Characterization and purification of porcine small intestinal mucus receptor for Escherichia coli K88ac fimbrial adhesin

Abstract The objectives of this study were to investigate the nature of, and to purify K88ac fimbrial adhesin-specific receptors in the mucus from the small intestine of piglet. Adhesion was studied by incubating 3H-labeled Escherichia coli with mucus that were treated with or without pronase, prote...

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Veröffentlicht in:	FEMS immunology and medical microbiology 2000-01, Vol.27 (1), p.17-22
Hauptverfasser:	Jin, L.Z., Marquardt, Ronald R., Baidoo, Samuel K., Frohlich, Andrew A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adhesins, Escherichia coli - metabolism Adhesion Affinity chromatography Animals Antigens, Bacterial Antigens, Surface - metabolism Bacterial Adhesion Bacteriology Biological and medical sciences Chromatography, Affinity Chromatography, Gel E coli Electrophoresis Escherichia coli Escherichia coli - growth & development Escherichia coli - metabolism Escherichia coli Infections - microbiology Escherichia coli Infections - veterinary Escherichia coli K88 Escherichia coli Proteins Fimbriae Proteins Fimbriae, Bacterial - metabolism Fundamental and applied biological sciences. Psychology Gel electrophoresis Glycoproteins Hydrogen-Ion Concentration Intestinal Mucosa - microbiology Intestinal Mucosa - secretion Intestinal mucus receptor Intestine Intestine, Small - microbiology Microbiology Mucus Mucus - chemistry Mucus - metabolism Mucus - microbiology Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Pili Polyacrylamide Pronase Proteinase Proteolysis Proteolytic enzymes Receptors Receptors, Immunologic - chemistry Receptors, Immunologic - isolation & purification Receptors, Immunologic - metabolism Small intestine Sodium Sodium dodecyl sulfate Sodium lauryl sulfate Sugar Swine Swine Diseases - microbiology Trypsin
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