Near-infrared and nuclear magnetic resonance spectroscopic assessment of tissue energetics in an isolated, perfused canine hind limb model of dysoxia
This controlled laboratory study examined the efficacy of near-infrared spectroscopy (NIRS) and 31P-nuclear magnetic resonance (NMR) spectroscopy in measuring regional tissue oxygenation in a isolated, perfused hind limb model of tissue dysoxia. Isolated hind limb perfusion was carried out in 20 mon...
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| Veröffentlicht in: | Shock (Augusta, Ga.) Ga.), 2001-05, Vol.15 (5), p.392-397 |
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| creator | BEILMAN, Greg J MYERS, Dean CERRA, Frank B LAZARON, Victor DAHMS, Rachel A CONROY, Mark J HAMMER, Bruce E |
| description | This controlled laboratory study examined the efficacy of near-infrared spectroscopy (NIRS) and 31P-nuclear magnetic resonance (NMR) spectroscopy in measuring regional tissue oxygenation in a isolated, perfused hind limb model of tissue dysoxia. Isolated hind limb perfusion was carried out in 20 mongrel dogs and oxygen delivery was varied by manipulating either hemoglobin concentration, oxygen saturation, or flow. Hind limbs from anesthetized mongrel dogs (n = 20) were separated and isolated perfusion performed. NIRS probes for recording relative O2 saturation of tissue hemoglobin (HbO2) and cytochrome a,a3 and NMR probes for measuring 31P-high energy phosphates were placed over the limb. Measurements of physiologic parameters, blood gases, lactate, NIRS values for HbO2 and cytochrome a,a3 redox state, and 31P-phosphate levels were recorded at set intervals throughout the experiment. Measures of tissue oxygen consumption (VO2) correlated with tissue oxygenation as measured by HbO2 and cytochrome a,a3 redox state (NIRS), as well as by 31P-high energy phosphate levels (NMR) throughout the experiment. Delivery-dependent tissue oxygenation was detected at a higher DO2 by NIRS than by VO2 or NMR. Tissue oxygenation as measured by NIRS and NMR shows excellent correlation with oxygen delivery in an isolated, perfused model of shock. NIRS may allow early detection of tissue dysoxia using rapid non-invasive techniques. |
| doi_str_mv | 10.1097/00024382-200115050-00010 |
| format | Article |
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Isolated hind limb perfusion was carried out in 20 mongrel dogs and oxygen delivery was varied by manipulating either hemoglobin concentration, oxygen saturation, or flow. Hind limbs from anesthetized mongrel dogs (n = 20) were separated and isolated perfusion performed. NIRS probes for recording relative O2 saturation of tissue hemoglobin (HbO2) and cytochrome a,a3 and NMR probes for measuring 31P-high energy phosphates were placed over the limb. Measurements of physiologic parameters, blood gases, lactate, NIRS values for HbO2 and cytochrome a,a3 redox state, and 31P-phosphate levels were recorded at set intervals throughout the experiment. Measures of tissue oxygen consumption (VO2) correlated with tissue oxygenation as measured by HbO2 and cytochrome a,a3 redox state (NIRS), as well as by 31P-high energy phosphate levels (NMR) throughout the experiment. Delivery-dependent tissue oxygenation was detected at a higher DO2 by NIRS than by VO2 or NMR. Tissue oxygenation as measured by NIRS and NMR shows excellent correlation with oxygen delivery in an isolated, perfused model of shock. NIRS may allow early detection of tissue dysoxia using rapid non-invasive techniques.</description><identifier>ISSN: 1073-2322</identifier><identifier>EISSN: 1540-0514</identifier><identifier>DOI: 10.1097/00024382-200115050-00010</identifier><identifier>PMID: 11336200</identifier><language>eng</language><publisher>Augusta, GA: BioMedical Press</publisher><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ; Animals ; Biological and medical sciences ; Dogs ; Emergency and intensive cardiocirculatory care. Cardiogenic shock. Coronary intensive care ; Emergency and intensive care: techniques, logistics ; Extremities - blood supply ; Extremities - physiology ; Intensive care medicine ; Magnetic Resonance