Mineralocorticoid Receptors in the Mammalian Olfactory Mucosa
Mineralocorticoid hormones regulate secretion and absorption in a wide variety of epithelial tissues, although specific mechanisms in the olfactory mucosa are currently unknown. Utilizing reverse transcription—polymerase chain reaction (RT-PCR) analysis, we have demonstrated the expression of minera...
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| Veröffentlicht in: | Annals of otology, rhinology & laryngology rhinology & laryngology, 1999-10, Vol.108 (10), p.974-981 |
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| container_title | Annals of otology, rhinology & laryngology |
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| creator | Robinson, Alan M. Kern, Robert C. Krozowski, Zygmunt S. Foster, James D. Pitovski, Dimitri Z. |
| description | Mineralocorticoid hormones regulate secretion and absorption in a wide variety of epithelial tissues, although specific mechanisms in the olfactory mucosa are currently unknown. Utilizing reverse transcription—polymerase chain reaction (RT-PCR) analysis, we have demonstrated the expression of mineralocorticoid (type I) receptor messenger RNA in the rodent olfactory mucosa. Amplification products of predicted size were obtained with nucleotide sequences corresponding to respective mineralocorticoid receptor (MR) kidney transcripts. Immunocytochemistry, using an antibody with known specificity for MRs, was then utilized in order to localize the cellular site(s) of MR protein expression in the olfactory mucosa. The highest levels of MR immunoreactivity were localized to the supranuclear region of sustentacular cells, as well as the acinar cells of the Bowman's glands. The respiratory regions of the nasal cavity were devoid of appreciable MR immunoreactivity. This study demonstrates both MR transcript and protein expression in the olfactory mucosa. We hypothesize that the mineralocorticoid hormones may have a role in modulation of olfactory secretion and/or sensory transduction in the peripheral olfactory system. |
| doi_str_mv | 10.1177/000348949910801009 |
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Utilizing reverse transcription—polymerase chain reaction (RT-PCR) analysis, we have demonstrated the expression of mineralocorticoid (type I) receptor messenger RNA in the rodent olfactory mucosa. Amplification products of predicted size were obtained with nucleotide sequences corresponding to respective mineralocorticoid receptor (MR) kidney transcripts. Immunocytochemistry, using an antibody with known specificity for MRs, was then utilized in order to localize the cellular site(s) of MR protein expression in the olfactory mucosa. The highest levels of MR immunoreactivity were localized to the supranuclear region of sustentacular cells, as well as the acinar cells of the Bowman's glands. The respiratory regions of the nasal cavity were devoid of appreciable MR immunoreactivity. This study demonstrates both MR transcript and protein expression in the olfactory mucosa. We hypothesize that the mineralocorticoid hormones may have a role in modulation of olfactory secretion and/or sensory transduction in the peripheral olfactory system.</description><identifier>ISSN: 0003-4894</identifier><identifier>EISSN: 1943-572X</identifier><identifier>DOI: 10.1177/000348949910801009</identifier><identifier>PMID: 10526853</identifier><identifier>CODEN: AORHA2</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Animals ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; Gene Expression - genetics ; Guinea Pigs ; Mice ; Olfactory Mucosa - cytology ; Olfactory Mucosa - metabolism ; Olfactory system and olfaction. Gustatory system and gustation ; Rats ; Receptors, Mineralocorticoid - metabolism ; Vertebrates: nervous system and sense organs</subject><ispartof>Annals of otology, rhinology & laryngology, 1999-10, Vol.108 (10), p.974-981</ispartof><rights>1999 SAGE Publications</rights><rights>1999 INIST-CNRS</rights><rights>Copyright Annals Publishing Company Oct 1999</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c461t-2bc884903a4fd57f44318a03c222c13e863a16bc0456714c7739a170c29f93fc3</citedby><cites>FETCH-LOGICAL-c461t-2bc884903a4fd57f44318a03c222c13e863a16bc0456714c7739a170c29f93fc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/000348949910801009$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/000348949910801009$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>315,782,786,21826,27931,27932,43628,43629</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1981527$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10526853$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Robinson, Alan M.</creatorcontrib><creatorcontrib>Kern, Robert C.</creatorcontrib><creatorcontrib>Krozowski, Zygmunt S.</creatorcontrib><creatorcontrib>Foster, James D.</creatorcontrib><creatorcontrib>Pitovski, Dimitri Z.