An immunosensor with potential for the detection of viral antigens in body fluids, based on surface second harmonic generation
Field methods of assessing the immune status of animals are required to optimise vaccination programmes to control bovine viral diarrhoea (BVD) virus. An optoelectronic immunosensor was evaluated for the detection of viral antigens in a crude cell lysate in a pilot study. Binding of (BVD) virus anti...
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| Veröffentlicht in: | Biosensors & bioelectronics 2001-05, Vol.16 (3), p.221-224 |
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| container_title | Biosensors & bioelectronics |
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| creator | Ditcham, W.G.F Al-Obaidi, A.H.R McStay, D Mottram, T.T Brownlie, J Thompson, I |
| description | Field methods of assessing the immune status of animals are required to optimise vaccination programmes to control bovine viral diarrhoea (BVD) virus. An optoelectronic immunosensor was evaluated for the detection of viral antigens in a crude cell lysate in a pilot study. Binding of (BVD) virus antigen by two monoclonal antibodies immobilised on two different media (ELISA plate wells, and glass coverslips) was detected and quantified using the laser induced surface second harmonic generation (SSHG) technique. The results for both assays were correlated with an enzyme-linked immunoassay (ELISA) used for the diagnosis of BVD virus infection in cattle (ELISA plate;
R
2=0.86, coverslips; Exp. 1;
R
2=0.75, Exp. 2;
R
2=0.67). The method will allow rapid detection of antigens in the body fluids of farm animals. |
| doi_str_mv | 10.1016/S0956-5663(00)00134-2 |
| format | Article |
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R
2=0.86, coverslips; Exp. 1;
R
2=0.75, Exp. 2;
R
2=0.67). The method will allow rapid detection of antigens in the body fluids of farm animals.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/S0956-5663(00)00134-2</identifier><identifier>PMID: 11340001</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Animals ; Antibodies, Monoclonal ; Antigens, Viral - analysis ; Antigens, Viral - blood ; Biological and medical sciences ; Biosensing Techniques - methods ; Biosensor ; Biosensors ; Biotechnology ; Body Fluids - virology ; Bovine ; Bovine viral diarrhea virus ; Bovine Virus Diarrhea-Mucosal Disease - diagnosis ; Bovine Virus Diarrhea-Mucosal Disease - immunology ; Bovine Virus Diarrhea-Mucosal Disease - prevention & control ; BVD ; Cattle ; Diarrhea Viruses, Bovine Viral - immunology ; Enzyme-Linked Immunosorbent Assay ; Fundamental and applied biological sciences. Psychology ; Immunosensor ; immunosensors ; Leukocytes - virology ; livestock ; Methods. Procedures. Technologies ; Optoelectronic ; Various methods and equipments</subject><ispartof>Biosensors & bioelectronics, 2001-05, Vol.16 (3), p.221-224</ispartof><rights>2001 Elsevier Science B.V.</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c468t-e7d8303651cad57929d63992b846a93c60c21a19d64c7c531d73f1ab0333113a3</citedby><cites>FETCH-LOGICAL-c468t-e7d8303651cad57929d63992b846a93c60c21a19d64c7c531d73f1ab0333113a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0956-5663(00)00134-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1086316$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11340001$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ditcham, W.G.F</creatorcontrib><creatorcontrib>Al-Obaidi, A.H.R</creatorcontrib><creatorcontrib>McStay, D</creatorcontrib><creatorcontrib>Mottram, T.T</creatorcontrib><creatorcontrib>Brownlie, J</creatorcontrib><creatorcontrib>Thompson, I</creatorcontrib><title>An immunosensor with potential for the detection of viral antigens in body fluids, based on surface second harmonic generation</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>Field methods of assessing the immune status of animals are required to optimise vaccination programmes to control bovine viral diarrhoea (BVD) virus. An optoelectronic immunosensor was evaluated for the detection of viral antigens in a crude cell lysate in a pilot study. Binding of (BVD) virus antigen by two monoclonal antibodies immobilised on two different media (ELISA plate wells, and glass coverslips) was detected and quantified using the laser induced surface second harmonic generation (SSHG) technique. The results for both assays were correlated with an enzyme-linked immunoassay (ELISA) used for the diagnosis of BVD virus infection in cattle (ELISA plate;
R
2=0.86, coverslips; Exp. 1;
R
2=0.75, Exp. 2;
R
