Determination of Valproic Acid in Human Serum by High-Performance Liquid Chromatography with Fluorescence Detection
A simple and highly sensitive high-performance liquid chromatographic method is described for the determination of valproic acid in human serum. The method is based on the direct derivatization of serum sample with 6, 7-methylenedioxy-1-methyl-2-oxo-1, 2-dihydroquinoxaline-3-ylpropionohydrazide. The...
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| Veröffentlicht in: | Biological & pharmaceutical bulletin 1999/09/15, Vol.22(9), pp.975-977 |
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| container_title | Biological & pharmaceutical bulletin |
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| creator | HARA, Shuuji KAMURA, Motoki INOUE, Kazuo FUKUZAWA, Misa ONO, Nobufumi KURODA, Takeshi |
| description | A simple and highly sensitive high-performance liquid chromatographic method is described for the determination of valproic acid in human serum. The method is based on the direct derivatization of serum sample with 6, 7-methylenedioxy-1-methyl-2-oxo-1, 2-dihydroquinoxaline-3-ylpropionohydrazide. The derivatization reaction proceeds in aqueous solution in the presence of pyridine and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide at 37°C. The resulting derivatives were separated on a reversed-phase column (YMC Pack ODS-A) with isocratic elution and were fluorimetrically detected at 440 nm with excitation at 365 nm. The detection limit (signal-to-noise ratio=3) for valproic acid added to serum sample was 0.1 μg (700 fmol)/ml serum sample (2.3 fmol on column). The method was applied to determine the unbound- and total-valproic acid levels in the serum obtained from three healthy volunteers after oral administration of the drug (600 mg). |
| doi_str_mv | 10.1248/bpb.22.975 |
| format | Article |
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The method is based on the direct derivatization of serum sample with 6, 7-methylenedioxy-1-methyl-2-oxo-1, 2-dihydroquinoxaline-3-ylpropionohydrazide. The derivatization reaction proceeds in aqueous solution in the presence of pyridine and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide at 37°C. The resulting derivatives were separated on a reversed-phase column (YMC Pack ODS-A) with isocratic elution and were fluorimetrically detected at 440 nm with excitation at 365 nm. The detection limit (signal-to-noise ratio=3) for valproic acid added to serum sample was 0.1 μg (700 fmol)/ml serum sample (2.3 fmol on column). The method was applied to determine the unbound- and total-valproic acid levels in the serum obtained from three healthy volunteers after oral administration of the drug (600 mg).</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.22.975</identifier><identifier>PMID: 10513624</identifier><language>eng</language><publisher>Tokyo: The Pharmaceutical Society of Japan</publisher><subject>6, 7-methylenedioxy-1-methyl-2-oxo-1, 2-dihydroquinoxaline-3-ylpropionohydrazide ; Adult ; Analysis ; Anticonvulsants - blood ; Biological and medical sciences ; Chromatography, High Pressure Liquid - methods ; fluorescence detection ; General pharmacology ; HPLC ; Humans ; Medical sciences ; Pharmacology. Drug treatments ; Spectrometry, Fluorescence ; unbound ; valproic acid ; Valproic Acid - blood</subject><ispartof>Biological and Pharmaceutical Bulletin, 1999/09/15, Vol.22(9), pp.975-977</ispartof><rights>The Pharmaceutical Society of Japan</rights><rights>1999 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 1999</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c502t-b21b2ce8aeca320b56957e07c9edb01a07e17c54b76a92a2b84c402a9ca95d93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,1884,27926,27927</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1993845$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10513624$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HARA, Shuuji</creatorcontrib><creatorcontrib>KAMURA, Motoki</creatorcontrib><creatorcontrib>INOUE, Kazuo</creatorcontrib><creatorcontrib>FUKUZAWA, Misa</creatorcontrib><creatorcontrib>ONO, Nobufumi</creatorcontrib><creatorcontrib>KURODA, Takeshi</creatorcontrib><title>Determination of Valproic Acid in Human Serum by High-Performance Liquid Chromatography with Fluorescence Detection</title><title>Biological & pharmaceutical bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>A simple and