Stage IIa Cervix Carcinoma with Metastasis to the Heart: Report of a Case with Immunohistochemistry, Flow Cytometry, and Virology Findings
Objective. The aim of this study was to report a stage IIa squamous cell cervix carcinoma with intraperitoneal carcinomatosis and metastasis to the heart in a 50-year-old woman and to study the original tumor for expression of oncogenes and tumor suppressor gene proteins, for DNA ploidy, and for hum...
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| Veröffentlicht in: | Gynecologic oncology 2000-01, Vol.76 (1), p.133-138 |
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| creator | Sergi, Consolato Magener, Achim Ehemann, Volker De Villiers, Ethel-Michele Sinn, Hans-Peter |
| description | Objective. The aim of this study was to report a stage IIa squamous cell cervix carcinoma with intraperitoneal carcinomatosis and metastasis to the heart in a 50-year-old woman and to study the original tumor for expression of oncogenes and tumor suppressor gene proteins, for DNA ploidy, and for human papillomavirus (HPV) infection.
Methods. Clinical course, histopathology of the original tumor, and autopsy record were rewieved. The original tumor was analyzed for expression of CD44 variant 6, p16, p21, p53, retinoblastoma (Rb), and c-erb-2. DNA flow cytometry was performed on tissue samples from the original tumor and from the heart. Sequences of the HPV genome on cervical and cardiac tissue samples were amplified by polymerase chain reaction.
Results. Immunohistochemical analysis showed expression of CD44v6 and p16. No expression of p21, Rb, c-erb-B2, and p53 was seen. DNA flow cytometry of the original cervical tumor showed a DNA index (DI) of 1.0. DNA flow cytometry of tissue samples from the posterior wall and from the right ventricle of the heart showed two different aneuploid cell populations with DI of 1.6 and 2.2, respectively. HPV gene sequences were identified neither in the original tumor nor in the heart.
Conclusions. To our knowledge, this is the first case of cervix carcinoma with metastasis to the heart with immunohistochemistry, flow cytometry, and virology findings. |
| doi_str_mv | 10.1006/gyno.1999.5650 |
| format | Article |
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Methods. Clinical course, histopathology of the original tumor, and autopsy record were rewieved. The original tumor was analyzed for expression of CD44 variant 6, p16, p21, p53, retinoblastoma (Rb), and c-erb-2. DNA flow cytometry was performed on tissue samples from the original tumor and from the heart. Sequences of the HPV genome on cervical and cardiac tissue samples were amplified by polymerase chain reaction.
Results. Immunohistochemical analysis showed expression of CD44v6 and p16. No expression of p21, Rb, c-erb-B2, and p53 was seen. DNA flow cytometry of the original cervical tumor showed a DNA index (DI) of 1.0. DNA flow cytometry of tissue samples from the posterior wall and from the right ventricle of the heart showed two different aneuploid cell populations with DI of 1.6 and 2.2, respectively. HPV gene sequences were identified neither in the original tumor nor in the heart.
Conclusions. To our knowledge, this is the first case of cervix carcinoma with metastasis to the heart with immunohistochemistry, flow cytometry, and virology findings.</description><identifier>ISSN: 0090-8258</identifier><identifier>EISSN: 1095-6859</identifier><identifier>DOI: 10.1006/gyno.1999.5650</identifier><identifier>PMID: 10620458</identifier><identifier>CODEN: GYNOA3</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Biological and medical sciences ; Carcinoma, Squamous Cell - genetics ; Carcinoma, Squamous Cell - secondary ; cervix ; Female ; Female genital diseases ; Flow Cytometry ; Gene Expression Regulation, Neoplastic ; Gynecology. Andrology. Obstetrics ; heart ; Heart Neoplasms - genetics ; Heart Neoplasms - secondary ; HPV ; Humans ; Immunohistochemistry ; Medical sciences ; metastasis ; Middle Aged ; oncogene ; Oncogenes - genetics ; Papillomaviridae ; Papillomavirus Infections - immunology ; Papillomavirus Infections - virology ; Ploidies ; single-cell cytometry ; squamous cell carcinoma ; tumor suppressor gene ; Tumors ; Uterine Cervical Neoplasms - genetics ; Uterine Cervical Neoplasms - pathology</subject><ispartof>Gynecologic oncology, 2000-01, Vol.76 (1), p.133-138</ispartof><rights>2000 Academic Press</rights><rights>2000 INIST-CNRS</rights><rights>Copyright 2000 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c369t-ffe832dae56f03703791f30a95a51ffaaab5e1479845b7454c8f362bba8804ac3</citedby><cites>FETCH-LOGICAL-c369t-ffe832dae56f03703791f30a95a51ffaaab5e1479845b7454c8f362bba8804ac3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/gyno.1999.5650$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,4022,27921,27922,27923,45993</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1242665$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10620458$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sergi, Consolato</creatorcontrib><creatorcontrib>Magener, Achim</creatorcontrib><creatorcontrib>Ehemann, Volker</creatorcontrib><creatorcontrib>De Villiers, Ethel-Michele</creatorcontrib><creatorcontrib>Sinn, Hans-Peter</creatorcontrib><title>Stage IIa Cervix Carcinoma with Metastasis to the Heart: Report of a Case with Immunohistochemistry, Flow Cytometry, and Virology Findings</title><title>Gynecologic oncology</title><addtitle>Gynecol Oncol</addtitle><description>Objective. The aim of this study was to report a stage IIa squamous cell cervix carcinoma with intraperitoneal carcinomatosis and metastasis to the heart in a 50-year-old woman and to study the original tumor for expression of oncogenes and tumor suppressor gene proteins, for DNA ploidy, and for human papillomavirus (HPV) infection.
