coli - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics</subject><subject>Glycine N-Methyltransferase</subject><subject>Isopropyl Thiogalactoside - pharmacology</subject><subject>Methyltransferases - genetics</subject><subject>Methyltransferases - metabolism</subject><subject>Microbiology</subject><subject>Mission oriented research</subject><subject>Osmolar Concentration</subject><subject>Osmosis</subject><subject>Recombinant Proteins - metabolism</subject><subject>Sodium chloride</subject><subject>Sodium Chloride - pharmacology</subject><subject>Sucrose - pharmacology</subject><subject>Temperature</subject><subject>Yeast</subject><issn>0175-7598</issn><issn>1432-0614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqF0U1r3DAQBmBRErqbtMdcgwilN6cafdjysSzbJhDIJecYSR51tdhWKnkD---jbRaa9FJd5vLMC6OXkAtg18BY8y0zxpVg5SlQH8gSpOAVq0GekCWDRlWNavWCnOW8ZQy4ruuPZAEgBGugWZLH2_EpxWfsacxjnOOAyUwOafQ0oYujDZOZZrrOboMpuE0w1MUhULunPdIpPkf6a9i7MCG1OJs_M8S8n-YN5pA_kVNvhoyfj_OcPPxYP6xuqrv7n7er73eVkyDmyoK23nmFppVe6ZoL2yqjrDIafW9k37fALTDNGw_GSyt6y02LACi0RHFOvr7Gllt-7zDP3Riyw2EwE8Zd7pqyybVk_4WgoS7fCgVe_QO3cZemckOntawVA2gLql6RSzHnhL57SmE0ad8B6w7tdO_aKf7yGLqzI_Z_9bGOAr4cgcnODP7QRchvUgWXrBUvyG-XTg</recordid><startdate>20010301</startdate><enddate>20010301</enddate><creator>VON WEYMARN, N</creator><creator>NYYSSÖLÄ, A</creator><creator>REINIKAINEN, T</creator><creator>LEISOLA, M</creator><creator>OJAMO, H</creator><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>LK8</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7QO</scope><scope>7X8</scope></search><sort><creationdate>20010301</creationdate><title>Improved osmotolerance of recombinant Escherichia coli by de novo glycine betaine biosynthesis</title><author>VON WEYMARN, N ; NYYSSÖLÄ, A ; REINIKAINEN, T ; LEISOLA, M ; OJAMO, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-b18bfcf5ea94f58623b95a5b5a8efda4dd912b10827f1af4b3db2a9e11e384e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Accumulation</topic><topic>Acetates - metabolism</topic><topic>Bacteria</topic><topic>Bacterial Proteins</topic><topic>betaine</topic><topic>Betaine - metabolism</topic><topic>Betaine - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Biology of microorganisms of confirmed or potential industrial interest</topic><topic>Biosynthesis</topic><topic>Biotechnology</topic><topic>Cloning, Molecular</topic><topic>Culture Media</topic><topic>E coli</topic><topic>Ectothiorhodospira - enzymology</topic><topic>Ectothiorhodospira - genetics</topic><topic>Ectothiorhodospira halochloris</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - growth & development</topic><topic>Escherichia coli - metabolism</topic><topic>Escherichia coli - physiology</topic><topic>Fundamental and applied biological sciences. 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Academic</collection><jtitle>Applied microbiology and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>VON WEYMARN, N</au><au>NYYSSÖLÄ, A</au><au>REINIKAINEN, T</au><au>LEISOLA, M</au><au>OJAMO, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improved osmotolerance of recombinant Escherichia coli by de novo glycine betaine biosynthesis</atitle><jtitle>Applied microbiology and biotechnology</jtitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>2001-03-01</date><risdate>2001</risdate><volume>55</volume><issue>2</issue><spage>214</spage><epage>218</epage><pages>214-218</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><coden>AMBIDG</coden><abstract>The genes from the extreme halophile Ecto-thiorhodospira halochloris encoding the biosynthesis of glycine betaine from glycine were cloned into Escherichia coli. The accumulation of glycine betaine and its effect on osmotolerance of the cells were studied. In mineral medium with NaCl concentrations from 0.15 to 0.5 M, the accumulation of both endogenously synthesized and exogenously provided glycine betaine stimulated the growth of E. coli. The intracellular levels of glycine betaine and the cellular yields were clearly higher for cells receiving glycine betaine exogenously than for cells synthesizing it. The lower level of glycine betaine accumulation in cells synthesizing it is most likely a consequence of the limited availability of precursors (e.g. S-adenosylmethionine) rather than the result of a low expression level of the genes. Glycine betaine also stimulated the growth of E. coli and decreased acetate formation in mineral medium with high sucrose concentrations (up to 200 g.l(-1)).</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>11330717</pmid><doi>10.1007/s002530000515</doi><tpages>5</tpages></addata></record> |
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| subjects | Accumulation Acetates - metabolism Bacteria Bacterial Proteins betaine Betaine - metabolism Betaine - pharmacology Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biosynthesis Biotechnology Cloning, Molecular Culture Media E coli Ectothiorhodospira - enzymology Ectothiorhodospira - genetics Ectothiorhodospira halochloris Escherichia coli Escherichia coli - genetics Escherichia coli - growth & development Escherichia coli - metabolism Escherichia coli - physiology Fundamental and applied biological sciences. Psychology Genetics Glycine N-Methyltransferase Isopropyl Thiogalactoside - pharmacology Methyltransferases - genetics Methyltransferases - metabolism Microbiology Mission oriented research Osmolar Concentration Osmosis Recombinant Proteins - metabolism Sodium chloride Sodium Chloride - pharmacology Sucrose - pharmacology Temperature Yeast |
| title | Improved osmotolerance of recombinant Escherichia coli by de novo glycine betaine biosynthesis |
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