</creatorcontrib><title>Mineralocorticoid Receptors in the Mammalian Olfactory Mucosa</title><title>Annals of otology, rhinology & laryngology</title><addtitle>Ann Otol Rhinol Laryngol</addtitle><description>Mineralocorticoid hormones regulate secretion and absorption in a wide variety of epithelial tissues, although specific mechanisms in the olfactory mucosa are currently unknown. Utilizing reverse transcription—polymerase chain reaction (RT-PCR) analysis, we have demonstrated the expression of mineralocorticoid (type I) receptor messenger RNA in the rodent olfactory mucosa. Amplification products of predicted size were obtained with nucleotide sequences corresponding to respective mineralocorticoid receptor (MR) kidney transcripts. Immunocytochemistry, using an antibody with known specificity for MRs, was then utilized in order to localize the cellular site(s) of MR protein expression in the olfactory mucosa. The highest levels of MR immunoreactivity were localized to the supranuclear region of sustentacular cells, as well as the acinar cells of the Bowman's glands. The respiratory regions of the nasal cavity were devoid of appreciable MR immunoreactivity. This study demonstrates both MR transcript and protein expression in the olfactory mucosa. We hypothesize that the mineralocorticoid hormones may have a role in modulation of olfactory secretion and/or sensory transduction in the peripheral olfactory system.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression - genetics</subject><subject>Guinea Pigs</subject><subject>Mice</subject><subject>Olfactory Mucosa - cytology</subject><subject>Olfactory Mucosa - metabolism</subject><subject>Olfactory system and olfaction. Gustatory system and gustation</subject><subject>Rats</subject><subject>Receptors, Mineralocorticoid - metabolism</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0003-4894</issn><issn>1943-572X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp90EtLxDAQB_Agiq6PL-BBioi3upkkbZKDB1l8gcuCKHgrs7OpVtpmTdqD394uu6AoeApDfvPgz9gx8AsArcecc6mMVdYCNxw4t1tsBFbJNNPiZZuNViBdiT22H-P7UKqMi122BzwTucnkiF1Oq9YFrD350FXkq0Xy6MgtOx9iUrVJ9-aSKTYN1hW2yawukYavz2Tak494yHZKrKM72rwH7Pnm-mlylz7Mbu8nVw8pqRy6VMzJGGW5RFUuMl0qJcEglySEIJDO5BIhn9NwXq5BkdbSImhOwpZWliQP2Pl67jL4j97FrmiqSK6usXW-j4XmRohMwgBPf8F334d2uK0QoI02udIDEmtEwccYXFksQ9Vg-CyAF6tki7_JDk0nm8n9vHGLHy3rKAdwtgEYCesyYEtV_HbWQCZWy8drFvHVfV_3z-YvLaSK_g</recordid><startdate>19991001</startdate><enddate>19991001</enddate><creator>Robinson, Alan M.</creator><creator>Kern, Robert C.</creator><creator>Krozowski, Zygmunt S.</creator><creator>Foster, James D.</creator><creator>Pitovski, Dimitri Z.</creator><general>SAGE Publications</general><general>Annals Publishing Compagny</general><general>SAGE PUBLICATIONS, INC</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>S0X</scope><scope>7X8</scope><scope>8BM</scope></search><sort><creationdate>19991001</creationdate><title>Mineralocorticoid Receptors in the Mammalian Olfactory Mucosa</title><author>Robinson, Alan M. ; Kern, Robert C. ; Krozowski, Zygmunt S. ; Foster, James D. ; Pitovski, Dimitri Z.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c461t-2bc884903a4fd57f44318a03c222c13e863a16bc0456714c7739a170c29f93fc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. 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Gustatory system and gustation</topic><topic>Rats</topic><topic>Receptors, Mineralocorticoid - metabolism</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Robinson, Alan M.</creatorcontrib><creatorcontrib>Kern, Robert C.</creatorcontrib><creatorcontrib>Krozowski, Zygmunt S.</creatorcontrib><creatorcontrib>Foster, James D.</creatorcontrib><creatorcontrib>Pitovski, Dimitri Z.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database (ProQuest)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><collection>MEDLINE - Academic</collection><collection>ComDisDome</collection><jtitle>Annals of otology, rhinology & laryngology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Robinson, Alan M.</au><au>Kern, Robert C.</au><au>Krozowski, Zygmunt S.</au><au>Foster, James D.</au><au>Pitovski, Dimitri Z.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mineralocorticoid Receptors in the Mammalian Olfactory Mucosa</atitle><jtitle>Annals of otology, rhinology & laryngology</jtitle><addtitle>Ann Otol Rhinol Laryngol</addtitle><date>1999-10-01</date><risdate>1999</risdate><volume>108</volume><issue>10</issue><spage>974</spage><epage>981</epage><pages>974-981</pages><issn>0003-4894</issn><eissn>1943-572X</eissn><coden>AORHA2</coden><abstract>Mineralocorticoid hormones regulate secretion and absorption in a wide variety of epithelial tissues, although specific mechanisms in the olfactory mucosa are currently unknown. Utilizing reverse transcription—polymerase chain reaction (RT-PCR) analysis, we have demonstrated the expression of mineralocorticoid (type I) receptor messenger RNA in the rodent olfactory mucosa. Amplification products of predicted size were obtained with nucleotide sequences corresponding to respective mineralocorticoid receptor (MR) kidney transcripts. Immunocytochemistry, using an antibody with known specificity for MRs, was then utilized in order to localize the cellular site(s) of MR protein expression in the olfactory mucosa. The highest levels of MR immunoreactivity were localized to the supranuclear region of sustentacular cells, as well as the acinar cells of the Bowman's glands. The respiratory regions of the nasal cavity were devoid of appreciable MR immunoreactivity. This study demonstrates both MR transcript and protein expression in the olfactory mucosa. 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| subjects | Animals Biological and medical sciences Fundamental and applied biological sciences. Psychology Gene Expression - genetics Guinea Pigs Mice Olfactory Mucosa - cytology Olfactory Mucosa - metabolism Olfactory system and olfaction. Gustatory system and gustation Rats Receptors, Mineralocorticoid - metabolism Vertebrates: nervous system and sense organs |
| title | Mineralocorticoid Receptors in the Mammalian Olfactory Mucosa |
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