Construction of a framework map in Pinus contorta subsp. latifolia using random amplified polymorphic DNA markers

We report on the construction of the first random amplified polymorphic DNA (RAPD) framework map in Pinus contorta subsp. latifolia. Genomic DNA of haploid megagametophytes from 90 open-pollinated seeds originating from a single tree were amplified with 840 random decamer oligonucleotide primers by...

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Veröffentlicht in:	Genome 2001-04, Vol.44 (2), p.147-153
Hauptverfasser:	Li, C, Yeh, F.C
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Chromosome Mapping Chromosomes Deoxyribonucleic acid DNA DNA Primers - genetics DNA, Plant - genetics Flowers & plants Genetic Linkage Genetic Markers Genetics genome size Genome, Plant genomics linkage (genetics) Pine trees Pines Pinus contorta polymerase chain reaction R&D Random Amplified Polymorphic DNA Technique Research & development Seedlings seeds Trees - genetics
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