Aneuploidy frequencies in semen fractions from ten oligoasthenoteratozoospermic patients donating sperm for intracytoplasmic sperm injection
Objective: To determine aneuploidy frequencies in pellet and swim-up semen fractions from 10 infertile men with severe oligoasthenoteratozoospermia (OAT) who were donating sperm for intracytoplasmic sperm injection and to determine whether the swim-up isolation method would successfully separate ane...
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| Veröffentlicht in: | Fertility and sterility 1999-09, Vol.72 (3), p.472-478 |
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| container_title | Fertility and sterility |
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| creator | Pfeffer, Jerome Pang, Myung-Geol Hoegerman, Stanton F Osgood, Christopher J Stacey, M.W Mayer, Jacob Oehninger, Sergio Kearns, William G |
| description | Objective: To determine aneuploidy frequencies in pellet and swim-up semen fractions from 10 infertile men with severe oligoasthenoteratozoospermia (OAT) who were donating sperm for intracytoplasmic sperm injection and to determine whether the swim-up isolation method would successfully separate aneuploid from haploid sperm.
Design: Prospective study.
Setting: Infertility clinic and molecular genetics laboratory.
Patient(s): Ten patients with severe OAT.
Intervention(s): Cytogenetic analyses by fluorescence in situ hybridization to determine aneuploidy frequencies for chromosomes 1, 13, 18, 21, X, and Y in sperm from swim-up and pellet fractions.
Main Outcome Measure(s): Gametic aneuploidy was scored in sperm fractions separated by the swim-up technique and clinical results after intracytoplasmic sperm injection were tabulated.
Result(s): In all cases, chromosome aneuploidy levels in patients were significantly greater than in controls. The type and percentage of aneuploid sperm for all patients with OAT found in both swim-up and pellet fractions were not different, with the exception of diploid sperm, which remained in the pellet fraction. After ET, 2 (20%) of 10 couples achieved successful pregnancies.
Conclusion(s): The data show significantly higher rates of diploidy, autosomal disomy and nullisomy, sex chromosome disomy and nullisomy, and total aneuploidy in sperm from all separated fractions obtained from all patients with OAT versus controls. This patient population with OAT may be at increased risk of producing aneuploid offspring. |
| doi_str_mv | 10.1016/S0015-0282(99)00279-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70822060</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0015028299002794</els_id><sourcerecordid>70822060</sourcerecordid><originalsourceid>FETCH-LOGICAL-c489t-92d58ea236299be3faf00d03c1956abaedd450e4d400b2df74f188fca2eee9e83</originalsourceid><addsrcrecordid>eNqFkc9u1DAQxi0EotvCI4ByQKgcAmMnTuJTVVX8kypxAM6W154UV4kdPF6k5Rl4aLybFXDj5JHnN9_Y38fYMw6vOfDuzWcALmsQg7hU6hWA6FXdPmAbLmVXy042D9nmD3LGzonuAaDjvXjMzjhIrjquNuzXdcDdMkXv9tWY8PsOg_VIlQ8V4YyhXBqbfQxUqjhXuVzFyd9FQ_kbhpgxmRx_xkgLptnbajHZY8hUuRhKGe6qY6caYyqiuajtc1wmQwd4bflwj8cdT9ij0UyET0_nBfv67u2Xmw_17af3H2-ub2vbDirXSjg5oBFNJ5TaYjOaEcBBY7mSndkadK6VgK1rAbbCjX078mEYrRGIqHBoLtjLVXdJsfyYsp49WZwmEzDuSPcwCAEdFFCuoE2RKOGol-Rnk_aagz7EoI8x6IPHWil9jEG3Ze75acFuO6P7Z2r1vQAvToAha6ZicrGd_nK8HxQcdK5WDIsbPzwmTSWdYNH5VCzTLvr_vOQ3p3up6g</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>70822060</pqid></control><display><type>article</type><title>Aneuploidy frequencies in semen fractions from ten oligoasthenoteratozoospermic patients donating sperm for intracytoplasmic sperm injection</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Pfeffer, Jerome ; Pang, Myung-Geol ; Hoegerman, Stanton F ; Osgood, Christopher J ; Stacey, M.W ; Mayer, Jacob ; Oehninger, Sergio ; Kearns, William G</creator><creatorcontrib>Pfeffer, Jerome ; Pang, Myung-Geol ; Hoegerman, Stanton F ; Osgood, Christopher J ; Stacey, M.W ; Mayer, Jacob ; Oehninger, Sergio ; Kearns, William G</creatorcontrib><description>Objective: To determine aneuploidy frequencies in pellet and swim-up semen fractions from 10 infertile men with severe oligoasthenoteratozoospermia (OAT) who were donating sperm for intracytoplasmic sperm injection and to determine whether the swim-up isolation method would successfully separate aneuploid from haploid sperm.