Spectroscopy ; Medical sciences ; Monitoring ; Oxygen - metabolism ; Perfusion ; Spectroscopy, Near-Infrared</subject><ispartof>Shock (Augusta, Ga.), 2001-05, Vol.15 (5), p.392-397</ispartof><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-ec7d3c551c55b8a29ac5ab9ec86c3fe4e65ef405d3274f72383ce4f0c7de71773</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=973108$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11336200$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>BEILMAN, Greg J</creatorcontrib><creatorcontrib>MYERS, Dean</creatorcontrib><creatorcontrib>CERRA, Frank B</creatorcontrib><creatorcontrib>LAZARON, Victor</creatorcontrib><creatorcontrib>DAHMS, Rachel A</creatorcontrib><creatorcontrib>CONROY, Mark J</creatorcontrib><creatorcontrib>HAMMER, Bruce E</creatorcontrib><title>Near-infrared and nuclear magnetic resonance spectroscopic assessment of tissue energetics in an isolated, perfused canine hind limb model of dysoxia</title><title>Shock (Augusta, Ga.)</title><addtitle>Shock</addtitle><description>This controlled laboratory study examined the efficacy of near-infrared spectroscopy (NIRS) and 31P-nuclear magnetic resonance (NMR) spectroscopy in measuring regional tissue oxygenation in a isolated, perfused hind limb model of tissue dysoxia. Isolated hind limb perfusion was carried out in 20 mongrel dogs and oxygen delivery was varied by manipulating either hemoglobin concentration, oxygen saturation, or flow. Hind limbs from anesthetized mongrel dogs (n = 20) were separated and isolated perfusion performed. NIRS probes for recording relative O2 saturation of tissue hemoglobin (HbO2) and cytochrome a,a3 and NMR probes for measuring 31P-high energy phosphates were placed over the limb. Measurements of physiologic parameters, blood gases, lactate, NIRS values for HbO2 and cytochrome a,a3 redox state, and 31P-phosphate levels were recorded at set intervals throughout the experiment. Measures of tissue oxygen consumption (VO2) correlated with tissue oxygenation as measured by HbO2 and cytochrome a,a3 redox state (NIRS), as well as by 31P-high energy phosphate levels (NMR) throughout the experiment. Delivery-dependent tissue oxygenation was detected at a higher DO2 by NIRS than by VO2 or NMR. Tissue oxygenation as measured by NIRS and NMR shows excellent correlation with oxygen delivery in an isolated, perfused model of shock. NIRS may allow early detection of tissue dysoxia using rapid non-invasive techniques.</description><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Dogs</subject><subject>Emergency and intensive cardiocirculatory care. Cardiogenic shock. Coronary intensive care</subject><subject>Emergency and intensive care: techniques, logistics</subject><subject>Extremities - blood supply</subject><subject>Extremities - physiology</subject><subject>Intensive care medicine</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Medical sciences</subject><subject>Monitoring</subject><subject>Oxygen - metabolism</subject><subject>Perfusion</subject><subject>Spectroscopy, Near-Infrared</subject><issn>1073-2322</issn><issn>1540-0514</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkc1u1TAQhS1ERUvhFZAlJFYExnZ8nSxRVX6kCjbtOvKdjItRYgdPItEH4X3r217KwrJ9dL45so8QUsEHBb37CAC6NZ1uNIBSFiw0VVLwTJwp29aLVe3zegZnGm20PhUvmX89QL17IU6VMmZX2TPx9zv50sQUii80Sp9GmTacqihnf5tojSgLcU4-IUleCNeSGfNSdc9MzDOlVeYg18i8kaRE5faAsYypzpOR8-RXGt_LhUrYuKagTzGR_Blr2hTnvZzzSNNhyHjH-U_0r8RJ8BPT6-N-Lm4-X15ffG2ufnz5dvHpqkHT9WtD6EaD1qq69p3XvUfr9z1ht0MTqKWdpdCCHY12bXDadAapDVAxcso5cy7ePc5dSv69Ea_DHBlpmnyivPHgoDv8FFRj92jE-nouFIalxNmXu0HBcKhk-FfJ8FTJ8FBJRd8cM7b9TON_8NhBNbw9Gjyjn2oTCSM_-XpnFHTmHvKRltA</recordid><startdate>20010501</startdate><enddate>20010501</enddate><creator>BEILMAN, Greg J</creator><creator>MYERS, Dean</creator><creator>CERRA, Frank B</creator><creator>LAZARON, Victor</creator><creator>DAHMS, Rachel A</creator><creator>CONROY, Mark J</creator><creator>HAMMER, Bruce E</creator><general>BioMedical Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010501</creationdate><title>Near-infrared and nuclear magnetic resonance spectroscopic assessment of tissue energetics in an