Effects of peritoneal macrophages from women with endometriosis on endometrial cellular proliferation in an in vitro coculture model
Objective: To study the effects of peritoneal macrophages on endometrial cellular proliferation in an in vitro coculture model and to compare the magnitude of these effects between macrophages from women with endometriosis and normal women. Design: Controlled study of peritoneal macrophage function....
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| Veröffentlicht in: | Fertility and sterility 1999-09, Vol.72 (3), p.533-538 |
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| container_title | Fertility and sterility |
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| creator | Loh, Foo-Hoe Bongso, Ariff Fong, Chui-Yee Koh, Dow-Rhoon Lee, Szu-Hee Zhao, Hui-Qin |
| description | Objective: To study the effects of peritoneal macrophages on endometrial cellular proliferation in an in vitro coculture model and to compare the magnitude of these effects between macrophages from women with endometriosis and normal women.
Design: Controlled study of peritoneal macrophage function.
Setting: University hospital.
Patient(s): Patients with a normal peritoneal cavity (n = 15) and with pelvic endometriosis (n = 20) undergoing laparoscopy.
Intervention(s): Peritoneal macrophages were cocultured with endometrial epithelial and stromal cells; endometrial cell cultures without macrophage coculture acted as controls.
Main Outcome Measure(s): Endometrial cellular proliferation measured by
3H-thymidine incorporation.
Result(s): Endometrial epithelial cells cocultured with peritoneal macrophages from women with endometriosis showed significantly increased proliferation compared with cocultures using macrophages from normal women when assessed at 24 hours (1.56 versus 1.03 times, respectively, over control) and at 72 hours (1.55 versus 1.10 times over control). Endometrial stromal cells cocultured with peritoneal macrophages from women with endometriosis similarly exhibited increased proliferation compared with cocultures using macrophages from normal women when assessed at 24 hours (1.65 versus 1.17 times over control) and at 72 hours (1.65 versus 1.21 times over control).
Conclusion(s): Peritoneal macrophages of patients with endometriosis stimulate cellular proliferation of endometrial epithelial and stromal cells in vitro. |
| doi_str_mv | 10.1016/S0015-0282(99)00292-7 |
| format | Article |
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Design: Controlled study of peritoneal macrophage function.
Setting: University hospital.
Patient(s): Patients with a normal peritoneal cavity (n = 15) and with pelvic endometriosis (n = 20) undergoing laparoscopy.
Intervention(s): Peritoneal macrophages were cocultured with endometrial epithelial and stromal cells; endometrial cell cultures without macrophage coculture acted as controls.
Main Outcome Measure(s): Endometrial cellular proliferation measured by
3H-thymidine incorporation.
Result(s): Endometrial epithelial cells cocultured with peritoneal macrophages from women with endometriosis showed significantly increased proliferation compared with cocultures using macrophages from normal women when assessed at 24 hours (1.56 versus 1.03 times, respectively, over control) and at 72 hours (1.55 versus 1.10 times over control). Endometrial stromal cells cocultured with peritoneal macrophages from women with endometriosis similarly exhibited increased proliferation compared with cocultures using macrophages from normal women when assessed at 24 hours (1.65 versus 1.17 times over control) and at 72 hours (1.65 versus 1.21 times over control).
Conclusion(s): Peritoneal macrophages of patients with endometriosis stimulate cellular proliferation of endometrial epithelial and stromal cells in vitro.</description><identifier>ISSN: 0015-0282</identifier><identifier>EISSN: 1556-5653</identifier><identifier>DOI: 10.1016/S0015-0282(99)00292-7</identifier><identifier>PMID: 10519630</identifier><identifier>CODEN: FESTAS</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Adult ; Ascitic Fluid - pathology ; Ascitic Fluid - physiopathology ; Biological and medical sciences ; Cell cycle, cell proliferation ; Cell Division ; Cell physiology ; coculture ; Coculture Techniques ; endometrial proliferation ; endometriosis ; Endometriosis - pathology ; Endometrium - pathology ; Epithelial Cells - pathology ; Female ; Fundamental and applied biological sciences. Psychology ; Humans ; Macrophages, Peritoneal - physiology ; Molecular and cellular biology ; Peritoneal macrophages ; Stromal Cells - pathology</subject><ispartof>Fertility and sterility, 1999-09, Vol.72 (3), p.533-538</ispartof><rights>1999 American Society for Reproductive Medicine</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c437t-39e920bce282e1080eff79d7d1bbddfc9f34d52f13f928136a0b280426d64e283</citedby><cites>FETCH-LOGICAL-c437t-39e920bce282e1080eff79d7d1bbddfc9f34d52f13f928136a0b280426d64e283</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0015028299002927$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1178376$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10519630$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Loh, Foo-Hoe</creatorcontrib><creatorcontrib>Bongso, Ariff</creatorcontrib><creatorcontrib>Fong, Chui-Yee</creatorcontrib><creatorcontrib>Koh, Dow-Rhoon</creatorcontrib><creatorcontrib>Lee, Szu-Hee</creatorcontrib><creatorcontrib>Zhao, Hui-Qin</creatorcontrib><title>Effects of peritoneal macrophages from women with endometriosis on endometrial cellular proliferation in an in vitro coculture model</title><title>Fertility and sterility</title><addtitle>Fertil Steril</addtitle><description>Objective: To study the effects of peritoneal macrophages on endometrial cellular proliferation in an in vitro coculture model and to compare the magnitude of these effects between macrophages from women with endometriosis and normal women.
