Vaccination of sheep against Fasciola hepatica with homologous fatty acid binding proteins
The current study was designed to test the immunoprophylactic properties of native (nFh12) and recombinant (rFh15) antigens from Fasciola hepatica in sheep subsequently infected with the fluke. Thirty lambs were divided into six groups according to various patterns of immunisation and times of infec...
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| Veröffentlicht in: | Veterinary parasitology 2001-05, Vol.97 (1), p.35-46 |
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| creator | Ramajo, V Oleaga, A Casanueva, P Hillyer, G.V Muro, A |
| description | The current study was designed to test the immunoprophylactic properties of native (nFh12) and recombinant (rFh15) antigens from
Fasciola hepatica in sheep subsequently infected with the fluke. Thirty lambs were divided into six groups according to various patterns of immunisation and times of infection and necropsy. The antigens were emulsified in Freund’s adjuvant. Levels of specific anti-nFh12 and anti-rFh15 antibodies rose rapidly by 2 weeks after the first immunisation and were always significantly higher in immunised-infected sheep than in control-infected sheep. On completion of the trial there was no difference in fluke burden between groups vaccinated with either of the antigens and non-immunised controls. However, worm size and faecal egg counts were significantly diminished in the sheep vaccinated with either of the antigens, suggesting an anti-fecundity effect. This is the first report of experimental vaccination of sheep against
F. hepatica with purified native and recombinant antigens related to fatty acid binding proteins. |
| doi_str_mv | 10.1016/S0304-4017(01)00388-0 |
| format | Article |
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Fasciola hepatica in sheep subsequently infected with the fluke. Thirty lambs were divided into six groups according to various patterns of immunisation and times of infection and necropsy. The antigens were emulsified in Freund’s adjuvant. Levels of specific anti-nFh12 and anti-rFh15 antibodies rose rapidly by 2 weeks after the first immunisation and were always significantly higher in immunised-infected sheep than in control-infected sheep. On completion of the trial there was no difference in fluke burden between groups vaccinated with either of the antigens and non-immunised controls. However, worm size and faecal egg counts were significantly diminished in the sheep vaccinated with either of the antigens, suggesting an anti-fecundity effect. This is the first report of experimental vaccination of sheep against
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F. hepatica with purified native and recombinant antigens related to fatty acid binding proteins.</description><subject>Animals</subject><subject>Carrier Proteins - immunology</subject><subject>FABP</subject><subject>Fasciola hepatica</subject><subject>Fascioliasis - prevention & control</subject><subject>Fascioliasis - veterinary</subject><subject>Fatty Acid-Binding Proteins</subject><subject>Feces - parasitology</subject><subject>Female</subject><subject>Fertility - drug effects</subject><subject>Freund's Adjuvant - immunology</subject><subject>Lymnaea - parasitology</subject><subject>Neoplasm Proteins</subject><subject>Parasite Egg Count - veterinary</subject><subject>Sheep</subject><subject>Sheep Diseases - prevention & control</subject><subject>Sheep-trematoda</subject><subject>Vaccination - veterinary</subject><subject>Vaccine</subject><issn>0304-4017</issn><issn>1873-2550</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v1DAQhi0EotvCTwD5hNpD6IydxMkJoapfUqUeWjhwsSbOZNcoG29jL6j_vm53VY6c5vK88848QnxC-IqA9ekdaCiLEtAcA54A6KYp4I1YYGN0oaoK3orFK3IgDmP8DQAl1Oa9OEDU2qCqFuLXT3LOT5R8mGQYZFwxbyQtyU8xyQuKzoeR5Io3GXEk__q0kquwDmNYhm2UA6X0KMn5XnZ-6v20lJs5JM7xD-LdQGPkj_t5JH5cnN-fXRU3t5fXZ99vCqdrTEXbcNchAZNSqlQ8GNU2_aArosHlk7E1JTG2bY_KVLVuVW1yRHdmUDVho4_El93eXPyw5Zjs2kfH40gT5xOtgQZzE2aw2oFuDjHOPNjN7Nc0P1oE-yzVvki1z8YsoH2RaiHnPu8Ltt2a-3-pvcUMfNsBnN_843m2WRtPjns_s0u2D_4_FU-ZMIa6</recordid><startdate>20010509</startdate><enddate>20010509</enddate><creator>Ramajo, V</creator><creator>Oleaga, A</creator><creator>Casanueva, P</creator><creator>Hillyer, G.V</creator><creator>Muro, A</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010509</creationdate><title>Vaccination of sheep against Fasciola hepatica with homologous fatty acid binding proteins</title><author>Ramajo, V ; Oleaga, A ; Casanueva, P ; Hillyer, G.V ; Muro, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c361t-98ebb1a0ea22242ef7298df35aafc0001974ae199d12756392678eb3b7f26a183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Carrier Proteins - immunology</topic><topic>FABP</topic><topic>Fasciola hepatica</topic><topic>Fascioliasis - prevention & control</topic><topic>Fascioliasis - veterinary</topic><topic>Fatty Acid-Binding Proteins</topic><topic>Feces - parasitology</topic><topic>Female</topic><topic>Fertility - drug effects</topic><topic>Freund's Adjuvant - immunology</topic><topic>Lymnaea - parasitology</topic><topic>Neoplasm Proteins</topic><topic>Parasite Egg Count - veterinary</topic><topic>Sheep</topic><topic>Sheep Diseases - prevention & control</topic><topic>Sheep-trematoda</topic><topic>Vaccination - veterinary</topic><topic>Vaccine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ramajo, V</creatorcontrib><creatorcontrib>Oleaga, A</creatorcontrib><creatorcontrib>Casanueva, P</creatorcontrib><creatorcontrib>Hillyer, G.V</creatorcontrib><creatorcontrib>Muro, A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ramajo, V</au><au>Oleaga, A</au><au>Casanueva, P</au><au>Hillyer, G.V</au><au>Muro, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Vaccination of sheep against Fasciola hepatica with homologous fatty acid binding proteins</atitle><jtitle>Veterinary parasitology</jtitle><addtitle>Vet Parasitol</addtitle><date>2001-05-09</date><risdate>2001</risdate><volume>97</volume><issue>1</issue><spage>35</spage><epage>46</epage><pages>35-46</pages><issn>0304-4017</issn><eissn>1873-2550</eissn><abstract>The current study was designed to test the immunoprophylactic properties of native (nFh12) and recombinant (rFh15) antigens from
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| identifier | ISSN: 0304-4017 |
| ispartof | Veterinary parasitology, 2001-05, Vol.97 (1), p.35-46 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Carrier Proteins - immunology FABP Fasciola hepatica Fascioliasis - prevention & control Fascioliasis - veterinary Fatty Acid-Binding Proteins Feces - parasitology Female Fertility - drug effects Freund's Adjuvant - immunology Lymnaea - parasitology Neoplasm Proteins Parasite Egg Count - veterinary Sheep Sheep Diseases - prevention & control Sheep-trematoda Vaccination - veterinary Vaccine |
| title | Vaccination of sheep against Fasciola hepatica with homologous fatty acid binding proteins |
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