Methods. Clinical course, histopathology of the original tumor, and autopsy record were rewieved. The original tumor was analyzed for expression of CD44 variant 6, p16, p21, p53, retinoblastoma (Rb), and c-erb-2. DNA flow cytometry was performed on tissue samples from the original tumor and from the heart. Sequences of the HPV genome on cervical and cardiac tissue samples were amplified by polymerase chain reaction.
Results. Immunohistochemical analysis showed expression of CD44v6 and p16. No expression of p21, Rb, c-erb-B2, and p53 was seen. DNA flow cytometry of the original cervical tumor showed a DNA index (DI) of 1.0. DNA flow cytometry of tissue samples from the posterior wall and from the right ventricle of the heart showed two different aneuploid cell populations with DI of 1.6 and 2.2, respectively. HPV gene sequences were identified neither in the original tumor nor in the heart.
Conclusions. To our knowledge, this is the first case of cervix carcinoma with metastasis to the heart with immunohistochemistry, flow cytometry, and virology findings.</description><subject>Biological and medical sciences</subject><subject>Carcinoma, Squamous Cell - genetics</subject><subject>Carcinoma, Squamous Cell - secondary</subject><subject>cervix</subject><subject>Female</subject><subject>Female genital diseases</subject><subject>Flow Cytometry</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>heart</subject><subject>Heart Neoplasms - genetics</subject><subject>Heart Neoplasms - secondary</subject><subject>HPV</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Medical sciences</subject><subject>metastasis</subject><subject>Middle Aged</subject><subject>oncogene</subject><subject>Oncogenes - genetics</subject><subject>Papillomaviridae</subject><subject>Papillomavirus Infections - immunology</subject><subject>Papillomavirus Infections - virology</subject><subject>Ploidies</subject><subject>single-cell cytometry</subject><subject>squamous cell carcinoma</subject><subject>tumor suppressor gene</subject><subject>Tumors</subject><subject>Uterine Cervical Neoplasms - genetics</subject><subject>Uterine Cervical Neoplasms - pathology</subject><issn>0090-8258</issn><issn>1095-6859</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE2LFDEQhoMo7uzq1aPkIJ7ssfojPYm3pXHcgRXBr2tTk67MRLo7Y5LZtf-Cv3oz9oBehIKi4KmXl4exFzksc4D67W4a3TJXSi1FLeARW-SgRFZLoR6zBYCCTBZCXrDLEH4AQAl58ZRd5FAXUAm5YL-_RNwR32yQN-Tv7C_eoNd2dAPyexv3_CNFDGls4NHxuCd-Q-jjO_6ZDs5H7gxPrxhoxjfDcBzd3obo9J6GtP30hq97d8-bKbqB_tw4dvy79a53u4mv7djZcReesScG-0DPz_uKfVu__9rcZLefPmya69tMl7WKmTEky6JDErWBcpVG5aYEVAJFbgwibgXl1UrJSmxXlai0NGVdbLcoJVSoyyv2es49ePfzSCG2qaamvseR3DG0K5AgKigTuJxB7V0Inkx78HZAP7U5tCf77cl-e7Lfnuynh5fn5ON2oO4ffNadgFdnAIPG3ngctQ1_uaIq6lokTM4YJQ13lnwbtKVRU2c96dh2zv6vwgPp_aI-</recordid><startdate>200001</startdate><enddate>200001</enddate><creator>Sergi, Consolato</creator><creator>Magener, Achim</creator><creator>Ehemann, Volker</creator><creator>De Villiers, Ethel-Michele</creator><creator>Sinn, Hans-Peter</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200001</creationdate><title>Stage IIa Cervix Carcinoma with Metastasis to the Heart: Report of a Case with Immunohistochemistry, Flow Cytometry, and Virology Findings</title><author>Sergi, Consolato ; Magener, Achim ; Ehemann, Volker ; De Villiers, Ethel-Michele ; Sinn, Hans-Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c369t-ffe832dae56f03703791f30a95a51ffaaab5e1479845b7454c8f362bba8804ac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Biological and medical sciences</topic><topic>Carcinoma, Squamous Cell - genetics</topic><topic>Carcinoma, Squamous Cell - secondary</topic><topic>cervix</topic><topic>Female</topic><topic>Female genital diseases</topic><topic>Flow Cytometry</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>heart</topic><topic>Heart Neoplasms - genetics</topic><topic>Heart Neoplasms - secondary</topic><topic>HPV</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Medical