Isolation and sequence of the MIG1 homologue from the yeast Candida utilis

The Mig1p repressor from the food yeast Candida utilis has been isolated using a homologous PCR hybridization probe. This probe was amplified with two sets of degenerate primers designed on the basis of highly conserved motifs in the DNA‐binding region (zinc‐finger domain) from yeast Mig1p and fungi...

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Veröffentlicht in:	Yeast (Chichester, England) England), 2001-05, Vol.18 (7), p.597-603
Hauptverfasser:	Delfin, Julieta, Perdomo, Walmer, García, Bianca, Menendez, Javier
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Base Sequence Blotting, Southern Candida - chemistry Candida - genetics Candida utilis catabolite repression Cloning, Molecular DNA, Fungal - genetics DNA, Fungal - isolation & purification DNA-Binding Proteins - genetics DNA-Binding Proteins - isolation & purification MIG1 Molecular Sequence Data Phylogeny Polymerase Chain Reaction Repressor Proteins - genetics Repressor Proteins - isolation & purification Saccharomyces cerevisiae - chemistry Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae Proteins Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid yeast zinc‐finger protein
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