2=0.67). The method will allow rapid detection of antigens in the body fluids of farm animals.</description><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Antigens, Viral - analysis</subject><subject>Antigens, Viral - blood</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques - methods</subject><subject>Biosensor</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Body Fluids - virology</subject><subject>Bovine</subject><subject>Bovine viral diarrhea virus</subject><subject>Bovine Virus Diarrhea-Mucosal Disease - diagnosis</subject><subject>Bovine Virus Diarrhea-Mucosal Disease - immunology</subject><subject>Bovine Virus Diarrhea-Mucosal Disease - prevention & control</subject><subject>BVD</subject><subject>Cattle</subject><subject>Diarrhea Viruses, Bovine Viral - immunology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunosensor</subject><subject>immunosensors</subject><subject>Leukocytes - virology</subject><subject>livestock</subject><subject>Methods. Procedures. Technologies</subject><subject>Optoelectronic</subject><subject>Various methods and equipments</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxS0EotvCRwD5gBBIBGbixE5OVVWVP1IlDsDZcuwJa5TYi50U9cJnx9tdAbeeLM_83rNnHmPPEN4ioHz3BfpWVq2U4hXAawAUTVU_YBvslKiaWrQP2eYvcsJOc_4BAAp7eMxOsNDlhhv2-yJwP89riJlCjon_8suW7-JCYfFm4mMpLVvijhayi4-Bx5Hf-FRaphDfi4j7wIfobvk4rd7lN3wwmRwvaF7TaCzxTDYGx7cmzTF4y4uKktm7PWGPRjNleno8z9i391dfLz9W158_fLq8uK5sI7ulIuU6AUK2aI1rVV_3Toq-r4eukaYXVoKt0WCpNlbZVqBTYkQzgBCizGrEGXt58N2l-HOlvOjZZ0vTZALFNWsFHbaA7b0gdihBKVXA9gDaFHNONOpd8rNJtxpB7xPSdwnp_fo1gL5LSNdF9_z4wDrM5P6pjpEU4MURMNmaaUwmWJ__c--kQFmw8wNGZW03npLO1lOw5HwqSWkX_T0_-QOjeK1d</recordid><startdate>20010501</startdate><enddate>20010501</enddate><creator>Ditcham, W.G.F</creator><creator>Al-Obaidi, A.H.R</creator><creator>McStay, D</creator><creator>Mottram, T.T</creator><creator>Brownlie, J</creator><creator>Thompson, I</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20010501</creationdate><title>An immunosensor with potential for the detection of viral antigens in body fluids, based on surface second harmonic generation</title><author>Ditcham, W.G.F ; Al-Obaidi, A.H.R ; McStay, D ; Mottram, T.T ; Brownlie, J ; Thompson, I</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c468t-e7d8303651cad57929d63992b846a93c60c21a19d64c7c531d73f1ab0333113a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Antigens, Viral - analysis</topic><topic>Antigens, Viral - blood</topic><topic>Biological and medical sciences</topic><topic>Biosensing Techniques - methods</topic><topic>Biosensor</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Body Fluids - virology</topic><topic>Bovine</topic><topic>Bovine viral diarrhea virus</topic><topic>Bovine Virus Diarrhea-Mucosal Disease - diagnosis</topic><topic>Bovine Virus Diarrhea-Mucosal Disease - immunology</topic><topic>Bovine Virus Diarrhea-Mucosal Disease - prevention & control</topic><topic>BVD</topic><topic>Cattle</topic><topic>Diarrhea Viruses, Bovine Viral - immunology</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunosensor</topic><topic>immunosensors</topic><topic>Leukocytes - virology</topic><topic>livestock</topic><topic>Methods. Procedures. Technologies</topic><topic>Optoelectronic</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ditcham, W.G.F</creatorcontrib><creatorcontrib>Al-Obaidi, A.H.R</creatorcontrib><creatorcontrib>McStay, D</creatorcontrib><creatorcontrib>Mottram, T.T</creatorcontrib><creatorcontrib>Brownlie, J</creatorcontrib><creatorcontrib>Thompson, I</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ditcham, W.G.F</au><au>Al-Obaidi, A.H.R</au><au>McStay, D</au><au>Mottram, T.T</au><au>Brownlie, J</au><au>Thompson, I</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An immunosensor with potential for the detection of viral antigens in body fluids, based on surface second harmonic generation</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2001-05-01</date><risdate>2001</risdate><volume>16</volume><issue>3</issue><spage>221</spage><epage>224</epage><pages>221-224</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>Field methods of assessing the immune status of animals are required to optimise vaccination programmes to control bovine viral diarrhoea (BVD) virus. An optoelectronic immunosensor was evaluated for the detection of viral antigens in a crude cell lysate in a pilot study. Binding of (BVD) virus antigen by two monoclonal antibodies immobilised on two different media (ELISA plate wells, and glass coverslips) was detected and quantified using the laser induced surface second harmonic generation (SSHG) technique. The results for both assays were correlated with an enzyme-linked immunoassay (ELISA) used for the diagnosis of BVD virus infection in cattle (ELISA plate;
R
2=0.86, coverslips; Exp. 1;
R
2=0.75, Exp. 2;
R
2=0.67). The method will allow rapid detection of antigens in the body fluids of farm animals.</abstract><cop>Lausanne</cop><pub>Elsevier B.V</pub><pmid>11340001</pmid><doi>10.1016/S0956-5663(00)00134-2</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Antibodies, Monoclonal Antigens, Viral - analysis Antigens, Viral - blood Biological and medical sciences Biosensing Techniques - methods Biosensor Biosensors Biotechnology Body Fluids - virology Bovine Bovine viral diarrhea virus Bovine Virus Diarrhea-Mucosal Disease - diagnosis Bovine Virus Diarrhea-Mucosal Disease - immunology Bovine Virus Diarrhea-Mucosal Disease - prevention & control BVD Cattle Diarrhea Viruses, Bovine Viral - immunology Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology Immunosensor immunosensors Leukocytes - virology livestock Methods. Procedures. Technologies Optoelectronic Various methods and equipments |
| title | An immunosensor with potential for the detection of viral antigens in body fluids, based on surface second harmonic generation |
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