isolated, perfused canine hind limb model of dysoxia</title><author>BEILMAN, Greg J ; MYERS, Dean ; CERRA, Frank B ; LAZARON, Victor ; DAHMS, Rachel A ; CONROY, Mark J ; HAMMER, Bruce E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-ec7d3c551c55b8a29ac5ab9ec86c3fe4e65ef405d3274f72383ce4f0c7de71773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Dogs</topic><topic>Emergency and intensive cardiocirculatory care. Cardiogenic shock. Coronary intensive care</topic><topic>Emergency and intensive care: techniques, logistics</topic><topic>Extremities - blood supply</topic><topic>Extremities - physiology</topic><topic>Intensive care medicine</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Medical sciences</topic><topic>Monitoring</topic><topic>Oxygen - metabolism</topic><topic>Perfusion</topic><topic>Spectroscopy, Near-Infrared</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>BEILMAN, Greg J</creatorcontrib><creatorcontrib>MYERS, Dean</creatorcontrib><creatorcontrib>CERRA, Frank B</creatorcontrib><creatorcontrib>LAZARON, Victor</creatorcontrib><creatorcontrib>DAHMS, Rachel A</creatorcontrib><creatorcontrib>CONROY, Mark J</creatorcontrib><creatorcontrib>HAMMER, Bruce E</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Shock (Augusta, Ga.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>BEILMAN, Greg J</au><au>MYERS, Dean</au><au>CERRA, Frank B</au><au>LAZARON, Victor</au><au>DAHMS, Rachel A</au><au>CONROY, Mark J</au><au>HAMMER, Bruce E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Near-infrared and nuclear magnetic resonance spectroscopic assessment of tissue energetics in an isolated, perfused canine hind limb model of dysoxia</atitle><jtitle>Shock (Augusta, Ga.)</jtitle><addtitle>Shock</addtitle><date>2001-05-01</date><risdate>2001</risdate><volume>15</volume><issue>5</issue><spage>392</spage><epage>397</epage><pages>392-397</pages><issn>1073-2322</issn><eissn>1540-0514</eissn><abstract>This controlled laboratory study examined the efficacy of near-infrared spectroscopy (NIRS) and 31P-nuclear magnetic resonance (NMR) spectroscopy in measuring regional tissue oxygenation in a isolated, perfused hind limb model of tissue dysoxia. Isolated hind limb perfusion was carried out in 20 mongrel dogs and oxygen delivery was varied by manipulating either hemoglobin concentration, oxygen saturation, or flow. Hind limbs from anesthetized mongrel dogs (n = 20) were separated and isolated perfusion performed. NIRS probes for recording relative O2 saturation of tissue hemoglobin (HbO2) and cytochrome a,a3 and NMR probes for measuring 31P-high energy phosphates were placed over the limb. Measurements of physiologic parameters, blood gases, lactate, NIRS values for HbO2 and cytochrome a,a3 redox state, and 31P-phosphate levels were recorded at set intervals throughout the experiment. Measures of tissue oxygen consumption (VO2) correlated with tissue oxygenation as measured by HbO2 and cytochrome a,a3 redox state (NIRS), as well as by 31P-high energy phosphate levels (NMR) throughout the experiment. Delivery-dependent tissue oxygenation was detected at a higher DO2 by NIRS than by VO2 or NMR. Tissue oxygenation as measured by NIRS and NMR shows excellent correlation with oxygen delivery in an isolated, perfused model of shock. NIRS may allow early detection of tissue dysoxia using rapid non-invasive techniques.</abstract><cop>Augusta, GA</cop><pub>BioMedical Press</pub><pmid>11336200</pmid><doi>10.1097/00024382-200115050-00010</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Animals Biological and medical sciences Dogs Emergency and intensive cardiocirculatory care. Cardiogenic shock. Coronary intensive care Emergency and intensive care: techniques, logistics Extremities - blood supply Extremities - physiology Intensive care medicine Magnetic Resonance Spectroscopy Medical sciences Monitoring Oxygen - metabolism Perfusion Spectroscopy, Near-Infrared |
| title | Near-infrared and nuclear magnetic resonance spectroscopic assessment of tissue energetics in an isolated, perfused canine hind limb model of dysoxia |
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