Design: Prospective study.
Setting: Infertility clinic and molecular genetics laboratory.
Patient(s): Ten patients with severe OAT.
Intervention(s): Cytogenetic analyses by fluorescence in situ hybridization to determine aneuploidy frequencies for chromosomes 1, 13, 18, 21, X, and Y in sperm from swim-up and pellet fractions.
Main Outcome Measure(s): Gametic aneuploidy was scored in sperm fractions separated by the swim-up technique and clinical results after intracytoplasmic sperm injection were tabulated.
Result(s): In all cases, chromosome aneuploidy levels in patients were significantly greater than in controls. The type and percentage of aneuploid sperm for all patients with OAT found in both swim-up and pellet fractions were not different, with the exception of diploid sperm, which remained in the pellet fraction. After ET, 2 (20%) of 10 couples achieved successful pregnancies.
Conclusion(s): The data show significantly higher rates of diploidy, autosomal disomy and nullisomy, sex chromosome disomy and nullisomy, and total aneuploidy in sperm from all separated fractions obtained from all patients with OAT versus controls. This patient population with OAT may be at increased risk of producing aneuploid offspring.</description><identifier>ISSN: 0015-0282</identifier><identifier>EISSN: 1556-5653</identifier><identifier>DOI: 10.1016/S0015-0282(99)00279-4</identifier><identifier>PMID: 10519619</identifier><identifier>CODEN: FESTAS</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Aneuploidy ; Biological and medical sciences ; Diploidy ; DNA - analysis ; Embryo Transfer ; Female ; Fertilization in Vitro - methods ; FISH ; Fluorescence in situ hybridization ; Gynecology. Andrology. Obstetrics ; Humans ; ICSI ; In Situ Hybridization, Fluorescence ; infertility ; intracytoplasmic sperm injection ; Male ; Male genital diseases ; Medical sciences ; Microinjections ; Non tumoral diseases ; OAT ; oligoasthenoteratozoospermia ; Oligospermia - genetics ; Oocytes - ultrastructure ; Pregnancy ; Prospective Studies ; Risk Factors ; Semen - chemistry ; Sperm Count ; Sperm Motility ; Spermatozoa - abnormalities ; swim-up separation ; Tissue Donors</subject><ispartof>Fertility and sterility, 1999-09, Vol.72 (3), p.472-478</ispartof><rights>1999 American Society for Reproductive Medicine</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c489t-92d58ea236299be3faf00d03c1956abaedd450e4d400b2df74f188fca2eee9e83</citedby><cites>FETCH-LOGICAL-c489t-92d58ea236299be3faf00d03c1956abaedd450e4d400b2df74f188fca2eee9e83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0015028299002794$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1178904$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10519619$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pfeffer, Jerome</creatorcontrib><creatorcontrib>Pang, Myung-Geol</creatorcontrib><creatorcontrib>Hoegerman, Stanton F</creatorcontrib><creatorcontrib>Osgood, Christopher J</creatorcontrib><creatorcontrib>Stacey, M.W</creatorcontrib><creatorcontrib>Mayer, Jacob</creatorcontrib><creatorcontrib>Oehninger, Sergio</creatorcontrib><creatorcontrib>Kearns, William G</creatorcontrib><title>Aneuploidy frequencies in semen fractions from ten oligoasthenoteratozoospermic patients donating sperm for intracytoplasmic sperm injection</title><title>Fertility and sterility</title><addtitle>Fertil Steril</addtitle><description>Objective: To determine aneuploidy frequencies in pellet and swim-up semen fractions from 10 infertile men with severe oligoasthenoteratozoospermia (OAT) who were donating sperm for intracytoplasmic sperm injection and to determine whether the swim-up isolation method would successfully separate aneuploid from haploid sperm.
Design: Prospective study.
Setting: Infertility clinic and molecular genetics laboratory.
Patient(s): Ten patients with severe OAT.
Intervention(s): Cytogenetic analyses by fluorescence in situ hybridization to determine aneuploidy frequencies for chromosomes 1, 13, 18, 21, X, and Y in sperm from swim-up and pellet fractions.