Design: Controlled study of peritoneal macrophage function.
Setting: University hospital.
Patient(s): Patients with a normal peritoneal cavity (n = 15) and with pelvic endometriosis (n = 20) undergoing laparoscopy.
Intervention(s): Peritoneal macrophages were cocultured with endometrial epithelial and stromal cells; endometrial cell cultures without macrophage coculture acted as controls.
Main Outcome Measure(s): Endometrial cellular proliferation measured by
3H-thymidine incorporation.
Result(s): Endometrial epithelial cells cocultured with peritoneal macrophages from women with endometriosis showed significantly increased proliferation compared with cocultures using macrophages from normal women when assessed at 24 hours (1.56 versus 1.03 times, respectively, over control) and at 72 hours (1.55 versus 1.10 times over control). Endometrial stromal cells cocultured with peritoneal macrophages from women with endometriosis similarly exhibited increased proliferation compared with cocultures using macrophages from normal women when assessed at 24 hours (1.65 versus 1.17 times over control) and at 72 hours (1.65 versus 1.21 times over control).
Conclusion(s): Peritoneal macrophages of patients with endometriosis stimulate cellular proliferation of endometrial epithelial and stromal cells in vitro.</description><subject>Adult</subject><subject>Ascitic Fluid - pathology</subject><subject>Ascitic Fluid - physiopathology</subject><subject>Biological and medical sciences</subject><subject>Cell cycle, cell proliferation</subject><subject>Cell Division</subject><subject>Cell physiology</subject><subject>coculture</subject><subject>Coculture Techniques</subject><subject>endometrial proliferation</subject><subject>endometriosis</subject><subject>Endometriosis - pathology</subject><subject>Endometrium - pathology</subject><subject>Epithelial Cells - pathology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Macrophages, Peritoneal - physiology</subject><subject>Molecular and cellular biology</subject><subject>Peritoneal macrophages</subject><subject>Stromal Cells - pathology</subject><issn>0015-0282</issn><issn>1556-5653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1vFSEUhonR2Gv1J2hYGKOL0QPcGYaVMU39SJq4UNeEgYPFMMMVmDbu_eFyP2LduYFAnhdeHgh5yuA1Aza8-QLA-g74yF8q9QqAK97Je2TD-n7o-qEX98nmL3JGHpXyAwAGJvlDcsagZ2oQsCG_L71HWwtNnu4wh5oWNJHOxua0uzbfsVCf00xv04wLvQ31muLi2qLmkEpoueVuowUtxrhGk-kupxg8ZlNDQ8JCzWG8CTUnapNdY10z0jk5jI_JA29iwSen-Zx8e3_59eJjd_X5w6eLd1ed3QpZO6FQcZgsthchgxHQe6mcdGyanPNWebF1PfdMeMVHJgYDEx9hywc3bFtInJMXx3NbuZ8rlqrnUPaNzYJpLVrCyCQw0cD-CDYLpWT0epfDbPIvzUDv9euDfr13q5XSB_1attyz0wXrNKP7J3X03YDnJ8AUa6LPZrGh3HFMjkIODXt7xLDZuAmYdbEBF4su5PZb2qXwnyZ_AEiPpGQ</recordid><startdate>19990901</startdate><enddate>19990901</enddate><creator>Loh, Foo-Hoe</creator><creator>Bongso, Ariff</creator><creator>Fong, Chui-Yee</creator><creator>Koh, Dow-Rhoon</creator><creator>Lee, Szu-Hee</creator><creator>Zhao, Hui-Qin</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990901</creationdate><title>Effects of peritoneal macrophages from women with endometriosis on endometrial cellular proliferation in an in vitro coculture model</title><author>Loh, Foo-Hoe ; Bongso, Ariff ; Fong, Chui-Yee ; Koh, Dow-Rhoon ; Lee, Szu-Hee ; Zhao, Hui-Qin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c437t-39e920bce282e1080eff79d7d1bbddfc9f34d52f13f928136a0b280426d64e283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Adult</topic><topic>Ascitic Fluid - pathology</topic><topic>Ascitic Fluid - physiopathology</topic><topic>Biological and medical sciences</topic><topic>Cell cycle, cell proliferation</topic><topic>Cell Division</topic><topic>Cell physiology</topic><topic>coculture</topic><topic>Coculture Techniques</topic><topic>endometrial proliferation</topic><topic>endometriosis</topic><topic>Endometriosis - pathology</topic><topic>Endometrium - pathology</topic><topic>Epithelial Cells - pathology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Macrophages, Peritoneal - physiology</topic><topic>Molecular and cellular biology</topic><topic>Peritoneal macrophages</topic><topic>Stromal Cells - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Loh, Foo-Hoe</creatorcontrib><creatorcontrib>Bongso, Ariff</creatorcontrib><creatorcontrib>Fong, Chui-Yee</creatorcontrib><creatorcontrib>Koh, Dow-Rhoon</creatorcontrib><creatorcontrib>Lee, Szu-Hee</creatorcontrib><creatorcontrib>Zhao, Hui-Qin</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Fertility and sterility</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Loh, Foo-Hoe</au><au>Bongso, Ariff</au><au>Fong, Chui-Yee</au><au>Koh, Dow-Rhoon</au><au>Lee, Szu-Hee</au><au>Zhao, Hui-Qin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of peritoneal macrophages from women with endometriosis on endometrial cellular proliferation in an in vitro coculture model</atitle><jtitle>Fertility and sterility</jtitle><addtitle>Fertil Steril</addtitle><date>1999-09-01</date><risdate>1999</risdate><volume>72</volume><issue>3</issue><spage>533</spage><epage>538</epage><pages>533-538</pages><issn>0015-0282</issn><eissn>1556-5653</eissn><coden>FESTAS</coden><abstract>Objective: To study the effects of peritoneal macrophages on endometrial cellular proliferation in an in vitro coculture model and to compare the magnitude of these effects between macrophages from women with endometriosis and normal women.
Design: Controlled study of peritoneal macrophage function.
Setting: University hospital.
Patient(s): Patients with a normal peritoneal cavity (n = 15) and with pelvic endometriosis (n = 20) undergoing laparoscopy.
Intervention(s): Peritoneal macrophages were cocultured with endometrial epithelial and stromal cells; endometrial cell cultures without macrophage coculture acted as controls.
Main Outcome Measure(s): Endometrial cellular proliferation measured by
3H-thymidine incorporation.
Result(s): Endometrial epithelial cells cocultured with peritoneal macrophages from women with endometriosis showed significantly increased proliferation compared with cocultures using macrophages from normal women when assessed at 24 hours (1.56 versus 1.03 times, respectively, over control) and at 72 hours (1.55 versus 1.10 times over control). Endometrial stromal cells cocultured with peritoneal macrophages from women with endometriosis similarly exhibited increased proliferation compared with cocultures using macrophages from normal women when assessed at 24 hours (1.65 versus 1.17 times over control) and at 72 hours (1.65 versus 1.21 times over control).
Conclusion(s): Peritoneal macrophages of patients with endometriosis stimulate cellular proliferation of endometrial epithelial and stromal cells in vitro.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>10519630</pmid><doi>10.1016/S0015-0282(99)00292-7</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Fertility and sterility, 1999-09, Vol.72 (3), p.533-538 |
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| source | MEDLINE; Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Adult Ascitic Fluid - pathology Ascitic Fluid - physiopathology Biological and medical sciences Cell cycle, cell proliferation Cell Division Cell physiology coculture Coculture Techniques endometrial proliferation endometriosis Endometriosis - pathology Endometrium - pathology Epithelial Cells - pathology Female Fundamental and applied biological sciences. Psychology Humans Macrophages, Peritoneal - physiology Molecular and cellular biology Peritoneal macrophages Stromal Cells - pathology |
| title | Effects of peritoneal macrophages from women with endometriosis on endometrial cellular proliferation in an in vitro coculture model |
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