highly sensitive high-performance liquid chromatographic method is described for the determination of valproic acid in human serum. The method is based on the direct derivatization of serum sample with 6, 7-methylenedioxy-1-methyl-2-oxo-1, 2-dihydroquinoxaline-3-ylpropionohydrazide. The derivatization reaction proceeds in aqueous solution in the presence of pyridine and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide at 37°C. The resulting derivatives were separated on a reversed-phase column (YMC Pack ODS-A) with isocratic elution and were fluorimetrically detected at 440 nm with excitation at 365 nm. The detection limit (signal-to-noise ratio=3) for valproic acid added to serum sample was 0.1 μg (700 fmol)/ml serum sample (2.3 fmol on column). The method was applied to determine the unbound- and total-valproic acid levels in the serum obtained from three healthy volunteers after oral administration of the drug (600 mg).</description><subject>6, 7-methylenedioxy-1-methyl-2-oxo-1, 2-dihydroquinoxaline-3-ylpropionohydrazide</subject><subject>Adult</subject><subject>Analysis</subject><subject>Anticonvulsants - blood</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>fluorescence detection</subject><subject>General pharmacology</subject><subject>HPLC</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Pharmacology. 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Drug treatments</topic><topic>Spectrometry, Fluorescence</topic><topic>unbound</topic><topic>valproic acid</topic><topic>Valproic Acid - blood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HARA, Shuuji</creatorcontrib><creatorcontrib>KAMURA, Motoki</creatorcontrib><creatorcontrib>INOUE, Kazuo</creatorcontrib><creatorcontrib>FUKUZAWA, Misa</creatorcontrib><creatorcontrib>ONO, Nobufumi</creatorcontrib><creatorcontrib>KURODA, Takeshi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biological & pharmaceutical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HARA, Shuuji</au><au>KAMURA, Motoki</au><au>INOUE, Kazuo</au><au>FUKUZAWA, Misa</au><au>ONO, Nobufumi</au><au>KURODA, Takeshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of Valproic Acid in Human Serum by High-Performance Liquid Chromatography with Fluorescence Detection</atitle><jtitle>Biological & pharmaceutical bulletin</jtitle><addtitle>Biol Pharm Bull</addtitle><date>1999-09-01</date><risdate>1999</risdate><volume>22</volume><issue>9</issue><spage>975</spage><epage>977</epage><pages>975-977</pages><issn>0918-6158</issn><eissn>1347-5215</eissn><abstract>A simple and highly sensitive high-performance liquid chromatographic method is described for the determination of valproic acid in human serum. The method is based on the direct derivatization of serum sample with 6, 7-methylenedioxy-1-methyl-2-oxo-1, 2-dihydroquinoxaline-3-ylpropionohydrazide. The derivatization reaction proceeds in aqueous solution in the presence of pyridine and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide at 37°C. The resulting derivatives were separated on a reversed-phase column (YMC Pack ODS-A) with isocratic elution and were fluorimetrically detected at 440 nm with excitation at 365 nm. The detection limit (signal-to-noise ratio=3) for valproic acid added to serum sample was 0.1 μg (700 fmol)/ml serum sample (2.3 fmol on column). The method was applied to determine the unbound- and total-valproic acid levels in the serum obtained from three healthy volunteers after oral administration of the drug (600 mg).</abstract><cop>Tokyo</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>10513624</pmid><doi>10.1248/bpb.22.975</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Biological and Pharmaceutical Bulletin, 1999/09/15, Vol.22(9), pp.975-977 |
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| source | J-STAGE Free; MEDLINE; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | 6, 7-methylenedioxy-1-methyl-2-oxo-1, 2-dihydroquinoxaline-3-ylpropionohydrazide Adult Analysis Anticonvulsants - blood Biological and medical sciences Chromatography, High Pressure Liquid - methods fluorescence detection General pharmacology HPLC Humans Medical sciences Pharmacology. Drug treatments Spectrometry, Fluorescence unbound valproic acid Valproic Acid - blood |
| title | Determination of Valproic Acid in Human Serum by High-Performance Liquid Chromatography with Fluorescence Detection |
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