Main Outcome Measure(s): Gametic aneuploidy was scored in sperm fractions separated by the swim-up technique and clinical results after intracytoplasmic sperm injection were tabulated.
Result(s): In all cases, chromosome aneuploidy levels in patients were significantly greater than in controls. The type and percentage of aneuploid sperm for all patients with OAT found in both swim-up and pellet fractions were not different, with the exception of diploid sperm, which remained in the pellet fraction. After ET, 2 (20%) of 10 couples achieved successful pregnancies.
Conclusion(s): The data show significantly higher rates of diploidy, autosomal disomy and nullisomy, sex chromosome disomy and nullisomy, and total aneuploidy in sperm from all separated fractions obtained from all patients with OAT versus controls. This patient population with OAT may be at increased risk of producing aneuploid offspring.</description><subject>Aneuploidy</subject><subject>Biological and medical sciences</subject><subject>Diploidy</subject><subject>DNA - analysis</subject><subject>Embryo Transfer</subject><subject>Female</subject><subject>Fertilization in Vitro - methods</subject><subject>FISH</subject><subject>Fluorescence in situ hybridization</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>ICSI</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>infertility</subject><subject>intracytoplasmic sperm injection</subject><subject>Male</subject><subject>Male genital diseases</subject><subject>Medical sciences</subject><subject>Microinjections</subject><subject>Non tumoral diseases</subject><subject>OAT</subject><subject>oligoasthenoteratozoospermia</subject><subject>Oligospermia - genetics</subject><subject>Oocytes - ultrastructure</subject><subject>Pregnancy</subject><subject>Prospective Studies</subject><subject>Risk Factors</subject><subject>Semen - chemistry</subject><subject>Sperm Count</subject><subject>Sperm Motility</subject><subject>Spermatozoa - abnormalities</subject><subject>swim-up separation</subject><subject>Tissue Donors</subject><issn>0015-0282</issn><issn>1556-5653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9u1DAQxi0EotvCI4ByQKgcAmMnTuJTVVX8kypxAM6W154UV4kdPF6k5Rl4aLybFXDj5JHnN9_Y38fYMw6vOfDuzWcALmsQg7hU6hWA6FXdPmAbLmVXy042D9nmD3LGzonuAaDjvXjMzjhIrjquNuzXdcDdMkXv9tWY8PsOg_VIlQ8V4YyhXBqbfQxUqjhXuVzFyd9FQ_kbhpgxmRx_xkgLptnbajHZY8hUuRhKGe6qY6caYyqiuajtc1wmQwd4bflwj8cdT9ij0UyET0_nBfv67u2Xmw_17af3H2-ub2vbDirXSjg5oBFNJ5TaYjOaEcBBY7mSndkadK6VgK1rAbbCjX078mEYrRGIqHBoLtjLVXdJsfyYsp49WZwmEzDuSPcwCAEdFFCuoE2RKOGol-Rnk_aagz7EoI8x6IPHWil9jEG3Ze75acFuO6P7Z2r1vQAvToAha6ZicrGd_nK8HxQcdK5WDIsbPzwmTSWdYNH5VCzTLvr_vOQ3p3up6g</recordid><startdate>19990901</startdate><enddate>19990901</enddate><creator>Pfeffer, Jerome</creator><creator>Pang, Myung-Geol</creator><creator>Hoegerman, Stanton F</creator><creator>Osgood, Christopher J</creator><creator>Stacey, M.W</creator><creator>Mayer, Jacob</creator><creator>Oehninger, Sergio</creator><creator>Kearns, William G</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990901</creationdate><title>Aneuploidy frequencies in semen fractions from ten oligoasthenoteratozoospermic patients donating sperm for intracytoplasmic sperm injection</title><author>Pfeffer, Jerome ; Pang, Myung-Geol ; Hoegerman, Stanton F ; Osgood, Christopher J ; Stacey, M.W ; Mayer, Jacob ; Oehninger, Sergio ; Kearns, William G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c489t-92d58ea236299be3faf00d03c1956abaedd450e4d400b2df74f188fca2eee9e83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Aneuploidy</topic><topic>Biological and medical sciences</topic><topic>Diploidy</topic><topic>DNA - analysis</topic><topic>Embryo Transfer</topic><topic>Female</topic><topic>Fertilization in Vitro - methods</topic><topic>FISH</topic><topic>Fluorescence in situ hybridization</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>ICSI</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>infertility</topic><topic>intracytoplasmic sperm injection</topic><topic>Male</topic><topic>Male genital diseases</topic><topic>Medical sciences</topic><topic>Microinjections</topic><topic>Non tumoral