sciences</topic><topic>metastasis</topic><topic>Middle Aged</topic><topic>oncogene</topic><topic>Oncogenes - genetics</topic><topic>Papillomaviridae</topic><topic>Papillomavirus Infections - immunology</topic><topic>Papillomavirus Infections - virology</topic><topic>Ploidies</topic><topic>single-cell cytometry</topic><topic>squamous cell carcinoma</topic><topic>tumor suppressor gene</topic><topic>Tumors</topic><topic>Uterine Cervical Neoplasms - genetics</topic><topic>Uterine Cervical Neoplasms - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sergi, Consolato</creatorcontrib><creatorcontrib>Magener, Achim</creatorcontrib><creatorcontrib>Ehemann, Volker</creatorcontrib><creatorcontrib>De Villiers, Ethel-Michele</creatorcontrib><creatorcontrib>Sinn, Hans-Peter</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Gynecologic oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sergi, Consolato</au><au>Magener, Achim</au><au>Ehemann, Volker</au><au>De Villiers, Ethel-Michele</au><au>Sinn, Hans-Peter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stage IIa Cervix Carcinoma with Metastasis to the Heart: Report of a Case with Immunohistochemistry, Flow Cytometry, and Virology Findings</atitle><jtitle>Gynecologic oncology</jtitle><addtitle>Gynecol Oncol</addtitle><date>2000-01</date><risdate>2000</risdate><volume>76</volume><issue>1</issue><spage>133</spage><epage>138</epage><pages>133-138</pages><issn>0090-8258</issn><eissn>1095-6859</eissn><coden>GYNOA3</coden><abstract>Objective. The aim of this study was to report a stage IIa squamous cell cervix carcinoma with intraperitoneal carcinomatosis and metastasis to the heart in a 50-year-old woman and to study the original tumor for expression of oncogenes and tumor suppressor gene proteins, for DNA ploidy, and for human papillomavirus (HPV) infection.
Methods. Clinical course, histopathology of the original tumor, and autopsy record were rewieved. The original tumor was analyzed for expression of CD44 variant 6, p16, p21, p53, retinoblastoma (Rb), and c-erb-2. DNA flow cytometry was performed on tissue samples from the original tumor and from the heart. Sequences of the HPV genome on cervical and cardiac tissue samples were amplified by polymerase chain reaction.
Results. Immunohistochemical analysis showed expression of CD44v6 and p16. No expression of p21, Rb, c-erb-B2, and p53 was seen. DNA flow cytometry of the original cervical tumor showed a DNA index (DI) of 1.0. DNA flow cytometry of tissue samples from the posterior wall and from the right ventricle of the heart showed two different aneuploid cell populations with DI of 1.6 and 2.2, respectively. HPV gene sequences were identified neither in the original tumor nor in the heart.
Conclusions. To our knowledge, this is the first case of cervix carcinoma with metastasis to the heart with immunohistochemistry, flow cytometry, and virology findings.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>10620458</pmid><doi>10.1006/gyno.1999.5650</doi><tpages>6</tpages></addata></record> |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Biological and medical sciences Carcinoma, Squamous Cell - genetics Carcinoma, Squamous Cell - secondary cervix Female Female genital diseases Flow Cytometry Gene Expression Regulation, Neoplastic Gynecology. Andrology. Obstetrics heart Heart Neoplasms - genetics Heart Neoplasms - secondary HPV Humans Immunohistochemistry Medical sciences metastasis Middle Aged oncogene Oncogenes - genetics Papillomaviridae Papillomavirus Infections - immunology Papillomavirus Infections - virology Ploidies single-cell cytometry squamous cell carcinoma tumor suppressor gene Tumors Uterine Cervical Neoplasms - genetics Uterine Cervical Neoplasms - pathology |
| title | Stage IIa Cervix Carcinoma with Metastasis to the Heart: Report of a Case with Immunohistochemistry, Flow Cytometry, and Virology Findings |
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