diseases</topic><topic>OAT</topic><topic>oligoasthenoteratozoospermia</topic><topic>Oligospermia - genetics</topic><topic>Oocytes - ultrastructure</topic><topic>Pregnancy</topic><topic>Prospective Studies</topic><topic>Risk Factors</topic><topic>Semen - chemistry</topic><topic>Sperm Count</topic><topic>Sperm Motility</topic><topic>Spermatozoa - abnormalities</topic><topic>swim-up separation</topic><topic>Tissue Donors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pfeffer, Jerome</creatorcontrib><creatorcontrib>Pang, Myung-Geol</creatorcontrib><creatorcontrib>Hoegerman, Stanton F</creatorcontrib><creatorcontrib>Osgood, Christopher J</creatorcontrib><creatorcontrib>Stacey, M.W</creatorcontrib><creatorcontrib>Mayer, Jacob</creatorcontrib><creatorcontrib>Oehninger, Sergio</creatorcontrib><creatorcontrib>Kearns, William G</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Fertility and sterility</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pfeffer, Jerome</au><au>Pang, Myung-Geol</au><au>Hoegerman, Stanton F</au><au>Osgood, Christopher J</au><au>Stacey, M.W</au><au>Mayer, Jacob</au><au>Oehninger, Sergio</au><au>Kearns, William G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Aneuploidy frequencies in semen fractions from ten oligoasthenoteratozoospermic patients donating sperm for intracytoplasmic sperm injection</atitle><jtitle>Fertility and sterility</jtitle><addtitle>Fertil Steril</addtitle><date>1999-09-01</date><risdate>1999</risdate><volume>72</volume><issue>3</issue><spage>472</spage><epage>478</epage><pages>472-478</pages><issn>0015-0282</issn><eissn>1556-5653</eissn><coden>FESTAS</coden><abstract>Objective: To determine aneuploidy frequencies in pellet and swim-up semen fractions from 10 infertile men with severe oligoasthenoteratozoospermia (OAT) who were donating sperm for intracytoplasmic sperm injection and to determine whether the swim-up isolation method would successfully separate aneuploid from haploid sperm.
Design: Prospective study.
Setting: Infertility clinic and molecular genetics laboratory.
Patient(s): Ten patients with severe OAT.
Intervention(s): Cytogenetic analyses by fluorescence in situ hybridization to determine aneuploidy frequencies for chromosomes 1, 13, 18, 21, X, and Y in sperm from swim-up and pellet fractions.
Main Outcome Measure(s): Gametic aneuploidy was scored in sperm fractions separated by the swim-up technique and clinical results after intracytoplasmic sperm injection were tabulated.
Result(s): In all cases, chromosome aneuploidy levels in patients were significantly greater than in controls. The type and percentage of aneuploid sperm for all patients with OAT found in both swim-up and pellet fractions were not different, with the exception of diploid sperm, which remained in the pellet fraction. After ET, 2 (20%) of 10 couples achieved successful pregnancies.
Conclusion(s): The data show significantly higher rates of diploidy, autosomal disomy and nullisomy, sex chromosome disomy and nullisomy, and total aneuploidy in sperm from all separated fractions obtained from all patients with OAT versus controls. This patient population with OAT may be at increased risk of producing aneuploid offspring.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>10519619</pmid><doi>10.1016/S0015-0282(99)00279-4</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Aneuploidy Biological and medical sciences Diploidy DNA - analysis Embryo Transfer Female Fertilization in Vitro - methods FISH Fluorescence in situ hybridization Gynecology. Andrology. Obstetrics Humans ICSI In Situ Hybridization, Fluorescence infertility intracytoplasmic sperm injection Male Male genital diseases Medical sciences Microinjections Non tumoral diseases OAT oligoasthenoteratozoospermia Oligospermia - genetics Oocytes - ultrastructure Pregnancy Prospective Studies Risk Factors Semen - chemistry Sperm Count Sperm Motility Spermatozoa - abnormalities swim-up separation Tissue Donors |
| title | Aneuploidy frequencies in semen fractions from ten oligoasthenoteratozoospermic patients donating sperm for intracytoplasmic sperm injection |
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